Restriction map for
pF1KSDA1264
(Circular) (Six-base) MAPSORT of: pF1KSDA1264.seq Check: 5313 from: 1 to: 5294
pF1KSDA1264 5294 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 2332 2332
Size: 5294
Acc65I G'GTAC_C
Cuts at: 1253 2310 1253
Size: 1057 4237
AclI AA'CG_TT
Cuts at: 5134 5134
Size: 5294
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2120 3119 3551 4298 4566 2120
Size: 999 432 747 268 2848
Fragments arranged by size:
2848 999 747 432 268
AflIII A'CryG_T
Cuts at: 1649 2646 3750 4727 1649
Size: 997 1104 977 2216
Fragments arranged by size:
2216 1104 997 977
AgeI A'CCGG_T
Cuts at: 3682 3682
Size: 5294
AhdI GACnn_n'nnGTC
Cuts at: 2004 2004
Size: 5294
AlwNI CAG_nnn'CTG
Cuts at: 1165 2599 4166 1165
Size: 1434 1567 2293
Fragments arranged by size:
2293 1567 1434
ApaI G_GGCC'C
Cuts at: 1186 1186
Size: 5294
ApaLI G'TGCA_C
Cuts at: 4064 4064
Size: 5294
ApoI r'AATT_y
Cuts at: 2298 4647 4903 2298
Size: 2349 256 2689
Fragments arranged by size:
2689 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5294
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5294
AvaI C'yCGr_G
Cuts at: 792 993 2011 2314 792
Size: 201 1018 303 3772
Fragments arranged by size:
3772 1018 303 201
BamHI G'GATC_C
Cuts at: 594 2087 2319 594
Size: 1493 232 3569
Fragments arranged by size:
3569 1493 232
BanI G'GyrC_C
Cuts at: 1253 1711 1914 2310 2724 2972 3007 1253
Size: 458 203 396 414 248 35 3540
Fragments arranged by size:
3540 458 414 396 248 203 35
BanII G_rGCy'C
Cuts at: 1097 1186 1333 1378 1682 1791 2048 2180 2308
Size: 89 147 45 304 109 257 132 128
Cuts at: 2308 3338 1097
Size: 1030 3053
Fragments arranged by size:
3053 1030 304 257 147 132 128 109
89 45
BbeI G_GCGC'C
Cuts at: 2728 2976 2728
Size: 248 5046
BbsI GAAGACnn'nnnn_
Cuts at: 334 926 334
Size: 592 4702
BbvCI CC'TCA_GC
Cuts at: 959 1144 1336 959
Size: 185 192 4917
Fragments arranged by size:
4917 192 185
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 520 554 520
Size: 34 5260
BciVI GTATCCnnnnn_n'
Cuts at: 3192 3953 5294 3192
Size: 761 1341 3192
Fragments arranged by size:
3192 1341 761
BclI T'GATC_A
Cuts at: 1613 2814 1613
Size: 1201 4093
BfrBI ATG'CAT
Cuts at: 299 1517 299
Size: 1218 4076
BglII A'GATC_T
Cuts at: 2809 4846 2809
Size: 2037 3257
BlpI GC'TnA_GC
Cuts at: 2406 2406
Size: 5294
Bme1580I G_kGCm'C
Cuts at: 638 1186 2919 3012 4068 638
Size: 548 1733 93 1056 1864
Fragments arranged by size:
1864 1733 1056 548 93
BmrI ACTGGGnnnn_n'
Cuts at: 203 507 2506 2587 2922 5066 203
Size: 304 1999 81 335 2144 431
Fragments arranged by size:
2144 1999 431 335 304 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3661 3661
Size: 5294
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 447 985 1383 2056 447
Size: 538 398 673 3685
Fragments arranged by size:
3685 673 538 398
Bpu10I CC'TnA_GC
Cuts at: 959 1144 1327 1336 959
Size: 185 183 9 4917
Fragments arranged by size:
4917 185 183 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 1212 1883 2470 3349 3841 4139 754
Size: 458 671 587 879 492 298 1909
Fragments arranged by size:
1909 879 671 587 492 458 298
