Restriction map for
pF1KSDA1345
(Circular) (Six-base) MAPSORT of: pF1KSDA1345.seq Check: 6638 from: 1 to: 7673
pF1KSDA1345 7673 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 678 678
Size: 7673
AccI GT'mk_AC
Cuts at: 3749 4592 4711 3749
Size: 843 119 6711
Fragments arranged by size:
6711 843 119
Acc65I G'GTAC_C
Cuts at: 4689 4689
Size: 7673
AclI AA'CG_TT
Cuts at: 7513 7513
Size: 7673
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2140 2276 3350 4262 4529 5498 5930 6677 6945
Size: 136 1074 912 267 969 432 747 268
Cuts at: 6945 2140
Size: 2868
Fragments arranged by size:
2868 1074 969 912 747 432 268 267
136
AfeI AGC'GCT
Cuts at: 2017 4261 2017
Size: 2244 5429
AflII C'TTAA_G
Cuts at: 1495 1495
Size: 7673
AflIII A'CryG_T
Cuts at: 3703 5025 6129 7106 3703
Size: 1322 1104 977 4270
Fragments arranged by size:
4270 1322 1104 977
AgeI A'CCGG_T
Cuts at: 6061 6061
Size: 7673
AhdI GACnn_n'nnGTC
Cuts at: 2069 2069
Size: 7673
AlwNI CAG_nnn'CTG
Cuts at: 4978 6545 4978
Size: 1567 6106
ApaLI G'TGCA_C
Cuts at: 6443 6443
Size: 7673
ApoI r'AATT_y
Cuts at: 268 403 1185 1321 1439 1456 1631 2746 3163
Size: 135 782 136 118 17 175 1115 417
Cuts at: 3163 4158 4576 4677 7026 7282 268
Size: 995 418 101 2349 256 659
Fragments arranged by size:
2349 1115 995 782 659 418 417 256
175 136 135 118 101 17
AseI AT'TA_AT
Cuts at: 20 20
Size: 7673
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7673
AvaI C'yCGr_G
Cuts at: 1813 1992 4693 1813
Size: 179 2701 4793
Fragments arranged by size:
4793 2701 179
BamHI G'GATC_C
Cuts at: 1025 4698 1025
Size: 3673 4000
BanI G'GyrC_C
Cuts at: 2739 4689 5103 5351 5386 2739
Size: 1950 414 248 35 5026
Fragments arranged by size:
5026 1950 414 248 35
BanII G_rGCy'C
Cuts at: 182 1850 1922 2250 3381 3740 4687 5717 182
Size: 1668 72 328 1131 359 947 1030 2138
Fragments arranged by size:
2138 1668 1131 1030 947 359 328 72
BbeI G_GCGC'C
Cuts at: 5107 5355 5107
Size: 248 7425
BbsI GAAGACnn'nnnn_
Cuts at: 3176 3176
Size: 7673
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3293 3327 3293
Size: 34 7639
BciVI GTATCCnnnnn_n'
Cuts at: 1820 2411 5571 6332 7673 1820
Size: 591 3160 761 1341 1820
Fragments arranged by size:
3160 1820 1341 761 591
BclI T'GATC_A
Cuts at: 1160 3864 5193 1160
Size: 2704 1329 3640
Fragments arranged by size:
3640 2704 1329
BglI GCCn_nnn'nGGC
Cuts at: 1270 2539 1270
Size: 1269 6404
BglII A'GATC_T
Cuts at: 1165 5188 7225 1165
Size: 4023 2037 1613
Fragments arranged by size:
4023 2037 1613
BlpI GC'TnA_GC
Cuts at: 1939 4283 4785 1939
Size: 2344 502 4827
Fragments arranged by size:
4827 2344 502
Bme1580I G_kGCm'C
Cuts at: 1978 2324 5298 5391 6447 1978
Size: 346 2974 93 1056 3204
Fragments arranged by size:
3204 2974 1056 346 93
BmrI ACTGGGnnnn_n'
Cuts at: 374 839 1269 4885 4966 5301 7445 374
Size: 465 430 3616 81 335 2144 602
Fragments arranged by size:
3616 2144 602 465 430 335 81
BmtI G_CTAG'C
Cuts at: 2200 2200
Size: 7673
