Restriction map for
pF1KSDA1348
(Circular) (Six-base) MAPSORT of: pF1KSDA1348.seq Check: 7094 from: 1 to: 4724
pF1KSDA1348 4724 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 195 1762 195
Size: 1567 3157
Acc65I G'GTAC_C
Cuts at: 1740 1740
Size: 4724
AclI AA'CG_TT
Cuts at: 4564 4564
Size: 4724
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1204 2549 2981 3728 3996 1204
Size: 1345 432 747 268 1932
Fragments arranged by size:
1932 1345 747 432 268
AflIII A'CryG_T
Cuts at: 1042 2076 3180 4157 1042
Size: 1034 1104 977 1609
Fragments arranged by size:
1609 1104 1034 977
AgeI A'CCGG_T
Cuts at: 3112 3112
Size: 4724
AleI CACnn'nnGTG
Cuts at: 733 752 1666 733
Size: 19 914 3791
Fragments arranged by size:
3791 914 19
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 948 948
Size: 4724
AlwNI CAG_nnn'CTG
Cuts at: 633 771 837 1485 2029 3596 633
Size: 138 66 648 544 1567 1761
Fragments arranged by size:
1761 1567 648 544 138 66
ApaLI G'TGCA_C
Cuts at: 3494 3494
Size: 4724
ApoI r'AATT_y
Cuts at: 156 435 953 1728 4077 4333 156
Size: 279 518 775 2349 256 547
Fragments arranged by size:
2349 775 547 518 279 256
AseI AT'TA_AT
Cuts at: 20 817 20
Size: 797 3927
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4724
AvaI C'yCGr_G
Cuts at: 254 1744 254
Size: 1490 3234
BamHI G'GATC_C
Cuts at: 1749 1749
Size: 4724
BanI G'GyrC_C
Cuts at: 258 1412 1740 2154 2402 2437 258
Size: 1154 328 414 248 35 2545
Fragments arranged by size:
2545 1154 414 328 248 35
BanII G_rGCy'C
Cuts at: 1475 1514 1738 2768 1475
Size: 39 224 1030 3431
Fragments arranged by size:
3431 1030 224 39
BbeI G_GCGC'C
Cuts at: 2158 2406 2158
Size: 248 4476
BbvCI CC'TCA_GC
Cuts at: 1324 1421 1324
Size: 97 4627
BciVI GTATCCnnnnn_n'
Cuts at: 2622 3383 4724 2622
Size: 761 1341 2622
Fragments arranged by size:
2622 1341 761
BclI T'GATC_A
Cuts at: 1547 2244 1547
Size: 697 4027
BfrBI ATG'CAT
Cuts at: 565 565
Size: 4724
BglII A'GATC_T
Cuts at: 2239 4276 2239
Size: 2037 2687
BlpI GC'TnA_GC
Cuts at: 1298 1378 1836 1298
Size: 80 458 4186
Fragments arranged by size:
4186 458 80
Bme1580I G_kGCm'C
Cuts at: 573 1415 2349 2442 3498 573
Size: 842 934 93 1056 1799
Fragments arranged by size:
1799 1056 934 842 93
BmgBI CAC'GTC
Cuts at: 1392 1392
Size: 4724
BmrI ACTGGGnnnn_n'
Cuts at: 475 1150 1936 2017 2352 4496 475
Size: 675 786 81 335 2144 703
Fragments arranged by size:
2144 786 703 675 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3091 3091
Size: 4724
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 192 655 889 1237 192
Size: 463 234 348 3679
Fragments arranged by size:
3679 463 348 234
Bpu10I CC'TnA_GC
Cuts at: 362 1324 1421 362
Size: 962 97 3665
Fragments arranged by size:
3665 962 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 679 1900 2779 3271 3569 679
Size: 1221 879 492 298 1834
Fragments arranged by size:
1834 1221 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1449 4428 1449
Size: 2979 1745
BsaAI yAC'GTr
Cuts at: 966 1045 1716 2077 966
Size: 79 671 361 3613
Fragments arranged by size:
3613 671 361 79
BsaBI GATnn'nnATC
Cuts at: 6 2238 4275 6
Size: 2232 2037 455
Fragments arranged by size:
2232 2037 455
BsaHI Gr'CG_yC
Cuts at: 1145 2155 2403 3105 4601 1145
Size: 1010 248 702 1496 1268
Fragments arranged by size:
1496 1268 1010 702 248
BsaWI w'CCGG_w
Cuts at: 1913 2434 3112 3386 3533 3944 1913
Size: 521 678 274 147 411 2693
Fragments arranged by size:
2693 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 2149 2907 3484 3980 2149
Size: 758 577 496 2893
Fragments arranged by size:
2893 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2266 2312 3520 104
Size: 2162 46 1208 1308
Fragments arranged by size:
2162 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 641 1097 1738 2516 2706 3498 641
Size: 456 641 778 190 792 1867
Fragments arranged by size:
1867 792 778 641 456 190
BsmI GAATG_Cn'
Cuts at: 1211 1211
Size: 4724
Bsp1286I G_dGCh'C
Cuts at: 573 641 1097 1415 1475 1514 1738 2349 2442
Size: 68 456 318 60 39 224 611 93
Cuts at: 2442 2516 2706 2768 3498 573
Size: 74 190 62 730 1799
Fragments arranged by size:
1799 730 611 456 318 224 190 93
74 68 62 60 39
BspEI T'CCGG_A
Cuts at: 3944 3944
Size: 4724
BspHI T'CATG_A
Cuts at: 3900 3900
Size: 4724
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1774 1988 2290 2671 1774
Size: 214 302 381 3827
Fragments arranged by size:
3827 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1596 3016 3070 4554 4719 50
Size: 1546 1420 54 1484 165 55
Fragments arranged by size:
1546 1484 1420 165 55 54
BsrDI GCAATG_nn'
Cuts at: 173 181 1389 1615 2636 173
Size: 8 1208 226 1021 2261
Fragments arranged by size:
2261 1208 1021 226 8
BsrFI r'CCGG_y
Cuts at: 491 2722 2903 3112 491
Size: 2231 181 209 2103
Fragments arranged by size:
2231 2103 209 181
BsrGI T'GTAC_A
Cuts at: 1640 1640
Size: 4724
BssSI C'ACGA_G
Cuts at: 1516 2995 3353 1516
Size: 1479 358 2887
Fragments arranged by size:
2887 1479 358
BstAPI GCAn_nnn'nTGC
Cuts at: 771 1485 2101 771
Size: 714 616 3394
Fragments arranged by size:
3394 714 616
BstBI TT'CG_AA
Cuts at: 110 3085 110
Size: 2975 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 949 949
Size: 4724
BstYI r'GATC_y
Cuts at: 1 149 1749 2216 2239 2574 2820 3832 3918
Size: 148 1600 467 23 335 246 1012 86
Cuts at: 3918 3930 4276 1
Size: 12 346 449
Fragments arranged by size:
1600 1012 467 449 346 335 246 148
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1304 1304
Size: 4724
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1621 2674 2855 3018 1621
Size: 1053 181 163 3327
Fragments arranged by size:
3327 1053 181 163
BtsI GCAGTG_nn'
Cuts at: 584 614 1967 584
Size: 30 1353 3341
Fragments arranged by size:
3341 1353 30
DraI TTT'AAA
Cuts at: 1723 1723
Size: 4724
DrdI GACnn_nn'nnGTC
Cuts at: 557 2430 3288 557
Size: 1873 858 1993
Fragments arranged by size:
1993 1873 858
EaeI y'GGCC_r
Cuts at: 2309 2483 2874 2901 4246 2309
Size: 174 391 27 1345 2787
Fragments arranged by size:
2787 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2309 2309
Size: 4724
EarI CTCTTCn'nnn_
Cuts at: 598 2747 2957 598
Size: 2149 210 2365
Fragments arranged by size:
2365 2149 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2932 3242 3388 4534 2932
Size: 310 146 1146 3122
Fragments arranged by size:
3122 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1736 1736
Size: 4724
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 192 655 889 1204 1237 2549 2981 3728 3996
Size: 463 234 315 33 1312 432 747 268
Cuts at: 3996 192
Size: 920
Fragments arranged by size:
1312 920 747 463 432 315 268 234
33
EcoNI CCTnn'n_nnAGG
Cuts at: 1420 1420
Size: 4724
EcoO109I rG'GnC_Cy
Cuts at: 867 1163 1239 1328 1863 867
Size: 296 76 89 535 3728
Fragments arranged by size:
3728 535 296 89 76
EcoRI G'AATT_C
Cuts at: 953 1728 953
Size: 775 3949
EcoRV GAT'ATC
Cuts at: 1653 1653
Size: 4724
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2743 2743
Size: 4724
FspI TGC'GCA
Cuts at: 2505 2505
Size: 4724
HaeII r_GCGC'y
Cuts at: 2158 2406 3428 4407 2158
Size: 248 1022 979 2475
Fragments arranged by size:
2475 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 715 747 1544 1576 2566 2596 2598 2628 715
Size: 32 797 32 990 30 2 30 2811
Fragments arranged by size:
2811 990 797 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1763 1763
Size: 4724
KasI G'GCGC_C
Cuts at: 2154 2402 2154
Size: 248 4476