BsaI GGTCTCn'nnnn_
Cuts at: 1448 4998 1448
Size: 3550 1744
BsaAI yAC'GTr
Cuts at: 639 2286 2647 639
Size: 1647 361 3286
Fragments arranged by size:
3286 1647 361
BsaBI GATnn'nnATC
Cuts at: 6 593 1124 2808 4845 6
Size: 587 531 1684 2037 455
Fragments arranged by size:
2037 1684 587 531 455
BsaHI Gr'CG_yC
Cuts at: 2725 2973 3675 5171 2725
Size: 248 702 1496 2848
Fragments arranged by size:
2848 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1571 2483 3004 3682 3956 4103 4514 1571
Size: 912 521 678 274 147 411 2351
Fragments arranged by size:
2351 912 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1315 1345 1315
Size: 30 5264
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1368 1371 2197 1368
Size: 3 826 4465
Fragments arranged by size:
4465 826 3
BseYI C'CCAG_C
Cuts at: 233 901 1366 1370 2719 3477 4054 4550 233
Size: 668 465 4 1349 758 577 496 977
Fragments arranged by size:
1349 977 758 668 577 496 465 4
BsiEI CG_ry'CG
Cuts at: 104 554 1128 2836 2882 4090 104
Size: 450 574 1708 46 1208 1308
Fragments arranged by size:
1708 1308 1208 574 450 46
BsiHKAI G_wGCw'C
Cuts at: 862 1134 1333 1682 1791 1899 2144 2308 3086
Size: 272 199 349 109 108 245 164 778
Cuts at: 3086 3276 4068 862
Size: 190 792 2088
Fragments arranged by size:
2088 792 778 349 272 245 199 190
164 109 108
BsmI GAATG_Cn'
Cuts at: 1106 1106
Size: 5294
BsmBI CGTCTCn'nnnn_
Cuts at: 1578 1578
Size: 5294
Bsp1286I G_dGCh'C
Cuts at: 638 862 1097 1134 1186 1333 1378 1682 1791
Size: 224 235 37 52 147 45 304 109
Cuts at: 1791 1899 2048 2144 2180 2308 2919 3012 3086
Size: 108 149 96 36 128 611 93 74
Cuts at: 3086 3276 3338 4068 638
Size: 190 62 730 1864
Fragments arranged by size:
1864 730 611 304 235 224 190 149
147 128 109 108 96 93 74 62
52 45 37 36
BspEI T'CCGG_A
Cuts at: 1571 4514 1571
Size: 2943 2351
BspHI T'CATG_A
Cuts at: 2261 4470 2261
Size: 2209 3085
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2224 2344 2558 2860 3241 2224
Size: 120 214 302 381 4277
Fragments arranged by size:
4277 381 302 214 120
BsrBI CCG'CTC
Cuts at: 50 3586 3640 5124 5289 50
Size: 3536 54 1484 165 55
Fragments arranged by size:
3536 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3206 3206
Size: 5294
BsrFI r'CCGG_y
Cuts at: 223 1504 3292 3473 3682 223
Size: 1281 1788 181 209 1835
Fragments arranged by size:
1835 1788 1281 209 181
BsrGI T'GTAC_A
Cuts at: 1652 1652
Size: 5294
BssSI C'ACGA_G
Cuts at: 3565 3923 3565
Size: 358 4936
BstAPI GCAn_nnn'nTGC
Cuts at: 1514 2671 1514
Size: 1157 4137
BstBI TT'CG_AA
Cuts at: 110 3655 110
Size: 3545 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 315 315
Size: 5294
BstYI r'GATC_y
Cuts at: 1 594 2087 2319 2786 2809 3144 3390 4402
Size: 593 1493 232 467 23 335 246 1012
Cuts at: 4402 4488 4500 4846 1
Size: 86 12 346 449
Fragments arranged by size:
1493 1012 593 467 449 346 335 246
232 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 424 1961 424
Size: 1537 3757
BtgI C'CryG_G
Cuts at: 468 1313 468
Size: 845 4449
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1503 1688 1874 2092 3244 3425 3588 1503
