Restriction map for
pF1KSDA1317
(Circular) (Six-base) MAPSORT of: pF1KSDA1317.seq Check: 394 from: 1 to: 4421
pF1KSDA1317 4421 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 393 393
Size: 4421
AccI GT'mk_AC
Cuts at: 1459 1459
Size: 4421
Acc65I G'GTAC_C
Cuts at: 1437 1437
Size: 4421
AclI AA'CG_TT
Cuts at: 4261 4261
Size: 4421
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1220 2246 2678 3425 3693 1220
Size: 1026 432 747 268 1948
Fragments arranged by size:
1948 1026 747 432 268
AflIII A'CryG_T
Cuts at: 1773 2877 3854 1773
Size: 1104 977 2340
Fragments arranged by size:
2340 1104 977
AgeI A'CCGG_T
Cuts at: 2809 2809
Size: 4421
AhdI GACnn_n'nnGTC
Cuts at: 1077 1077
Size: 4421
AlwNI CAG_nnn'CTG
Cuts at: 1149 1726 3293 1149
Size: 577 1567 2277
Fragments arranged by size:
2277 1567 577
ApaI G_GGCC'C
Cuts at: 1174 1174
Size: 4421
ApaLI G'TGCA_C
Cuts at: 3191 3191
Size: 4421
ApoI r'AATT_y
Cuts at: 523 811 1343 1425 3774 4030 523
Size: 288 532 82 2349 256 914
Fragments arranged by size:
2349 914 532 288 256 82
AseI AT'TA_AT
Cuts at: 20 20
Size: 4421
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4421
AvaI C'yCGr_G
Cuts at: 161 1441 161
Size: 1280 3141
BamHI G'GATC_C
Cuts at: 640 1446 640
Size: 806 3615
BanI G'GyrC_C
Cuts at: 1437 1851 2099 2134 1437
Size: 414 248 35 3724
Fragments arranged by size:
3724 414 248 35
BanII G_rGCy'C
Cuts at: 780 1174 1435 2465 780
Size: 394 261 1030 2736
Fragments arranged by size:
2736 1030 394 261
BbeI G_GCGC'C
Cuts at: 1855 2103 1855
Size: 248 4173
BbsI GAAGACnn'nnnn_
Cuts at: 444 942 444
Size: 498 3923
BciVI GTATCCnnnnn_n'
Cuts at: 1330 2319 3080 4421 1330
Size: 989 761 1341 1330
Fragments arranged by size:
1341 1330 989 761
BclI T'GATC_A
Cuts at: 417 1941 417
Size: 1524 2897
BglII A'GATC_T
Cuts at: 923 1936 3973 923
Size: 1013 2037 1371
Fragments arranged by size:
2037 1371 1013
BlpI GC'TnA_GC
Cuts at: 1533 1533
Size: 4421
Bme1580I G_kGCm'C
Cuts at: 1174 2046 2139 3195 1174
Size: 872 93 1056 2400
Fragments arranged by size:
2400 1056 872 93
BmgBI CAC'GTC
Cuts at: 1040 1040
Size: 4421
BmrI ACTGGGnnnn_n'
Cuts at: 1633 1714 2049 4193 1633
Size: 81 335 2144 1861
Fragments arranged by size:
2144 1861 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1107 2788 1107
Size: 1681 2740
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 780 1227 1330 780
Size: 447 103 3871
Fragments arranged by size:
3871 447 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1597 2476 2968 3266 1597
Size: 879 492 298 2752
Fragments arranged by size:
2752 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 758 4125 758
Size: 3367 1054
BsaAI yAC'GTr
Cuts at: 1413 1774 1413
Size: 361 4060
BsaBI GATnn'nnATC
Cuts at: 6 259 1935 3972 6
Size: 253 1676 2037 455
Fragments arranged by size:
2037 1676 455 253
BsaHI Gr'CG_yC
Cuts at: 1852 2100 2802 4298 1852
Size: 248 702 1496 1975
Fragments arranged by size:
1975 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1223 1610 2131 2809 3083 3230 3641 1223
Size: 387 521 678 274 147 411 2003
Fragments arranged by size:
2003 678 521 411 387 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1315 1318 1315
Size: 3 4418
BseYI C'CCAG_C
Cuts at: 1846 2604 3181 3677 1846
Size: 758 577 496 2590
Fragments arranged by size:
2590 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 630 630