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 248 850 6040 248
Size: 602 5190 1881
Fragments arranged by size:
5190 1881 602
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1190 1749 1826 2668 2878 3873 4353 4565 1190
Size: 559 77 842 210 995 480 212 4298
Fragments arranged by size:
4298 995 842 559 480 212 210 77
Bpu10I CC'TnA_GC
Cuts at: 3004 3004
Size: 7673
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 453 4849 5728 6220 6518 453
Size: 4396 879 492 298 1608
Fragments arranged by size:
4396 1608 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1166 1995 7377 1166
Size: 829 5382 1462
Fragments arranged by size:
5382 1462 829
BsaAI yAC'GTr
Cuts at: 756 3227 4665 5026 756
Size: 2471 1438 361 3403
Fragments arranged by size:
3403 2471 1438 361
BsaBI GATnn'nnATC
Cuts at: 6 4140 5187 7224 6
Size: 4134 1047 2037 455
Fragments arranged by size:
4134 2037 1047 455
BsaHI Gr'CG_yC
Cuts at: 5104 5352 6054 7550 5104
Size: 248 702 1496 5227
Fragments arranged by size:
5227 1496 702 248
BsaWI w'CCGG_w
Cuts at: 209 4862 5383 6061 6335 6482 6893 209
Size: 4653 521 678 274 147 411 989
Fragments arranged by size:
4653 989 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1545 1575 3042 3072 4409 4439 1545
Size: 30 1467 30 1337 30 4779
Fragments arranged by size:
4779 1467 1337 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 190 344 655 658 695 1687 1831 1920 3719
Size: 154 311 3 37 992 144 89 1799
Cuts at: 3719 190
Size: 4144
Fragments arranged by size:
4144 1799 992 311 154 144 89 37
3
BseYI C'CCAG_C
Cuts at: 1834 3525 4461 5098 5856 6433 6929 1834
Size: 1691 936 637 758 577 496 2578
Fragments arranged by size:
2578 1691 936 758 637 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 476 656 190
Size: 286 180 7207
Fragments arranged by size:
7207 286 180
BsiEI CG_ry'CG
Cuts at: 104 5215 5261 6469 104
Size: 5111 46 1208 1308
Fragments arranged by size:
5111 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1667 3740 3859 4687 5465 5655 6447 1667
Size: 2073 119 828 778 190 792 2893
Fragments arranged by size:
2893 2073 828 792 778 190 119
Bsp1286I G_dGCh'C
Cuts at: 182 1667 1850 1922 1978 2250 2324 3381 3740
Size: 1485 183 72 56 272 74 1057 359
Cuts at: 3740 3859 4687 5298 5391 5465 5655 5717 6447
Size: 119 828 611 93 74 190 62 730
Cuts at: 6447 182
Size: 1408
Fragments arranged by size:
1485 1408 1057 828 730 611 359 272
190 183 119 93 74 74 72 62
56
BspEI T'CCGG_A
Cuts at: 209 6893 209
Size: 6684 989
BspHI T'CATG_A
Cuts at: 6849 6849
Size: 7673
BspMI ACCTGCnnnn'nnnn_
Cuts at: 678 792 2889 4723 4937 5239 5620 678
Size: 114 2097 1834 214 302 381 2731
Fragments arranged by size:
2731 2097 1834 381 302 214 114
BsrBI CCG'CTC
Cuts at: 50 625 1984 5965 6019 7503 7668 50
Size: 575 1359 3981 54 1484 165 55
Fragments arranged by size:
3981 1484 1359 575 165 55 54
BsrDI GCAATG_nn'
Cuts at: 331 1489 3972 5585 331
Size: 1158 2483 1613 2419
Fragments arranged by size:
2483 2419 1613 1158
