Restriction map for
pF1KSDA1322
(Circular) (Six-base) MAPSORT of: pF1KSDA1322.seq Check: 3167 from: 1 to: 5216
pF1KSDA1322 5216 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 189 2254 189
Size: 2065 3151
Acc65I G'GTAC_C
Cuts at: 2232 2232
Size: 5216
AclI AA'CG_TT
Cuts at: 5056 5056
Size: 5216
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 1130 1481 2014 3041 3473 4220 4488 190
Size: 940 351 533 1027 432 747 268 918
Fragments arranged by size:
1027 940 918 747 533 432 351 268
AflIII A'CryG_T
Cuts at: 219 1589 1940 2568 3672 4649 219
Size: 1370 351 628 1104 977 786
Fragments arranged by size:
1370 1104 977 786 628 351
AgeI A'CCGG_T
Cuts at: 3604 3604
Size: 5216
AlwNI CAG_nnn'CTG
Cuts at: 213 2049 2145 2521 4088 213
Size: 1836 96 376 1567 1341
Fragments arranged by size:
1836 1567 1341 376 96
ApaI G_GGCC'C
Cuts at: 269 269
Size: 5216
ApaLI G'TGCA_C
Cuts at: 3986 3986
Size: 5216
ApoI r'AATT_y
Cuts at: 1815 2220 4569 4825 1815
Size: 405 2349 256 2206
Fragments arranged by size:
2349 2206 405 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5216
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5216
AvaI C'yCGr_G
Cuts at: 321 415 1072 1418 2062 2236 321
Size: 94 657 346 644 174 3301
Fragments arranged by size:
3301 657 644 346 174 94
BamHI G'GATC_C
Cuts at: 2241 2241
Size: 5216
BanI G'GyrC_C
Cuts at: 234 2232 2646 2894 2929 234
Size: 1998 414 248 35 2521
Fragments arranged by size:
2521 1998 414 248 35
BanII G_rGCy'C
Cuts at: 269 745 1400 2139 2230 3260 269
Size: 476 655 739 91 1030 2225
Fragments arranged by size:
2225 1030 739 655 476 91
BbeI G_GCGC'C
Cuts at: 238 2650 2898 238
Size: 2412 248 2556
Fragments arranged by size:
2556 2412 248
BbsI GAAGACnn'nnnn_
Cuts at: 299 983 996 299
Size: 684 13 4519
Fragments arranged by size:
4519 684 13
BbvCI CC'TCA_GC
Cuts at: 794 794
Size: 5216
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2094 2128 2094
Size: 34 5182
BciVI GTATCCnnnnn_n'
Cuts at: 3114 3875 5216 3114
Size: 761 1341 3114
Fragments arranged by size:
3114 1341 761
BclI T'GATC_A
Cuts at: 2736 2736
Size: 5216
BglII A'GATC_T
Cuts at: 1265 2731 4768 1265
Size: 1466 2037 1713
Fragments arranged by size:
2037 1713 1466
BlpI GC'TnA_GC
Cuts at: 1362 2328 1362
Size: 966 4250
Bme1580I G_kGCm'C
Cuts at: 269 2841 2934 3990 269
Size: 2572 93 1056 1495
Fragments arranged by size:
2572 1495 1056 93
BmgBI CAC'GTC
Cuts at: 222 1941 222
Size: 1719 3497
BmrI ACTGGGnnnn_n'
Cuts at: 1287 2037 2428 2509 2844 4988 1287
Size: 750 391 81 335 2144 1515
Fragments arranged by size:
2144 1515 750 391 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3583 3583
Size: 5216
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 358 666 721 1377 1534 1844 292
Size: 66 308 55 656 157 310 3664
Fragments arranged by size:
3664 656 310 308 157 66 55
Bpu10I CC'TnA_GC
Cuts at: 794 1444 2138 794
Size: 650 694 3872
Fragments arranged by size:
3872 694 650
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 759 2392 3271 3763 4061 214
Size: 545 1633 879 492 298 1369
