Restriction map for
pF1KSDA1290
(Circular) (Six-base) MAPSORT of: pF1KSDA1290.seq Check: 1241 from: 1 to: 6167
pF1KSDA1290 6167 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2763 2763
Size: 6167
AatII G_ACGT'C
Cuts at: 197 197
Size: 6167
AccI GT'mk_AC
Cuts at: 3205 3205
Size: 6167
Acc65I G'GTAC_C
Cuts at: 1393 3183 1393
Size: 1790 4377
AclI AA'CG_TT
Cuts at: 6007 6007
Size: 6167
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 330 601 1717 2101 2762 3121 3992 4424 5171
Size: 271 1116 384 661 359 871 432 747
Cuts at: 5171 5439 330
Size: 268 1058
Fragments arranged by size:
1116 1058 871 747 661 432 384 359
271 268
AflIII A'CryG_T
Cuts at: 2182 3519 4623 5600 2182
Size: 1337 1104 977 2749
Fragments arranged by size:
2749 1337 1104 977
AgeI A'CCGG_T
Cuts at: 4555 4555
Size: 6167
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2113 2431 2113
Size: 318 5849
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 739 771 739
Size: 32 6135
AlwNI CAG_nnn'CTG
Cuts at: 483 1531 2622 2844 3058 3472 5039 483
Size: 1048 1091 222 214 414 1567 1611
Fragments arranged by size:
1611 1567 1091 1048 414 222 214
ApaI G_GGCC'C
Cuts at: 2449 2766 2449
Size: 317 5850
ApaLI G'TGCA_C
Cuts at: 1399 4937 1399
Size: 3538 2629
ApoI r'AATT_y
Cuts at: 3171 5520 5776 3171
Size: 2349 256 3562
Fragments arranged by size:
3562 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6167
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6167
AvaI C'yCGr_G
Cuts at: 1481 3187 1481
Size: 1706 4461
BamHI G'GATC_C
Cuts at: 785 1910 3192 785
Size: 1125 1282 3760
Fragments arranged by size:
3760 1282 1125
BanI G'GyrC_C
Cuts at: 710 1393 1686 2144 2675 3183 3597 3845 3880
Size: 683 293 458 531 508 414 248 35
Cuts at: 3880 710
Size: 2997
Fragments arranged by size:
2997 683 531 508 458 414 293 248
35
BanII G_rGCy'C
Cuts at: 676 1117 1643 1883 2449 2766 3181 4211 676
Size: 441 526 240 566 317 415 1030 2632
Fragments arranged by size:
2632 1030 566 526 441 415 317 240
BbeI G_GCGC'C
Cuts at: 714 3601 3849 714
Size: 2887 248 3032
Fragments arranged by size:
3032 2887 248
BbsI GAAGACnn'nnnn_
Cuts at: 887 1252 887
Size: 365 5802
BbvCI CC'TCA_GC
Cuts at: 136 2671 136
Size: 2535 3632
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2547 2581 2547
Size: 34 6133
BciVI GTATCCnnnnn_n'
Cuts at: 2721 3108 4065 4826 6167 2721
Size: 387 957 761 1341 2721
Fragments arranged by size:
2721 1341 957 761 387
BclI T'GATC_A
Cuts at: 581 2903 3687 581
Size: 2322 784 3061
Fragments arranged by size:
3061 2322 784
BglI GCCn_nnn'nGGC
Cuts at: 929 1619 929
Size: 690 5477
BglII A'GATC_T
Cuts at: 1070 2879 3682 5719 1070
Size: 1809 803 2037 1518
Fragments arranged by size:
2037 1809 1518 803
BlpI GC'TnA_GC
Cuts at: 383 2104 3279 383
Size: 1721 1175 3271
Fragments arranged by size:
3271 1721 1175
Bme1580I G_kGCm'C
Cuts at: 572 1403 1732 1961 2131 2449 2766 3792 3885
Size: 831 329 229 170 318 317 1026 93
Cuts at: 3885 4941 572
Size: 1056 1798
Fragments arranged by size:
1798 1056 1026 831 329 318 317 229
170 93
BmgBI CAC'GTC
Cuts at: 1404 1476 2183 1404
Size: 72 707 5388
Fragments arranged by size:
5388 707 72
BmrI ACTGGGnnnn_n'
Cuts at: 2052 2316 2343 3379 3460 3795 5939 2052
Size: 264 27 1036 81 335 2144 2280
Fragments arranged by size:
2280 2144 1036 335 264 81 27
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2898 4534 2898
Size: 1636 4531
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 255 736 766 810 946 1084 1117 1237 1984
Size: 481 30 44 136 138 33 120 747
Cuts at: 1984 2866 2893 3115 255
Size: 882 27 222 3307
Fragments arranged by size:
3307 882 747 481 222 138 136 120
44 33 30 27
Bpu10I CC'TnA_GC
Cuts at: 136 1363 2671 2768 136
Size: 1227 1308 97 3535
Fragments arranged by size:
3535 1308 1227 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 414 940 3343 4222 4714 5012 414
Size: 526 2403 879 492 298 1569
Fragments arranged by size:
2403 1569 879 526 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 796 1345 5871 796
Size: 549 4526 1092
Fragments arranged by size:
4526 1092 549
BsaAI yAC'GTr
Cuts at: 2097 2604 3159 3520 2097
Size: 507 555 361 4744
Fragments arranged by size:
4744 555 507 361
BsaBI GATnn'nnATC
Cuts at: 6 3681 5718 6
Size: 3675 2037 455
Fragments arranged by size:
3675 2037 455
BsaHI Gr'CG_yC
Cuts at: 194 711 1319 3598 3846 4548 6044 194
Size: 517 608 2279 248 702 1496 317
Fragments arranged by size:
2279 1496 702 608 517 317 248
BsaWI w'CCGG_w
Cuts at: 1175 1775 2030 3356 3877 4555 4829 4976 5387
Size: 600 255 1326 521 678 274 147 411
Cuts at: 5387 1175
Size: 1955
Fragments arranged by size:
1955 1326 678 600 521 411 274 255
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 274 304 544 574 739 769 1099 1129 1360
Size: 30 240 30 165 30 330 30 231
Cuts at: 1360 1390 2308 2338 274
Size: 30 918 30 4103
Fragments arranged by size:
4103 918 330 240 231 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 298 847 1446 1801 2863 2971 298
Size: 549 599 355 1062 108 3494
Fragments arranged by size:
3494 1062 599 549 355 108
BseYI C'CCAG_C
Cuts at: 521 1652 1899 2274 3051 3592 4350 4927 5423
Size: 1131 247 375 777 541 758 577 496
Cuts at: 5423 521
Size: 1265
Fragments arranged by size:
1265 1131 777 758 577 541 496 375
247
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 170 496 1258 1566 2778 2797 3044 170
Size: 326 762 308 1212 19 247 3293
Fragments arranged by size:
3293 1212 762 326 308 247 19
BsiEI CG_ry'CG
Cuts at: 104 1472 1776 3709 3755 4963 104
Size: 1368 304 1933 46 1208 1308
Fragments arranged by size:
1933 1368 1308 1208 304 46
BsiHKAI G_wGCw'C
Cuts at: 1403 2456 2963 3181 3959 4149 4941 1403
Size: 1053 507 218 778 190 792 2629
Fragments arranged by size:
2629 1053 792 778 507 218 190
BsmI GAATG_Cn'
Cuts at: 539 1518 1997 2518 539
Size: 979 479 521 4188
Fragments arranged by size:
4188 979 521 479
Bsp1286I G_dGCh'C
Cuts at: 572 676 1117 1403 1643 1732 1883 1961 2131
Size: 104 441 286 240 89 151 78 170
Cuts at: 2131 2449 2456 2766 2963 3181 3792 3885 3959
Size: 318 7 310 197 218 611 93 74
Cuts at: 3959 4149 4211 4941 572
Size: 190 62 730 1798
Fragments arranged by size:
1798 730 611 441 318 310 286 240
218 197 190 170 151 104 93 89
78 74 62 7
BspEI T'CCGG_A
