Restriction map for
pF1KSDA1284
(Circular) (Six-base) MAPSORT of: pF1KSDA1284.seq Check: 9729 from: 1 to: 5963
pF1KSDA1284 5963 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 882 882
Size: 5963
AccI GT'mk_AC
Cuts at: 1660 1745 2413 3001 1660
Size: 85 668 588 4622
Fragments arranged by size:
4622 668 588 85
Acc65I G'GTAC_C
Cuts at: 2979 2979
Size: 5963
AclI AA'CG_TT
Cuts at: 5803 5803
Size: 5963
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1148 1322 1438 1883 1901 3788 4220 4967 5235
Size: 174 116 445 18 1887 432 747 268
Cuts at: 5235 1148
Size: 1876
Fragments arranged by size:
1887 1876 747 445 432 268 174 116
18
AfeI AGC'GCT
Cuts at: 505 505
Size: 5963
AflII C'TTAA_G
Cuts at: 2392 2392
Size: 5963
AflIII A'CryG_T
Cuts at: 3315 4419 5396 3315
Size: 1104 977 3882
Fragments arranged by size:
3882 1104 977
AgeI A'CCGG_T
Cuts at: 4351 4351
Size: 5963
AhdI GACnn_n'nnGTC
Cuts at: 2504 2504
Size: 5963
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1520 1729 1520
Size: 209 5754
AlwNI CAG_nnn'CTG
Cuts at: 2722 3268 4835 2722
Size: 546 1567 3850
Fragments arranged by size:
3850 1567 546
ApaLI G'TGCA_C
Cuts at: 4733 4733
Size: 5963
ApoI r'AATT_y
Cuts at: 473 1103 1244 1360 2625 2967 5316 5572 473
Size: 630 141 116 1265 342 2349 256 864
Fragments arranged by size:
2349 1265 864 630 342 256 141 116
AseI AT'TA_AT
Cuts at: 20 20
Size: 5963
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5963
AvaI C'yCGr_G
Cuts at: 237 2983 237
Size: 2746 3217
BamHI G'GATC_C
Cuts at: 2988 2988
Size: 5963
BanI G'GyrC_C
Cuts at: 2979 3393 3641 3676 2979
Size: 414 248 35 5266
Fragments arranged by size:
5266 414 248 35
BanII G_rGCy'C
Cuts at: 163 173 2362 2977 4007 163
Size: 10 2189 615 1030 2119
Fragments arranged by size:
2189 2119 1030 615 10
BbeI G_GCGC'C
Cuts at: 3397 3645 3397
Size: 248 5715
BbsI GAAGACnn'nnnn_
Cuts at: 577 1667 2729 577
Size: 1090 1062 3811
Fragments arranged by size:
3811 1090 1062
BbvCI CC'TCA_GC
Cuts at: 252 1826 2612 252
Size: 1574 786 3603
Fragments arranged by size:
3603 1574 786
BciVI GTATCCnnnnn_n'
Cuts at: 552 1027 3861 4622 5963 552
Size: 475 2834 761 1341 552
Fragments arranged by size:
2834 1341 761 552 475
BclI T'GATC_A
Cuts at: 1802 2799 3483 1802
Size: 997 684 4282
Fragments arranged by size:
4282 997 684
BfrBI ATG'CAT
Cuts at: 916 916
Size: 5963
BglI GCCn_nnn'nGGC
Cuts at: 136 288 136
Size: 152 5811
BglII A'GATC_T
Cuts at: 3478 5515 3478
Size: 2037 3926
BlpI GC'TnA_GC
Cuts at: 1493 3075 1493
Size: 1582 4381
Bme1580I G_kGCm'C
Cuts at: 3588 3681 4737 3588
Size: 93 1056 4814
Fragments arranged by size:
4814 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1208 1481 2841 3175 3256 3591 5735 1208
Size: 273 1360 334 81 335 2144 1436
Fragments arranged by size:
2144 1436 1360 335 334 273 81
BmtI G_CTAG'C
Cuts at: 2065 2065
Size: 5963
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4330 4330
Size: 5963
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 732 1559 1967 2057 197
Size: 535 827 408 90 4103
Fragments arranged by size:
4103 827 535 408 90
Bpu10I CC'TnA_GC
Cuts at: 252 1826 2612 252
Size: 1574 786 3603
