Restriction map for
pF1KSDA1269
(Circular) (Six-base) MAPSORT of: pF1KSDA1269.seq Check: 727 from: 1 to: 4727
pF1KSDA1269 4727 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 655 1236 655
Size: 581 4146
AccI GT'mk_AC
Cuts at: 1765 1765
Size: 4727
Acc65I G'GTAC_C
Cuts at: 1743 1743
Size: 4727
AclI AA'CG_TT
Cuts at: 4567 4567
Size: 4727
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 562 865 1144 1354 2552 2984 3731 3999
Size: 176 303 279 210 1198 432 747 268
Cuts at: 3999 386
Size: 1114
Fragments arranged by size:
1198 1114 747 432 303 279 268 210
176
AflIII A'CryG_T
Cuts at: 1475 2079 3183 4160 1475
Size: 604 1104 977 2042
Fragments arranged by size:
2042 1104 977 604
AgeI A'CCGG_T
Cuts at: 3115 3115
Size: 4727
AleI CACnn'nnGTG
Cuts at: 190 1624 190
Size: 1434 3293
AlwNI CAG_nnn'CTG
Cuts at: 894 1645 2032 3599 894
Size: 751 387 1567 2022
Fragments arranged by size:
2022 1567 751 387
ApaI G_GGCC'C
Cuts at: 1579 1579
Size: 4727
ApaLI G'TGCA_C
Cuts at: 148 517 3497 148
Size: 369 2980 1378
Fragments arranged by size:
2980 1378 369
ApoI r'AATT_y
Cuts at: 511 1731 4080 4336 511
Size: 1220 2349 256 902
Fragments arranged by size:
2349 1220 902 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4727
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4727
AvaI C'yCGr_G
Cuts at: 1064 1281 1408 1651 1747 1064
Size: 217 127 243 96 4044
Fragments arranged by size:
4044 243 217 127 96
BamHI G'GATC_C
Cuts at: 1752 1752
Size: 4727
BanI G'GyrC_C
Cuts at: 1374 1743 2157 2405 2440 1374
Size: 369 414 248 35 3661
Fragments arranged by size:
3661 414 369 248 35
BanII G_rGCy'C
Cuts at: 681 1579 1741 2771 681
Size: 898 162 1030 2637
Fragments arranged by size:
2637 1030 898 162
BbeI G_GCGC'C
Cuts at: 1378 2161 2409 1378
Size: 783 248 3696
Fragments arranged by size:
3696 783 248
BbsI GAAGACnn'nnnn_
Cuts at: 226 791 226
Size: 565 4162
BbvCI CC'TCA_GC
Cuts at: 1144 1144
Size: 4727
BciVI GTATCCnnnnn_n'
Cuts at: 1408 2625 3386 4727 1408
Size: 1217 761 1341 1408
Fragments arranged by size:
1408 1341 1217 761
BclI T'GATC_A
Cuts at: 2247 2247
Size: 4727
BglI GCCn_nnn'nGGC
Cuts at: 1237 1270 1237
Size: 33 4694
BglII A'GATC_T
Cuts at: 2242 4279 2242
Size: 2037 2690
BlpI GC'TnA_GC
Cuts at: 1839 1839
Size: 4727
Bme1580I G_kGCm'C
Cuts at: 152 521 1292 1316 1579 1637 2352 2445 3501
Size: 369 771 24 263 58 715 93 1056
Cuts at: 3501 152
Size: 1378
Fragments arranged by size:
1378 1056 771 715 369 263 93 58
24
BmgBI CAC'GTC
Cuts at: 141 704 744 141
Size: 563 40 4124
Fragments arranged by size:
4124 563 40
BmrI ACTGGGnnnn_n'
Cuts at: 1251 1261 1939 2020 2355 4499 1251
Size: 10 678 81 335 2144 1479
Fragments arranged by size:
2144 1479 678 335 81 10
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 448 3094 448
Size: 2646 2081
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 459 1102 1599 459
Size: 643 497 3587
Fragments arranged by size:
3587 643 497
Bpu10I CC'TnA_GC
Cuts at: 583 691 1144 1495 583
Size: 108 453 351 3815
Fragments arranged by size:
3815 453 351 108
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 1903 2782 3274 3572 322
Size: 1581 879 492 298 1477
Fragments arranged by size:
1581 1477 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 758 769 895 1179 4431 758
Size: 11 126 284 3252 1054
