Restriction map for
pF1KSDA1350
(Circular) (Six-base) MAPSORT of: pF1KSDA1350.seq Check: 9061 from: 1 to: 5771
pF1KSDA1350 5771 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 1935 2809 1935
Size: 874 4897
Acc65I G'GTAC_C
Cuts at: 2787 2787
Size: 5771
AclI AA'CG_TT
Cuts at: 5611 5611
Size: 5771
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1695 1753 2423 3596 4028 4775 5043 1695
Size: 58 670 1173 432 747 268 2423
Fragments arranged by size:
2423 1173 747 670 432 268 58
AflIII A'CryG_T
Cuts at: 1893 3123 4227 5204 1893
Size: 1230 1104 977 2460
Fragments arranged by size:
2460 1230 1104 977
AgeI A'CCGG_T
Cuts at: 4159 4159
Size: 5771
AleI CACnn'nnGTG
Cuts at: 809 809
Size: 5771
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2209 2241 2209
Size: 32 5739
AlwNI CAG_nnn'CTG
Cuts at: 2167 3076 4643 2167
Size: 909 1567 3295
Fragments arranged by size:
3295 1567 909
ApaLI G'TGCA_C
Cuts at: 4541 4541
Size: 5771
ApoI r'AATT_y
Cuts at: 239 760 1812 1840 2017 2043 2775 5124 5380
Size: 521 1052 28 177 26 732 2349 256
Cuts at: 5380 239
Size: 630
Fragments arranged by size:
2349 1052 732 630 521 256 177 28
26
AseI AT'TA_AT
Cuts at: 20 20
Size: 5771
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5771
AvaI C'yCGr_G
Cuts at: 2791 2791
Size: 5771
BamHI G'GATC_C
Cuts at: 1404 2796 1404
Size: 1392 4379
BanI G'GyrC_C
Cuts at: 2575 2787 3201 3449 3484 2575
Size: 212 414 248 35 4862
Fragments arranged by size:
4862 414 248 212 35
BanII G_rGCy'C
Cuts at: 2785 3815 2785
Size: 1030 4741
BbeI G_GCGC'C
Cuts at: 3205 3453 3205
Size: 248 5523
BbsI GAAGACnn'nnnn_
Cuts at: 995 1910 2123 995
Size: 915 213 4643
Fragments arranged by size:
4643 915 213
BciVI GTATCCnnnnn_n'
Cuts at: 2673 3669 4430 5771 2673
Size: 996 761 1341 2673
Fragments arranged by size:
2673 1341 996 761
BclI T'GATC_A
Cuts at: 732 846 1614 3291 732
Size: 114 768 1677 3212
Fragments arranged by size:
3212 1677 768 114
BfrBI ATG'CAT
Cuts at: 548 2587 548
Size: 2039 3732
BglII A'GATC_T
Cuts at: 2257 3286 5323 2257
Size: 1029 2037 2705
Fragments arranged by size:
2705 2037 1029
BlpI GC'TnA_GC
Cuts at: 2883 2883
Size: 5771
Bme1580I G_kGCm'C
Cuts at: 3396 3489 4545 3396
Size: 93 1056 4622
Fragments arranged by size:
4622 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2983 3064 3399 5543 2983
Size: 81 335 2144 3211
Fragments arranged by size:
3211 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4138 4138
Size: 5771
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1148 1148
Size: 5771
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2947 3826 4318 4616 2947
Size: 879 492 298 4102
Fragments arranged by size:
4102 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5475 5475
Size: 5771
BsaAI yAC'GTr
Cuts at: 2763 3124 2763
Size: 361 5410
BsaBI GATnn'nnATC
Cuts at: 6 3285 5322 6
Size: 3279 2037 455
Fragments arranged by size:
3279 2037 455
BsaHI Gr'CG_yC
Cuts at: 3202 3450 4152 5648 3202
Size: 248 702 1496 3325
Fragments arranged by size:
3325 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2960 3481 4159 4433 4580 4991 2960
Size: 521 678 274 147 411 3740
Fragments arranged by size:
3740 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 786 816 1747 1777 786
Size: 30 931 30 4780
Fragments arranged by size:
4780 931 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2162 2535 2162
Size: 373 5398
BseYI C'CCAG_C
Cuts at: 3196 3954 4531 5027 3196
Size: 758 577 496 3940
Fragments arranged by size:
3940 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1109 1109
Size: 5771
BsiEI CG_ry'CG
Cuts at: 104 3313 3359 4567 104
Size: 3209 46 1208 1308
Fragments arranged by size:
3209 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 888 2785 3563 3753 4545 888
Size: 1897 778 190 792 2114
Fragments arranged by size:
2114 1897 792 778 190
BsmI GAATG_Cn'
Cuts at: 303 548 2017 303
Size: 245 1469 4057
Fragments arranged by size:
4057 1469 245
Bsp1286I G_dGCh'C
Cuts at: 888 2785 3396 3489 3563 3753 3815 4545 888
Size: 1897 611 93 74 190 62 730 2114
Fragments arranged by size:
2114 1897 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4991 4991
Size: 5771
BspHI T'CATG_A
Cuts at: 1836 4947 1836
Size: 3111 2660
BspMI ACCTGCnnnn'nnnn_
Cuts at: 621 2821 3035 3337 3718 621
Size: 2200 214 302 381 2674
Fragments arranged by size:
2674 2200 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4063 4117 5601 5766 50
Size: 4013 54 1484 165 55
Fragments arranged by size:
4013 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3683 3683
Size: 5771
BsrFI r'CCGG_y
Cuts at: 3769 3950 4159 3769
Size: 181 209 5381
Fragments arranged by size:
5381 209 181
BsrGI T'GTAC_A
Cuts at: 1896 1896
Size: 5771
BssSI C'ACGA_G
Cuts at: 4042 4400 4042
Size: 358 5413
BstAPI GCAn_nnn'nTGC
Cuts at: 554 3148 554
Size: 2594 3177
BstBI TT'CG_AA
Cuts at: 110 4132 110
Size: 4022 1749
BstYI r'GATC_y
Cuts at: 1 1404 2257 2796 3263 3286 3621 3867 4879
Size: 1403 853 539 467 23 335 246 1012
Cuts at: 4879 4965 4977 5323 1
Size: 86 12 346 449
Fragments arranged by size:
1403 1012 853 539 467 449 346 335
246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3721 3902 4065 3721
Size: 181 163 5427
Fragments arranged by size:
5427 181 163
BtsI GCAGTG_nn'
Cuts at: 1617 2739 3014 1617
Size: 1122 275 4374
Fragments arranged by size:
4374 1122 275
ClaI AT'CG_AT
Cuts at: 1658 1658
Size: 5771
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 557 592 2291 2326 557
Size: 35 1699 35 4002
Fragments arranged by size:
4002 1699 35 35
DraI TTT'AAA
Cuts at: 150 713 1038 2397 2770 150
Size: 563 325 1359 373 3151
Fragments arranged by size:
3151 1359 563 373 325
DrdI GACnn_nn'nnGTC
Cuts at: 1355 3477 4335 1355
Size: 2122 858 2791
Fragments arranged by size:
2791 2122 858
EaeI y'GGCC_r
Cuts at: 225 1423 3356 3530 3921 3948 5293 225
Size: 1198 1933 174 391 27 1345 703
Fragments arranged by size:
1933 1345 1198 703 391 174 27
EagI C'GGCC_G
Cuts at: 3356 3356
Size: 5771
EarI CTCTTCn'nnn_
Cuts at: 2089 3794 4004 2089
Size: 1705 210 3856
Fragments arranged by size:
3856 1705 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3979 4289 4435 5581 3979
Size: 310 146 1146 4169
Fragments arranged by size:
4169 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2783 2783
Size: 5771
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1148 1695 1753 2423 3596 4028 4775 5043 1148
Size: 547 58 670 1173 432 747 268 1876
Fragments arranged by size:
1876 1173 747 670 547 432 268 58
EcoNI CCTnn'n_nnAGG
Cuts at: 1549 1549
Size: 5771
EcoO109I rG'GnC_Cy
Cuts at: 2910 2910
Size: 5771
EcoRI G'AATT_C
Cuts at: 1840 2775 1840
Size: 935 4836
EcoRV GAT'ATC
Cuts at: 1683 1683
Size: 5771
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2453 3790 2453
Size: 1337 4434
FspI TGC'GCA
Cuts at: 3552 3552
Size: 5771
HaeII r_GCGC'y
Cuts at: 3205 3453 4475 5454 3205
Size: 248 1022 979 3522
Fragments arranged by size:
3522 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 523 555 738 770 786 818 1146 1178 1747
Size: 32 183 32 16 32 328 32 569
Cuts at: 1747 1764 1779 1796 1955 1987 3613 3643 3645
Size: 17 15 17 159 32 1626 30 2
Cuts at: 3645 3675 523
Size: 30 2619
Fragments arranged by size:
2619 1626 569 328 183 159 32 32
32 32 32 30 30 17 17 16
15 2
HincII GTy'rAC
Cuts at: 2810 2810
