Restriction map for
pF1KSDA1337
(Circular) (Six-base) MAPSORT of: pF1KSDA1337.seq Check: 5090 from: 1 to: 7313
pF1KSDA1337 7313 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3189 3189
Size: 7313
AatII G_ACGT'C
Cuts at: 2536 2536
Size: 7313
AccI GT'mk_AC
Cuts at: 1435 1556 2657 2791 3239 3499 4351 1435
Size: 121 1101 134 448 260 852 4397
Fragments arranged by size:
4397 1101 852 448 260 134 121
Acc65I G'GTAC_C
Cuts at: 818 4329 818
Size: 3511 3802
AclI AA'CG_TT
Cuts at: 7153 7153
Size: 7313
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 1315 2868 3335 5138 5570 6317 6585 553
Size: 762 1553 467 1803 432 747 268 1281
Fragments arranged by size:
1803 1553 1281 762 747 467 432 268
AflII C'TTAA_G
Cuts at: 502 502
Size: 7313
AflIII A'CryG_T
Cuts at: 2063 2183 4665 5769 6746 2063
Size: 120 2482 1104 977 2630
Fragments arranged by size:
2630 2482 1104 977 120
AgeI A'CCGG_T
Cuts at: 5701 5701
Size: 7313
AleI CACnn'nnGTG
Cuts at: 1411 4105 1411
Size: 2694 4619
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2851 3421 4159 2851
Size: 570 738 6005
Fragments arranged by size:
6005 738 570
AlwNI CAG_nnn'CTG
Cuts at: 600 801 1206 1538 2271 2430 3345 3576 4618
Size: 201 405 332 733 159 915 231 1042
Cuts at: 4618 6185 600
Size: 1567 1728
Fragments arranged by size:
1728 1567 1042 915 733 405 332 231
201 159
ApaI G_GGCC'C
Cuts at: 219 233 2110 2394 3339 4230 219
Size: 14 1877 284 945 891 3302
Fragments arranged by size:
3302 1877 945 891 284 14
ApaLI G'TGCA_C
Cuts at: 6083 6083
Size: 7313
ApoI r'AATT_y
Cuts at: 4317 6666 6922 4317
Size: 2349 256 4708
Fragments arranged by size:
4708 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7313
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7313
AvaI C'yCGr_G
Cuts at: 632 2077 2206 3225 3409 3426 3589 4333 632
Size: 1445 129 1019 184 17 163 744 3612
Fragments arranged by size:
3612 1445 1019 744 184 163 129 17
BamHI G'GATC_C
Cuts at: 517 4338 517
Size: 3821 3492
BanI G'GyrC_C
Cuts at: 818 880 1986 2611 2724 2979 3275 4158 4207
Size: 62 1106 625 113 255 296 883 49
Cuts at: 4207 4240 4329 4743 4991 5026 818
Size: 33 89 414 248 35 3105
Fragments arranged by size:
3105 1106 883 625 414 296 255 248
113 89 62 49 35 33
BanII G_rGCy'C
Cuts at: 219 233 639 956 1111 1225 1303 2002 2110
Size: 14 406 317 155 114 78 699 108
Cuts at: 2110 2394 2821 3339 3416 3598 4230 4327 5357
Size: 284 427 518 77 182 632 97 1030
Cuts at: 5357 219
Size: 2175
Fragments arranged by size:
2175 1030 699 632 518 427 406 317
284 182 155 114 108 97 78 77
14
BbeI G_GCGC'C
Cuts at: 884 4162 4747 4995 884
Size: 3278 585 248 3202
Fragments arranged by size:
3278 3202 585 248
BbsI GAAGACnn'nnnn_
Cuts at: 788 1775 1857 2044 3159 788
Size: 987 82 187 1115 4942
Fragments arranged by size:
4942 1115 987 187 82
BbvCI CC'TCA_GC
Cuts at: 3763 3763
Size: 7313
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3282 3316 3282
Size: 34 7279
BciVI GTATCCnnnnn_n'
Cuts at: 1669 3135 5211 5972 7313 1669
Size: 1466 2076 761 1341 1669
Fragments arranged by size:
2076 1669 1466 1341 761
BclI T'GATC_A
Cuts at: 4833 4833
Size: 7313
BfrBI ATG'CAT
Cuts at: 2975 2975
Size: 7313
BglI GCCn_nnn'nGGC