Fragments arranged by size:
1633 1369 879 545 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4920 4920
Size: 5216
BsaAI yAC'GTr
Cuts at: 381 2208 2569 381
Size: 1827 361 3028
Fragments arranged by size:
3028 1827 361
BsaBI GATnn'nnATC
Cuts at: 6 2730 4767 6
Size: 2724 2037 455
Fragments arranged by size:
2724 2037 455
BsaHI Gr'CG_yC
Cuts at: 235 2647 2895 3597 5093 235
Size: 2412 248 702 1496 358
Fragments arranged by size:
2412 1496 702 358 248
BsaWI w'CCGG_w
Cuts at: 2405 2926 3604 3878 4025 4436 2405
Size: 521 678 274 147 411 3185
Fragments arranged by size:
3185 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 399 811 2095 399
Size: 412 1284 3520
Fragments arranged by size:
3520 1284 412
BseYI C'CCAG_C
Cuts at: 1095 1184 2641 3399 3976 4472 1095
Size: 89 1457 758 577 496 1839
Fragments arranged by size:
1839 1457 758 577 496 89
BsiEI CG_ry'CG
Cuts at: 104 2758 2804 4012 104
Size: 2654 46 1208 1308
Fragments arranged by size:
2654 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 745 1250 1297 1400 2230 3008 3198 3990 745
Size: 505 47 103 830 778 190 792 1971
Fragments arranged by size:
1971 830 792 778 505 190 103 47
BsmI GAATG_Cn'
Cuts at: 942 942
Size: 5216
BsmBI CGTCTCn'nnnn_
Cuts at: 252 252
Size: 5216
Bsp1286I G_dGCh'C
Cuts at: 269 745 1250 1297 1400 2139 2230 2841 2934
Size: 476 505 47 103 739 91 611 93
Cuts at: 2934 3008 3198 3260 3990 269
Size: 74 190 62 730 1495
Fragments arranged by size:
1495 739 730 611 505 476 190 103
93 91 74 62 47
BspEI T'CCGG_A
Cuts at: 4436 4436
Size: 5216
BspHI T'CATG_A
Cuts at: 786 1584 4392 786
Size: 798 2808 1610
Fragments arranged by size:
2808 1610 798
BspMI ACCTGCnnnn'nnnn_
Cuts at: 221 2078 2266 2480 2782 3163 221
Size: 1857 188 214 302 381 2274
Fragments arranged by size:
2274 1857 381 302 214 188
BsrBI CCG'CTC
Cuts at: 50 420 483 3508 3562 5046 5211 50
Size: 370 63 3025 54 1484 165 55
Fragments arranged by size:
3025 1484 370 165 63 55 54
BsrDI GCAATG_nn'
Cuts at: 651 2152 3128 651
Size: 1501 976 2739
Fragments arranged by size:
2739 1501 976
BsrFI r'CCGG_y
Cuts at: 296 1625 3214 3395 3604 296
Size: 1329 1589 181 209 1908
Fragments arranged by size:
1908 1589 1329 209 181
BssHII G'CGCG_C
Cuts at: 440 440
Size: 5216
BssSI C'ACGA_G
Cuts at: 672 1393 3487 3845 672
Size: 721 2094 358 2043
Fragments arranged by size:
2094 2043 721 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2593 2593
Size: 5216
BstBI TT'CG_AA
Cuts at: 110 3577 110
Size: 3467 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1573 1573
Size: 5216
BstYI r'GATC_y
Cuts at: 1 1265 1883 2241 2708 2731 3066 3312 4324
Size: 1264 618 358 467 23 335 246 1012
Cuts at: 4324 4410 4422 4768 1
Size: 86 12 346 449
Fragments arranged by size:
1264 1012 618 467 449 358 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 314 759 1764 314
Size: 445 1005 3766
Fragments arranged by size:
3766 1005 445
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 593 1967 2082 3166 3347 3510 593
Size: 1374 115 1084 181 163 2299
Fragments arranged by size:
2299 1374 1084 181 163 115
