Restriction map for
pF1KSDA1318
(Circular) (Six-base) MAPSORT of: pF1KSDA1318.seq Check: 4528 from: 1 to: 7301
pF1KSDA1318 7301 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 213 775 1265 1399 4339 213
Size: 562 490 134 2940 3175
Fragments arranged by size:
3175 2940 562 490 134
Acc65I G'GTAC_C
Cuts at: 4317 4317
Size: 7301
AclI AA'CG_TT
Cuts at: 7141 7141
Size: 7301
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 269 506 941 1097 1175 1784 2057 2900 4018
Size: 237 435 156 78 609 273 843 1118
Cuts at: 4018 4082 5126 5558 6305 6573 269
Size: 64 1044 432 747 268 997
Fragments arranged by size:
1118 1044 997 843 747 609 435 432
273 268 237 156 78 64
AflIII A'CryG_T
Cuts at: 2774 4653 5757 6734 2774
Size: 1879 1104 977 3341
Fragments arranged by size:
3341 1879 1104 977
AgeI A'CCGG_T
Cuts at: 5689 5689
Size: 7301
AhdI GACnn_n'nnGTC
Cuts at: 345 345
Size: 7301
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3512 3512
Size: 7301
AlwNI CAG_nnn'CTG
Cuts at: 249 298 415 454 607 724 1075 1114 1153
Size: 49 117 39 153 117 351 39 39
Cuts at: 1153 1231 1309 1387 1426 1606 1684 1723 2035
Size: 78 78 78 39 180 78 39 312
Cuts at: 2035 2074 2113 2152 2191 2455 2533 2608 2689
Size: 39 39 39 39 264 78 75 81
Cuts at: 2689 2767 2806 2845 2923 3235 3274 3361 3430
Size: 78 39 39 78 312 39 87 69
Cuts at: 3430 3469 3967 4098 4606 6173 249
Size: 39 498 131 508 1567 1377
Fragments arranged by size:
1567 1377 508 498 351 312 312 264
180 153 131 117 117 87 81 78
78 78 78 78 78 78 75 69
49 39 39 39 39 39 39 39
39 39 39 39 39 39
ApaLI G'TGCA_C
Cuts at: 2778 6071 2778
Size: 3293 4008
ApoI r'AATT_y
Cuts at: 2364 4305 6654 6910 2364
Size: 1941 2349 256 2755
Fragments arranged by size:
2755 2349 1941 256
AseI AT'TA_AT
Cuts at: 20 706 2983 20
Size: 686 2277 4338
Fragments arranged by size:
4338 2277 686
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7301
AvaI C'yCGr_G
Cuts at: 3599 4321 3599
Size: 722 6579
BamHI G'GATC_C
Cuts at: 4326 4326
Size: 7301
BanI G'GyrC_C
Cuts at: 4317 4731 4979 5014 4317
Size: 414 248 35 6604
Fragments arranged by size:
6604 414 248 35
BanII G_rGCy'C
Cuts at: 188 1380 1638 1755 2082 2223 2526 2721 2799
Size: 1192 258 117 327 141 303 195 78
Cuts at: 2799 2838 2994 3607 4153 4315 5345 188
Size: 39 156 613 546 162 1030 2144
Fragments arranged by size:
2144 1192 1030 613 546 327 303 258
195 162 156 141 117 78 39
BbeI G_GCGC'C
Cuts at: 4735 4983 4735
Size: 248 7053
BbsI GAAGACnn'nnnn_
Cuts at: 842 4225 842
Size: 3383 3918
BbvCI CC'TCA_GC
Cuts at: 2877 2877
Size: 7301
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3231 3265 3231
Size: 34 7267
BciVI GTATCCnnnnn_n'
Cuts at: 5199 5960 7301 5199
Size: 761 1341 5199
Fragments arranged by size:
5199 1341 761
BclI T'GATC_A
Cuts at: 4239 4821 4239
Size: 582 6719
BglII A'GATC_T
Cuts at: 1026 1945 3121 4816 6853 1026
Size: 919 1176 1695 2037 1474
Fragments arranged by size:
2037 1695 1474 1176 919
BlpI GC'TnA_GC
Cuts at: 4413 4413
Size: 7301
Bme1580I G_kGCm'C
Cuts at: 423 462 2782 4926 5019 6075 423
Size: 39 2320 2144 93 1056 1649
Fragments arranged by size:
2320 2144 1649 1056 93 39
BmrI ACTGGGnnnn_n'
Cuts at: 4513 4594 4929 7073 4513
Size: 81 335 2144 4741
Fragments arranged by size:
4741 2144 335 81