Cuts at: 1423 2389 3718 1423
Size: 966 1329 5018
Fragments arranged by size:
5018 1329 966
BglII A'GATC_T
Cuts at: 1178 4828 6865 1178
Size: 3650 2037 1626
Fragments arranged by size:
3650 2037 1626
BlpI GC'TnA_GC
Cuts at: 802 2998 4425 802
Size: 2196 1427 3690
Fragments arranged by size:
3690 2196 1427
Bme1580I G_kGCm'C
Cuts at: 219 233 873 2110 2394 2729 3059 3278 3339
Size: 14 640 1237 284 335 330 219 61
Cuts at: 3339 4017 4043 4230 4938 5031 6087 219
Size: 678 26 187 708 93 1056 1445
Fragments arranged by size:
1445 1237 1056 708 678 640 335 330
284 219 187 93 61 26 14
BmgBI CAC'GTC
Cuts at: 2064 2184 3213 2064
Size: 120 1029 6164
Fragments arranged by size:
6164 1029 120
BmrI ACTGGGnnnn_n'
Cuts at: 504 2292 2934 3333 4525 4606 4941 7085 504
Size: 1788 642 399 1192 81 335 2144 732
Fragments arranged by size:
2144 1788 1192 732 642 399 335 81
BmtI G_CTAG'C
Cuts at: 2579 2579
Size: 7313
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1296 2484 2745 5680 1296
Size: 1188 261 2935 2929
Fragments arranged by size:
2935 2929 1188 261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 327 775 1249 1992 2014 2164 2191 2560 2791
Size: 448 474 743 22 150 27 369 231
Cuts at: 2791 2988 3550 3828 3932 3993 3994 327
Size: 197 562 278 104 61 1 3646
Fragments arranged by size:
3646 743 562 474 448 369 278 231
197 150 104 61 27 22 1
Bpu10I CC'TnA_GC
Cuts at: 2452 2559 3763 2452
Size: 107 1204 6002
Fragments arranged by size:
6002 1204 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1075 2425 4180 4489 5368 5860 6158 1075
Size: 1350 1755 309 879 492 298 2230
Fragments arranged by size:
2230 1755 1350 879 492 309 298
BsaI GGTCTCn'nnnn_
Cuts at: 736 1618 3215 7017 736
Size: 882 1597 3802 1032
Fragments arranged by size:
3802 1597 1032 882
BsaAI yAC'GTr
Cuts at: 1413 1647 2895 3506 3990 4305 4666 1413
Size: 234 1248 611 484 315 361 4060
Fragments arranged by size:
4060 1248 611 484 361 315 234
BsaBI GATnn'nnATC
Cuts at: 6 4827 6864 6
Size: 4821 2037 455
Fragments arranged by size:
4821 2037 455
BsaHI Gr'CG_yC
Cuts at: 881 2533 3182 3455 3944 4159 4744 4992 5694
Size: 1652 649 273 489 215 585 248 702
Cuts at: 5694 7190 881
Size: 1496 1004
Fragments arranged by size:
1652 1496 1004 702 649 585 489 273
248 215
BsaWI w'CCGG_w
Cuts at: 2528 4502 5023 5701 5975 6122 6533 2528
Size: 1974 521 678 274 147 411 3308
Fragments arranged by size:
3308 1974 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 541 571 1852 1882 2650 2680 3349 3379 541
Size: 30 1281 30 768 30 669 30 4475
Fragments arranged by size:
4475 1281 768 669 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 184 187 196 3357 3751 3855 184
Size: 3 9 3161 394 104 3642
Fragments arranged by size:
3642 3161 394 104 9 3
BseYI C'CCAG_C
Cuts at: 291 320 346 826 890 1104 1132 1367 1878
Size: 29 26 480 64 214 28 235 511
Cuts at: 1878 2175 2312 3551 3815 4146 4162 4236 4738
Size: 297 137 1239 264 331 16 74 502
Cuts at: 4738 5496 6073 6569 291
Size: 758 577 496 1035
Fragments arranged by size:
1239 1035 758 577 511 502 496 480
331 297 264 235 214 137 74 64
29 28 26 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2032 2260 2278 3493 3685 2032