KpnI G_GTAC'C
Cuts at: 1744 1744
Size: 4724
MluI A'CGCG_T
Cuts at: 4157 4157
Size: 4724
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3395 3579 3395
Size: 184 4540
MscI TGG'CCA
Cuts at: 2485 2485
Size: 4724
MslI CAynn'nnrTG
Cuts at: 566 733 752 790 947 1666 2713 2840 566
Size: 167 19 38 157 719 1047 127 2450
Fragments arranged by size:
2450 1047 719 167 157 127 38 19
MspA1I CmG'CkG
Cuts at: 465 1182 1508 1835 2149 2509 3522 3767 465
Size: 717 326 327 314 360 1013 245 1422
Fragments arranged by size:
1422 1013 717 360 327 326 314 245
NaeI GCC'GGC
Cuts at: 2905 2905
Size: 4724
NarI GG'CG_CC
Cuts at: 2155 2403 2155
Size: 248 4476
NgoMIV G'CCGG_C
Cuts at: 2903 2903
Size: 4724
NsiI A_TGCA'T
Cuts at: 567 567
Size: 4724
NspI r_CATG'y
Cuts at: 569 1023 1377 1558 1777 2277 3184 569
Size: 454 354 181 219 500 907 2109
Fragments arranged by size:
2109 907 500 454 354 219 181
PasI CC'CwG_GG
Cuts at: 703 703
Size: 4724
PciI A'CATG_T
Cuts at: 3180 3180
Size: 4724
PflMI CCAn_nnn'nTGG
Cuts at: 289 633 837 289
Size: 344 204 4176
Fragments arranged by size:
4176 344 204
PmeI GTTT'AAAC
Cuts at: 1723 1723
Size: 4724
PmlI CAC'GTG
Cuts at: 966 1045 966
Size: 79 4645
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3863 3895 3863
Size: 32 4692
PstI C_TGCA'G
Cuts at: 1162 1496 1771 2456 1162
Size: 334 275 685 3430
Fragments arranged by size:
3430 685 334 275
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4724
PvuII CAG'CTG
Cuts at: 465 1182 2149 2509 465
Size: 717 967 360 2680
Fragments arranged by size:
2680 967 717 360
RsrII CG'GwC_CG
Cuts at: 2919 2919
Size: 4724
SacI G_AGCT'C
Cuts at: 1738 1738
Size: 4724
SalI G'TCGA_C
Cuts at: 1761 1761
Size: 4724
SapI GCTCTTCn'nnn_
Cuts at: 598 2747 2957 598
Size: 2149 210 2365
Fragments arranged by size:
2365 2149 210
SbfI CC_TGCA'GG
Cuts at: 1162 1771 1162
Size: 609 4115
ScaI AGT'ACT
Cuts at: 135 4241 135
Size: 4106 618
SfcI C'TryA_G
Cuts at: 33 312 1158 1492 1767 2452 3445 3636 33
Size: 279 846 334 275 685 993 191 1121
Fragments arranged by size:
1121 993 846 685 334 279 275 191
SfoI GGC'GCC
Cuts at: 2156 2404 2156
Size: 248 4476
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4724
SmaI CCC'GGG
Cuts at: 256 1746 256
Size: 1490 3234
SmlI C'TyrA_G
Cuts at: 694 1879 2794 3286 3548 694
Size: 1185 915 492 262 1870
Fragments arranged by size:
1870 1185 915 492 262
SnaBI TAC'GTA
Cuts at: 1716 1716
Size: 4724
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4724
SphI G_CATG'C
Cuts at: 1777 2277 1777
Size: 500 4224
StyI C'CwwG_G
Cuts at: 996 1858 2212 996
Size: 862 354 3508
Fragments arranged by size:
3508 862 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 279 716 2307 2440 2899 3108 279
Size: 437 1591 133 459 209 1895
Fragments arranged by size:
1895 1591 459 437 209 133
TatI w'GTAC_w
Cuts at: 133 821 1640 4239 133
Size: 688 819 2599 618
Fragments arranged by size:
2599 819 688 618
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4241 4273 4241
Size: 32 4692
Tth111I GACn'n_nGTC
Cuts at: 2521 2521
Size: 4724
XbaI T'CTAG_A
Cuts at: 1755 1755
Size: 4724
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 480 1012 2219 480
Size: 532 1207 2985
Fragments arranged by size:
2985 1207 532
XmaI C'CCGG_G
Cuts at: 254 1744 254
Size: 1490 3234
XmnI GAAnn'nnTTC
Cuts at: 380 380
Size: 4724
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NsiI NspI PasI PciI PflMI
PmeI PmlI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbsI BcgI BglI BmtI BsaXI BseRI
BsgI BsiWI BsmBI BssHII BstEII BstZ17I BtgI ClaI
CspCI DraIII FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI PacI PfoI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SrfI SspI StuI SwaI XhoI ZraI