Restriction map for
pF1KSDA1266
(Circular) (Six-base) MAPSORT of: pF1KSDA1266.seq Check: 4936 from: 1 to: 4748
pF1KSDA1266 4748 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 1786 1786
Size: 4748
Acc65I G'GTAC_C
Cuts at: 1764 1764
Size: 4748
AclI AA'CG_TT
Cuts at: 4588 4588
Size: 4748
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 800 2573 3005 3752 4020 800
Size: 1773 432 747 268 1528
Fragments arranged by size:
1773 1528 747 432 268
AflIII A'CryG_T
Cuts at: 890 1277 2100 3204 4181 890
Size: 387 823 1104 977 1457
Fragments arranged by size:
1457 1104 977 823 387
AgeI A'CCGG_T
Cuts at: 3136 3136
Size: 4748
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 644 644
Size: 4748
AlwNI CAG_nnn'CTG
Cuts at: 778 946 2053 3620 778
Size: 168 1107 1567 1906
Fragments arranged by size:
1906 1567 1107 168
ApaI G_GGCC'C
Cuts at: 1138 1138
Size: 4748
ApaLI G'TGCA_C
Cuts at: 3518 3518
Size: 4748
ApoI r'AATT_y
Cuts at: 1384 1752 4101 4357 1384
Size: 368 2349 256 1775
Fragments arranged by size:
2349 1775 368 256
AseI AT'TA_AT
Cuts at: 20 1454 20
Size: 1434 3314
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4748
AvaI C'yCGr_G
Cuts at: 644 1768 644
Size: 1124 3624
BamHI G'GATC_C
Cuts at: 504 1773 504
Size: 1269 3479
BanI G'GyrC_C
Cuts at: 1764 2178 2426 2461 1764
Size: 414 248 35 4051
Fragments arranged by size:
4051 414 248 35
BanII G_rGCy'C
Cuts at: 585 1138 1762 2792 585
Size: 553 624 1030 2541
Fragments arranged by size:
2541 1030 624 553
BbeI G_GCGC'C
Cuts at: 2182 2430 2182
Size: 248 4500
BbsI GAAGACnn'nnnn_
Cuts at: 1348 1348
Size: 4748
BciVI GTATCCnnnnn_n'
Cuts at: 333 666 2646 3407 4748 333
Size: 333 1980 761 1341 333
Fragments arranged by size:
1980 1341 761 333 333
BclI T'GATC_A
Cuts at: 2268 2268
Size: 4748
BglI GCCn_nnn'nGGC
Cuts at: 1471 1471
Size: 4748
BglII A'GATC_T
Cuts at: 2263 4300 2263
Size: 2037 2711
BlpI GC'TnA_GC
Cuts at: 1860 1860
Size: 4748
Bme1580I G_kGCm'C
Cuts at: 1138 2373 2466 3522 1138
Size: 1235 93 1056 2364
Fragments arranged by size:
2364 1235 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1209 1328 1960 2041 2376 4520 1209
Size: 119 632 81 335 2144 1437
Fragments arranged by size:
2144 1437 632 335 119 81
BmtI G_CTAG'C
Cuts at: 788 788
Size: 4748
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3115 3115
Size: 4748
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1924 2803 3295 3593 1924
Size: 879 492 298 3079
Fragments arranged by size:
3079 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4452 4452
Size: 4748
BsaAI yAC'GTr
Cuts at: 559 1278 1721 1740 2101 559
Size: 719 443 19 361 3206
Fragments arranged by size:
3206 719 443 361 19
BsaBI GATnn'nnATC
Cuts at: 6 2262 4299 6
Size: 2256 2037 455
Fragments arranged by size:
2256 2037 455
BsaHI Gr'CG_yC
Cuts at: 2179 2427 3129 4625 2179
Size: 248 702 1496 2302
Fragments arranged by size:
2302 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1937 2458 3136 3410 3557 3968 1937
Size: 521 678 274 147 411 2717
Fragments arranged by size:
2717 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 725 754 755 784 1074 1104 725
Size: 29 1 29 290 30 4369
Fragments arranged by size:
4369 290 30 29 29 1
BseYI C'CCAG_C
Cuts at: 2173 2931 3508 4004 2173
Size: 758 577 496 2917
Fragments arranged by size:
2917 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 303 303
Size: 4748
BsiEI CG_ry'CG
Cuts at: 104 1441 2290 2336 3544 104
Size: 1337 849 46 1208 1308
Fragments arranged by size:
1337 1308 1208 849 46
BsiHKAI G_wGCw'C
Cuts at: 1762 2540 2730 3522 1762