Size: 185 186 218 1152 181 163 3209
Fragments arranged by size:
3209 1152 218 186 185 181 163
BtsI GCAGTG_nn'
Cuts at: 244 1559 2537 244
Size: 1315 978 3001
Fragments arranged by size:
3001 1315 978
DraI TTT'AAA
Cuts at: 1038 2293 1038
Size: 1255 4039
DraIII CAC_nnn'GTG
Cuts at: 1103 1103
Size: 5294
DrdI GACnn_nn'nnGTC
Cuts at: 3000 3858 3000
Size: 858 4436
EaeI y'GGCC_r
Cuts at: 263 1472 1863 2879 3053 3444 3471 4816 263
Size: 1209 391 1016 174 391 27 1345 741
Fragments arranged by size:
1345 1209 1016 741 391 391 174 27
EagI C'GGCC_G
Cuts at: 2879 2879
Size: 5294
EarI CTCTTCn'nnn_
Cuts at: 565 1915 3317 3527 565
Size: 1350 1402 210 2332
Fragments arranged by size:
2332 1402 1350 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3502 3812 3958 5104 3502
Size: 310 146 1146 3692
Fragments arranged by size:
3692 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1331 1680 1789 2306 1331
Size: 349 109 517 4319
Fragments arranged by size:
4319 517 349 109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 447 985 1383 2056 2120 3119 3551 4298 4566
Size: 538 398 673 64 999 432 747 268
Cuts at: 4566 447
Size: 1175
Fragments arranged by size:
1175 999 747 673 538 432 398 268
64
EcoNI CCTnn'n_nnAGG
Cuts at: 382 1176 2125 382
Size: 794 949 3551
Fragments arranged by size:
3551 949 794
EcoO109I rG'GnC_Cy
Cuts at: 898 1182 1183 2118 2433 898
Size: 284 1 935 315 3759
Fragments arranged by size:
3759 935 315 284 1
EcoRI G'AATT_C
Cuts at: 2298 2298
Size: 5294
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3313 3313
Size: 5294
FspI TGC'GCA
Cuts at: 3075 3075
Size: 5294
HaeII r_GCGC'y
Cuts at: 2728 2976 3998 4977 2728
Size: 248 1022 979 3045
Fragments arranged by size:
3045 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 385 417 709 741 942 974 1990 2022 3136
Size: 32 292 32 201 32 1016 32 1114
Cuts at: 3136 3166 3168 3198 385
Size: 30 2 30 2481
Fragments arranged by size:
2481 1114 1016 292 201 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1647 2333 1647
Size: 686 4608
KasI G'GCGC_C
Cuts at: 2724 2972 2724
Size: 248 5046
KpnI G_GTAC'C
Cuts at: 1257 2314 1257
Size: 1057 4237
MluI A'CGCG_T
Cuts at: 4727 4727
Size: 5294
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 194 440 1409 3965 4149 194
Size: 246 969 2556 184 1339
Fragments arranged by size:
2556 1339 969 246 184
MscI TGG'CCA
Cuts at: 1474 3055 1474
Size: 1581 3713
MslI CAynn'nnrTG
Cuts at: 403 1637 1741 3283 3410 403
Size: 1234 104 1542 127 2287
Fragments arranged by size:
2287 1542 1234 127 104
MspA1I CmG'CkG
Cuts at: 315 854 1370 1437 2405 2719 3079 4092 4337
Size: 539 516 67 968 314 360 1013 245
Cuts at: 4337 315
Size: 1272
Fragments arranged by size:
1272 1013 968 539 516 360 314 245
67
NaeI GCC'GGC
Cuts at: 3475 3475
Size: 5294
NarI GG'CG_CC
Cuts at: 2725 2973 2725
Size: 248 5046
NgoMIV G'CCGG_C
Cuts at: 3473 3473
Size: 5294
NsiI A_TGCA'T
Cuts at: 301 1519 301
Size: 1218 4076
NspI r_CATG'y
Cuts at: 1653 2347 2847 3754 1653
Size: 694 500 907 3193
Fragments arranged by size:
3193 907 694 500
PasI CC'CwG_GG
Cuts at: 1178 1460 1178
Size: 282 5012