BsrFI r'CCGG_y
Cuts at: 3150 5671 5852 6061 3150
Size: 2521 181 209 4762
Fragments arranged by size:
4762 2521 209 181
BsrGI T'GTAC_A
Cuts at: 888 888
Size: 7673
BssSI C'ACGA_G
Cuts at: 2299 5944 6302 2299
Size: 3645 358 3670
Fragments arranged by size:
3670 3645 358
BstAPI GCAn_nnn'nTGC
Cuts at: 328 5050 328
Size: 4722 2951
BstBI TT'CG_AA
Cuts at: 110 6034 110
Size: 5924 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 903 903
Size: 7673
BstYI r'GATC_y
Cuts at: 1 1025 1165 2698 4631 4698 5165 5188 5523
Size: 1024 140 1533 1933 67 467 23 335
Cuts at: 5523 5769 6781 6867 6879 7225 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1933 1533 1024 1012 467 449 346 335
246 140 86 67 23 12
BstZ17I GTA'TAC
Cuts at: 4593 4593
Size: 7673
Bsu36I CC'TnA_GG
Cuts at: 2370 3731 3977 2370
Size: 1361 246 6066
Fragments arranged by size:
6066 1361 246
BtgI C'CryG_G
Cuts at: 125 220 125
Size: 95 7578
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5623 5804 5967 5623
Size: 181 163 7329
Fragments arranged by size:
7329 181 163
BtsI GCAGTG_nn'
Cuts at: 1868 3037 3161 4047 4916 1868
Size: 1169 124 886 869 4625
Fragments arranged by size:
4625 1169 886 869 124
DraI TTT'AAA
Cuts at: 3722 4672 3722
Size: 950 6723
DrdI GACnn_nn'nnGTC
Cuts at: 2183 5379 6237 2183
Size: 3196 858 3619
Fragments arranged by size:
3619 3196 858
EaeI y'GGCC_r
Cuts at: 2074 2137 2531 3043 3261 5258 5432 5823 5850
Size: 63 394 512 218 1997 174 391 27
Cuts at: 5850 7195 2074
Size: 1345 2552
Fragments arranged by size:
2552 1997 1345 512 394 391 218 174
63 27
EagI C'GGCC_G
Cuts at: 5258 5258
Size: 7673
EarI CTCTTCn'nnn_
Cuts at: 539 841 1655 4238 4496 5696 5906 539
Size: 302 814 2583 258 1200 210 2306
Fragments arranged by size:
2583 2306 1200 814 302 258 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5881 6191 6337 7483 5881
Size: 310 146 1146 6071
Fragments arranged by size:
6071 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3738 4685 3738
Size: 947 6726
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1190 1749 1826 2140 2276 2668 2878 3350 3873
Size: 559 77 314 136 392 210 472 523
Cuts at: 3873 4262 4353 4529 4565 5498 5930 6677 6945
Size: 389 91 176 36 933 432 747 268
Cuts at: 6945 1190
Size: 1918
Fragments arranged by size:
1918 933 747 559 523 472 432 392
389 314 268 210 176 136 91 77
36
EcoNI CCTnn'n_nnAGG
Cuts at: 674 2896 3262 674
Size: 2222 366 5085
Fragments arranged by size:
5085 2222 366
EcoO109I rG'GnC_Cy
Cuts at: 990 1821 4105 4812 990
Size: 831 2284 707 3851
Fragments arranged by size:
3851 2284 831 707
EcoRI G'AATT_C
Cuts at: 2746 4677 2746
Size: 1931 5742
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 727 5692 727
Size: 4965 2708
FspI TGC'GCA
Cuts at: 5454 5454
Size: 7673
HaeII r_GCGC'y
Cuts at: 2019 4263 5107 5355 6377 7356 2019
Size: 2244 844 248 1022 979 2336
Fragments arranged by size:
2336 2244 1022 979 844 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1017 1049 1417 1449 1513 1545 1973 2005 2055