Size: 4421
BsiEI CG_ry'CG
Cuts at: 104 606 1963 2009 3217 104
Size: 502 1357 46 1208 1308
Fragments arranged by size:
1357 1308 1208 502 46
BsiHKAI G_wGCw'C
Cuts at: 1435 2213 2403 3195 1435
Size: 778 190 792 2661
Fragments arranged by size:
2661 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1096 1096
Size: 4421
Bsp1286I G_dGCh'C
Cuts at: 780 1174 1435 2046 2139 2213 2403 2465 3195
Size: 394 261 611 93 74 190 62 730
Cuts at: 3195 780
Size: 2006
Fragments arranged by size:
2006 730 611 394 261 190 93 74
62
BspEI T'CCGG_A
Cuts at: 1223 3641 1223
Size: 2418 2003
BspHI T'CATG_A
Cuts at: 3597 3597
Size: 4421
BspMI ACCTGCnnnn'nnnn_
Cuts at: 393 1471 1685 1987 2368 393
Size: 1078 214 302 381 2446
Fragments arranged by size:
2446 1078 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1034 2713 2767 4251 4416 50
Size: 984 1679 54 1484 165 55
Fragments arranged by size:
1679 1484 984 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1053 2333 1053
Size: 1280 3141
BsrFI r'CCGG_y
Cuts at: 2419 2600 2809 2419
Size: 181 209 4031
Fragments arranged by size:
4031 209 181
BssSI C'ACGA_G
Cuts at: 2692 3050 2692
Size: 358 4063
BstAPI GCAn_nnn'nTGC
Cuts at: 1043 1798 1043
Size: 755 3666
BstBI TT'CG_AA
Cuts at: 110 2782 110
Size: 2672 1749
BstEII G'GTnAC_C
Cuts at: 974 974
Size: 4421
BstYI r'GATC_y
Cuts at: 1 640 923 1446 1913 1936 2271 2517 3529
Size: 639 283 523 467 23 335 246 1012
Cuts at: 3529 3615 3627 3973 1
Size: 86 12 346 449
Fragments arranged by size:
1012 639 523 467 449 346 335 283
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 197 197
Size: 4421
BtgI C'CryG_G
Cuts at: 125 125
Size: 4421
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2371 2552 2715 2371
Size: 181 163 4077
Fragments arranged by size:
4077 181 163
BtsI GCAGTG_nn'
Cuts at: 984 1664 984
Size: 680 3741
DraI TTT'AAA
Cuts at: 1420 1420
Size: 4421
DraIII CAC_nnn'GTG
Cuts at: 867 867
Size: 4421
DrdI GACnn_nn'nnGTC
Cuts at: 2127 2985 2127
Size: 858 3563
EaeI y'GGCC_r
Cuts at: 2006 2180 2571 2598 3943 2006
Size: 174 391 27 1345 2484
Fragments arranged by size:
2484 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2006 2006
Size: 4421
EarI CTCTTCn'nnn_
Cuts at: 2444 2654 2444
Size: 210 4211
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1221 2629 2939 3085 4231 1221
Size: 1408 310 146 1146 1411
Fragments arranged by size:
1411 1408 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1433 1433
Size: 4421
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 780 1220 1227 1330 2246 2678 3425 3693 780
Size: 440 7 103 916 432 747 268 1508
Fragments arranged by size:
1508 916 747 440 432 268 103 7
EcoO109I rG'GnC_Cy
Cuts at: 1170 1171 1560 1170
Size: 1 389 4031
Fragments arranged by size:
4031 389 1
EcoRI G'AATT_C
Cuts at: 523 1425 523
Size: 902 3519
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2440 2440
Size: 4421
FspI TGC'GCA
Cuts at: 2202 2202
Size: 4421
HaeII r_GCGC'y
Cuts at: 1855 2103 3125 4104 1855
Size: 248 1022 979 2172
Fragments arranged by size:
2172 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 791 823 829 861 1063 1095 1356 1388 2263
Size: 32 6 32 202 32 261 32 875
Cuts at: 2263 2293 2295 2325 791
Size: 30 2 30 2887
Fragments arranged by size:
2887 875 261 202 32 32 32 32
30 30 6 2
HincII GTy'rAC
Cuts at: 1460 1460
Size: 4421