Fragments arranged by size:
3603 1574 786
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 442 646 1839 2852 3139 4018 4510 4808
Size: 144 204 1193 1013 287 879 492 298
Cuts at: 4808 298
Size: 1453
Fragments arranged by size:
1453 1193 1013 879 492 298 287 204
144
BsaI GGTCTCn'nnnn_
Cuts at: 173 2702 5667 173
Size: 2529 2965 469
Fragments arranged by size:
2965 2529 469
BsaAI yAC'GTr
Cuts at: 2119 2955 3316 2119
Size: 836 361 4766
Fragments arranged by size:
4766 836 361
BsaBI GATnn'nnATC
Cuts at: 6 3477 5514 6
Size: 3471 2037 455
Fragments arranged by size:
3471 2037 455
BsaHI Gr'CG_yC
Cuts at: 3394 3642 4344 5840 3394
Size: 248 702 1496 3517
Fragments arranged by size:
3517 1496 702 248
BsaWI w'CCGG_w
Cuts at: 721 1544 1872 2325 3152 3673 4351 4625 4772
Size: 823 328 453 827 521 678 274 147
Cuts at: 4772 5183 721
Size: 411 1501
Fragments arranged by size:
1501 827 823 678 521 453 411 328
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 569 599 992 1022 569
Size: 30 393 30 5510
Fragments arranged by size:
5510 393 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1031 1031
Size: 5963
BseYI C'CCAG_C
Cuts at: 3388 4146 4723 5219 3388
Size: 758 577 496 4132
Fragments arranged by size:
4132 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 1535 2675 322
Size: 1213 1140 3610
Fragments arranged by size:
3610 1213 1140
BsiEI CG_ry'CG
Cuts at: 104 3505 3551 4759 104
Size: 3401 46 1208 1308
Fragments arranged by size:
3401 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 163 173 1452 1494 2977 3755 3945 4737 163
Size: 10 1279 42 1483 778 190 792 1389
Fragments arranged by size:
1483 1389 1279 792 778 190 42 10
BsmI GAATG_Cn'
Cuts at: 875 1470 875
Size: 595 5368
Bsp1286I G_dGCh'C
Cuts at: 163 173 1452 1494 2362 2977 3588 3681 3755
Size: 10 1279 42 868 615 611 93 74
Cuts at: 3755 3945 4007 4737 163
Size: 190 62 730 1389
Fragments arranged by size:
1389 1279 868 730 615 611 190 93
74 62 42 10
BspEI T'CCGG_A
Cuts at: 1872 5183 1872
Size: 3311 2652
BspHI T'CATG_A
Cuts at: 2889 5139 2889
Size: 2250 3713
BspMI ACCTGCnnnn'nnnn_
Cuts at: 882 3013 3227 3529 3910 882
Size: 2131 214 302 381 2935
Fragments arranged by size:
2935 2131 381 302 214
BsrBI CCG'CTC
Cuts at: 50 791 4255 4309 5793 5958 50
Size: 741 3464 54 1484 165 55
Fragments arranged by size:
3464 1484 741 165 55 54
BsrDI GCAATG_nn'
Cuts at: 531 3875 531
Size: 3344 2619
BsrFI r'CCGG_y
Cuts at: 3961 4142 4351 3961
Size: 181 209 5573
Fragments arranged by size:
5573 209 181
BssSI C'ACGA_G
Cuts at: 4234 4592 4234
Size: 358 5605
BstAPI GCAn_nnn'nTGC
Cuts at: 1618 3340 1618
Size: 1722 4241
BstBI TT'CG_AA
Cuts at: 110 4324 110
Size: 4214 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1027 2032 1027
Size: 1005 4958
BstYI r'GATC_y
Cuts at: 1 775 2269 2988 3455 3478 3813 4059 5071
Size: 774 1494 719 467 23 335 246 1012
Cuts at: 5071 5157 5169 5515 1
Size: 86 12 346 449
Fragments arranged by size:
1494 1012 774 719 467 449 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1661 1746 1661
Size: 85 5878
BtgI C'CryG_G
Cuts at: 125 125
Size: 5963
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3913 4094 4257 3913
