Restriction map for
pF1KSDA1267
(Circular) (Six-base) MAPSORT of: pF1KSDA1267.seq Check: 1601 from: 1 to: 6452
pF1KSDA1267 6452 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 1729 3490 1729
Size: 1761 4691
Acc65I G'GTAC_C
Cuts at: 1578 3468 1578
Size: 1890 4562
AclI AA'CG_TT
Cuts at: 6292 6292
Size: 6452
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 185 414 1196 1199 1465 1765 2794 2839 4277
Size: 229 782 3 266 300 1029 45 1438
Cuts at: 4277 4709 5456 5724 185
Size: 432 747 268 913
Fragments arranged by size:
1438 1029 913 782 747 432 300 268
266 229 45 3
AfeI AGC'GCT
Cuts at: 1036 2434 1036
Size: 1398 5054
AflII C'TTAA_G
Cuts at: 2239 2239
Size: 6452
AflIII A'CryG_T
Cuts at: 1903 3804 4908 5885 1903
Size: 1901 1104 977 2470
Fragments arranged by size:
2470 1901 1104 977
AgeI A'CCGG_T
Cuts at: 4840 4840
Size: 6452
AleI CACnn'nnGTG
Cuts at: 1888 2935 3136 1888
Size: 1047 201 5204
Fragments arranged by size:
5204 1047 201
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3327 3327
Size: 6452
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3203 3235 3203
Size: 32 6420
AlwNI CAG_nnn'CTG
Cuts at: 163 1469 2817 3757 5324 163
Size: 1306 1348 940 1567 1291
Fragments arranged by size:
1567 1348 1306 1291 940
ApaLI G'TGCA_C
Cuts at: 5222 5222
Size: 6452
ApoI r'AATT_y
Cuts at: 1511 2066 3456 5805 6061 1511
Size: 555 1390 2349 256 1902
Fragments arranged by size:
2349 1902 1390 555 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6452
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6452
AvaI C'yCGr_G
Cuts at: 1393 3143 3472 1393
Size: 1750 329 4373
Fragments arranged by size:
4373 1750 329
AvrII C'CTAG_G
Cuts at: 3091 3091
Size: 6452
BamHI G'GATC_C
Cuts at: 297 3477 297
Size: 3180 3272
BanI G'GyrC_C
Cuts at: 138 1578 1693 1882 3468 3882 4130 4165 138
Size: 1440 115 189 1586 414 248 35 2425
Fragments arranged by size:
2425 1586 1440 414 248 189 115 35
BanII G_rGCy'C
Cuts at: 672 2701 3100 3466 4496 672
Size: 2029 399 366 1030 2628
Fragments arranged by size:
2628 2029 1030 399 366
BbeI G_GCGC'C
Cuts at: 142 3886 4134 142
Size: 3744 248 2460
Fragments arranged by size:
3744 2460 248
BbsI GAAGACnn'nnnn_
Cuts at: 341 420 929 1111 1639 341
Size: 79 509 182 528 5154
Fragments arranged by size:
5154 528 509 182 79
BbvCI CC'TCA_GC
Cuts at: 1826 1826
Size: 6452
BciVI GTATCCnnnnn_n'
Cuts at: 3168 4350 5111 6452 3168
Size: 1182 761 1341 3168
Fragments arranged by size:
3168 1341 1182 761
BclI T'GATC_A
Cuts at: 630 3972 630
Size: 3342 3110
BglI GCCn_nnn'nGGC
Cuts at: 2793 2793
Size: 6452
BglII A'GATC_T
Cuts at: 3967 6004 3967
Size: 2037 4415
BlpI GC'TnA_GC
Cuts at: 491 3422 3564 491
Size: 2931 142 3379
Fragments arranged by size:
3379 2931 142
Bme1580I G_kGCm'C
Cuts at: 1020 1698 2399 2916 4077 4170 5226 1020
Size: 678 701 517 1161 93 1056 2246
Fragments arranged by size:
2246 1161 1056 701 678 517 93
BmgBI CAC'GTC
Cuts at: 1906 1906
Size: 6452
BmrI ACTGGGnnnn_n'
Cuts at: 2097 3664 3745 4080 6224 2097
Size: 1567 81 335 2144 2325
Fragments arranged by size:
2325 2144 1567 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4819 4819
Size: 6452
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 870 870
Size: 6452
Bpu10I CC'TnA_GC
Cuts at: 1826 1826
Size: 6452
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1158 1206 1317 3373 3628 4507 4999 5297 1158
Size: 48 111 2056 255 879 492 298 2313
Fragments arranged by size:
2313 2056 879 492 298 255 111 48
BsaI GGTCTCn'nnnn_