Cuts at: 5387 5387
Size: 6167
BspHI T'CATG_A
Cuts at: 3002 5343 3002
Size: 2341 3826
BspMI ACCTGCnnnn'nnnn_
Cuts at: 209 407 1379 1790 2763 3217 3431 3733 4114
Size: 198 972 411 973 454 214 302 381
Cuts at: 4114 209
Size: 2262
Fragments arranged by size:
2262 973 972 454 411 381 302 214
198
BsrBI CCG'CTC
Cuts at: 50 1454 1659 2829 4459 4513 5997 6162 50
Size: 1404 205 1170 1630 54 1484 165 55
Fragments arranged by size:
1630 1484 1404 1170 205 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2499 4079 2499
Size: 1580 4587
BsrFI r'CCGG_y
Cuts at: 234 1613 2141 2377 2586 2612 4165 4346 4555
Size: 1379 528 236 209 26 1553 181 209
Cuts at: 4555 234
Size: 1846
Fragments arranged by size:
1846 1553 1379 528 236 209 209 181
26
BsrGI T'GTAC_A
Cuts at: 1664 1664
Size: 6167
BssHII G'CGCG_C
Cuts at: 3018 3018
Size: 6167
BssSI C'ACGA_G
Cuts at: 4438 4796 4438
Size: 358 5809
BstAPI GCAn_nnn'nTGC
Cuts at: 1895 2622 3544 1895
Size: 727 922 4518
Fragments arranged by size:
4518 922 727
BstBI TT'CG_AA
Cuts at: 110 4528 110
Size: 4418 1749
BstEII G'GTnAC_C
Cuts at: 508 508
Size: 6167
BstXI CCAn_nnnn'nTGG
Cuts at: 279 453 739 984 2304 279
Size: 174 286 245 1320 4142
Fragments arranged by size:
4142 1320 286 245 174
BstYI r'GATC_y
Cuts at: 1 500 785 1070 1673 1778 1910 1976 2618
Size: 499 285 285 603 105 132 66 642
Cuts at: 2618 2879 3192 3659 3682 4017 4263 5275 5361
Size: 261 313 467 23 335 246 1012 86
Cuts at: 5361 5373 5719 1
Size: 12 346 449
Fragments arranged by size:
1012 642 603 499 467 449 346 335
313 285 285 261 246 132 105 86
66 23 12
Bsu36I CC'TnA_GG
Cuts at: 1916 3013 1916
Size: 1097 5070
BtgI C'CryG_G
Cuts at: 388 1269 1904 2285 2427 388
Size: 881 635 381 142 4128
Fragments arranged by size:
4128 881 635 381 142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4117 4298 4461 4117
Size: 181 163 5823
Fragments arranged by size:
5823 181 163
BtsI GCAGTG_nn'
Cuts at: 781 1229 1554 1947 3410 781
Size: 448 325 393 1463 3538
Fragments arranged by size:
3538 1463 448 393 325
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 648 683 648
Size: 35 6132
DraI TTT'AAA
Cuts at: 3166 3166
Size: 6167
DraIII CAC_nnn'GTG
Cuts at: 516 2231 3093 516
Size: 1715 862 3590
Fragments arranged by size:
3590 1715 862
DrdI GACnn_nn'nnGTC
Cuts at: 3873 4731 3873
Size: 858 5309
EaeI y'GGCC_r
Cuts at: 236 1040 1332 1620 1946 2288 2379 2588 2858
Size: 804 292 288 326 342 91 209 270
Cuts at: 2858 3042 3752 3926 4317 4344 5689 236
Size: 184 710 174 391 27 1345 714
Fragments arranged by size:
1345 804 714 710 391 342 326 292
288 270 209 184 174 91 27
EagI C'GGCC_G
Cuts at: 3752 3752
Size: 6167
EarI CTCTTCn'nnn_
Cuts at: 4190 4400 4190
Size: 210 5957
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1120 4375 4685 4831 5977 1120
Size: 3255 310 146 1146 1310
Fragments arranged by size:
3255 1310 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3179 3179
Size: 6167
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 255 330 601 736 766 810 946 1084 1117
Size: 75 271 135 30 44 136 138 33
Cuts at: 1117 1237 1717 1984 2101 2762 2866 2893 3115
Size: 120 480 267 117 661 104 27 222
