Restriction map for
pF1KSDA1349
(Circular) (Six-base) MAPSORT of: pF1KSDA1349.seq Check: 7596 from: 1 to: 5354
pF1KSDA1349 5354 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 2392 2392
Size: 5354
Acc65I G'GTAC_C
Cuts at: 2370 2370
Size: 5354
AclI AA'CG_TT
Cuts at: 5194 5194
Size: 5354
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 512 1085 1505 2013 2181 2265 3179 3611
Size: 315 573 420 508 168 84 914 432
Cuts at: 3611 4358 4626 197
Size: 747 268 925
Fragments arranged by size:
925 914 747 573 508 432 420 315
268 168 84
AflIII A'CryG_T
Cuts at: 1959 2706 3810 4787 1959
Size: 747 1104 977 2526
Fragments arranged by size:
2526 1104 977 747
AgeI A'CCGG_T
Cuts at: 3742 3742
Size: 5354
AleI CACnn'nnGTG
Cuts at: 980 980
Size: 5354
AlwNI CAG_nnn'CTG
Cuts at: 2659 4226 2659
Size: 1567 3787
ApaLI G'TGCA_C
Cuts at: 1801 4124 1801
Size: 2323 3031
ApoI r'AATT_y
Cuts at: 435 447 453 803 887 1287 1307 1559 1643
Size: 12 6 350 84 400 20 252 84
Cuts at: 1643 2147 2358 4707 4963 435
Size: 504 211 2349 256 826
Fragments arranged by size:
2349 826 504 400 350 256 252 211
84 84 20 12 6
AseI AT'TA_AT
Cuts at: 20 1361 20
Size: 1341 4013
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5354
AvaI C'yCGr_G
Cuts at: 2374 2374
Size: 5354
BamHI G'GATC_C
Cuts at: 2379 2379
Size: 5354
BanI G'GyrC_C
Cuts at: 2370 2784 3032 3067 2370
Size: 414 248 35 4657
Fragments arranged by size:
4657 414 248 35
BanII G_rGCy'C
Cuts at: 134 2368 3398 134
Size: 2234 1030 2090
Fragments arranged by size:
2234 2090 1030
BbeI G_GCGC'C
Cuts at: 2788 3036 2788
Size: 248 5106
BbsI GAAGACnn'nnnn_
Cuts at: 337 1060 337
Size: 723 4631
BciVI GTATCCnnnnn_n'
Cuts at: 3252 4013 5354 3252
Size: 761 1341 3252
Fragments arranged by size:
3252 1341 761
BclI T'GATC_A
Cuts at: 2874 2874
Size: 5354
BfrBI ATG'CAT
Cuts at: 1395 2235 1395
Size: 840 4514
BglII A'GATC_T
Cuts at: 2869 4906 2869
Size: 2037 3317
BlpI GC'TnA_GC
Cuts at: 2466 2466
Size: 5354
Bme1580I G_kGCm'C
Cuts at: 867 1805 2979 3072 4128 867
Size: 938 1174 93 1056 2093
Fragments arranged by size:
2093 1174 1056 938 93
BmrI ACTGGGnnnn_n'
Cuts at: 1241 2165 2566 2647 2982 5126 1241
Size: 924 401 81 335 2144 1469
Fragments arranged by size:
2144 1469 924 401 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3721 3721
Size: 5354
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 749 917 1169 1589 1673 1757 1841 1925 2093
Size: 168 252 420 84 84 84 84 168
Cuts at: 2093 2261 749
Size: 168 3842
Fragments arranged by size:
3842 420 252 168 168 168 84 84
84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 1612 2247 2530 3409 3901 4199 310
Size: 1302 635 283 879 492 298 1465
Fragments arranged by size:
1465 1302 879 635 492 298 283
BsaI GGTCTCn'nnnn_
Cuts at: 5058 5058
Size: 5354
BsaAI yAC'GTr
Cuts at: 2346 2707 2346
Size: 361 4993
BsaBI GATnn'nnATC
Cuts at: 6 363 2868 4905 6
Size: 357 2505 2037 455
Fragments arranged by size:
2505 2037 455 357
BsaHI Gr'CG_yC
Cuts at: 2785 3033 3735 5231 2785
Size: 248 702 1496 2908
Fragments arranged by size:
2908 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2543 3064 3742 4016 4163 4574 2543
Size: 521 678 274 147 411 3323
Fragments arranged by size:
3323 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 2779 3537 4114 4610 2779
Size: 758 577 496 3523
Fragments arranged by size:
3523 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2896 2942 4150 104
Size: 2792 46 1208 1308
Fragments arranged by size:
2792 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1805 2368 3146 3336 4128 1805
Size: 563 778 190 792 3031
Fragments arranged by size:
3031 792 778 563 190
BsmI GAATG_Cn'
Cuts at: 156 928 1270 1354 1397 1606 156