Size: 181 163 5619
Fragments arranged by size:
5619 181 163
BtsI GCAGTG_nn'
Cuts at: 3206 3206
Size: 5963
DraI TTT'AAA
Cuts at: 1232 2928 2962 1232
Size: 1696 34 4233
Fragments arranged by size:
4233 1696 34
DraIII CAC_nnn'GTG
Cuts at: 1177 1177
Size: 5963
DrdI GACnn_nn'nnGTC
Cuts at: 3669 4527 3669
Size: 858 5105
EaeI y'GGCC_r
Cuts at: 3548 3722 4113 4140 5485 3548
Size: 174 391 27 1345 4026
Fragments arranged by size:
4026 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3548 3548
Size: 5963
EarI CTCTTCn'nnn_
Cuts at: 192 781 998 1088 1319 2024 2423 2573 2675
Size: 589 217 90 231 705 399 150 102
Cuts at: 2675 3986 4196 192
Size: 1311 210 1959
Fragments arranged by size:
1959 1311 705 589 399 231 217 210
150 102 90
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4171 4481 4627 5773 4171
Size: 310 146 1146 4361
Fragments arranged by size:
4361 1146 310 146
EcoICRI GAG'CTC
Cuts at: 161 171 2975 161
Size: 10 2804 3149
Fragments arranged by size:
3149 2804 10
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 732 1148 1322 1438 1559 1883 1901 1967
Size: 535 416 174 116 121 324 18 66
Cuts at: 1967 2057 3788 4220 4967 5235 197
Size: 90 1731 432 747 268 925
Fragments arranged by size:
1731 925 747 535 432 416 324 268
174 121 116 90 66 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2578 2578
Size: 5963
EcoO109I rG'GnC_Cy
Cuts at: 2384 2454 3102 2384
Size: 70 648 5245
Fragments arranged by size:
5245 648 70
EcoRI G'AATT_C
Cuts at: 2967 2967
Size: 5963
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1330 3982 1330
Size: 2652 3311
FspI TGC'GCA
Cuts at: 1956 3744 1956
Size: 1788 4175
HaeII r_GCGC'y
Cuts at: 507 1481 3397 3645 4667 5646 507
Size: 974 1916 248 1022 979 824
Fragments arranged by size:
1916 1022 979 974 824 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 569 601 927 959 1692 1724 2490 2522 3805
Size: 32 326 32 733 32 766 32 1283
Cuts at: 3805 3835 3837 3867 569
Size: 30 2 30 2665
Fragments arranged by size:
2665 1283 766 733 326 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2945 3002 2945
Size: 57 5906
HindIII A'AGCT_T
Cuts at: 664 988 664
Size: 324 5639
HpaI GTT'AAC
Cuts at: 2945 2945
Size: 5963
KasI G'GCGC_C
Cuts at: 3393 3641 3393
Size: 248 5715
KpnI G_GTAC'C
Cuts at: 2983 2983
Size: 5963
MluI A'CGCG_T
Cuts at: 5396 5396
Size: 5963
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 612 663 992 1863 2218 2688 4634 4818 612
Size: 51 329 871 355 470 1946 184 1757
Fragments arranged by size:
1946 1757 871 470 355 329 184 51
MscI TGG'CCA
Cuts at: 3724 3724
Size: 5963
MslI CAynn'nnrTG
Cuts at: 3952 4079 3952
Size: 127 5836
MspA1I CmG'CkG
Cuts at: 594 2028 3074 3388 3748 4761 5006 594
Size: 1434 1046 314 360 1013 245 1551
Fragments arranged by size:
1551 1434 1046 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4144 4144
Size: 5963
NarI GG'CG_CC
Cuts at: 3394 3642 3394
Size: 248 5715
NcoI C'CATG_G
Cuts at: 125 125
Size: 5963
NdeI CA'TA_TG
Cuts at: 918 1807 918
Size: 889 5074
NgoMIV G'CCGG_C
Cuts at: 4142 4142
Size: 5963
NheI G'CTAG_C
Cuts at: 2061 2061
Size: 5963
NsiI A_TGCA'T