Size: 32 368 32 64 32 428 32 50
Cuts at: 2055 2087 2473 2505 3042 3074 3811 3843 5515
Size: 32 386 32 537 32 737 32 1672
Cuts at: 5515 5545 5547 5577 1017
Size: 30 2 30 3113
Fragments arranged by size:
3113 1672 737 537 428 386 368 64
50 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 453 2133 4712 453
Size: 1680 2579 3414
Fragments arranged by size:
3414 2579 1680
HindIII A'AGCT_T
Cuts at: 874 1343 874
Size: 469 7204
KasI G'GCGC_C
Cuts at: 5103 5351 5103
Size: 248 7425
KpnI G_GTAC'C
Cuts at: 4693 4693
Size: 7673
MluI A'CGCG_T
Cuts at: 7106 7106
Size: 7673
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1215 2154 2289 3040 4009 6344 6528 1215
Size: 939 135 751 969 2335 184 2360
Fragments arranged by size:
2360 2335 969 939 751 184 135
MscI TGG'CCA
Cuts at: 2533 5434 2533
Size: 2901 4772
MslI CAynn'nnrTG
Cuts at: 1787 3139 4052 5662 5789 1787
Size: 1352 913 1610 127 3671
Fragments arranged by size:
3671 1610 1352 913 127
MspA1I CmG'CkG
Cuts at: 2355 2655 4276 4282 4461 4784 5098 5458 6471
Size: 300 1621 6 179 323 314 360 1013
Cuts at: 6471 6716 2355
Size: 245 3312
Fragments arranged by size:
3312 1621 1013 360 323 314 300 245
179 6
NaeI GCC'GGC
Cuts at: 3152 5854 3152
Size: 2702 4971
NarI GG'CG_CC
Cuts at: 5104 5352 5104
Size: 248 7425
NcoI C'CATG_G
Cuts at: 125 125
Size: 7673
NdeI CA'TA_TG
Cuts at: 1620 1620
Size: 7673
NgoMIV G'CCGG_C
Cuts at: 3150 5852 3150
Size: 2702 4971
NheI G'CTAG_C
Cuts at: 2196 2196
Size: 7673
NspI r_CATG'y
Cuts at: 1159 1255 2287 3211 3907 4500 4726 5226 6133
Size: 96 1032 924 696 593 226 500 907
Cuts at: 6133 1159
Size: 2699
Fragments arranged by size:
2699 1032 924 907 696 593 500 226
96
PciI A'CATG_T
Cuts at: 6129 6129
Size: 7673
PflMI CCAn_nnn'nTGG
Cuts at: 4184 4219 4184
Size: 35 7638
PfoI T'CCnGG_A
Cuts at: 902 1179 1931 2059 2804 902
Size: 277 752 128 745 5771
Fragments arranged by size:
5771 752 745 277 128
PmeI GTTT'AAAC
Cuts at: 4672 4672
Size: 7673
PmlI CAC'GTG
Cuts at: 756 756
Size: 7673
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6812 6844 6812
Size: 32 7641
PpuMI rG'GwC_Cy
Cuts at: 990 1821 4105 990
Size: 831 2284 4558
Fragments arranged by size:
4558 2284 831
PsiI TTA'TAA
Cuts at: 2759 2759
Size: 7673
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1124 1156 3893 3925 1124
Size: 32 2737 32 4872
Fragments arranged by size:
4872 2737 32 32
PstI C_TGCA'G
Cuts at: 341 543 789 818 3413 3777 4042 4281 4720
Size: 202 246 29 2595 364 265 239 439
Cuts at: 4720 5405 341
Size: 685 2609
Fragments arranged by size:
2609 2595 685 439 364 265 246 239
202 29
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7673
PvuII CAG'CTG
Cuts at: 2355 2655 4276 4282 4461 5098 5458 2355
Size: 300 1621 6 179 637 360 4570
Fragments arranged by size:
4570 1621 637 360 300 179 6
RsrII CG'GwC_CG
Cuts at: 5868 5868
Size: 7673
SacI G_AGCT'C
Cuts at: 3740 4687 3740
Size: 947 6726
SalI G'TCGA_C