Cuts at: 3115 3121 3992 4424 5171 5439 255
Size: 6 871 432 747 268 983
Fragments arranged by size:
983 871 747 661 480 432 271 268
267 222 138 136 135 120 117 104
75 44 33 30 27 6
EcoNI CCTnn'n_nnAGG
Cuts at: 619 634 2080 2314 2821 619
Size: 15 1446 234 507 3965
Fragments arranged by size:
3965 1446 507 234 15
EcoO109I rG'GnC_Cy
Cuts at: 221 1059 2217 2763 2841 3048 3306 221
Size: 838 1158 546 78 207 258 3082
Fragments arranged by size:
3082 1158 838 546 258 207 78
EcoRI G'AATT_C
Cuts at: 3171 3171
Size: 6167
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2712 4186 2712
Size: 1474 4693
FspI TGC'GCA
Cuts at: 3948 3948
Size: 6167
HaeII r_GCGC'y
Cuts at: 714 3601 3849 4871 5850 714
Size: 2887 248 1022 979 1031
Fragments arranged by size:
2887 1031 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 403 435 492 524 544 576 1099 1131 1360
Size: 32 57 32 20 32 523 32 229
Cuts at: 1360 1392 2443 2475 2674 2706 4009 4039 4041
Size: 32 1051 32 199 32 1303 30 2
Cuts at: 4041 4071 403
Size: 30 2499
Fragments arranged by size:
2499 1303 1051 523 229 199 57 32
32 32 32 32 32 32 30 30
20 2
HincII GTy'rAC
Cuts at: 1413 2172 2574 3206 1413
Size: 759 402 632 4374
Fragments arranged by size:
4374 759 632 402
KasI G'GCGC_C
Cuts at: 710 3597 3845 710
Size: 2887 248 3032
Fragments arranged by size:
3032 2887 248
KpnI G_GTAC'C
Cuts at: 1397 3187 1397
Size: 1790 4377
MluI A'CGCG_T
Cuts at: 5600 5600
Size: 6167
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 284 415 1820 4838 5022 284
Size: 131 1405 3018 184 1429
Fragments arranged by size:
3018 1429 1405 184 131
MscI TGG'CCA
Cuts at: 1042 1334 1622 1948 2290 2860 3928 1042
Size: 292 288 326 342 570 1068 3281
Fragments arranged by size:
3281 1068 570 342 326 292 288
MslI CAynn'nnrTG
Cuts at: 1511 2095 4156 4283 1511
Size: 584 2061 127 3395
Fragments arranged by size:
3395 2061 584 127
MspA1I CmG'CkG
Cuts at: 135 525 648 2108 2643 2730 2781 3055 3278
Size: 390 123 1460 535 87 51 274 223
Cuts at: 3278 3592 3952 4965 5210 135
Size: 314 360 1013 245 1092
Fragments arranged by size:
1460 1092 1013 535 390 360 314 274
245 223 123 87 51
NaeI GCC'GGC
Cuts at: 1615 2614 4348 1615
Size: 999 1734 3434
Fragments arranged by size:
3434 1734 999
NarI GG'CG_CC
Cuts at: 711 3598 3846 711
Size: 2887 248 3032
Fragments arranged by size:
3032 2887 248
NcoI C'CATG_G
Cuts at: 2285 2427 2285
Size: 142 6025
NdeI CA'TA_TG
Cuts at: 3031 3031
Size: 6167
NgoMIV G'CCGG_C
Cuts at: 1613 2612 4346 1613
Size: 999 1734 3434
Fragments arranged by size:
3434 1734 999
NspI r_CATG'y
Cuts at: 1146 1898 1926 3220 3720 4627 1146
Size: 752 28 1294 500 907 2686
Fragments arranged by size:
2686 1294 907 752 500 28
PasI CC'CwG_GG
Cuts at: 534 1281 534
Size: 747 5420
PciI A'CATG_T
Cuts at: 4623 4623
Size: 6167
PflMI CCAn_nnn'nTGG
Cuts at: 1341 2433 2844 1341
Size: 1092 411 4664
Fragments arranged by size:
4664 1092 411
PfoI T'CCnGG_A
Cuts at: 2713 2713
Size: 6167
PmeI GTTT'AAAC
Cuts at: 3166 3166
Size: 6167
PmlI CAC'GTG
Cuts at: 2097 2604 2097
Size: 507 5660
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 739 771 1630 1662 5306 5338 739