Fragments arranged by size:
3252 1054 284 126 11
BsaAI yAC'GTr
Cuts at: 1213 1719 2080 1213
Size: 506 361 3860
Fragments arranged by size:
3860 506 361
BsaBI GATnn'nnATC
Cuts at: 6 1100 2241 4278 6
Size: 1094 1141 2037 455
Fragments arranged by size:
2037 1141 1094 455
BsaHI Gr'CG_yC
Cuts at: 851 1168 1375 2158 2406 3108 4604 851
Size: 317 207 783 248 702 1496 974
Fragments arranged by size:
1496 974 783 702 317 248 207
BsaWI w'CCGG_w
Cuts at: 180 1916 2437 3115 3389 3536 3947 180
Size: 1736 521 678 274 147 411 960
Fragments arranged by size:
1736 960 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 668 698 1075 1105 668
Size: 30 377 30 4290
Fragments arranged by size:
4290 377 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 172 318 1675 172
Size: 146 1357 3224
Fragments arranged by size:
3224 1357 146
BseYI C'CCAG_C
Cuts at: 332 1136 1298 1450 2152 2910 3487 3983 332
Size: 804 162 152 702 758 577 496 1076
Fragments arranged by size:
1076 804 758 702 577 496 162 152
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1000 1531 1000
Size: 531 4196
BsiEI CG_ry'CG
Cuts at: 104 710 1232 1505 2269 2315 3523 104
Size: 606 522 273 764 46 1208 1308
Fragments arranged by size:
1308 1208 764 606 522 273 46
BsiHKAI G_wGCw'C
Cuts at: 152 521 1157 1253 1501 1667 1741 2519 2709
Size: 369 636 96 248 166 74 778 190
Cuts at: 2709 3501 152
Size: 792 1378
Fragments arranged by size:
1378 792 778 636 369 248 190 166
96 74
Bsp1286I G_dGCh'C
Cuts at: 152 521 681 1157 1253 1292 1316 1501 1579
Size: 369 160 476 96 39 24 185 78
Cuts at: 1579 1637 1667 1741 2352 2445 2519 2709 2771
Size: 58 30 74 611 93 74 190 62
Cuts at: 2771 3501 152
Size: 730 1378
Fragments arranged by size:
1378 730 611 476 369 190 185 160
96 93 78 74 74 62 58 39
30 24
BspEI T'CCGG_A
Cuts at: 180 3947 180
Size: 3767 960
BspHI T'CATG_A
Cuts at: 1157 3903 1157
Size: 2746 1981
BspMI ACCTGCnnnn'nnnn_
Cuts at: 655 1236 1777 1991 2293 2674 655
Size: 581 541 214 302 381 2708
Fragments arranged by size:
2708 581 541 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1046 1710 3019 3073 4557 4722 50
Size: 996 664 1309 54 1484 165 55
Fragments arranged by size:
1484 1309 996 664 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2639 2639
Size: 4727
BsrFI r'CCGG_y
Cuts at: 853 1170 1227 2725 2906 3115 853
Size: 317 57 1498 181 209 2465
Fragments arranged by size:
2465 1498 317 209 181 57
BssSI C'ACGA_G
Cuts at: 1705 2998 3356 1705
Size: 1293 358 3076
Fragments arranged by size:
3076 1293 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1645 2104 1645
Size: 459 4268
BstBI TT'CG_AA
Cuts at: 110 3088 110
Size: 2978 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1353 1458 1683 1353
Size: 105 225 4397
Fragments arranged by size:
4397 225 105
BstYI r'GATC_y
Cuts at: 1 1463 1752 2219 2242 2577 2823 3835 3921
Size: 1462 289 467 23 335 246 1012 86
Cuts at: 3921 3933 4279 1
Size: 12 346 449
Fragments arranged by size:
1462 1012 467 449 346 335 289 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1658 1658
Size: 4727
BtgI C'CryG_G
Cuts at: 125 311 803 1235 1691 125
Size: 186 492 432 456 3161
Fragments arranged by size:
3161 492 456 432 186
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2677 2858 3021 2677
Size: 181 163 4383