Cuts at: 4710 4710
Size: 7673
SapI GCTCTTCn'nnn_
Cuts at: 841 1655 4238 5696 5906 841
Size: 814 2583 1458 210 2608
Fragments arranged by size:
2608 2583 1458 814 210
SbfI CC_TGCA'GG
Cuts at: 4720 4720
Size: 7673
ScaI AGT'ACT
Cuts at: 1076 3455 3911 4542 7190 1076
Size: 2379 456 631 2648 1559
Fragments arranged by size:
2648 2379 1559 631 456
SfcI C'TryA_G
Cuts at: 33 337 539 785 814 3409 3763 3773 4038
Size: 304 202 246 29 2595 354 10 265
Cuts at: 4038 4277 4470 4716 5401 6394 6585 33
Size: 239 193 246 685 993 191 1121
Fragments arranged by size:
2595 1121 993 685 354 304 265 246
246 239 202 193 191 29 10
SfoI GGC'GCC
Cuts at: 5105 5353 5105
Size: 248 7425
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7673
SmaI CCC'GGG
Cuts at: 4695 4695
Size: 7673
SmlI C'TyrA_G
Cuts at: 468 1495 1992 4828 5743 6235 6497 468
Size: 1027 497 2836 915 492 262 1644
Fragments arranged by size:
2836 1644 1027 915 497 492 262
SnaBI TAC'GTA
Cuts at: 4665 4665
Size: 7673
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7673
SphI G_CATG'C
Cuts at: 3211 4726 5226 3211
Size: 1515 500 5658
Fragments arranged by size:
5658 1515 500
SspI AAT'ATT
Cuts at: 2944 4116 4128 4557 2944
Size: 1172 12 429 6060
Fragments arranged by size:
6060 1172 429 12
StuI AGG'CCT
Cuts at: 550 917 1726 1911 3949 550
Size: 367 809 185 2038 4274
Fragments arranged by size:
4274 2038 809 367 185
StyI C'CwwG_G
Cuts at: 125 147 1912 2849 3046 4807 5161 125
Size: 22 1765 937 197 1761 354 2637
Fragments arranged by size:
2637 1765 1761 937 354 197 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1977 2795 2881 4616 5256 5389 5848 6057 1977
Size: 818 86 1735 640 133 459 209 3593
Fragments arranged by size:
3593 1735 818 640 459 209 133 86
TatI w'GTAC_w
Cuts at: 888 1074 2024 3453 3909 4540 4548 7188 888
Size: 186 950 1429 456 631 8 2640 1373
Fragments arranged by size:
2640 1429 1373 950 631 456 186 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 515 547 1215 1247 2303 2335 3183 3215 7190
Size: 32 668 32 1056 32 848 32 3975
Cuts at: 7190 7222 515
Size: 32 966
Fragments arranged by size:
3975 1056 966 848 668 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 3823 5470 3823
Size: 1647 6026
XbaI T'CTAG_A
Cuts at: 4704 4704
Size: 7673
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 523 2305 3344 5168 523
Size: 1782 1039 1824 3028
Fragments arranged by size:
3028 1824 1782 1039
XhoI C'TCGA_G
Cuts at: 1992 1992
Size: 7673
XmaI C'CCGG_G
Cuts at: 4693 4693
Size: 7673
XmnI GAAnn'nnTTC
Cuts at: 2207 2978 4192 2207
Size: 771 1214 5688
Fragments arranged by size:
5688 1214 771
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PsiI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AleI AlfI AloI ApaI AscI AvrII BaeI
BbvCI BfrBI BmgBI BsiWI BsmI BsmBI BssHII BstEII
ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI
NotI NruI NsiI PacI PasI PshAI PspOMI PspXI
SacII SanDI SexAI SfiI SgrAI SrfI SwaI ZraI