Restriction map for
pF1KSDA1341
(Circular) (Six-base) MAPSORT of: pF1KSDA1341.seq Check: 5152 from: 1 to: 4937
pF1KSDA1341 4937 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 191 191
Size: 4937
AccI GT'mk_AC
Cuts at: 813 1425 1975 813
Size: 612 550 3775
Fragments arranged by size:
3775 612 550
Acc65I G'GTAC_C
Cuts at: 1953 1953
Size: 4937
AclI AA'CG_TT
Cuts at: 4777 4777
Size: 4937
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 806 2762 3194 3941 4209 806
Size: 1956 432 747 268 1534
Fragments arranged by size:
1956 1534 747 432 268
AflIII A'CryG_T
Cuts at: 714 2289 3393 4370 714
Size: 1575 1104 977 1281
Fragments arranged by size:
1575 1281 1104 977
AgeI A'CCGG_T
Cuts at: 3325 3325
Size: 4937
AlwNI CAG_nnn'CTG
Cuts at: 2242 3809 2242
Size: 1567 3370
ApaLI G'TGCA_C
Cuts at: 3707 3707
Size: 4937
ApoI r'AATT_y
Cuts at: 387 565 580 744 1197 1750 1941 4290 4546
Size: 178 15 164 453 553 191 2349 256
Cuts at: 4546 387
Size: 778
Fragments arranged by size:
2349 778 553 453 256 191 178 164
15
AseI AT'TA_AT
Cuts at: 20 20
Size: 4937
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4937
AvaI C'yCGr_G
Cuts at: 154 1957 154
Size: 1803 3134
BamHI G'GATC_C
Cuts at: 651 1962 651
Size: 1311 3626
BanI G'GyrC_C
Cuts at: 150 1953 2367 2615 2650 150
Size: 1803 414 248 35 2437
Fragments arranged by size:
2437 1803 414 248 35
BanII G_rGCy'C
Cuts at: 521 1951 2981 521
Size: 1430 1030 2477
Fragments arranged by size:
2477 1430 1030
BbeI G_GCGC'C
Cuts at: 2371 2619 2371
Size: 248 4689
BbsI GAAGACnn'nnnn_
Cuts at: 636 929 636
Size: 293 4644
BciVI GTATCCnnnnn_n'
Cuts at: 2835 3596 4937 2835
Size: 761 1341 2835
Fragments arranged by size:
2835 1341 761
BclI T'GATC_A
Cuts at: 2457 2457
Size: 4937
BfrBI ATG'CAT
Cuts at: 1035 1924 1035
Size: 889 4048
BglI GCCn_nnn'nGGC
Cuts at: 142 142
Size: 4937
BglII A'GATC_T
Cuts at: 477 2452 4489 477
Size: 1975 2037 925
Fragments arranged by size:
2037 1975 925
BlpI GC'TnA_GC
Cuts at: 2049 2049
Size: 4937
Bme1580I G_kGCm'C
Cuts at: 155 397 955 2562 2655 3711 155
Size: 242 558 1607 93 1056 1381
Fragments arranged by size:
1607 1381 1056 558 242 93
BmgBI CAC'GTC
Cuts at: 717 1381 717
Size: 664 4273
BmrI ACTGGGnnnn_n'
Cuts at: 2149 2230 2565 4709 2149
Size: 81 335 2144 2377
Fragments arranged by size:
2377 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3304 3304
Size: 4937
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 419 1556 419
Size: 1137 3800
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2113 2992 3484 3782 2113
Size: 879 492 298 3268
Fragments arranged by size:
3268 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4641 4641
Size: 4937
BsaAI yAC'GTr
Cuts at: 513 1111 1929 2290 513
Size: 598 818 361 3160
Fragments arranged by size:
3160 818 598 361
BsaBI GATnn'nnATC
Cuts at: 6 1628 1641 2451 4488 6
Size: 1622 13 810 2037 455
Fragments arranged by size:
2037 1622 810 455 13
BsaHI Gr'CG_yC
Cuts at: 134 2368 2616 3318 4814 134
Size: 2234 248 702 1496 257
Fragments arranged by size:
2234 1496 702 257 248
BsaWI w'CCGG_w
Cuts at: 1559 2126 2647 3325 3599 3746 4157 1559
Size: 567 521 678 274 147 411 2339
Fragments arranged by size:
2339 678 567 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 701 731 701
Size: 30 4907
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1274 1328 1274
Size: 54 4883
BseYI C'CCAG_C
Cuts at: 2362 3120 3697 4193 2362
Size: 758 577 496 3106
Fragments arranged by size:
3106 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2479 2525 3733 104
Size: 2375 46 1208 1308