BmtI G_CTAG'C
Cuts at: 715 715
Size: 7301
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1652 2484 3772 5668 1652
Size: 832 1288 1896 3285
Fragments arranged by size:
3285 1896 1288 832
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 320 398 668 746 785 902 980 1052
Size: 6 78 270 78 39 117 78 72
Cuts at: 1052 1130 1136 1214 1370 1448 1487 1565 1667
Size: 78 6 78 156 78 39 78 102
Cuts at: 1667 1862 1979 2096 2135 2174 2213 2330 2369
Size: 195 117 117 39 39 39 117 39
Cuts at: 2369 2477 2510 2516 2555 2672 2711 2789 2822
Size: 108 33 6 39 117 39 78 33
Cuts at: 2822 2828 2984 3062 3101 3218 3452 314
Size: 6 156 78 39 117 234 4163
Fragments arranged by size:
4163 270 234 195 156 156 117 117
117 117 117 117 108 102 78 78
78 78 78 78 78 78 78 72
39 39 39 39 39 39 39 39
39 33 33 6 6 6 6
Bpu10I CC'TnA_GC
Cuts at: 2877 2877
Size: 7301
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2207 4071 4477 5356 5848 6146 2207
Size: 1864 406 879 492 298 3362
Fragments arranged by size:
3362 1864 879 492 406 298
BsaI GGTCTCn'nnnn_
Cuts at: 220 2865 2964 4040 4211 7005 220
Size: 2645 99 1076 171 2794 516
Fragments arranged by size:
2794 2645 1076 516 171 99
BsaAI yAC'GTr
Cuts at: 4293 4654 4293
Size: 361 6940
BsaBI GATnn'nnATC
Cuts at: 6 758 1809 1887 3823 4815 6852 6
Size: 752 1051 78 1936 992 2037 455
Fragments arranged by size:
2037 1936 1051 992 752 455 78
BsaHI Gr'CG_yC
Cuts at: 4732 4980 5682 7178 4732
Size: 248 702 1496 4855
Fragments arranged by size:
4855 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3408 4490 5011 5689 5963 6110 6521 3408
Size: 1082 521 678 274 147 411 4188
Fragments arranged by size:
4188 1082 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1644 3655 4141 4183 1644
Size: 2011 486 42 4762
Fragments arranged by size:
4762 2011 486 42
BseYI C'CCAG_C
Cuts at: 796 974 1167 1380 1716 2562 2799 3561 4726
Size: 178 193 213 336 846 237 762 1165
Cuts at: 4726 5484 6061 6557 796
Size: 758 577 496 1540
Fragments arranged by size:
1540 1165 846 762 758 577 496 336
237 213 193 178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 1307 2924 335
Size: 972 1617 4712
Fragments arranged by size:
4712 1617 972
BsiEI CG_ry'CG
Cuts at: 104 4843 4889 6097 104
Size: 4739 46 1208 1308
Fragments arranged by size:
4739 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 676 1638 2082 2223 2299 2526 2782 2838 4153
Size: 962 444 141 76 227 256 56 1315
Cuts at: 4153 4175 4258 4315 5093 5283 6075 676
Size: 22 83 57 778 190 792 1902
Fragments arranged by size:
1902 1315 962 792 778 444 256 227
190 141 83 76 57 56 22
BsmI GAATG_Cn'
Cuts at: 252 252
Size: 7301
Bsp1286I G_dGCh'C
Cuts at: 188 423 462 676 1380 1638 1755 2082 2223
Size: 235 39 214 704 258 117 327 141
Cuts at: 2223 2299 2526 2721 2782 2799 2838 2994 3607
Size: 76 227 195 61 17 39 156 613
Cuts at: 3607 4153 4175 4258 4315 4926 5019 5093 5283
Size: 546 22 83 57 611 93 74 190
Cuts at: 5283 5345 6075 188
Size: 62 730 1414
Fragments arranged by size:
1414 730 704 613 611 546 327 258
235 227 214 195 190 156 141 117
93 83 76 74 62 61 57 39
39 22 17
BspEI T'CCGG_A
Cuts at: 3408 6521 3408
Size: 3113 4188
BspHI T'CATG_A
Cuts at: 4236 6477 4236
Size: 2241 5060
BspMI ACCTGCnnnn'nnnn_
Cuts at: 4351 4565 4867 5248 4351
