Restriction map for
pF1KSDA1334
(Circular) (Six-base) MAPSORT of: pF1KSDA1334.seq Check: 1556 from: 1 to: 6077
pF1KSDA1334 6077 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 3115 3115
Size: 6077
Acc65I G'GTAC_C
Cuts at: 2475 3093 2475
Size: 618 5459
AclI AA'CG_TT
Cuts at: 5917 5917
Size: 6077
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 1169 1490 3043 3902 4334 5081 5349 427
Size: 742 321 1553 859 432 747 268 1155
Fragments arranged by size:
1553 1155 859 747 742 432 321 268
AflII C'TTAA_G
Cuts at: 2386 2386
Size: 6077
AflIII A'CryG_T
Cuts at: 318 2192 3429 4533 5510 318
Size: 1874 1237 1104 977 885
Fragments arranged by size:
1874 1237 1104 977 885
AgeI A'CCGG_T
Cuts at: 4465 4465
Size: 6077
AleI CACnn'nnGTG
Cuts at: 275 275
Size: 6077
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2812 2844 2812
Size: 32 6045
AlwNI CAG_nnn'CTG
Cuts at: 1123 1425 1740 2877 3382 4949 1123
Size: 302 315 1137 505 1567 2251
Fragments arranged by size:
2251 1567 1137 505 315 302
ApaI G_GGCC'C
Cuts at: 1312 1312
Size: 6077
ApaLI G'TGCA_C
Cuts at: 4847 4847
Size: 6077
ApoI r'AATT_y
Cuts at: 821 1415 2689 3081 5430 5686 821
Size: 594 1274 392 2349 256 1212
Fragments arranged by size:
2349 1274 1212 594 392 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6077
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6077
AvaI C'yCGr_G
Cuts at: 3097 3097
Size: 6077
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2467 2500 2467
Size: 33 6044
BamHI G'GATC_C
Cuts at: 3102 3102
Size: 6077
BanI G'GyrC_C
Cuts at: 2475 3093 3507 3755 3790 2475
Size: 618 414 248 35 4762
Fragments arranged by size:
4762 618 414 248 35
BanII G_rGCy'C
Cuts at: 556 1312 1468 2743 3091 4121 556
Size: 756 156 1275 348 1030 2512
Fragments arranged by size:
2512 1275 1030 756 348 156
BbeI G_GCGC'C
Cuts at: 3511 3759 3511
Size: 248 5829
BbsI GAAGACnn'nnnn_
Cuts at: 2477 2477
Size: 6077
BbvCI CC'TCA_GC
Cuts at: 1567 1699 2006 1567
Size: 132 307 5638
Fragments arranged by size:
5638 307 132
BciVI GTATCCnnnnn_n'
Cuts at: 772 3975 4736 6077 772
Size: 3203 761 1341 772
Fragments arranged by size:
3203 1341 772 761
BclI T'GATC_A
Cuts at: 3597 3597
Size: 6077
BfrBI ATG'CAT
Cuts at: 428 1750 2970 428
Size: 1322 1220 3535
Fragments arranged by size:
3535 1322 1220
BglII A'GATC_T
Cuts at: 623 1164 1443 3592 5629 623
Size: 541 279 2149 2037 1071
Fragments arranged by size:
2149 2037 1071 541 279
BlpI GC'TnA_GC
Cuts at: 2096 3189 2096
Size: 1093 4984
Bme1580I G_kGCm'C
Cuts at: 1312 3702 3795 4851 1312
Size: 2390 93 1056 2538
Fragments arranged by size:
2538 2390 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 969 3289 3370 3705 5849 969
Size: 2320 81 335 2144 1197
Fragments arranged by size:
2320 2144 1197 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1575 2141 4444 1575
Size: 566 2303 3208
Fragments arranged by size:
3208 2303 566
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2122 2449 2485 2773 2893 2899 2122
Size: 327 36 288 120 6 5300
Fragments arranged by size:
5300 327 288 120 36 6
Bpu10I CC'TnA_GC
Cuts at: 509 1270 1567 1699 2006 2702 509
Size: 761 297 132 307 696 3884
Fragments arranged by size:
3884 761 696 307 297 132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3253 4132 4624 4922 3253