Size: 778 190 792 2988
Fragments arranged by size:
2988 792 778 190
BsmI GAATG_Cn'
Cuts at: 1307 1670 1307
Size: 363 4385
Bsp1286I G_dGCh'C
Cuts at: 585 1138 1762 2373 2466 2540 2730 2792 3522
Size: 553 624 611 93 74 190 62 730
Cuts at: 3522 585
Size: 1811
Fragments arranged by size:
1811 730 624 611 553 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3968 3968
Size: 4748
BspHI T'CATG_A
Cuts at: 3924 3924
Size: 4748
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1798 2012 2314 2695 1798
Size: 214 302 381 3851
Fragments arranged by size:
3851 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3040 3094 4578 4743 50
Size: 2990 54 1484 165 55
Fragments arranged by size:
2990 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2660 2660
Size: 4748
BsrFI r'CCGG_y
Cuts at: 2746 2927 3136 2746
Size: 181 209 4358
Fragments arranged by size:
4358 209 181
BssSI C'ACGA_G
Cuts at: 1262 3019 3377 1262
Size: 1757 358 2633
Fragments arranged by size:
2633 1757 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1302 1480 2125 1302
Size: 178 645 3925
Fragments arranged by size:
3925 645 178
BstBI TT'CG_AA
Cuts at: 110 3109 110
Size: 2999 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 585 585
Size: 4748
BstYI r'GATC_y
Cuts at: 1 504 1568 1773 2240 2263 2598 2844 3856
Size: 503 1064 205 467 23 335 246 1012
Cuts at: 3856 3942 3954 4300 1
Size: 86 12 346 449
Fragments arranged by size:
1064 1012 503 467 449 346 335 246
205 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2698 2879 3042 2698
Size: 181 163 4404
Fragments arranged by size:
4404 181 163
BtsI GCAGTG_nn'
Cuts at: 291 711 1156 1343 1991 291
Size: 420 445 187 648 3048
Fragments arranged by size:
3048 648 445 420 187
DraI TTT'AAA
Cuts at: 1747 1747
Size: 4748
DrdI GACnn_nn'nnGTC
Cuts at: 2454 3312 2454
Size: 858 3890
EaeI y'GGCC_r
Cuts at: 1438 2333 2507 2898 2925 4270 1438
Size: 895 174 391 27 1345 1916
Fragments arranged by size:
1916 1345 895 391 174 27
EagI C'GGCC_G
Cuts at: 1438 2333 1438
Size: 895 3853
EarI CTCTTCn'nnn_
Cuts at: 791 1220 2771 2981 791
Size: 429 1551 210 2558
Fragments arranged by size:
2558 1551 429 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2956 3266 3412 4558 2956
Size: 310 146 1146 3146
Fragments arranged by size:
3146 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1760 1760
Size: 4748
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 800 2573 3005 3752 4020 800
Size: 1773 432 747 268 1528
Fragments arranged by size:
1773 1528 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1076 1076
Size: 4748
EcoO109I rG'GnC_Cy
Cuts at: 415 736 1134 1257 1887 415
Size: 321 398 123 630 3276
Fragments arranged by size:
3276 630 398 321 123
EcoRI G'AATT_C
Cuts at: 1752 1752
Size: 4748
EcoRV GAT'ATC
Cuts at: 424 424
Size: 4748
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 865 2767 865
Size: 1902 2846
FspI TGC'GCA
Cuts at: 2529 2529
Size: 4748
HaeII r_GCGC'y
Cuts at: 2182 2430 3452 4431 2182
Size: 248 1022 979 2499
Fragments arranged by size:
2499 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 148 180 295 327 754 786 1219 1251 2590
Size: 32 115 32 427 32 433 32 1339
Cuts at: 2590 2620 2622 2652 148
Size: 30 2 30 2244
Fragments arranged by size:
2244 1339 433 427 115 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1787 1787
Size: 4748
HindIII A'AGCT_T
Cuts at: 1352 1352
Size: 4748
KasI G'GCGC_C
Cuts at: 2178 2426 2178
Size: 248 4500
KpnI G_GTAC'C
Cuts at: 1768 1768
Size: 4748
MluI A'CGCG_T
Cuts at: 4181 4181