Size: 214 302 381 6404
Fragments arranged by size:
6404 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1017 5593 5647 7131 7296 50
Size: 967 4576 54 1484 165 55
Fragments arranged by size:
4576 1484 967 165 55 54
BsrDI GCAATG_nn'
Cuts at: 430 469 508 635 698 815 817 854 1166
Size: 39 39 127 63 117 2 37 312
Cuts at: 1166 1361 1402 1556 1658 1775 1853 2048 2165
Size: 195 41 154 102 117 78 195 117
Cuts at: 2165 2663 3094 5213 430
Size: 498 431 2119 2518
Fragments arranged by size:
2518 2119 498 431 312 195 195 154
127 117 117 117 102 78 63 41
39 39 37 2
BsrFI r'CCGG_y
Cuts at: 5299 5480 5689 5299
Size: 181 209 6911
Fragments arranged by size:
6911 209 181
BsrGI T'GTAC_A
Cuts at: 261 658 4040 261
Size: 397 3382 3522
Fragments arranged by size:
3522 3382 397
BssSI C'ACGA_G
Cuts at: 5572 5930 5572
Size: 358 6943
BstAPI GCAn_nnn'nTGC
Cuts at: 4678 4678
Size: 7301
BstBI TT'CG_AA
Cuts at: 110 5662 110
Size: 5552 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 997 1879 2308 3948 3981 997
Size: 882 429 1640 33 4317
Fragments arranged by size:
4317 1640 882 429 33
BstYI r'GATC_y
Cuts at: 1 1026 1945 2304 2557 2677 3121 3787 3989
Size: 1025 919 359 253 120 444 666 202
Cuts at: 3989 4326 4793 4816 5151 5397 6409 6495 6507
Size: 337 467 23 335 246 1012 86 12
Cuts at: 6507 6853 1
Size: 346 449
Fragments arranged by size:
1025 1012 919 666 467 449 444 359
346 337 335 253 246 202 120 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1800 1917 1995 3354 1800
Size: 117 78 1359 5747
Fragments arranged by size:
5747 1359 117 78
BtgI C'CryG_G
Cuts at: 1246 1574 1793 2954 3032 3071 4133 1246
Size: 328 219 1161 78 39 1062 4414
Fragments arranged by size:
4414 1161 1062 328 219 78 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1034 1252 2114 3470 5251 5432 5595 1034
Size: 218 862 1356 1781 181 163 2740
Fragments arranged by size:
2740 1781 1356 862 218 181 163
BtsI GCAGTG_nn'
Cuts at: 145 2050 2470 2936 4544 145
Size: 1905 420 466 1608 2902
Fragments arranged by size:
2902 1905 1608 466 420
ClaI AT'CG_AT
Cuts at: 3241 3241
Size: 7301
DraI TTT'AAA
Cuts at: 4300 4300
Size: 7301
DraIII CAC_nnn'GTG
Cuts at: 4049 4049
Size: 7301
DrdI GACnn_nn'nnGTC
Cuts at: 5007 5865 5007
Size: 858 6443
EaeI y'GGCC_r
Cuts at: 1532 3167 4886 5060 5451 5478 6823 1532
Size: 1635 1719 174 391 27 1345 2010
Fragments arranged by size:
2010 1719 1635 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4886 4886
Size: 7301
EarI CTCTTCn'nnn_
Cuts at: 3578 3617 3704 4058 5324 5534 3578
Size: 39 87 354 1266 210 5345
Fragments arranged by size:
5345 1266 354 210 87 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1277 5509 5819 5965 7111 1277
Size: 4232 310 146 1146 1467
Fragments arranged by size:
4232 1467 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1636 2080 2221 2524 2836 4151 4313 1636
Size: 444 141 303 312 1315 162 4624
Fragments arranged by size:
4624 1315 444 312 303 162 141
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 269 314 320 398 506 668 746 785 902
Size: 45 6 78 108 162 78 39 117
Cuts at: 902 941 980 1052 1097 1130 1136 1175 1214
Size: 39 39 72 45 33 6 39 39
Cuts at: 1214 1370 1448 1487 1565 1667 1784 1862 1979
Size: 156 78 39 78 102 117 78 117