Size: 879 492 298 4408
Fragments arranged by size:
4408 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2613 5781 2613
Size: 3168 2909
BsaAI yAC'GTr
Cuts at: 321 3069 3430 321
Size: 2748 361 2968
Fragments arranged by size:
2968 2748 361
BsaBI GATnn'nnATC
Cuts at: 6 3591 5628 6
Size: 3585 2037 455
Fragments arranged by size:
3585 2037 455
BsaHI Gr'CG_yC
Cuts at: 3508 3756 4458 5954 3508
Size: 248 702 1496 3631
Fragments arranged by size:
3631 1496 702 248
BsaWI w'CCGG_w
Cuts at: 379 3266 3787 4465 4739 4886 5297 379
Size: 2887 521 678 274 147 411 1159
Fragments arranged by size:
2887 1159 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 641 671 999 1029 2812 2842 641
Size: 30 328 30 1783 30 3876
Fragments arranged by size:
3876 1783 328 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1618 1618
Size: 6077
BseYI C'CCAG_C
Cuts at: 456 2267 2831 3502 4260 4837 5333 456
Size: 1811 564 671 758 577 496 1200
Fragments arranged by size:
1811 1200 758 671 577 564 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 770 1651 3007 550
Size: 220 881 1356 3620
Fragments arranged by size:
3620 1356 881 220
BsiEI CG_ry'CG
Cuts at: 104 3619 3665 4873 104
Size: 3515 46 1208 1308
Fragments arranged by size:
3515 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1468 2743 3091 3869 4059 4851 1468
Size: 1275 348 778 190 792 2694
Fragments arranged by size:
2694 1275 792 778 348 190
BsmI GAATG_Cn'
Cuts at: 331 430 1752 2932 2972 331
Size: 99 1322 1180 40 3436
Fragments arranged by size:
3436 1322 1180 99 40
Bsp1286I G_dGCh'C
Cuts at: 556 1312 1468 2743 3091 3702 3795 3869 4059
Size: 756 156 1275 348 611 93 74 190
Cuts at: 4059 4121 4851 556
Size: 62 730 1782
Fragments arranged by size:
1782 1275 756 730 611 348 190 156
93 74 62
BspEI T'CCGG_A
Cuts at: 5297 5297
Size: 6077
BspHI T'CATG_A
Cuts at: 338 1949 5253 338
Size: 1611 3304 1162
Fragments arranged by size:
3304 1611 1162
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3127 3341 3643 4024 3127
Size: 214 302 381 5180
Fragments arranged by size:
5180 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4369 4423 5907 6072 50
Size: 4319 54 1484 165 55
Fragments arranged by size:
4319 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2477 3989 2477
Size: 1512 4565
BsrFI r'CCGG_y
Cuts at: 188 4075 4256 4465 188
Size: 3887 181 209 1800
Fragments arranged by size:
3887 1800 209 181
BsrGI T'GTAC_A
Cuts at: 603 2078 603
Size: 1475 4602
BssSI C'ACGA_G
Cuts at: 1693 4348 4706 1693
Size: 2655 358 3064
Fragments arranged by size:
3064 2655 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1192 1740 2215 3454 1192
Size: 548 475 1239 3815
Fragments arranged by size:
3815 1239 548 475
BstBI TT'CG_AA
Cuts at: 110 4438 110
Size: 4328 1749
BstYI r'GATC_y
Cuts at: 1 623 1164 1443 1737 3102 3569 3592 3927
Size: 622 541 279 294 1365 467 23 335
Cuts at: 3927 4173 5185 5271 5283 5629 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1365 1012 622 541 467 449 346 335
294 279 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 331 331
Size: 6077
BtgI C'CryG_G
Cuts at: 203 203
Size: 6077
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4027 4208 4371 4027
Size: 181 163 5733
Fragments arranged by size:
5733 181 163