Size: 228 18 1215 192 5660
Fragments arranged by size:
5660 1215 228 192 18
BsiEI CG_ry'CG
Cuts at: 104 4855 4901 6109 104
Size: 4751 46 1208 1308
Fragments arranged by size:
4751 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 359 956 1091 1303 1335 2002 3632 3761 4256
Size: 597 135 212 32 667 1630 129 495
Cuts at: 4256 4327 5105 5295 6087 359
Size: 71 778 190 792 1585
Fragments arranged by size:
1630 1585 792 778 667 597 495 212
190 135 129 71 32
BsiWI C'GTAC_G
Cuts at: 3967 3967
Size: 7313
BsmBI CGTCTCn'nnnn_
Cuts at: 556 2054 2174 2340 3073 4011 556
Size: 1498 120 166 733 938 3858
Fragments arranged by size:
3858 1498 938 733 166 120
Bsp1286I G_dGCh'C
Cuts at: 219 233 359 639 873 956 1091 1111 1225
Size: 14 126 280 234 83 135 20 114
Cuts at: 1225 1303 1335 2002 2110 2394 2729 2821 3059
Size: 78 32 667 108 284 335 92 238
Cuts at: 3059 3278 3339 3416 3598 3632 3761 4017 4043
Size: 219 61 77 182 34 129 256 26
Cuts at: 4043 4230 4256 4327 4938 5031 5105 5295 5357
Size: 187 26 71 611 93 74 190 62
Cuts at: 5357 6087 219
Size: 730 1445
Fragments arranged by size:
1445 730 667 611 335 284 280 256
238 234 219 190 187 182 135 129
126 114 108 93 92 83 78 77
74 71 62 61 34 32 26 26
20 14
BspEI T'CCGG_A
Cuts at: 2528 6533 2528
Size: 4005 3308
BspHI T'CATG_A
Cuts at: 1142 6489 1142
Size: 5347 1966
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2073 3189 3428 3554 3631 3911 3929 4363 4577
Size: 1116 239 126 77 280 18 434 214
Cuts at: 4577 4879 5260 2073
Size: 302 381 4126
Fragments arranged by size:
4126 1116 434 381 302 280 239 214
126 77 18
BsrBI CCG'CTC
Cuts at: 50 617 725 3812 4265 5605 5659 7143 7308
Size: 567 108 3087 453 1340 54 1484 165
Cuts at: 7308 50
Size: 55
Fragments arranged by size:
3087 1484 1340 567 453 165 108 55
54
BsrDI GCAATG_nn'
Cuts at: 1541 5225 1541
Size: 3684 3629
BsrFI r'CCGG_y
Cuts at: 533 2369 2579 3120 5311 5492 5701 533
Size: 1836 210 541 2191 181 209 2145
Fragments arranged by size:
2191 2145 1836 541 210 209 181
BsrGI T'GTAC_A
Cuts at: 2618 2618
Size: 7313
BssHII G'CGCG_C
Cuts at: 903 967 903
Size: 64 7249
BssSI C'ACGA_G
Cuts at: 463 1081 4249 5584 5942 463
Size: 618 3168 1335 358 1834
Fragments arranged by size:
3168 1834 1335 618 358
BstAPI GCAn_nnn'nTGC
Cuts at: 600 1538 2271 2471 3384 3576 3585 3782 4690
Size: 938 733 200 913 192 9 197 908
Cuts at: 4690 600
Size: 3223
Fragments arranged by size:
3223 938 913 908 733 200 197 192
9
BstBI TT'CG_AA
Cuts at: 110 5674 110
Size: 5564 1749
BstEII G'GTnAC_C
Cuts at: 1404 3792 1404
Size: 2388 4925
BstXI CCAn_nnnn'nTGG
Cuts at: 1764 1894 3217 1764
Size: 130 1323 5860
Fragments arranged by size:
5860 1323 130
BstYI r'GATC_y
Cuts at: 1 452 517 759 1095 1178 1319 1874 3479
Size: 451 65 242 336 83 141 555 1605
Cuts at: 3479 4338 4805 4828 5163 5409 6421 6507 6519
Size: 859 467 23 335 246 1012 86 12
Cuts at: 6519 6865 1
Size: 346 449
Fragments arranged by size:
1605 1012 859 555 467 451 449 346
336 335 246 242 141 86 83 65
23 12
Bsu36I CC'TnA_GG
Cuts at: 2585 2585
Size: 7313
BtgI C'CryG_G
Cuts at: 125 380 653 695 2241 2961 3152 3441 3485
Size: 255 273 42 1546 720 191 289 44