KasI G'GCGC_C
Cuts at: 1851 2099 1851
Size: 248 4173
KpnI G_GTAC'C
Cuts at: 1441 1441
Size: 4421
MfeI C'AATT_G
Cuts at: 1295 1295
Size: 4421
MluI A'CGCG_T
Cuts at: 3854 3854
Size: 4421
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1212 3092 3276 1212
Size: 1880 184 2357
Fragments arranged by size:
2357 1880 184
MscI TGG'CCA
Cuts at: 2182 2182
Size: 4421
MslI CAynn'nnrTG
Cuts at: 625 2410 2537 625
Size: 1785 127 2509
Fragments arranged by size:
2509 1785 127
MspA1I CmG'CkG
Cuts at: 1182 1532 1846 2206 3219 3464 1182
Size: 350 314 360 1013 245 2139
Fragments arranged by size:
2139 1013 360 350 314 245
NaeI GCC'GGC
Cuts at: 2602 2602
Size: 4421
NarI GG'CG_CC
Cuts at: 1852 2100 1852
Size: 248 4173
NcoI C'CATG_G
Cuts at: 125 125
Size: 4421
NgoMIV G'CCGG_C
Cuts at: 2600 2600
Size: 4421
NspI r_CATG'y
Cuts at: 1474 1974 2881 1474
Size: 500 907 3014
Fragments arranged by size:
3014 907 500
PciI A'CATG_T
Cuts at: 2877 2877
Size: 4421
PflMI CCAn_nnn'nTGG
Cuts at: 867 1149 867
Size: 282 4139
PfoI T'CCnGG_A
Cuts at: 1322 1322
Size: 4421
PmeI GTTT'AAAC
Cuts at: 1420 1420
Size: 4421
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 114 146 3560 3592 114
Size: 32 3414 32 943
Fragments arranged by size:
3414 943 32 32
PspOMI G'GGCC_C
Cuts at: 1170 1170
Size: 4421
PstI C_TGCA'G
Cuts at: 1468 2153 1468
Size: 685 3736
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4421
PvuII CAG'CTG
Cuts at: 1182 1846 2206 1182
Size: 664 360 3397
Fragments arranged by size:
3397 664 360
RsrII CG'GwC_CG
Cuts at: 2616 2616
Size: 4421
SacI G_AGCT'C
Cuts at: 1435 1435
Size: 4421
SalI G'TCGA_C
Cuts at: 1458 1458
Size: 4421
SapI GCTCTTCn'nnn_
Cuts at: 2444 2654 2444
Size: 210 4211
SbfI CC_TGCA'GG
Cuts at: 1468 1468
Size: 4421
ScaI AGT'ACT
Cuts at: 3938 3938
Size: 4421
SfcI C'TryA_G
Cuts at: 33 144 1464 2149 3142 3333 33
Size: 111 1320 685 993 191 1121
Fragments arranged by size:
1320 1121 993 685 191 111
SfoI GGC'GCC
Cuts at: 1853 2101 1853
Size: 248 4173
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4421
SmaI CCC'GGG
Cuts at: 1443 1443
Size: 4421
SmlI C'TyrA_G
Cuts at: 161 1576 2491 2983 3245 161
Size: 1415 915 492 262 1337
Fragments arranged by size:
1415 1337 915 492 262
SnaBI TAC'GTA
Cuts at: 1413 1413
Size: 4421
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4421
SphI G_CATG'C
Cuts at: 1474 1974 1474
Size: 500 3921
StyI C'CwwG_G
Cuts at: 125 323 332 555 650 722 1375 1555 1909
Size: 198 9 223 95 72 653 180 354
Cuts at: 1909 125
Size: 2637
Fragments arranged by size:
2637 653 354 223 198 180 95 72
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2004 2137 2596 2805 2004
Size: 133 459 209 3620
Fragments arranged by size:
3620 459 209 133
TatI w'GTAC_w
Cuts at: 3936 3936
Size: 4421
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3938 3970 3938
Size: 32 4389
Tth111I GACn'n_nGTC
Cuts at: 478 2218 478
Size: 1740 2681
XbaI T'CTAG_A
Cuts at: 1452 1452
Size: 4421
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1916 1916
Size: 4421
XhoI C'TCGA_G
Cuts at: 161 161
Size: 4421
XmaI C'CCGG_G
Cuts at: 1441 1441
Size: 4421
XmnI GAAnn'nnTTC
Cuts at: 717 717
Size: 4421
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmtI Bpu10I BsaXI
BsiWI BsmI BsrGI BssHII BstXI BstZ17I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI SspI StuI SwaI ZraI