Fragments arranged by size:
4383 181 163
BtsI GCAGTG_nn'
Cuts at: 185 197 207 1096 1970 185
Size: 12 10 889 874 2942
Fragments arranged by size:
2942 889 874 12 10
DraI TTT'AAA
Cuts at: 1726 1726
Size: 4727
DrdI GACnn_nn'nnGTC
Cuts at: 2433 3291 2433
Size: 858 3869
EaeI y'GGCC_r
Cuts at: 524 707 806 1019 1229 1502 1670 2312 2486
Size: 183 99 213 210 273 168 642 174
Cuts at: 2486 2877 2904 4249 524
Size: 391 27 1345 1002
Fragments arranged by size:
1345 1002 642 391 273 213 210 183
174 168 99 27
EagI C'GGCC_G
Cuts at: 707 1229 1502 2312 707
Size: 522 273 810 3122
Fragments arranged by size:
3122 810 522 273
EarI CTCTTCn'nnn_
Cuts at: 362 547 1437 2750 2960 362
Size: 185 890 1313 210 2129
Fragments arranged by size:
2129 1313 890 210 185
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2935 3245 3391 4537 2935
Size: 310 146 1146 3125
Fragments arranged by size:
3125 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1739 1739
Size: 4727
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 459 562 865 1102 1144 1354 1599 2552
Size: 73 103 303 237 42 210 245 953
Cuts at: 2552 2984 3731 3999 386
Size: 432 747 268 1114
Fragments arranged by size:
1114 953 747 432 303 268 245 237
210 103 73 42
EcoNI CCTnn'n_nnAGG
Cuts at: 1357 1357
Size: 4727
EcoO109I rG'GnC_Cy
Cuts at: 933 1068 1576 1866 933
Size: 135 508 290 3794
Fragments arranged by size:
3794 508 290 135
EcoRI G'AATT_C
Cuts at: 1731 1731
Size: 4727
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2746 2746
Size: 4727
FspI TGC'GCA
Cuts at: 2508 2508
Size: 4727
HaeII r_GCGC'y
Cuts at: 1276 1378 2161 2409 3431 4410 1276
Size: 102 783 248 1022 979 1593
Fragments arranged by size:
1593 1022 979 783 248 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1406 1438 2569 2599 2601 2631 1406
Size: 32 1131 30 2 30 3502
Fragments arranged by size:
3502 1131 32 30 30 2
HincII GTy'rAC
Cuts at: 1766 1766
Size: 4727
KasI G'GCGC_C
Cuts at: 1374 2157 2405 1374
Size: 783 248 3696
Fragments arranged by size:
3696 783 248
KpnI G_GTAC'C
Cuts at: 1747 1747
Size: 4727
MfeI C'AATT_G
Cuts at: 1622 1622
Size: 4727
MluI A'CGCG_T
Cuts at: 1475 4160 1475
Size: 2685 2042
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 665 3398 3582 665
Size: 2733 184 1810
Fragments arranged by size:
2733 1810 184
MscI TGG'CCA
Cuts at: 1021 2488 1021
Size: 1467 3260
MslI CAynn'nnrTG
Cuts at: 190 466 565 1624 1681 2716 2843 190
Size: 276 99 1059 57 1035 127 2074
Fragments arranged by size:
2074 1059 1035 276 127 99 57
MspA1I CmG'CkG
Cuts at: 298 307 313 805 924 1302 1404 1548 1554
Size: 9 6 492 119 378 102 144 6
Cuts at: 1554 1838 2152 2512 3525 3770 298
Size: 284 314 360 1013 245 1255
Fragments arranged by size:
1255 1013 492 378 360 314 284 245
144 119 102 9 6 6
NaeI GCC'GGC
Cuts at: 855 2908 855
Size: 2053 2674
NarI GG'CG_CC
Cuts at: 1375 2158 2406 1375
Size: 783 248 3696
Fragments arranged by size:
3696 783 248
NcoI C'CATG_G
Cuts at: 125 1235 1691 125
Size: 1110 456 3161
Fragments arranged by size:
3161 1110 456
NgoMIV G'CCGG_C
Cuts at: 853 2906 853
Size: 2053 2674
NspI r_CATG'y
Cuts at: 496 996 1780 2280 3187 496
Size: 500 784 500 907 2036
Fragments arranged by size:
2036 907 784 500 500
PciI A'CATG_T
Cuts at: 3183 3183
Size: 4727
PflMI CCAn_nnn'nTGG