Cuts at: 1134 1198 2486 6156 1134
Size: 64 1288 3670 1430
Fragments arranged by size:
3670 1430 1288 64
BsaAI yAC'GTr
Cuts at: 1546 3444 3805 1546
Size: 1898 361 4193
Fragments arranged by size:
4193 1898 361
BsaBI GATnn'nnATC
Cuts at: 6 714 2012 3966 6003 6
Size: 708 1298 1954 2037 455
Fragments arranged by size:
2037 1954 1298 708 455
BsaHI Gr'CG_yC
Cuts at: 139 3883 4131 4833 6329 139
Size: 3744 248 702 1496 262
Fragments arranged by size:
3744 1496 702 262 248
BsaWI w'CCGG_w
Cuts at: 179 1979 3641 4162 4840 5114 5261 5672 179
Size: 1800 1662 521 678 274 147 411 959
Fragments arranged by size:
1800 1662 959 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2043 2073 3357 3387 2043
Size: 30 1284 30 5108
Fragments arranged by size:
5108 1284 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2601 2697 2851 2914 2601
Size: 96 154 63 6139
Fragments arranged by size:
6139 154 96 63
BseYI C'CCAG_C
Cuts at: 301 1086 2788 2945 2996 3000 3026 3198 3877
Size: 785 1702 157 51 4 26 172 679
Cuts at: 3877 4635 5212 5708 301
Size: 758 577 496 1045
Fragments arranged by size:
1702 1045 785 758 679 577 496 172
157 51 26 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1323 2609 3100 3297 1323
Size: 1286 491 197 4478
Fragments arranged by size:
4478 1286 491 197
BsiEI CG_ry'CG
Cuts at: 104 2985 3994 4040 5248 104
Size: 2881 1009 46 1208 1308
Fragments arranged by size:
2881 1308 1208 1009 46
BsiHKAI G_wGCw'C
Cuts at: 1749 2253 2701 3466 4244 4434 5226 1749
Size: 504 448 765 778 190 792 2975
Fragments arranged by size:
2975 792 778 765 504 448 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1410 1869 1410
Size: 459 5993
Bsp1286I G_dGCh'C
Cuts at: 672 1020 1698 1749 2253 2399 2701 2916 3100
Size: 348 678 51 504 146 302 215 184
Cuts at: 3100 3466 4077 4170 4244 4434 4496 5226 672
Size: 366 611 93 74 190 62 730 1898
Fragments arranged by size:
1898 730 678 611 504 366 348 302
215 190 184 146 93 74 62 51
BspEI T'CCGG_A
Cuts at: 5672 5672
Size: 6452
BspHI T'CATG_A
Cuts at: 633 5628 633
Size: 4995 1457
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1050 1412 2951 3502 3716 4018 4399 1050
Size: 362 1539 551 214 302 381 3103
Fragments arranged by size:
3103 1539 551 381 362 302 214
BsrBI CCG'CTC
Cuts at: 50 145 3234 4744 4798 6282 6447 50
Size: 95 3089 1510 54 1484 165 55
Fragments arranged by size:
3089 1510 1484 165 95 55 54
BsrDI GCAATG_nn'
Cuts at: 284 4364 284
Size: 4080 2372
BsrFI r'CCGG_y
Cuts at: 843 4450 4631 4840 843
Size: 3607 181 209 2455
Fragments arranged by size:
3607 2455 209 181
BssSI C'ACGA_G
Cuts at: 2931 4723 5081 2931
Size: 1792 358 4302
Fragments arranged by size:
4302 1792 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3829 3829
Size: 6452
BstBI TT'CG_AA
Cuts at: 110 4813 110
Size: 4703 1749
BstYI r'GATC_y
Cuts at: 1 297 709 3477 3944 3967 4302 4548 5560
Size: 296 412 2768 467 23 335 246 1012
Cuts at: 5560 5646 5658 6004 1
Size: 86 12 346 449
Fragments arranged by size:
2768 1012 467 449 412 346 335 296
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3327 3327
Size: 6452
BtgI C'CryG_G
Cuts at: 125 833 1144 1689 2399 3078 125
Size: 708 311 545 710 679 3499
Fragments arranged by size:
3499 710 708 679 545 311
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 148 2648 4402 4583 4746 148
Size: 2500 1754 181 163 1854
Fragments arranged by size:
2500 1854 1754 181 163
BtsI GCAGTG_nn'
Cuts at: 228 806 1311 3012 3695 228
Size: 578 505 1701 683 2985