Size: 5771
KasI G'GCGC_C
Cuts at: 3201 3449 3201
Size: 248 5523
KpnI G_GTAC'C
Cuts at: 2791 2791
Size: 5771
MfeI C'AATT_G
Cuts at: 278 278
Size: 5771
MluI A'CGCG_T
Cuts at: 5204 5204
Size: 5771
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 110 790 1270 4442 4626 110
Size: 680 480 3172 184 1255
Fragments arranged by size:
3172 1255 680 480 184
MscI TGG'CCA
Cuts at: 227 1425 3532 227
Size: 1198 2107 2466
Fragments arranged by size:
2466 2107 1198
MslI CAynn'nnrTG
Cuts at: 446 809 1898 2471 3760 3887 446
Size: 363 1089 573 1289 127 2330
Fragments arranged by size:
2330 1289 1089 573 363 127
MspA1I CmG'CkG
Cuts at: 641 1256 2882 3196 3556 4569 4814 641
Size: 615 1626 314 360 1013 245 1598
Fragments arranged by size:
1626 1598 1013 615 360 314 245
NaeI GCC'GGC
Cuts at: 3952 3952
Size: 5771
NarI GG'CG_CC
Cuts at: 3202 3450 3202
Size: 248 5523
NdeI CA'TA_TG
Cuts at: 2468 2468
Size: 5771
NgoMIV G'CCGG_C
Cuts at: 3950 3950
Size: 5771
NsiI A_TGCA'T
Cuts at: 550 2589 550
Size: 2039 3732
NspI r_CATG'y
Cuts at: 1897 2824 3324 4231 1897
Size: 927 500 907 3437
Fragments arranged by size:
3437 927 907 500
PciI A'CATG_T
Cuts at: 1893 4227 1893
Size: 2334 3437
PfoI T'CCnGG_A
Cuts at: 1164 1164
Size: 5771
PmeI GTTT'AAAC
Cuts at: 2770 2770
Size: 5771
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4910 4942 4910
Size: 32 5739
PstI C_TGCA'G
Cuts at: 2818 3503 2818
Size: 685 5086
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5771
PvuII CAG'CTG
Cuts at: 641 1256 3196 3556 641
Size: 615 1940 360 2856
Fragments arranged by size:
2856 1940 615 360
RsrII CG'GwC_CG
Cuts at: 3966 3966
Size: 5771
SacI G_AGCT'C
Cuts at: 2785 2785
Size: 5771
SalI G'TCGA_C
Cuts at: 2808 2808
Size: 5771
SapI GCTCTTCn'nnn_
Cuts at: 3794 4004 3794
Size: 210 5561
SbfI CC_TGCA'GG
Cuts at: 2818 2818
Size: 5771
ScaI AGT'ACT
Cuts at: 5288 5288
Size: 5771
SfcI C'TryA_G
Cuts at: 33 201 2814 3499 4492 4683 33
Size: 168 2613 685 993 191 1121
Fragments arranged by size:
2613 1121 993 685 191 168
SfoI GGC'GCC
Cuts at: 3203 3451 3203
Size: 248 5523
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5771
SmaI CCC'GGG
Cuts at: 2793 2793
Size: 5771
SmlI C'TyrA_G
Cuts at: 2926 3841 4333 4595 2926
Size: 915 492 262 4102
Fragments arranged by size:
4102 915 492 262
SnaBI TAC'GTA
Cuts at: 2763 2763
Size: 5771
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5771
SphI G_CATG'C
Cuts at: 2824 3324 2824
Size: 500 5271
SspI AAT'ATT
Cuts at: 777 777
Size: 5771
StuI AGG'CCT
Cuts at: 1544 1544
Size: 5771
StyI C'CwwG_G
Cuts at: 1946 2905 3259 1946
Size: 959 354 4458
Fragments arranged by size:
4458 959 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 331 1112 1862 2234 2255 3354 3487 3946 4155
Size: 781 750 372 21 1099 133 459 209
Cuts at: 4155 331
Size: 1947
Fragments arranged by size:
1947 1099 781 750 459 372 209 133
21
TatI w'GTAC_w
Cuts at: 1896 2431 2461 5286 1896
Size: 535 30 2825 2381
Fragments arranged by size:
2825 2381 535 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5288 5320 5288
Size: 32 5739
Tth111I GACn'n_nGTC
Cuts at: 1593 3568 1593
Size: 1975 3796
XbaI T'CTAG_A
Cuts at: 2802 2802
Size: 5771
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2123 3266 2123
Size: 1143 4628
XmaI C'CCGG_G
Cuts at: 2791 2791
Size: 5771
XmnI GAAnn'nnTTC
Cuts at: 2495 2495
Size: 5771
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NsiI NspI
PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BmtI Bpu10I
BsiWI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
DraIII FseI FspAI HindIII HpaI NcoI NheI NotI
NruI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI SwaI XhoI ZraI