Cuts at: 894 894
Size: 4727
PfoI T'CCnGG_A
Cuts at: 875 929 875
Size: 54 4673
PmeI GTTT'AAAC
Cuts at: 1726 1726
Size: 4727
PmlI CAC'GTG
Cuts at: 1213 1213
Size: 4727
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3866 3898 3866
Size: 32 4695
PpuMI rG'GwC_Cy
Cuts at: 933 1068 933
Size: 135 4592
PshAI GACnn'nnGTC
Cuts at: 1171 1171
Size: 4727
PspOMI G'GGCC_C
Cuts at: 1575 1575
Size: 4727
PstI C_TGCA'G
Cuts at: 192 920 947 1553 1589 1774 2459 192
Size: 728 27 606 36 185 685 2460
Fragments arranged by size:
2460 728 685 606 185 36 27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4727
PvuII CAG'CTG
Cuts at: 307 1302 1554 2152 2512 307
Size: 995 252 598 360 2522
Fragments arranged by size:
2522 995 598 360 252
RsrII CG'GwC_CG
Cuts at: 2922 2922
Size: 4727
SacI G_AGCT'C
Cuts at: 1741 1741
Size: 4727
SacII CC_GC'GG
Cuts at: 314 806 314
Size: 492 4235
SalI G'TCGA_C
Cuts at: 1764 1764
Size: 4727
SapI GCTCTTCn'nnn_
Cuts at: 2750 2960 2750
Size: 210 4517
SbfI CC_TGCA'GG
Cuts at: 1774 1774
Size: 4727
ScaI AGT'ACT
Cuts at: 4244 4244
Size: 4727
SexAI A'CCwGG_T
Cuts at: 1001 1001
Size: 4727
SfcI C'TryA_G
Cuts at: 33 188 916 943 1549 1585 1770 2455 3448
Size: 155 728 27 606 36 185 685 993
Cuts at: 3448 3639 33
Size: 191 1121
Fragments arranged by size:
1121 993 728 685 606 191 185 155
36 27
SfiI GGCCn_nnn'nGGCC
Cuts at: 1237 1237
Size: 4727
SfoI GGC'GCC
Cuts at: 1376 2159 2407 1376
Size: 783 248 3696
Fragments arranged by size:
3696 783 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4727
SmaI CCC'GGG
Cuts at: 1066 1283 1653 1749 1066
Size: 217 370 96 4044
Fragments arranged by size:
4044 370 217 96
SmlI C'TyrA_G
Cuts at: 337 1882 2797 3289 3551 337
Size: 1545 915 492 262 1513
Fragments arranged by size:
1545 1513 915 492 262
SnaBI TAC'GTA
Cuts at: 1719 1719
Size: 4727
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4727
SphI G_CATG'C
Cuts at: 996 1780 2280 996
Size: 784 500 3443
Fragments arranged by size:
3443 784 500
SrfI GCCC'GGGC
Cuts at: 1283 1283
Size: 4727
StuI AGG'CCT
Cuts at: 914 1603 914
Size: 689 4038
StyI C'CwwG_G
Cuts at: 125 1115 1235 1317 1691 1861 2215 125
Size: 990 120 82 374 170 354 2637
Fragments arranged by size:
2637 990 374 354 170 120 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1270 2310 2443 2902 3111 1270
Size: 1040 133 459 209 2886
Fragments arranged by size:
2886 1040 459 209 133
TatI w'GTAC_w
Cuts at: 713 960 4242 713
Size: 247 3282 1198
Fragments arranged by size:
3282 1198 247
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 668 700 1075 1107 4244 4276 668
Size: 32 375 32 3137 32 1119
Fragments arranged by size:
3137 1119 375 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 631 868 2524 631
Size: 237 1656 2834
Fragments arranged by size:
2834 1656 237
XbaI T'CTAG_A
Cuts at: 1758 1758
Size: 4727
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1606 1680 2222 1606
Size: 74 542 4111
Fragments arranged by size:
4111 542 74
XmaI C'CCGG_G
Cuts at: 1064 1281 1651 1747 1064
Size: 217 370 96 4044
Fragments arranged by size:
4044 370 217 96
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BcgI BfrBI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI NdeI NheI NotI NruI NsiI
PacI PasI PsiI PspXI PsrI SanDI SgrAI SspI
SwaI XhoI XmnI ZraI