Fragments arranged by size:
2985 1701 683 578 505
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1722 1757 1722
Size: 35 6417
DraI TTT'AAA
Cuts at: 3451 3451
Size: 6452
DraIII CAC_nnn'GTG
Cuts at: 2032 2032
Size: 6452
DrdI GACnn_nn'nnGTC
Cuts at: 4158 5016 4158
Size: 858 5594
EaeI y'GGCC_r
Cuts at: 2982 3342 4037 4211 4602 4629 5974 2982
Size: 360 695 174 391 27 1345 3460
Fragments arranged by size:
3460 1345 695 391 360 174 27
EagI C'GGCC_G
Cuts at: 2982 4037 2982
Size: 1055 5397
EarI CTCTTCn'nnn_
Cuts at: 692 758 1361 1784 1872 2840 4475 4685 692
Size: 66 603 423 88 968 1635 210 2459
Fragments arranged by size:
2459 1635 968 603 423 210 88 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1984 3252 3283 4660 4970 5116 6262 1984
Size: 1268 31 1377 310 146 1146 2174
Fragments arranged by size:
2174 1377 1268 1146 310 146 31
EcoICRI GAG'CTC
Cuts at: 2699 3464 2699
Size: 765 5687
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 185 414 870 1196 1199 1465 1765 2794 2839
Size: 229 456 326 3 266 300 1029 45
Cuts at: 2839 4277 4709 5456 5724 185
Size: 1438 432 747 268 913
Fragments arranged by size:
1438 1029 913 747 456 432 326 300
268 266 229 45 3
EcoO109I rG'GnC_Cy
Cuts at: 2360 2856 2963 3591 2360
Size: 496 107 628 5221
Fragments arranged by size:
5221 628 496 107
EcoRI G'AATT_C
Cuts at: 1511 3456 1511
Size: 1945 4507
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 354 4471 354
Size: 4117 2335
FspI TGC'GCA
Cuts at: 2025 4233 2025
Size: 2208 4244
HaeII r_GCGC'y
Cuts at: 142 1038 2422 2436 3364 3429 3886 4134 5156
Size: 896 1384 14 928 65 457 248 1022
Cuts at: 5156 6135 142
Size: 979 459
Fragments arranged by size:
1384 1022 979 928 896 459 457 248
65 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 373 405 874 906 1149 1181 1711 1743 1844
Size: 32 469 32 243 32 530 32 101
Cuts at: 1844 1876 2485 2517 2797 2829 2845 2877 3199
Size: 32 609 32 280 32 16 32 322
Cuts at: 3199 3231 3384 3416 4294 4324 4326 4356 373
Size: 32 153 32 878 30 2 30 2469
Fragments arranged by size:
2469 878 609 530 469 322 280 243
153 101 32 32 32 32 32 32
32 32 32 32 30 30 16 2
HincII GTy'rAC
Cuts at: 3491 3491
Size: 6452
KasI G'GCGC_C
Cuts at: 138 3882 4130 138
Size: 3744 248 2460
Fragments arranged by size:
3744 2460 248
KpnI G_GTAC'C
Cuts at: 1582 3472 1582
Size: 1890 4562
MfeI C'AATT_G
Cuts at: 744 744
Size: 6452
MluI A'CGCG_T
Cuts at: 5885 5885
Size: 6452
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2069 2087 2888 5123 5307 2069
Size: 18 801 2235 184 3214
Fragments arranged by size:
3214 2235 801 184 18
MscI TGG'CCA
Cuts at: 4213 4213
Size: 6452
MslI CAynn'nnrTG
Cuts at: 695 1888 2536 2728 2935 3136 4441 4568 695
Size: 1193 648 192 207 201 1305 127 2579
Fragments arranged by size:
2579 1305 1193 648 207 201 192 127
MspA1I CmG'CkG
Cuts at: 163 1086 1469 2792 2949 3000 3285 3311 3563
Size: 923 383 1323 157 51 285 26 252
Cuts at: 3563 3877 4237 5250 5495 163
Size: 314 360 1013 245 1120
Fragments arranged by size:
1323 1120 1013 923 383 360 314 285
252 245 157 51 26
NaeI GCC'GGC
Cuts at: 845 4633 845
Size: 3788 2664
NarI GG'CG_CC
Cuts at: 139 3883 4131 139
Size: 3744 248 2460
Fragments arranged by size:
3744 2460 248
NcoI C'CATG_G
Cuts at: 125 833 1689 2399 3078 125
Size: 708 856 710 679 3499
Fragments arranged by size:
3499 856 710 708 679
NgoMIV G'CCGG_C
Cuts at: 843 4631 843
Size: 3788 2664
NspI r_CATG'y
Cuts at: 2178 2890 3505 4005 4912 2178
Size: 712 615 500 907 3718