Fragments arranged by size:
2375 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 521 1951 2729 2919 3711 521
Size: 1430 778 190 792 1747
Fragments arranged by size:
1747 1430 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 155 397 521 955 1951 2562 2655 2729 2919
Size: 242 124 434 996 611 93 74 190
Cuts at: 2919 2981 3711 155
Size: 62 730 1381
Fragments arranged by size:
1381 996 730 611 434 242 190 124
93 74 62
BspEI T'CCGG_A
Cuts at: 4157 4157
Size: 4937
BspHI T'CATG_A
Cuts at: 1065 4113 1065
Size: 3048 1889
BspMI ACCTGCnnnn'nnnn_
Cuts at: 191 1987 2201 2503 2884 191
Size: 1796 214 302 381 2244
Fragments arranged by size:
2244 1796 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3229 3283 4767 4932 50
Size: 3179 54 1484 165 55
Fragments arranged by size:
3179 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 679 1295 1445 2849 679
Size: 616 150 1404 2767
Fragments arranged by size:
2767 1404 616 150
BsrFI r'CCGG_y
Cuts at: 808 2935 3116 3325 808
Size: 2127 181 209 2420
Fragments arranged by size:
2420 2127 209 181
BssSI C'ACGA_G
Cuts at: 3208 3566 3208
Size: 358 4579
BstAPI GCAn_nnn'nTGC
Cuts at: 2314 2314
Size: 4937
BstBI TT'CG_AA
Cuts at: 110 3298 110
Size: 3188 1749
BstYI r'GATC_y
Cuts at: 1 477 651 1468 1492 1962 2429 2452 2787
Size: 476 174 817 24 470 467 23 335
Cuts at: 2787 3033 4045 4131 4143 4489 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 817 476 470 467 449 346 335
246 174 86 24 23 12
BtgI C'CryG_G
Cuts at: 125 211 230 1341 125
Size: 86 19 1111 3721
Fragments arranged by size:
3721 1111 86 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1363 2887 3068 3231 1363
Size: 1524 181 163 3069
Fragments arranged by size:
3069 1524 181 163
BtsI GCAGTG_nn'
Cuts at: 1440 2180 1440
Size: 740 4197
ClaI AT'CG_AT
Cuts at: 1622 1622
Size: 4937
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 813 848 813
Size: 35 4902
DraI TTT'AAA
Cuts at: 1936 1936
Size: 4937
DrdI GACnn_nn'nnGTC
Cuts at: 2643 3501 2643
Size: 858 4079
EaeI y'GGCC_r
Cuts at: 1320 2522 2696 3087 3114 4459 1320
Size: 1202 174 391 27 1345 1798
Fragments arranged by size:
1798 1345 1202 391 174 27
EagI C'GGCC_G
Cuts at: 2522 2522
Size: 4937
EarI CTCTTCn'nnn_
Cuts at: 305 1064 2960 3170 305
Size: 759 1896 210 2072
Fragments arranged by size:
2072 1896 759 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 145 253 3145 3455 3601 4747 145
Size: 108 2892 310 146 1146 335
Fragments arranged by size:
2892 1146 335 310 146 108
EcoICRI GAG'CTC
Cuts at: 519 1949 519
Size: 1430 3507
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 419 806 1556 2762 3194 3941 4209 419
Size: 387 750 1206 432 747 268 1147
Fragments arranged by size:
1206 1147 750 747 432 387 268
EcoO109I rG'GnC_Cy
Cuts at: 2076 2076
Size: 4937
EcoRI G'AATT_C
Cuts at: 387 565 1941 387
Size: 178 1376 3383
Fragments arranged by size:
3383 1376 178
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2956 2956
Size: 4937
FspI TGC'GCA
Cuts at: 2718 2718
Size: 4937
HaeII r_GCGC'y
Cuts at: 2371 2619 3641 4620 2371
Size: 248 1022 979 2688
Fragments arranged by size:
2688 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 701 715 733 747 1039 1071 2779 2809 2811
Size: 14 18 14 292 32 1708 30 2
Cuts at: 2811 2841 701
Size: 30 2797
Fragments arranged by size:
2797 1708 292 32 30 30 18 14
14 2
HincII GTy'rAC
Cuts at: 1976 1976
Size: 4937
KasI G'GCGC_C
Cuts at: 2367 2615 2367
Size: 248 4689
KpnI G_GTAC'C
Cuts at: 1957 1957
Size: 4937
MfeI C'AATT_G
Cuts at: 974 974
Size: 4937
MluI A'CGCG_T