Cuts at: 3485 3713 3869 4022 125
Size: 228 156 153 3416
Fragments arranged by size:
3416 1546 720 289 273 255 228 191
156 153 44 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3285 4014 4044 5263 5444 5607 3285
Size: 729 30 1219 181 163 4991
Fragments arranged by size:
4991 1219 729 181 163 30
BtsI GCAGTG_nn'
Cuts at: 1320 3455 3481 4556 1320
Size: 2135 26 1075 4077
Fragments arranged by size:
4077 2135 1075 26
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2008 2043 2008
Size: 35 7278
DraI TTT'AAA
Cuts at: 4312 4312
Size: 7313
DraIII CAC_nnn'GTG
Cuts at: 1086 1086
Size: 7313
DrdI GACnn_nn'nnGTC
Cuts at: 3211 5019 5877 3211
Size: 1808 858 4647
Fragments arranged by size:
4647 1808 858
EaeI y'GGCC_r
Cuts at: 545 854 1235 1415 1424 1688 1934 2244 2897
Size: 309 381 180 9 264 246 310 653
Cuts at: 2897 3130 3710 3719 3998 4898 5072 5463 5490
Size: 233 580 9 279 900 174 391 27
Cuts at: 5490 6835 545
Size: 1345 1023
Fragments arranged by size:
1345 1023 900 653 580 391 381 310
309 279 264 246 233 180 174 27
9 9
EagI C'GGCC_G
Cuts at: 4898 4898
Size: 7313
EarI CTCTTCn'nnn_
Cuts at: 364 1291 1639 2150 2407 2542 2629 3265 4051
Size: 927 348 511 257 135 87 636 786
Cuts at: 4051 5336 5546 364
Size: 1285 210 2131
Fragments arranged by size:
2131 1285 927 786 636 511 348 257
210 135 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 543 1225 2365 5521 5831 5977 7123 543
Size: 682 1140 3156 310 146 1146 733
Fragments arranged by size:
3156 1146 1140 733 682 310 146
EcoICRI GAG'CTC
Cuts at: 954 1301 2000 4325 954
Size: 347 699 2325 3942
Fragments arranged by size:
3942 2325 699 347
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 327 553 775 1249 1315 1992 2014 2164 2191
Size: 226 222 474 66 677 22 150 27
Cuts at: 2191 2560 2791 2868 2988 3335 3550 3828 3932
Size: 369 231 77 120 347 215 278 104
Cuts at: 3932 3993 3994 5138 5570 6317 6585 327
Size: 61 1 1144 432 747 268 1055
Fragments arranged by size:
1144 1055 747 677 474 432 369 347
278 268 231 226 222 215 150 120
104 77 66 61 27 22 1
EcoNI CCTnn'n_nnAGG
Cuts at: 242 3023 3262 3292 3892 242
Size: 2781 239 30 600 3663
Fragments arranged by size:
3663 2781 600 239 30
EcoO109I rG'GnC_Cy
Cuts at: 215 229 230 676 1203 1275 2106 2107 2286
Size: 14 1 446 527 72 831 1 179
Cuts at: 2286 2391 2565 3335 4200 4226 4227 4292 4452
Size: 105 174 770 865 26 1 65 160
Cuts at: 4452 215
Size: 3076
Fragments arranged by size:
3076 865 831 770 527 446 179 174
160 105 72 65 26 14 1 1
1
EcoRI G'AATT_C
Cuts at: 4317 4317
Size: 7313
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1836 2898 2949 2973 5332 1836
Size: 1062 51 24 2359 3817
Fragments arranged by size:
3817 2359 1062 51 24
FspI TGC'GCA
Cuts at: 1312 1492 1783 5094 1312
Size: 180 291 3311 3531
Fragments arranged by size:
3531 3311 291 180
HaeII r_GCGC'y
Cuts at: 502 576 884 1008 2459 2752 3674 3960 4162
Size: 74 308 124 1451 293 922 286 202
Cuts at: 4162 4747 4995 6017 6996 502
Size: 585 248 1022 979 819
Fragments arranged by size:
1451 1022 979 922 819 585 308 293
286 248 202 124 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 541 573 2149 2181 5155 5185 5187 5217 541