Size: 4748
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3419 3603 3419
Size: 184 4564
MscI TGG'CCA
Cuts at: 2509 2509
Size: 4748
MslI CAynn'nnrTG
Cuts at: 626 692 1700 2737 2864 626
Size: 66 1008 1037 127 2510
Fragments arranged by size:
2510 1037 1008 127 66
MspA1I CmG'CkG
Cuts at: 1859 2173 2533 3546 3791 1859
Size: 314 360 1013 245 2816
Fragments arranged by size:
2816 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2929 2929
Size: 4748
NarI GG'CG_CC
Cuts at: 2179 2427 2179
Size: 248 4500
NdeI CA'TA_TG
Cuts at: 623 623
Size: 4748
NgoMIV G'CCGG_C
Cuts at: 2927 2927
Size: 4748
NheI G'CTAG_C
Cuts at: 784 784
Size: 4748
NspI r_CATG'y
Cuts at: 145 894 1495 1550 1801 2301 3208 145
Size: 749 601 55 251 500 907 1685
Fragments arranged by size:
1685 907 749 601 500 251 55
PciI A'CATG_T
Cuts at: 890 3204 890
Size: 2314 2434
PmeI GTTT'AAAC
Cuts at: 1747 1747
Size: 4748
PmlI CAC'GTG
Cuts at: 559 1278 1721 559
Size: 719 443 3586
Fragments arranged by size:
3586 719 443
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3887 3919 3887
Size: 32 4716
PpuMI rG'GwC_Cy
Cuts at: 415 736 1257 415
Size: 321 521 3906
Fragments arranged by size:
3906 521 321
PspOMI G'GGCC_C
Cuts at: 1134 1134
Size: 4748
PstI C_TGCA'G
Cuts at: 639 1338 1795 2480 639
Size: 699 457 685 2907
Fragments arranged by size:
2907 699 685 457
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4748
PvuII CAG'CTG
Cuts at: 2173 2533 2173
Size: 360 4388
RsrII CG'GwC_CG
Cuts at: 2943 2943
Size: 4748
SacI G_AGCT'C
Cuts at: 1762 1762
Size: 4748
SalI G'TCGA_C
Cuts at: 1785 1785
Size: 4748
SapI GCTCTTCn'nnn_
Cuts at: 2771 2981 2771
Size: 210 4538
SbfI CC_TGCA'GG
Cuts at: 1795 1795
Size: 4748
ScaI AGT'ACT
Cuts at: 4265 4265
Size: 4748
SfcI C'TryA_G
Cuts at: 33 635 1250 1334 1689 1791 2476 3469 3660
Size: 602 615 84 355 102 685 993 191
Cuts at: 3660 33
Size: 1121
Fragments arranged by size:
1121 993 685 615 602 355 191 102
84
SfoI GGC'GCC
Cuts at: 2180 2428 2180
Size: 248 4500
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4748
SmaI CCC'GGG
Cuts at: 1770 1770
Size: 4748
SmlI C'TyrA_G
Cuts at: 1903 2818 3310 3572 1903
Size: 915 492 262 3079
Fragments arranged by size:
3079 915 492 262
SnaBI TAC'GTA
Cuts at: 1740 1740
Size: 4748
SpeI A'CTAG_T
Cuts at: 67 1009 67
Size: 942 3806
SphI G_CATG'C
Cuts at: 145 1801 2301 145
Size: 1656 500 2592
Fragments arranged by size:
2592 1656 500
SspI AAT'ATT
Cuts at: 883 883
Size: 4748
StyI C'CwwG_G
Cuts at: 853 1882 2236 853
Size: 1029 354 3365
Fragments arranged by size:
3365 1029 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 208 1228 2331 2464 2923 3132 208
Size: 1020 1103 133 459 209 1824
Fragments arranged by size:
1824 1103 1020 459 209 133
TatI w'GTAC_w
Cuts at: 4263 4263
Size: 4748
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1011 1043 1074 1106 1308 1340 4265 4297 1011
Size: 32 31 32 202 32 2925 32 1462
Fragments arranged by size:
2925 1462 202 32 32 32 32 31
Tth111I GACn'n_nGTC
Cuts at: 2545 2545
Size: 4748
XbaI T'CTAG_A
Cuts at: 1779 1779
Size: 4748
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2243 2243
Size: 4748
XmaI C'CCGG_G
Cuts at: 1768 1768
Size: 4748
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NheI NspI PciI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BpmI Bpu10I
BseRI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
BtgI ClaI CspCI DraIII FseI FspAI HpaI MfeI
NcoI NotI NruI NsiI PacI PasI PflMI PfoI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SrfI StuI SwaI XhoI XmnI ZraI