Cuts at: 4370 4370
Size: 4937
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 841 3608 3792 841
Size: 2767 184 1986
Fragments arranged by size:
2767 1986 184
MscI TGG'CCA
Cuts at: 2698 2698
Size: 4937
MslI CAynn'nnrTG
Cuts at: 1129 1624 2926 3053 1129
Size: 495 1302 127 3013
Fragments arranged by size:
3013 1302 495 127
MspA1I CmG'CkG
Cuts at: 213 232 1849 2048 2362 2722 3735 3980 213
Size: 19 1617 199 314 360 1013 245 1170
Fragments arranged by size:
1617 1170 1013 360 314 245 199 19
NaeI GCC'GGC
Cuts at: 3118 3118
Size: 4937
NarI GG'CG_CC
Cuts at: 2368 2616 2368
Size: 248 4689
NcoI C'CATG_G
Cuts at: 125 125
Size: 4937
NdeI CA'TA_TG
Cuts at: 450 450
Size: 4937
NgoMIV G'CCGG_C
Cuts at: 3116 3116
Size: 4937
NsiI A_TGCA'T
Cuts at: 1037 1926 1037
Size: 889 4048
NspI r_CATG'y
Cuts at: 1039 1990 2490 3397 1039
Size: 951 500 907 2579
Fragments arranged by size:
2579 951 907 500
PciI A'CATG_T
Cuts at: 3393 3393
Size: 4937
PflMI CCAn_nnn'nTGG
Cuts at: 1237 1237
Size: 4937
PfoI T'CCnGG_A
Cuts at: 705 705
Size: 4937
PmeI GTTT'AAAC
Cuts at: 1936 1936
Size: 4937
PmlI CAC'GTG
Cuts at: 1111 1111
Size: 4937
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4076 4108 4076
Size: 32 4905
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 804 836 804
Size: 32 4905
PstI C_TGCA'G
Cuts at: 188 1864 1984 2669 188
Size: 1676 120 685 2456
Fragments arranged by size:
2456 1676 685 120
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4937
PvuII CAG'CTG
Cuts at: 1849 2362 2722 1849
Size: 513 360 4064
Fragments arranged by size:
4064 513 360
RsrII CG'GwC_CG
Cuts at: 3132 3132
Size: 4937
SacI G_AGCT'C
Cuts at: 521 1951 521
Size: 1430 3507
SacII CC_GC'GG
Cuts at: 214 233 214
Size: 19 4918
SalI G'TCGA_C
Cuts at: 1974 1974
Size: 4937
SapI GCTCTTCn'nnn_
Cuts at: 2960 3170 2960
Size: 210 4727
SbfI CC_TGCA'GG
Cuts at: 1984 1984
Size: 4937
ScaI AGT'ACT
Cuts at: 4454 4454
Size: 4937
SexAI A'CCwGG_T
Cuts at: 1590 1590
Size: 4937
SfcI C'TryA_G
Cuts at: 33 184 338 1860 1980 2665 3658 3849 33
Size: 151 154 1522 120 685 993 191 1121
Fragments arranged by size:
1522 1121 993 685 191 154 151 120
SfoI GGC'GCC
Cuts at: 2369 2617 2369
Size: 248 4689
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4937
SmaI CCC'GGG
Cuts at: 156 1959 156
Size: 1803 3134
SmlI C'TyrA_G
Cuts at: 2092 3007 3499 3761 2092
Size: 915 492 262 3268
Fragments arranged by size:
3268 915 492 262
SnaBI TAC'GTA
Cuts at: 1929 1929
Size: 4937
SpeI A'CTAG_T
Cuts at: 67 1354 67
Size: 1287 3650
SphI G_CATG'C
Cuts at: 1990 2490 1990
Size: 500 4437
SspI AAT'ATT
Cuts at: 642 642
Size: 4937
StyI C'CwwG_G
Cuts at: 125 2071 2425 125
Size: 1946 354 2637
Fragments arranged by size:
2637 1946 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2520 2653 3112 3321 2520
Size: 133 459 209 4136
Fragments arranged by size:
4136 459 209 133
TatI w'GTAC_w
Cuts at: 4452 4452
Size: 4937
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4454 4486 4454
Size: 32 4905
Tth111I GACn'n_nGTC
Cuts at: 1139 2734 1139
Size: 1595 3342
XbaI T'CTAG_A
Cuts at: 1968 1968
Size: 4937
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2432 2432
Size: 4937
XmaI C'CCGG_G
Cuts at: 154 1957 154
Size: 1803 3134
XmnI GAAnn'nnTTC
Cuts at: 983 983
Size: 4937
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PciI PflMI
PfoI PmeI PmlI PpiI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BmtI Bpu10I BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
NheI NotI NruI PacI PasI PpuMI PshAI PsiI
PspOMI PspXI SanDI SfiI SgrAI SrfI StuI SwaI
XhoI ZraI