Size: 32 859 32 3644 32 1568
Fragments arranged by size:
3644 1568 859 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 221 1059 2841 3048 221
Size: 838 1782 207 3340
Fragments arranged by size:
3340 1782 838 207
PspOMI G'GGCC_C
Cuts at: 2445 2762 2445
Size: 317 5850
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2959 2991 2959
Size: 32 6135
PstI C_TGCA'G
Cuts at: 426 1745 2486 3214 3899 426
Size: 1319 741 728 685 2694
Fragments arranged by size:
2694 1319 741 728 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6167
PvuII CAG'CTG
Cuts at: 135 525 648 3592 3952 135
Size: 390 123 2944 360 2350
Fragments arranged by size:
2944 2350 390 360 123
RsrII CG'GwC_CG
Cuts at: 4362 4362
Size: 6167
SacI G_AGCT'C
Cuts at: 3181 3181
Size: 6167
SalI G'TCGA_C
Cuts at: 3204 3204
Size: 6167
SanDI GG'GwC_CC
Cuts at: 3048 3048
Size: 6167
SapI GCTCTTCn'nnn_
Cuts at: 4190 4400 4190
Size: 210 5957
SbfI CC_TGCA'GG
Cuts at: 1745 3214 1745
Size: 1469 4698
ScaI AGT'ACT
Cuts at: 5684 5684
Size: 6167
SexAI A'CCwGG_T
Cuts at: 1598 2816 3089 1598
Size: 1218 273 4676
Fragments arranged by size:
4676 1218 273
SfcI C'TryA_G
Cuts at: 33 422 1741 2482 3210 3895 4888 5079 33
Size: 389 1319 741 728 685 993 191 1121
Fragments arranged by size:
1319 1121 993 741 728 685 389 191
SfoI GGC'GCC
Cuts at: 712 3599 3847 712
Size: 2887 248 3032
Fragments arranged by size:
3032 2887 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6167
SgrAI Cr'CCGG_yG
Cuts at: 1613 1613
Size: 6167
SmaI CCC'GGG
Cuts at: 1483 3189 1483
Size: 1706 4461
SmlI C'TyrA_G
Cuts at: 429 955 3322 4237 4729 4991 429
Size: 526 2367 915 492 262 1605
Fragments arranged by size:
2367 1605 915 526 492 262
SnaBI TAC'GTA
Cuts at: 3159 3159
Size: 6167
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6167
SphI G_CATG'C
Cuts at: 3220 3720 3220
Size: 500 5667
StuI AGG'CCT
Cuts at: 3136 3136
Size: 6167
StyI C'CwwG_G
Cuts at: 1805 2285 2427 2525 3301 3655 1805
Size: 480 142 98 776 354 4317
Fragments arranged by size:
4317 776 480 354 142 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1486 1666 1945 2382 2693 3750 3883 4342 4551
Size: 180 279 437 311 1057 133 459 209
Cuts at: 4551 1486
Size: 3102
Fragments arranged by size:
3102 1057 459 437 311 279 209 180
133
TatI w'GTAC_w
Cuts at: 290 1664 2057 2807 5682 290
Size: 1374 393 750 2875 775
Fragments arranged by size:
2875 1374 775 750 393
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 179 211 5684 5716 179
Size: 32 5473 32 630
Fragments arranged by size:
5473 630 32 32
Tth111I GACn'n_nGTC
Cuts at: 1600 3964 1600
Size: 2364 3803
XbaI T'CTAG_A
Cuts at: 3198 3198
Size: 6167
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 453 597 2868 3662 453
Size: 144 2271 794 2958
Fragments arranged by size:
2958 2271 794 144
XmaI C'CCGG_G
Cuts at: 1481 3187 1481
Size: 1706 4461
ZraI GAC'GTC
Cuts at: 195 195
Size: 6167
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AscI AvrII BaeI BfrBI
BmtI BsiWI BsmBI BstZ17I ClaI EcoRV FseI FspAI
HindIII HpaI MfeI NheI NotI NruI NsiI PacI
PshAI PsiI PspXI SacII SfiI SrfI SspI SwaI
XhoI XmnI