Restriction map for
pF1KSDA1325
(Circular) (Six-base) MAPSORT of: pF1KSDA1325.seq Check: 6774 from: 1 to: 4178
pF1KSDA1325 4178 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 1216 1216
Size: 4178
Acc65I G'GTAC_C
Cuts at: 1194 1194
Size: 4178
AclI AA'CG_TT
Cuts at: 4018 4018
Size: 4178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1030 2003 2435 3182 3450 1030
Size: 973 432 747 268 1758
Fragments arranged by size:
1758 973 747 432 268
AflII C'TTAA_G
Cuts at: 825 825
Size: 4178
AflIII A'CryG_T
Cuts at: 1530 2634 3611 1530
Size: 1104 977 2097
Fragments arranged by size:
2097 1104 977
AgeI A'CCGG_T
Cuts at: 2566 2566
Size: 4178
AlwNI CAG_nnn'CTG
Cuts at: 1483 3050 1483
Size: 1567 2611
ApaLI G'TGCA_C
Cuts at: 758 2948 758
Size: 2190 1988
ApoI r'AATT_y
Cuts at: 202 482 520 895 1163 1182 3531 3787 202
Size: 280 38 375 268 19 2349 256 593
Fragments arranged by size:
2349 593 375 280 268 256 38 19
AseI AT'TA_AT
Cuts at: 20 20
Size: 4178
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
AvaI C'yCGr_G
Cuts at: 661 1198 661
Size: 537 3641
BamHI G'GATC_C
Cuts at: 1203 1203
Size: 4178
BanI G'GyrC_C
Cuts at: 1194 1608 1856 1891 1194
Size: 414 248 35 3481
Fragments arranged by size:
3481 414 248 35
BanII G_rGCy'C
Cuts at: 689 770 1192 2222 689
Size: 81 422 1030 2645
Fragments arranged by size:
2645 1030 422 81
BbeI G_GCGC'C
Cuts at: 1612 1860 1612
Size: 248 3930
BbsI GAAGACnn'nnnn_
Cuts at: 1036 1036
Size: 4178
BciVI GTATCCnnnnn_n'
Cuts at: 795 2076 2837 4178 795
Size: 1281 761 1341 795
Fragments arranged by size:
1341 1281 795 761
BclI T'GATC_A
Cuts at: 1698 1698
Size: 4178
BglII A'GATC_T
Cuts at: 1693 3730 1693
Size: 2037 2141
BlpI GC'TnA_GC
Cuts at: 1290 1290
Size: 4178
Bme1580I G_kGCm'C
Cuts at: 762 1803 1896 2952 762
Size: 1041 93 1056 1988
Fragments arranged by size:
1988 1056 1041 93
BmrI ACTGGGnnnn_n'
Cuts at: 1390 1471 1806 3950 1390
Size: 81 335 2144 1618
Fragments arranged by size:
2144 1618 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2545 2545
Size: 4178
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 766 766
Size: 4178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 845 1354 2233 2725 3023 845
Size: 509 879 492 298 2000
Fragments arranged by size:
2000 879 509 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3882 3882
Size: 4178
BsaAI yAC'GTr
Cuts at: 1170 1531 1170
Size: 361 3817
BsaBI GATnn'nnATC
Cuts at: 6 1692 3729 6
Size: 1686 2037 455
Fragments arranged by size:
2037 1686 455
BsaHI Gr'CG_yC
Cuts at: 1609 1857 2559 4055 1609
Size: 248 702 1496 1732
Fragments arranged by size:
1732 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1367 1888 2566 2840 2987 3398 1367
Size: 521 678 274 147 411 2147
Fragments arranged by size:
2147 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 435 1603 2361 2938 3434 435
Size: 1168 758 577 496 1179
Fragments arranged by size:
1179 1168 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1720 1766 2974 104
Size: 1616 46 1208 1308
Fragments arranged by size:
1616 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 762 1192 1970 2160 2952 762
Size: 430 778 190 792 1988
Fragments arranged by size:
1988 792 778 430 190
BsmI GAATG_Cn'
Cuts at: 717 717
Size: 4178
Bsp1286I G_dGCh'C
Cuts at: 689 762 770 1192 1803 1896 1970 2160 2222
Size: 73 8 422 611 93 74 190 62
Cuts at: 2222 2952 689
Size: 730 1915
Fragments arranged by size:
1915 730 611 422 190 93 74 73
62 8
BspEI T'CCGG_A
Cuts at: 3398 3398
Size: 4178
BspHI T'CATG_A
Cuts at: 3354 3354
Size: 4178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1228 1442 1744 2125 1228
Size: 214 302 381 3281
Fragments arranged by size:
3281 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2470 2524 4008 4173 50
Size: 2420 54 1484 165 55
Fragments arranged by size:
2420 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 262 741 1022 2090 262
Size: 479 281 1068 2350
Fragments arranged by size:
2350 1068 479 281
BsrFI r'CCGG_y
Cuts at: 2176 2357 2566 2176
Size: 181 209 3788
Fragments arranged by size:
3788 209 181
BssSI C'ACGA_G
Cuts at: 871 2449 2807 871
Size: 1578 358 2242
Fragments arranged by size:
2242 1578 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1555 1555
Size: 4178
BstBI TT'CG_AA
Cuts at: 110 2539 110
Size: 2429 1749
BstYI r'GATC_y
Cuts at: 1 1203 1670 1693 2028 2274 3286 3372 3384
Size: 1202 467 23 335 246 1012 86 12
Cuts at: 3384 3730 1
Size: 346 449
Fragments arranged by size:
1202 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2128 2309 2472 2128
Size: 181 163 3834
Fragments arranged by size:
3834 181 163
BtsI GCAGTG_nn'
Cuts at: 826 1421 826
Size: 595 3583
DraI TTT'AAA
Cuts at: 1177 1177
Size: 4178
DrdI GACnn_nn'nnGTC
Cuts at: 1884 2742 1884
Size: 858 3320
EaeI y'GGCC_r
Cuts at: 1763 1937 2328 2355 3700 1763
Size: 174 391 27 1345 2241
Fragments arranged by size:
2241 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1763 1763
Size: 4178
EarI CTCTTCn'nnn_
Cuts at: 332 540 2201 2411 332
Size: 208 1661 210 2099
Fragments arranged by size:
2099 1661 210 208
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2386 2696 2842 3988 2386
Size: 310 146 1146 2576
Fragments arranged by size:
2576 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1190 1190
Size: 4178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 766 1030 2003 2435 3182 3450 766
Size: 264 973 432 747 268 1494
Fragments arranged by size:
1494 973 747 432 268 264
EcoO109I rG'GnC_Cy
Cuts at: 1317 1317
Size: 4178
EcoRI G'AATT_C
Cuts at: 1182 1182
Size: 4178
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 177 2197 177
Size: 2020 2158
FspI TGC'GCA
Cuts at: 1959 1959
Size: 4178
HaeII r_GCGC'y
Cuts at: 1612 1860 2882 3861 1612
Size: 248 1022 979 1929
Fragments arranged by size:
1929 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 776 808 2020 2050 2052 2082 776
Size: 32 1212 30 2 30 2872
Fragments arranged by size:
2872 1212 32 30 30 2
HincII GTy'rAC
Cuts at: 134 325 1217 134
Size: 191 892 3095
Fragments arranged by size:
3095 892 191
HindIII A'AGCT_T
Cuts at: 1103 1103
Size: 4178
KasI G'GCGC_C
Cuts at: 1608 1856 1608
Size: 248 3930
KpnI G_GTAC'C
Cuts at: 1198 1198
Size: 4178
MluI A'CGCG_T
Cuts at: 3611 3611
Size: 4178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2849 3033 2849
Size: 184 3994
MscI TGG'CCA
Cuts at: 1939 1939
Size: 4178
MslI CAynn'nnrTG
Cuts at: 961 1015 2167 2294 961
Size: 54 1152 127 2845
Fragments arranged by size:
2845 1152 127 54
MspA1I CmG'CkG
Cuts at: 529 1289 1603 1963 2976 3221 529
Size: 760 314 360 1013 245 1486
Fragments arranged by size:
1486 1013 760 360 314 245
NaeI GCC'GGC
Cuts at: 2359 2359
Size: 4178
NarI GG'CG_CC
Cuts at: 1609 1857 1609
Size: 248 3930
NgoMIV G'CCGG_C
Cuts at: 2357 2357
Size: 4178
NspI r_CATG'y
Cuts at: 731 1231 1731 2638 731
Size: 500 500 907 2271
Fragments arranged by size:
2271 907 500 500
PasI CC'CwG_GG
Cuts at: 753 753
Size: 4178
PciI A'CATG_T
Cuts at: 2634 2634
Size: 4178
PfoI T'CCnGG_A
Cuts at: 989 989
Size: 4178
PmeI GTTT'AAAC
Cuts at: 1177 1177
Size: 4178
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3317 3349 3317
Size: 32 4146
PstI C_TGCA'G
Cuts at: 821 1225 1910 821
Size: 404 685 3089
Fragments arranged by size:
3089 685 404
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4178
PvuII CAG'CTG
Cuts at: 529 1603 1963 529
Size: 1074 360 2744
Fragments arranged by size:
2744 1074 360
RsrII CG'GwC_CG
Cuts at: 2373 2373
Size: 4178
SacI G_AGCT'C
Cuts at: 1192 1192
Size: 4178
SalI G'TCGA_C
Cuts at: 1215 1215
Size: 4178
SapI GCTCTTCn'nnn_
Cuts at: 540 2201 2411 540
Size: 1661 210 2307
Fragments arranged by size:
2307 1661 210
SbfI CC_TGCA'GG
Cuts at: 1225 1225
Size: 4178
ScaI AGT'ACT
Cuts at: 3695 3695
Size: 4178
SfcI C'TryA_G
Cuts at: 33 817 1221 1906 2899 3090 33
Size: 784 404 685 993 191 1121
Fragments arranged by size:
1121 993 784 685 404 191
SfoI GGC'GCC
Cuts at: 1610 1858 1610
Size: 248 3930
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4178
SmaI CCC'GGG
Cuts at: 1200 1200
Size: 4178
SmlI C'TyrA_G
Cuts at: 825 860 1333 2248 2740 3002 825
Size: 35 473 915 492 262 2001
Fragments arranged by size:
2001 915 492 473 262 35
SnaBI TAC'GTA
Cuts at: 1170 1170
Size: 4178
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4178
SphI G_CATG'C
Cuts at: 1231 1731 1231
Size: 500 3678
StyI C'CwwG_G
Cuts at: 680 689 1312 1666 680
Size: 9 623 354 3192
Fragments arranged by size:
3192 623 354 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 253 1761 1894 2353 2562 253
Size: 1508 133 459 209 1869
Fragments arranged by size:
1869 1508 459 209 133
TatI w'GTAC_w
Cuts at: 422 773 3693 422
Size: 351 2920 907
Fragments arranged by size:
2920 907 351
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3695 3727 3695
Size: 32 4146
Tth111I GACn'n_nGTC
Cuts at: 1975 1975
Size: 4178
XbaI T'CTAG_A
Cuts at: 447 1209 447
Size: 762 3416
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 760 1673 760
Size: 913 3265
XmaI C'CCGG_G
Cuts at: 1198 1198
Size: 4178
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PflMI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI SspI StuI SwaI XhoI
XmnI ZraI