Size: 772 342 84 43 209 3904
Fragments arranged by size:
3904 772 342 209 84 43
Bsp1286I G_dGCh'C
Cuts at: 134 867 1805 2368 2979 3072 3146 3336 3398
Size: 733 938 563 611 93 74 190 62
Cuts at: 3398 4128 134
Size: 730 1360
Fragments arranged by size:
1360 938 733 730 611 563 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4574 4574
Size: 5354
BspHI T'CATG_A
Cuts at: 4530 4530
Size: 5354
BspMI ACCTGCnnnn'nnnn_
Cuts at: 147 2404 2618 2920 3301 147
Size: 2257 214 302 381 2200
Fragments arranged by size:
2257 2200 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3646 3700 5184 5349 50
Size: 3596 54 1484 165 55
Fragments arranged by size:
3596 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 389 613 770 1512 1848 3266 389
Size: 224 157 742 336 1418 2477
Fragments arranged by size:
2477 1418 742 336 224 157
BsrFI r'CCGG_y
Cuts at: 3352 3533 3742 3352
Size: 181 209 4964
Fragments arranged by size:
4964 209 181
BsrGI T'GTAC_A
Cuts at: 1044 1380 1548 1716 2220 2304 1044
Size: 336 168 168 504 84 4094
Fragments arranged by size:
4094 504 336 168 168 84
BssSI C'ACGA_G
Cuts at: 3625 3983 3625
Size: 358 4996
BstAPI GCAn_nnn'nTGC
Cuts at: 2731 2731
Size: 5354
BstBI TT'CG_AA
Cuts at: 110 3715 110
Size: 3605 1749
BstYI r'GATC_y
Cuts at: 1 2379 2846 2869 3204 3450 4462 4548 4560
Size: 2378 467 23 335 246 1012 86 12
Cuts at: 4560 4906 1
Size: 346 449
Fragments arranged by size:
2378 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 125 125
Size: 5354
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1266 3304 3485 3648 1266
Size: 2038 181 163 2972
Fragments arranged by size:
2972 2038 181 163
BtsI GCAGTG_nn'
Cuts at: 2597 2597
Size: 5354
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1074 1109 1494 1529 1830 1865 1074
Size: 35 385 35 301 35 4563
Fragments arranged by size:
4563 385 301 35 35 35
DraI TTT'AAA
Cuts at: 452 1920 2353 452
Size: 1468 433 3453
Fragments arranged by size:
3453 1468 433
DrdI GACnn_nn'nnGTC
Cuts at: 3060 3918 3060
Size: 858 4496
EaeI y'GGCC_r
Cuts at: 2939 3113 3504 3531 4876 2939
Size: 174 391 27 1345 3417
Fragments arranged by size:
3417 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2939 2939
Size: 5354
EarI CTCTTCn'nnn_
Cuts at: 395 1061 1481 3377 3587 395
Size: 666 420 1896 210 2162
Fragments arranged by size:
2162 1896 666 420 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3562 3872 4018 5164 3562
Size: 310 146 1146 3752
Fragments arranged by size:
3752 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2366 2366
Size: 5354
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 512 749 917 1085 1169 1505 1589 1673
Size: 315 237 168 168 84 336 84 84
Cuts at: 1673 1757 1841 1925 2013 2093 2181 2261 2265
Size: 84 84 84 88 80 88 80 4
Cuts at: 2265 3179 3611 4358 4626 197
Size: 914 432 747 268 925
Fragments arranged by size:
925 914 747 432 336 315 268 237
168 168 88 88 84 84 84 84
84 84 80 80 4
EcoNI CCTnn'n_nnAGG
Cuts at: 2031 2031
Size: 5354
EcoO109I rG'GnC_Cy
Cuts at: 1727 2165 2493 1727
Size: 438 328 4588
Fragments arranged by size:
4588 438 328
EcoRI G'AATT_C
Cuts at: 803 887 1307 1559 2147 2358 803
Size: 84 420 252 588 211 3799
Fragments arranged by size:
3799 588 420 252 211 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 542 3373 542
Size: 2831 2523
FspI TGC'GCA
Cuts at: 3135 3135
Size: 5354
HaeII r_GCGC'y
Cuts at: 2788 3036 4058 5037 2788
Size: 248 1022 979 3105
Fragments arranged by size:
3105 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 602 634 3196 3226 3228 3258 602
Size: 32 2562 30 2 30 2698
Fragments arranged by size:
2698 2562 32 30 30 2
HincII GTy'rAC
Cuts at: 2393 2393
Size: 5354
HindIII A'AGCT_T
Cuts at: 932 932
Size: 5354