PciI A'CATG_T
Cuts at: 1649 3750 1649
Size: 2101 3193
PfoI T'CCnGG_A
Cuts at: 1001 2090 1001
Size: 1089 4205
PmeI GTTT'AAAC
Cuts at: 2293 2293
Size: 5294
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4433 4465 4433
Size: 32 5262
PpuMI rG'GwC_Cy
Cuts at: 898 2118 898
Size: 1220 4074
PspOMI G'GGCC_C
Cuts at: 1182 1182
Size: 5294
PspXI vC'TCGA_Gb
Cuts at: 792 792
Size: 5294
PstI C_TGCA'G
Cuts at: 846 1292 1436 2341 3026 846
Size: 446 144 905 685 3114
Fragments arranged by size:
3114 905 685 446 144
PvuI CG_AT'CG
Cuts at: 104 1128 104
Size: 1024 4270
PvuII CAG'CTG
Cuts at: 315 1370 1437 2719 3079 315
Size: 1055 67 1282 360 2530
Fragments arranged by size:
2530 1282 1055 360 67
RsrII CG'GwC_CG
Cuts at: 3489 3489
Size: 5294
SacI G_AGCT'C
Cuts at: 1333 1682 1791 2308 1333
Size: 349 109 517 4319
Fragments arranged by size:
4319 517 349 109
SalI G'TCGA_C
Cuts at: 2331 2331
Size: 5294
SanDI GG'GwC_CC
Cuts at: 898 898
Size: 5294
SapI GCTCTTCn'nnn_
Cuts at: 3317 3527 3317
Size: 210 5084
SbfI CC_TGCA'GG
Cuts at: 2341 2341
Size: 5294
ScaI AGT'ACT
Cuts at: 4811 4811
Size: 5294
SfcI C'TryA_G
Cuts at: 33 842 1288 1432 2337 3022 4015 4206 33
Size: 809 446 144 905 685 993 191 1121
Fragments arranged by size:
1121 993 905 809 685 446 191 144
SfoI GGC'GCC
Cuts at: 2726 2974 2726
Size: 248 5046
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5294
SmaI CCC'GGG
Cuts at: 2316 2316
Size: 5294
SmlI C'TyrA_G
Cuts at: 769 792 1227 1898 2449 3364 3856 4118 769
Size: 23 435 671 551 915 492 262 1945
Fragments arranged by size:
1945 915 671 551 492 435 262 23
SnaBI TAC'GTA
Cuts at: 2286 2286
Size: 5294
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5294
SphI G_CATG'C
Cuts at: 2347 2847 2347
Size: 500 4794
StuI AGG'CCT
Cuts at: 664 664
Size: 5294
StyI C'CwwG_G
Cuts at: 863 1050 1809 2428 2782 863
Size: 187 759 619 354 3375
Fragments arranged by size:
3375 759 619 354 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 321 456 568 1674 2877 3010 3469 3678 321
Size: 135 112 1106 1203 133 459 209 1937
Fragments arranged by size:
1937 1203 1106 459 209 135 133 112
TatI w'GTAC_w
Cuts at: 560 1304 1652 4809 560
Size: 744 348 3157 1045
Fragments arranged by size:
3157 1045 744 348
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1124 1156 4811 4843 1124
Size: 32 3655 32 1575
Fragments arranged by size:
3655 1575 32 32
Tth111I GACn'n_nGTC
Cuts at: 3091 3091
Size: 5294
XbaI T'CTAG_A
Cuts at: 835 1701 2325 835
Size: 866 624 3804
Fragments arranged by size:
3804 866 624
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 870 1246 1467 2789 870
Size: 376 221 1322 3375
Fragments arranged by size:
3375 1322 376 221
XhoI C'TCGA_G
Cuts at: 792 792
Size: 5294
XmaI C'CCGG_G
Cuts at: 2314 2314
Size: 5294
XmnI GAAnn'nnTTC
Cuts at: 1418 1418
Size: 5294
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NsiI NspI PasI PciI PfoI PmeI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BglI BmgBI BmtI BsgI BsiWI BssHII
BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI PacI
PflMI PmlI PshAI PsiI PsrI SacII SexAI SfiI
SgrAI SrfI SspI SwaI ZraI