Cuts at: 1979 2057 2096 2135 2174 2213 2330 2369 2477
Size: 78 39 39 39 39 117 39 108
Cuts at: 2477 2510 2516 2555 2672 2711 2789 2822 2828
Size: 33 6 39 117 39 78 33 6
Cuts at: 2828 2900 2984 3062 3101 3218 3452 4018 4082
Size: 72 84 78 39 117 234 566 64
Cuts at: 4082 5126 5558 6305 6573 269
Size: 1044 432 747 268 997
Fragments arranged by size:
1044 997 747 566 432 268 234 162
156 117 117 117 117 117 117 108
108 102 84 78 78 78 78 78
78 78 78 72 72 64 45 45
39 39 39 39 39 39 39 39
39 39 39 39 39 39 33 33
33 6 6 6 6
EcoO109I rG'GnC_Cy
Cuts at: 2465 3350 3358 4440 2465
Size: 885 8 1082 5326
Fragments arranged by size:
5326 1082 885 8
EcoRI G'AATT_C
Cuts at: 2364 4305 2364
Size: 1941 5360
EcoRV GAT'ATC
Cuts at: 617 890 617
Size: 273 7028
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4249 5320 4249
Size: 1071 6230
FspI TGC'GCA
Cuts at: 5082 5082
Size: 7301
HaeII r_GCGC'y
Cuts at: 3178 4735 4983 6005 6984 3178
Size: 1557 248 1022 979 3495
Fragments arranged by size:
3495 1557 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 312 331 344 363 679 711 796 828 1018
Size: 19 13 19 316 32 85 32 190
Cuts at: 1018 1050 1269 1301 1474 1506 1742 1774 1900
Size: 32 219 32 173 32 236 32 126
Cuts at: 1900 1917 1932 1949 2095 2127 2268 2300 2371
Size: 17 15 17 146 32 141 32 71
Cuts at: 2371 2403 2878 2910 3390 3422 3796 3828 5143
Size: 32 475 32 480 32 374 32 1315
Cuts at: 5143 5173 5175 5205 312
Size: 30 2 30 2408
Fragments arranged by size:
2408 1315 480 475 374 316 236 219
190 173 146 141 126 85 71 32
32 32 32 32 32 32 32 32
32 32 32 30 30 19 19 17
17 15 13 2
HincII GTy'rAC
Cuts at: 1253 1526 3557 4340 1253
Size: 273 2031 783 4214
Fragments arranged by size:
4214 2031 783 273
HpaI GTT'AAC
Cuts at: 1526 1526
Size: 7301
KasI G'GCGC_C
Cuts at: 4731 4979 4731
Size: 248 7053
KpnI G_GTAC'C
Cuts at: 4321 4321
Size: 7301
MfeI C'AATT_G
Cuts at: 1705 1705
Size: 7301
MluI A'CGCG_T
Cuts at: 6734 6734
Size: 7301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1663 3967 5972 6156 1663
Size: 2304 2005 184 2808
Fragments arranged by size:
2808 2304 2005 184
MscI TGG'CCA
Cuts at: 1534 3169 5062 1534
Size: 1635 1893 3773
Fragments arranged by size:
3773 1893 1635
MslI CAynn'nnrTG
Cuts at: 257 3289 3979 4178 5290 5417 257
Size: 3032 690 199 1112 127 2141
Fragments arranged by size:
3032 2141 1112 690 199 127
MspA1I CmG'CkG
Cuts at: 949 2347 4056 4412 4726 5086 6099 6344 949
Size: 1398 1709 356 314 360 1013 245 1906
Fragments arranged by size:
1906 1709 1398 1013 360 356 314 245
NaeI GCC'GGC
Cuts at: 5482 5482
Size: 7301
NarI GG'CG_CC
Cuts at: 4732 4980 4732
Size: 248 7053
NcoI C'CATG_G
Cuts at: 1246 1793 2954 3032 3071 4133 1246
Size: 547 1161 78 39 1062 4414
Fragments arranged by size:
4414 1161 1062 547 78 39
NdeI CA'TA_TG
Cuts at: 2930 2930
Size: 7301
NgoMIV G'CCGG_C
Cuts at: 5480 5480
Size: 7301
NheI G'CTAG_C
Cuts at: 711 711
Size: 7301
NspI r_CATG'y
Cuts at: 2778 4183 4354 4854 5761 2778
Size: 1405 171 500 907 4318
Fragments arranged by size:
4318 1405 907 500 171
PasI CC'CwG_GG
Cuts at: 544 3792 544
Size: 3248 4053
PciI A'CATG_T
Cuts at: 2774 5757 2774
Size: 2983 4318
PflMI CCAn_nnn'nTGG
Cuts at: 376 646 724 1114 1231 1387 1426 1465 1645
Size: 270 78 390 117 156 39 39 180