KasI G'GCGC_C
Cuts at: 2784 3032 2784
Size: 248 5106
KpnI G_GTAC'C
Cuts at: 2374 2374
Size: 5354
MluI A'CGCG_T
Cuts at: 4787 4787
Size: 5354
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4025 4209 4025
Size: 184 5170
MscI TGG'CCA
Cuts at: 3115 3115
Size: 5354
MslI CAynn'nnrTG
Cuts at: 980 1080 1584 1615 1752 1920 3343 3470 980
Size: 100 504 31 137 168 1423 127 2864
Fragments arranged by size:
2864 1423 504 168 137 127 100 31
MspA1I CmG'CkG
Cuts at: 2465 2779 3139 4152 4397 2465
Size: 314 360 1013 245 3422
Fragments arranged by size:
3422 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3535 3535
Size: 5354
NarI GG'CG_CC
Cuts at: 2785 3033 2785
Size: 248 5106
NcoI C'CATG_G
Cuts at: 125 125
Size: 5354
NgoMIV G'CCGG_C
Cuts at: 3533 3533
Size: 5354
NsiI A_TGCA'T
Cuts at: 1397 2237 1397
Size: 840 4514
NspI r_CATG'y
Cuts at: 590 1963 2407 2907 3814 590
Size: 1373 444 500 907 2130
Fragments arranged by size:
2130 1373 907 500 444
PciI A'CATG_T
Cuts at: 1959 3810 1959
Size: 1851 3503
PmeI GTTT'AAAC
Cuts at: 2353 2353
Size: 5354
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4493 4525 4493
Size: 32 5322
PpuMI rG'GwC_Cy
Cuts at: 1727 1727
Size: 5354
PsiI TTA'TAA
Cuts at: 1415 1415
Size: 5354
PstI C_TGCA'G
Cuts at: 2401 3086 2401
Size: 685 4669
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5354
PvuII CAG'CTG
Cuts at: 2779 3139 2779
Size: 360 4994
RsrII CG'GwC_CG
Cuts at: 3549 3549
Size: 5354
SacI G_AGCT'C
Cuts at: 2368 2368
Size: 5354
SalI G'TCGA_C
Cuts at: 2391 2391
Size: 5354
SapI GCTCTTCn'nnn_
Cuts at: 395 3377 3587 395
Size: 2982 210 2162
Fragments arranged by size:
2982 2162 210
SbfI CC_TGCA'GG
Cuts at: 2401 2401
Size: 5354
ScaI AGT'ACT
Cuts at: 4871 4871
Size: 5354
SfcI C'TryA_G
Cuts at: 33 1188 2397 3082 4075 4266 33
Size: 1155 1209 685 993 191 1121
Fragments arranged by size:
1209 1155 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2786 3034 2786
Size: 248 5106
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5354
SmaI CCC'GGG
Cuts at: 2376 2376
Size: 5354
SmlI C'TyrA_G
Cuts at: 325 1627 2226 2509 3424 3916 4178 325
Size: 1302 599 283 915 492 262 1501
Fragments arranged by size:
1501 1302 915 599 492 283 262
SnaBI TAC'GTA
Cuts at: 2346 2346
Size: 5354
SpeI A'CTAG_T
Cuts at: 67 340 67
Size: 273 5081
SphI G_CATG'C
Cuts at: 2407 2907 2407
Size: 500 4854
StuI AGG'CCT
Cuts at: 598 766 1438 598
Size: 168 672 4514
Fragments arranged by size:
4514 672 168
StyI C'CwwG_G
Cuts at: 125 2488 2842 125
Size: 2363 354 2637
Fragments arranged by size:
2637 2363 354
SwaI ATTT'AAAT
Cuts at: 452 1920 452
Size: 1468 3886
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2937 3070 3529 3738 2937
Size: 133 459 209 4553
Fragments arranged by size:
4553 459 209 133
TatI w'GTAC_w
Cuts at: 583 1044 1380 1420 1548 1588 1716 2220 2304
Size: 461 336 40 128 40 128 504 84
Cuts at: 2304 4869 583
Size: 2565 1068
Fragments arranged by size:
2565 1068 504 461 336 128 128 84
40 40
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4871 4903 4871
Size: 32 5322
Tth111I GACn'n_nGTC
Cuts at: 3151 3151
Size: 5354
XbaI T'CTAG_A
Cuts at: 2385 2385
Size: 5354
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2849 2849
Size: 5354
XmaI C'CCGG_G
Cuts at: 2374 2374
Size: 5354
XmnI GAAnn'nnTTC
Cuts at: 368 404 682 1197 1270 1354 1774 368
Size: 36 278 515 73 84 420 3948
Fragments arranged by size:
3948 515 420 278 84 73 36
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PmeI PpiI PpuMI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI DraIII EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI PacI PasI
PflMI PfoI PmlI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SrfI SspI XhoI ZraI