BtsI GCAGTG_nn'
Cuts at: 235 3320 235
Size: 3085 2992
DraI TTT'AAA
Cuts at: 866 3076 866
Size: 2210 3867
DraIII CAC_nnn'GTG
Cuts at: 352 352
Size: 6077
DrdI GACnn_nn'nnGTC
Cuts at: 1408 2193 3783 4641 1408
Size: 785 1590 858 2844
Fragments arranged by size:
2844 1590 858 785
EaeI y'GGCC_r
Cuts at: 3662 3836 4227 4254 5599 3662
Size: 174 391 27 1345 4140
Fragments arranged by size:
4140 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3662 3662
Size: 6077
EarI CTCTTCn'nnn_
Cuts at: 124 148 996 1447 1706 1841 2110 2441 2748
Size: 24 848 451 259 135 269 331 307
Cuts at: 2748 4100 4310 124
Size: 1352 210 1891
Fragments arranged by size:
1891 1352 848 451 331 307 269 259
210 135 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1273 2938 4285 4595 4741 5887 1273
Size: 1665 1347 310 146 1146 1463
Fragments arranged by size:
1665 1463 1347 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1466 2741 3089 1466
Size: 1275 348 4454
Fragments arranged by size:
4454 1275 348
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 1169 1490 2122 2449 2485 2773 2893 2899
Size: 742 321 632 327 36 288 120 6
Cuts at: 2899 3043 3902 4334 5081 5349 427
Size: 144 859 432 747 268 1155
Fragments arranged by size:
1155 859 747 742 632 432 327 321
288 268 144 120 36 6
EcoO109I rG'GnC_Cy
Cuts at: 3216 3216
Size: 6077
EcoRI G'AATT_C
Cuts at: 821 1415 3081 821
Size: 594 1666 3817
Fragments arranged by size:
3817 1666 594
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1639 2832 4096 1639
Size: 1193 1264 3620
Fragments arranged by size:
3620 1264 1193
FspI TGC'GCA
Cuts at: 3858 3858
Size: 6077
HaeII r_GCGC'y
Cuts at: 392 2887 3511 3759 4781 5760 392
Size: 2495 624 248 1022 979 709
Fragments arranged by size:
2495 1022 979 709 624 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 641 673 886 918 1156 1188 1339 1371 2206
Size: 32 213 32 238 32 151 32 835
Cuts at: 2206 2238 2938 2970 3919 3949 3951 3981 641
Size: 32 700 32 949 30 2 30 2737
Fragments arranged by size:
2737 949 835 700 238 213 151 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 471 2264 3116 471
Size: 1793 852 3432
Fragments arranged by size:
3432 1793 852
HindIII A'AGCT_T
Cuts at: 1272 1272
Size: 6077
KasI G'GCGC_C
Cuts at: 3507 3755 3507
Size: 248 5829
KpnI G_GTAC'C
Cuts at: 2479 3097 2479
Size: 618 5459
MfeI C'AATT_G
Cuts at: 2182 2182
Size: 6077
MluI A'CGCG_T
Cuts at: 5510 5510
Size: 6077
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4748 4932 4748
Size: 184 5893
MscI TGG'CCA
Cuts at: 3838 3838
Size: 6077
MslI CAynn'nnrTG
Cuts at: 275 4066 4193 275
Size: 3791 127 2159
Fragments arranged by size:
3791 2159 127
MspA1I CmG'CkG
Cuts at: 505 591 2076 2143 2877 2919 3188 3502 3862
Size: 86 1485 67 734 42 269 314 360
Cuts at: 3862 4875 5120 505
Size: 1013 245 1462
Fragments arranged by size:
1485 1462 1013 734 360 314 269 245
86 67 42
NaeI GCC'GGC
Cuts at: 4258 4258
Size: 6077
NarI GG'CG_CC
Cuts at: 3508 3756 3508
Size: 248 5829
NgoMIV G'CCGG_C
Cuts at: 4256 4256
Size: 6077
NsiI A_TGCA'T
Cuts at: 430 1752 2972 430
Size: 1322 1220 3535
Fragments arranged by size:
3535 1322 1220
NspI r_CATG'y
Cuts at: 3130 3630 4537 3130
Size: 500 907 4670
Fragments arranged by size:
4670 907 500
PacI TTA_AT'TAA
Cuts at: 740 740