Cuts at: 1645 1723 2533 2569 2806 2845 376
Size: 78 810 36 237 39 4832
Fragments arranged by size:
4832 810 390 270 237 180 156 117
78 78 39 39 39 36
PfoI T'CCnGG_A
Cuts at: 762 2307 762
Size: 1545 5756
PmeI GTTT'AAAC
Cuts at: 4300 4300
Size: 7301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3709 3741 6440 6472 3709
Size: 32 2699 32 4538
Fragments arranged by size:
4538 2699 32 32
PshAI GACnn'nnGTC
Cuts at: 2931 3912 2931
Size: 981 6320
PstI C_TGCA'G
Cuts at: 612 1818 4348 5033 612
Size: 1206 2530 685 2880
Fragments arranged by size:
2880 2530 1206 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7301
PvuII CAG'CTG
Cuts at: 949 4056 4726 5086 949
Size: 3107 670 360 3164
Fragments arranged by size:
3164 3107 670 360
RsrII CG'GwC_CG
Cuts at: 5496 5496
Size: 7301
SacI G_AGCT'C
Cuts at: 1638 2082 2223 2526 2838 4153 4315 1638
Size: 444 141 303 312 1315 162 4624
Fragments arranged by size:
4624 1315 444 312 303 162 141
SalI G'TCGA_C
Cuts at: 4338 4338
Size: 7301
SapI GCTCTTCn'nnn_
Cuts at: 3578 5324 5534 3578
Size: 1746 210 5345
Fragments arranged by size:
5345 1746 210
SbfI CC_TGCA'GG
Cuts at: 4348 4348
Size: 7301
ScaI AGT'ACT
Cuts at: 6818 6818
Size: 7301
SexAI A'CCwGG_T
Cuts at: 3940 3940
Size: 7301
SfcI C'TryA_G
Cuts at: 33 210 272 608 950 1814 4344 5029 6022
Size: 177 62 336 342 864 2530 685 993
Cuts at: 6022 6213 33
Size: 191 1121
Fragments arranged by size:
2530 1121 993 864 685 342 336 191
177 62
SfoI GGC'GCC
Cuts at: 4733 4981 4733
Size: 248 7053
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7301
SmaI CCC'GGG
Cuts at: 3601 4323 3601
Size: 722 6579
SmlI C'TyrA_G
Cuts at: 2222 4086 4456 5371 5863 6125 2222
Size: 1864 370 915 492 262 3398
Fragments arranged by size:
3398 1864 915 492 370 262
SnaBI TAC'GTA
Cuts at: 4293 4293
Size: 7301
SpeI A'CTAG_T
Cuts at: 67 2826 67
Size: 2759 4542
SphI G_CATG'C
Cuts at: 4354 4854 4354
Size: 500 6801
StyI C'CwwG_G
Cuts at: 1246 1793 2954 3032 3071 4133 4245 4435 4789
Size: 547 1161 78 39 1062 112 190 354
Cuts at: 4789 1246
Size: 3758
Fragments arranged by size:
3758 1161 1062 547 354 190 112 78
39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1003 1222 1675 2001 2314 4884 5017 5476 5685
Size: 219 453 326 313 2570 133 459 209
Cuts at: 5685 1003
Size: 2619
Fragments arranged by size:
2619 2570 459 453 326 313 219 209
133
TatI w'GTAC_w
Cuts at: 261 658 4040 6816 261
Size: 397 3382 2776 746
Fragments arranged by size:
3382 2776 746 397
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2289 2321 6818 6850 2289
Size: 32 4497 32 2740
Fragments arranged by size:
4497 2740 32 32
Tth111I GACn'n_nGTC
Cuts at: 2892 5098 2892
Size: 2206 5095
XbaI T'CTAG_A
Cuts at: 1029 1504 2728 3118 3322 4332 1029
Size: 475 1224 390 204 1010 3998
Fragments arranged by size:
3998 1224 1010 475 390 204
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1368 4796 1368
Size: 3428 3873
XmaI C'CCGG_G
Cuts at: 3599 4321 3599
Size: 722 6579
XmnI GAAnn'nnTTC
Cuts at: 4232 4232
Size: 7301
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BaeI BfrBI BglI BmgBI BsaXI BsiWI BsmBI
BssHII BstEII BstZ17I CspCI EcoNI FseI FspAI HindIII
NotI NruI NsiI PacI PmlI PpuMI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SrfI SspI
StuI SwaI XhoI ZraI