BtsI GCAGTG_nn'
Cuts at: 1676 2459 1676
Size: 783 4433
DraI TTT'AAA
Cuts at: 2215 2215
Size: 5216
DrdI GACnn_nn'nnGTC
Cuts at: 621 667 2922 3780 621
Size: 46 2255 858 2057
Fragments arranged by size:
2255 2057 858 46
EaeI y'GGCC_r
Cuts at: 1627 2801 2975 3366 3393 4738 1627
Size: 1174 174 391 27 1345 2105
Fragments arranged by size:
2105 1345 1174 391 174 27
EagI C'GGCC_G
Cuts at: 2801 2801
Size: 5216
EarI CTCTTCn'nnn_
Cuts at: 1955 3239 3449 1955
Size: 1284 210 3722
Fragments arranged by size:
3722 1284 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3424 3734 3880 5026 3424
Size: 310 146 1146 3614
Fragments arranged by size:
3614 1146 310 146
EcoICRI GAG'CTC
Cuts at: 743 1398 2228 743
Size: 655 830 3731
Fragments arranged by size:
3731 830 655
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 292 358 666 721 1130 1377 1481 1534
Size: 102 66 308 55 409 247 104 53
Cuts at: 1534 1844 2014 3041 3473 4220 4488 190
Size: 310 170 1027 432 747 268 918
Fragments arranged by size:
1027 918 747 432 409 310 308 268
247 170 104 102 66 55 53
EcoO109I rG'GnC_Cy
Cuts at: 2067 2355 2067
Size: 288 4928
EcoRI G'AATT_C
Cuts at: 2220 2220
Size: 5216
EcoRV GAT'ATC
Cuts at: 1866 1866
Size: 5216
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2043 3235 2043
Size: 1192 4024
FspI TGC'GCA
Cuts at: 499 1835 2997 499
Size: 1336 1162 2718
Fragments arranged by size:
2718 1336 1162
HaeII r_GCGC'y
Cuts at: 238 437 925 1613 2650 2898 3920 4899 238
Size: 199 488 688 1037 248 1022 979 555
Fragments arranged by size:
1037 1022 979 688 555 488 248 199
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 571 603 1017 1049 1205 1237 1337 1369 3058
Size: 32 414 32 156 32 100 32 1689
Cuts at: 3058 3088 3090 3120 571
Size: 30 2 30 2667
Fragments arranged by size:
2667 1689 414 156 100 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 190 1778 2255 190
Size: 1588 477 3151
Fragments arranged by size:
3151 1588 477
KasI G'GCGC_C
Cuts at: 234 2646 2894 234
Size: 2412 248 2556
Fragments arranged by size:
2556 2412 248
KpnI G_GTAC'C
Cuts at: 2236 2236
Size: 5216
MluI A'CGCG_T
Cuts at: 4649 4649
Size: 5216
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 422 428 2223 3887 4071 422
Size: 6 1795 1664 184 1567
Fragments arranged by size:
1795 1664 1567 184 6
MscI TGG'CCA
Cuts at: 2977 2977
Size: 5216
MslI CAynn'nnrTG
Cuts at: 2035 3205 3332 2035
Size: 1170 127 3919
Fragments arranged by size:
3919 1170 127
MspA1I CmG'CkG
Cuts at: 1108 1203 2327 2641 3001 4014 4259 1108
Size: 95 1124 314 360 1013 245 2065
Fragments arranged by size:
2065 1124 1013 360 314 245 95
NaeI GCC'GGC
Cuts at: 3397 3397
Size: 5216
NarI GG'CG_CC
Cuts at: 235 2647 2895 235
Size: 2412 248 2556
Fragments arranged by size:
2556 2412 248
NcoI C'CATG_G
Cuts at: 1764 1764
Size: 5216
NgoMIV G'CCGG_C
Cuts at: 3395 3395
Size: 5216
NruI TCG'CGA
Cuts at: 1952 1952
Size: 5216
NspI r_CATG'y
Cuts at: 1593 1656 2269 2769 3676 1593
Size: 63 613 500 907 3133
Fragments arranged by size:
3133 907 613 500 63
PasI CC'CwG_GG