Size: 6077
PasI CC'CwG_GG
Cuts at: 1317 1317
Size: 6077
PciI A'CATG_T
Cuts at: 4533 4533
Size: 6077
PflMI CCAn_nnn'nTGG
Cuts at: 170 170
Size: 6077
PfoI T'CCnGG_A
Cuts at: 638 664 638
Size: 26 6051
PmeI GTTT'AAAC
Cuts at: 3076 3076
Size: 6077
PmlI CAC'GTG
Cuts at: 321 321
Size: 6077
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2293 2325 2812 2844 5216 5248 2293
Size: 32 487 32 2372 32 3122
Fragments arranged by size:
3122 2372 487 32 32 32
PspOMI G'GGCC_C
Cuts at: 1308 1308
Size: 6077
PstI C_TGCA'G
Cuts at: 200 1745 2355 2891 3124 3809 200
Size: 1545 610 536 233 685 2468
Fragments arranged by size:
2468 1545 685 610 536 233
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6077
PvuII CAG'CTG
Cuts at: 2076 2143 2877 2919 3502 3862 2076
Size: 67 734 42 583 360 4291
Fragments arranged by size:
4291 734 583 360 67 42
RsrII CG'GwC_CG
Cuts at: 4272 4272
Size: 6077
SacI G_AGCT'C
Cuts at: 1468 2743 3091 1468
Size: 1275 348 4454
Fragments arranged by size:
4454 1275 348
SalI G'TCGA_C
Cuts at: 3114 3114
Size: 6077
SapI GCTCTTCn'nnn_
Cuts at: 124 2748 4100 4310 124
Size: 2624 1352 210 1891
Fragments arranged by size:
2624 1891 1352 210
SbfI CC_TGCA'GG
Cuts at: 3124 3124
Size: 6077
ScaI AGT'ACT
Cuts at: 1113 3020 5594 1113
Size: 1907 2574 1596
Fragments arranged by size:
2574 1907 1596
SexAI A'CCwGG_T
Cuts at: 1326 1326
Size: 6077
SfcI C'TryA_G
Cuts at: 33 196 1115 1447 1489 1741 2351 2887 3120
Size: 163 919 332 42 252 610 536 233
Cuts at: 3120 3805 4798 4989 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 919 685 610 536 332 252
233 191 163 42
SfoI GGC'GCC
Cuts at: 3509 3757 3509
Size: 248 5829
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6077
SmaI CCC'GGG
Cuts at: 3099 3099
Size: 6077
SmlI C'TyrA_G
Cuts at: 2386 3232 4147 4639 4901 2386
Size: 846 915 492 262 3562
Fragments arranged by size:
3562 915 846 492 262
SnaBI TAC'GTA
Cuts at: 3069 3069
Size: 6077
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6077
SphI G_CATG'C
Cuts at: 3130 3630 3130
Size: 500 5577
SspI AAT'ATT
Cuts at: 2637 2637
Size: 6077
StuI AGG'CCT
Cuts at: 2815 2815
Size: 6077
StyI C'CwwG_G
Cuts at: 1250 3211 3565 1250
Size: 1961 354 3762
Fragments arranged by size:
3762 1961 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1263 1309 1639 2471 3660 3793 4252 4461 1263
Size: 46 330 832 1189 133 459 209 2879
Fragments arranged by size:
2879 1189 832 459 330 209 133 46
TatI w'GTAC_w
Cuts at: 603 1111 2078 3018 5592 603
Size: 508 967 940 2574 1088
Fragments arranged by size:
2574 1088 967 940 508
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 660 692 5594 5626 660
Size: 32 4902 32 1111
Fragments arranged by size:
4902 1111 32 32
Tth111I GACn'n_nGTC
Cuts at: 898 3874 898
Size: 2976 3101
XbaI T'CTAG_A
Cuts at: 2127 3108 2127
Size: 981 5096
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3572 3572
Size: 6077
XmaI C'CCGG_G
Cuts at: 3097 3097
Size: 6077
XmnI GAAnn'nnTTC
Cuts at: 2636 2636
Size: 6077
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AscI AvrII BcgI
BglI BmgBI BmtI BsiWI BsmBI BssHII BstEII BstXI
BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI HpaI
NcoI NdeI NheI NotI NruI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SfiI SgrAI SrfI SwaI
XhoI ZraI