Size: 32 1576 32 2974 30 2 30 2637
Fragments arranged by size:
2974 2637 1576 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3186 3500 4352 3186
Size: 314 852 6147
Fragments arranged by size:
6147 852 314
KasI G'GCGC_C
Cuts at: 880 4158 4743 4991 880
Size: 3278 585 248 3202
Fragments arranged by size:
3278 3202 585 248
KpnI G_GTAC'C
Cuts at: 822 4333 822
Size: 3511 3802
MluI A'CGCG_T
Cuts at: 6746 6746
Size: 7313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2067 2252 5984 6168 2067
Size: 185 3732 184 3212
Fragments arranged by size:
3732 3212 185 184
MscI TGG'CCA
Cuts at: 1237 1417 1426 2899 4000 5074 1237
Size: 180 9 1473 1101 1074 3476
Fragments arranged by size:
3476 1473 1101 1074 180 9
MslI CAynn'nnrTG
Cuts at: 379 1411 1714 2425 3706 4105 5302 5429 379
Size: 1032 303 711 1281 399 1197 127 2263
Fragments arranged by size:
2263 1281 1197 1032 711 399 303 127
MspA1I CmG'CkG
Cuts at: 420 591 600 655 697 798 1104 1367 1538
Size: 171 9 55 42 101 306 263 171
Cuts at: 1538 1619 1779 2015 2243 2262 2312 2653 3435
Size: 81 160 236 228 19 50 341 782
Cuts at: 3435 3576 3715 4424 4738 5098 6111 6356 420
Size: 141 139 709 314 360 1013 245 1377
Fragments arranged by size:
1377 1013 782 709 360 341 314 306
263 245 236 228 171 171 160 141
139 101 81 55 50 42 19 9
NaeI GCC'GGC
Cuts at: 2371 2581 3122 5494 2371
Size: 210 541 2372 4190
Fragments arranged by size:
4190 2372 541 210
NarI GG'CG_CC
Cuts at: 881 4159 4744 4992 881
Size: 3278 585 248 3202
Fragments arranged by size:
3278 3202 585 248
NcoI C'CATG_G
Cuts at: 125 380 3152 3485 125
Size: 255 2772 333 3953
Fragments arranged by size:
3953 2772 333 255
NgoMIV G'CCGG_C
Cuts at: 2369 2579 3120 5492 2369
Size: 210 541 2372 4190
Fragments arranged by size:
4190 2372 541 210
NheI G'CTAG_C
Cuts at: 2575 2575
Size: 7313
NsiI A_TGCA'T
Cuts at: 2977 2977
Size: 7313
NspI r_CATG'y
Cuts at: 1515 4366 4866 5773 1515
Size: 2851 500 907 3055
Fragments arranged by size:
3055 2851 907 500
PasI CC'CwG_GG
Cuts at: 315 2103 2570 4203 4230 315
Size: 1788 467 1633 27 3398
Fragments arranged by size:
3398 1788 1633 467 27
PciI A'CATG_T
Cuts at: 5769 5769
Size: 7313
PflMI CCAn_nnn'nTGG
Cuts at: 1206 1668 1206
Size: 462 6851
PfoI T'CCnGG_A
Cuts at: 1058 2495 1058
Size: 1437 5876
PmeI GTTT'AAAC
Cuts at: 4312 4312
Size: 7313
PmlI CAC'GTG
Cuts at: 1647 3990 1647
Size: 2343 4970
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6452 6484 6452
Size: 32 7281
PpuMI rG'GwC_Cy
Cuts at: 1203 2286 2565 1203
Size: 1083 279 5951
Fragments arranged by size:
5951 1083 279
PspOMI G'GGCC_C
Cuts at: 215 229 2106 2390 3335 4226 215
Size: 14 1877 284 945 891 3302
Fragments arranged by size:
3302 1877 945 891 284 14
PstI C_TGCA'G
Cuts at: 155 373 596 1287 1809 1959 2219 2267 2519
Size: 218 223 691 522 150 260 48 252
Cuts at: 2519 2600 3380 3581 4262 4360 5045 155
Size: 81 780 201 681 98 685 2423
Fragments arranged by size:
2423 780 691 685 681 522 260 252
223 218 201 150 98 81 48
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7313
PvuII CAG'CTG
Cuts at: 591 600 1538 1619 1779 2015 2262 3435 3576
Size: 9 938 81 160 236 247 1173 141
Cuts at: 3576 4738 5098 591
Size: 1162 360 2806