Cuts at: 1983 1983
Size: 5216
PciI A'CATG_T
Cuts at: 1589 3672 1589
Size: 2083 3133
PflMI CCAn_nnn'nTGG
Cuts at: 315 315
Size: 5216
PmeI GTTT'AAAC
Cuts at: 2215 2215
Size: 5216
PmlI CAC'GTG
Cuts at: 381 381
Size: 5216
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1954 1986 4355 4387 1954
Size: 32 2369 32 2783
Fragments arranged by size:
2783 2369 32 32
PpuMI rG'GwC_Cy
Cuts at: 2067 2067
Size: 5216
PshAI GACnn'nnGTC
Cuts at: 1409 1409
Size: 5216
PspOMI G'GGCC_C
Cuts at: 265 265
Size: 5216
PspXI vC'TCGA_Gb
Cuts at: 1072 1072
Size: 5216
PstI C_TGCA'G
Cuts at: 370 598 1698 2263 2948 370
Size: 228 1100 565 685 2638
Fragments arranged by size:
2638 1100 685 565 228
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5216
PvuII CAG'CTG
Cuts at: 1108 1203 2641 3001 1108
Size: 95 1438 360 3323
Fragments arranged by size:
3323 1438 360 95
RsrII CG'GwC_CG
Cuts at: 3411 3411
Size: 5216
SacI G_AGCT'C
Cuts at: 745 1400 2230 745
Size: 655 830 3731
Fragments arranged by size:
3731 830 655
SalI G'TCGA_C
Cuts at: 188 2253 188
Size: 2065 3151
SapI GCTCTTCn'nnn_
Cuts at: 3239 3449 3239
Size: 210 5006
SbfI CC_TGCA'GG
Cuts at: 2263 2263
Size: 5216
ScaI AGT'ACT
Cuts at: 4733 4733
Size: 5216
SfcI C'TryA_G
Cuts at: 33 366 546 594 1694 2259 2944 3937 4128
Size: 333 180 48 1100 565 685 993 191
Cuts at: 4128 33
Size: 1121
Fragments arranged by size:
1121 1100 993 685 565 333 191 180
48
SfoI GGC'GCC
Cuts at: 236 2648 2896 236
Size: 2412 248 2556
Fragments arranged by size:
2556 2412 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5216
SmaI CCC'GGG
Cuts at: 2238 2238
Size: 5216
SmlI C'TyrA_G
Cuts at: 229 738 1072 2371 3286 3778 4040 229
Size: 509 334 1299 915 492 262 1405
Fragments arranged by size:
1405 1299 915 509 492 334 262
SnaBI TAC'GTA
Cuts at: 2208 2208
Size: 5216
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5216
SphI G_CATG'C
Cuts at: 2269 2769 2269
Size: 500 4716
SspI AAT'ATT
Cuts at: 818 818
Size: 5216
StyI C'CwwG_G
Cuts at: 1424 1764 2350 2704 1424
Size: 340 586 354 3936
Fragments arranged by size:
3936 586 354 340
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1405 2799 2932 3391 3600 1405
Size: 1394 133 459 209 3021
Fragments arranged by size:
3021 1394 459 209 133
TatI w'GTAC_w
Cuts at: 4731 4731
Size: 5216
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1229 1261 1273 1305 4733 4765 1229
Size: 32 12 32 3428 32 1680
Fragments arranged by size:
3428 1680 32 32 32 12
Tth111I GACn'n_nGTC
Cuts at: 3013 3013
Size: 5216
XbaI T'CTAG_A
Cuts at: 1297 1537 2247 1297
Size: 240 710 4266
Fragments arranged by size:
4266 710 240
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 696 2711 696
Size: 2015 3201
XhoI C'TCGA_G
Cuts at: 1072 1072
Size: 5216
XmaI C'CCGG_G
Cuts at: 2236 2236
Size: 5216
XmnI GAAnn'nnTTC
Cuts at: 1999 1999
Size: 5216
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BfrBI BglI BmtI BsaXI BsgI
BsiWI BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NsiI PacI PfoI PsiI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI StuI SwaI ZraI