Fragments arranged by size:
3718 907 712 615 500
PasI CC'CwG_GG
Cuts at: 3227 3227
Size: 6452
PciI A'CATG_T
Cuts at: 4908 4908
Size: 6452
PmeI GTTT'AAAC
Cuts at: 3451 3451
Size: 6452
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5591 5623 5591
Size: 32 6420
PpuMI rG'GwC_Cy
Cuts at: 2360 2856 2963 2360
Size: 496 107 5849
Fragments arranged by size:
5849 496 107
PshAI GACnn'nnGTC
Cuts at: 1422 1422
Size: 6452
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 377 409 534 566 377
Size: 32 125 32 6263
Fragments arranged by size:
6263 125 32 32
PstI C_TGCA'G
Cuts at: 294 1446 3499 4184 294
Size: 1152 2053 685 2562
Fragments arranged by size:
2562 2053 1152 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6452
PvuII CAG'CTG
Cuts at: 163 1086 2792 3000 3285 3877 4237 163
Size: 923 1706 208 285 592 360 2378
Fragments arranged by size:
2378 1706 923 592 360 285 208
RsrII CG'GwC_CG
Cuts at: 4647 4647
Size: 6452
SacI G_AGCT'C
Cuts at: 2701 3466 2701
Size: 765 5687
SalI G'TCGA_C
Cuts at: 3489 3489
Size: 6452
SapI GCTCTTCn'nnn_
Cuts at: 692 4475 4685 692
Size: 3783 210 2459
Fragments arranged by size:
3783 2459 210
SbfI CC_TGCA'GG
Cuts at: 3499 3499
Size: 6452
ScaI AGT'ACT
Cuts at: 5969 5969
Size: 6452
SexAI A'CCwGG_T
Cuts at: 873 873
Size: 6452
SfcI C'TryA_G
Cuts at: 33 290 1442 2967 3495 4180 5173 5364 33
Size: 257 1152 1525 528 685 993 191 1121
Fragments arranged by size:
1525 1152 1121 993 685 528 257 191
SfoI GGC'GCC
Cuts at: 140 3884 4132 140
Size: 3744 248 2460
Fragments arranged by size:
3744 2460 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6452
SmaI CCC'GGG
Cuts at: 3474 3474
Size: 6452
SmlI C'TyrA_G
Cuts at: 1137 1185 1296 2239 3388 3607 4522 5014 5276
Size: 48 111 943 1149 219 915 492 262
Cuts at: 5276 1137
Size: 2313
Fragments arranged by size:
2313 1149 943 915 492 262 219 111
48
SnaBI TAC'GTA
Cuts at: 3444 3444
Size: 6452
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6452
SphI G_CATG'C
Cuts at: 2178 2890 3505 4005 2178
Size: 712 615 500 4625
Fragments arranged by size:
4625 712 615 500
StyI C'CwwG_G
Cuts at: 125 329 833 1689 2399 2588 3078 3091 3586
Size: 204 504 856 710 189 490 13 495
Cuts at: 3586 3940 125
Size: 354 2637
Fragments arranged by size:
2637 856 710 504 495 490 354 204
189 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1078 2268 2581 4035 4168 4627 4836 1078
Size: 1190 313 1454 133 459 209 2694
Fragments arranged by size:
2694 1454 1190 459 313 209 133
TatI w'GTAC_w
Cuts at: 1911 5967 1911
Size: 4056 2396
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 161 193 2043 2075 2556 2588 2919 2951 3357
Size: 32 1850 32 481 32 331 32 406
Cuts at: 3357 3389 5969 6001 161
Size: 32 2580 32 612
Fragments arranged by size:
2580 1850 612 481 406 331 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4249 4249
Size: 6452
XbaI T'CTAG_A
Cuts at: 3483 3483
Size: 6452
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 336 894 3195 3947 132
Size: 204 558 2301 752 2637
Fragments arranged by size:
2637 2301 752 558 204
XmaI C'CCGG_G
Cuts at: 3472 3472
Size: 6452
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PmeI
PpiI PpuMI PshAI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AhdI ApaI AscI BaeI BcgI BfrBI
BmtI BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I
ClaI EcoNI EcoRV FseI FspAI HindIII HpaI NdeI
NheI NotI NruI NsiI PacI PflMI PfoI PmlI
PsiI PspOMI PspXI SacII SanDI SfiI SgrAI SrfI
SspI StuI SwaI XhoI XmnI ZraI