Fragments arranged by size:
2806 1173 1162 938 360 247 236 160
141 81 9
RsrII CG'GwC_CG
Cuts at: 5508 5508
Size: 7313
SacI G_AGCT'C
Cuts at: 956 1303 2002 4327 956
Size: 347 699 2325 3942
Fragments arranged by size:
3942 2325 699 347
SacII CC_GC'GG
Cuts at: 656 698 2244 3716 656
Size: 42 1546 1472 4253
Fragments arranged by size:
4253 1546 1472 42
SalI G'TCGA_C
Cuts at: 3498 4350 3498
Size: 852 6461
SanDI GG'GwC_CC
Cuts at: 2565 2565
Size: 7313
SapI GCTCTTCn'nnn_
Cuts at: 364 1291 2150 5336 5546 364
Size: 927 859 3186 210 2131
Fragments arranged by size:
3186 2131 927 859 210
SbfI CC_TGCA'GG
Cuts at: 2600 4360 2600
Size: 1760 5553
ScaI AGT'ACT
Cuts at: 1666 6830 1666
Size: 5164 2149
SexAI A'CCwGG_T
Cuts at: 3887 3887
Size: 7313
SfcI C'TryA_G
Cuts at: 33 151 369 592 1283 1805 1955 2215 2263
Size: 118 218 223 691 522 150 260 48
Cuts at: 2263 2515 2596 2658 2792 3357 3376 3577 4258
Size: 252 81 62 134 565 19 201 681
Cuts at: 4258 4356 5041 6034 6225 33
Size: 98 685 993 191 1121
Fragments arranged by size:
1121 993 691 685 681 565 522 260
252 223 218 201 191 150 134 118
98 81 62 48 19
SfiI GGCCn_nnn'nGGCC
Cuts at: 1423 3718 1423
Size: 2295 5018
SfoI GGC'GCC
Cuts at: 882 4160 4745 4993 882
Size: 3278 585 248 3202
Fragments arranged by size:
3278 3202 585 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7313
SmaI CCC'GGG
Cuts at: 3227 3428 4335 3227
Size: 201 907 6205
Fragments arranged by size:
6205 907 201
SmlI C'TyrA_G
Cuts at: 502 1090 2440 4195 4468 5383 5875 6137 502
Size: 588 1350 1755 273 915 492 262 1678
Fragments arranged by size:
1755 1678 1350 915 588 492 273 262
SnaBI TAC'GTA
Cuts at: 4305 4305
Size: 7313
SpeI A'CTAG_T
Cuts at: 67 3135 67
Size: 3068 4245
SphI G_CATG'C
Cuts at: 4366 4866 4366
Size: 500 6813
StuI AGG'CCT
Cuts at: 469 3583 469
Size: 3114 4199
StyI C'CwwG_G
Cuts at: 125 380 2692 2846 2903 3059 3152 3485 4447
Size: 255 2312 154 57 156 93 333 962
Cuts at: 4447 4801 125
Size: 354 2637
Fragments arranged by size:
2637 2312 962 354 333 255 156 154
93 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1064 1774 2304 3748 4195 4228 4896 5029 5488
Size: 710 530 1444 447 33 668 133 459
Cuts at: 5488 5697 1064
Size: 209 2680
Fragments arranged by size:
2680 1444 710 668 530 459 447 209
133 33
TatI w'GTAC_w
Cuts at: 1664 2444 2618 2930 3803 6828 1664
Size: 780 174 312 873 3025 2149
Fragments arranged by size:
3025 2149 873 780 312 174
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6830 6862 6830
Size: 32 7281
Tth111I GACn'n_nGTC
Cuts at: 1723 5110 1723
Size: 3387 3926
XbaI T'CTAG_A
Cuts at: 4344 4344
Size: 7313
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1005 1456 1651 2118 3159 3315 4808 1005
Size: 451 195 467 1041 156 1493 3510
Fragments arranged by size:
3510 1493 1041 467 451 195 156
XmaI C'CCGG_G
Cuts at: 3225 3426 4333 3225
Size: 201 907 6205
Fragments arranged by size:
6205 907 201
ZraI GAC'GTC
Cuts at: 2534 2534
Size: 7313
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AhdI AloI AscI AvrII BaeI BsmI BstZ17I
ClaI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NotI NruI PacI PshAI PsiI PspXI PsrI SgrAI
SrfI SspI SwaI XhoI XmnI