Cuts at: 918 918
Size: 5963
NspI r_CATG'y
Cuts at: 1185 2201 3016 3516 4423 1185
Size: 1016 815 500 907 2725
Fragments arranged by size:
2725 1016 907 815 500
PciI A'CATG_T
Cuts at: 4419 4419
Size: 5963
PfoI T'CCnGG_A
Cuts at: 879 1977 879
Size: 1098 4865
PmeI GTTT'AAAC
Cuts at: 2962 2962
Size: 5963
PmlI CAC'GTG
Cuts at: 2119 2119
Size: 5963
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 440 472 5102 5134 440
Size: 32 4630 32 1269
Fragments arranged by size:
4630 1269 32 32
PpuMI rG'GwC_Cy
Cuts at: 2384 2454 2384
Size: 70 5893
PshAI GACnn'nnGTC
Cuts at: 577 577
Size: 5963
PstI C_TGCA'G
Cuts at: 1614 3010 3695 1614
Size: 1396 685 3882
Fragments arranged by size:
3882 1396 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5963
PvuII CAG'CTG
Cuts at: 2028 3388 3748 2028
Size: 1360 360 4243
Fragments arranged by size:
4243 1360 360
RsrII CG'GwC_CG
Cuts at: 4158 4158
Size: 5963
SacI G_AGCT'C
Cuts at: 163 173 2977 163
Size: 10 2804 3149
Fragments arranged by size:
3149 2804 10
SalI G'TCGA_C
Cuts at: 3000 3000
Size: 5963
SapI GCTCTTCn'nnn_
Cuts at: 781 1088 3986 4196 781
Size: 307 2898 210 2548
Fragments arranged by size:
2898 2548 307 210
SbfI CC_TGCA'GG
Cuts at: 3010 3010
Size: 5963
ScaI AGT'ACT
Cuts at: 1520 5480 1520
Size: 3960 2003
SfcI C'TryA_G
Cuts at: 33 1066 1610 2256 3006 3691 4684 4875 33
Size: 1033 544 646 750 685 993 191 1121
Fragments arranged by size:
1121 1033 993 750 685 646 544 191
SfoI GGC'GCC
Cuts at: 3395 3643 3395
Size: 248 5715
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5963
SmaI CCC'GGG
Cuts at: 2985 2985
Size: 5963
SmlI C'TyrA_G
Cuts at: 277 457 661 1854 2392 2867 3118 4033 4525
Size: 180 204 1193 538 475 251 915 492
Cuts at: 4525 4787 277
Size: 262 1453
Fragments arranged by size:
1453 1193 915 538 492 475 262 251
204 180
SnaBI TAC'GTA
Cuts at: 2955 2955
Size: 5963
SpeI A'CTAG_T
Cuts at: 67 1213 67
Size: 1146 4817
SphI G_CATG'C
Cuts at: 3016 3516 3016
Size: 500 5463
StyI C'CwwG_G
Cuts at: 125 1718 3097 3451 125
Size: 1593 1379 354 2637
Fragments arranged by size:
2637 1593 1379 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3546 3679 4138 4347 3546
Size: 133 459 209 5162
Fragments arranged by size:
5162 459 209 133
TatI w'GTAC_w
Cuts at: 1518 1932 5478 1518
Size: 414 3546 2003
Fragments arranged by size:
3546 2003 414
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5480 5512 5480
Size: 32 5931
Tth111I GACn'n_nGTC
Cuts at: 963 2896 3760 963
Size: 1933 864 3166
Fragments arranged by size:
3166 1933 864
XbaI T'CTAG_A
Cuts at: 2994 2994
Size: 5963
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 3458 132
Size: 3326 2637
XmaI C'CCGG_G
Cuts at: 2983 2983
Size: 5963
XmnI GAAnn'nnTTC
Cuts at: 1307 1820 1868 1307
Size: 513 48 5402
Fragments arranged by size:
5402 513 48
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NsiI NspI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AleI AloI ApaI AscI AvrII BaeI BcgI
BmgBI BsiWI BsmBI BsrGI BssHII BstEII Bsu36I ClaI
CspCI EcoRV FseI FspAI MfeI NotI NruI PacI
PasI PflMI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI SspI StuI SwaI XhoI
ZraI