Restriction map for
pF1KSDA1355
(Circular) (Six-base) MAPSORT of: pF1KSDA1355.seq Check: 2426 from: 1 to: 6674
pF1KSDA1355 6674 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 748 748
Size: 6674
AccI GT'mk_AC
Cuts at: 734 1628 2285 3712 734
Size: 894 657 1427 3696
Fragments arranged by size:
3696 1427 894 657
Acc65I G'GTAC_C
Cuts at: 1330 3690 1330
Size: 2360 4314
AclI AA'CG_TT
Cuts at: 6514 6514
Size: 6674
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 500 1893 2557 3485 4499 4931 5678 5946 500
Size: 1393 664 928 1014 432 747 268 1228
Fragments arranged by size:
1393 1228 1014 928 747 664 432 268
AfeI AGC'GCT
Cuts at: 3216 3216
Size: 6674
AflIII A'CryG_T
Cuts at: 4026 5130 6107 4026
Size: 1104 977 4593
Fragments arranged by size:
4593 1104 977
AgeI A'CCGG_T
Cuts at: 5062 5062
Size: 6674
AhdI GACnn_n'nnGTC
Cuts at: 805 1797 3631 805
Size: 992 1834 3848
Fragments arranged by size:
3848 1834 992
AleI CACnn'nnGTG
Cuts at: 1027 1663 3367 1027
Size: 636 1704 4334
Fragments arranged by size:
4334 1704 636
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 634 2320 634
Size: 1686 4988
AlwNI CAG_nnn'CTG
Cuts at: 576 1066 1748 1813 2577 2763 2884 2904 3296
Size: 490 682 65 764 186 121 20 392
Cuts at: 3296 3979 5546 576
Size: 683 1567 1704
Fragments arranged by size:
1704 1567 764 683 682 490 392 186
121 65 20
ApaI G_GGCC'C
Cuts at: 1343 2671 2696 1343
Size: 1328 25 5321
Fragments arranged by size:
5321 1328 25
ApaLI G'TGCA_C
Cuts at: 5444 5444
Size: 6674
ApoI r'AATT_y
Cuts at: 3397 3568 3678 6027 6283 3397
Size: 171 110 2349 256 3788
Fragments arranged by size:
3788 2349 256 171 110
AseI AT'TA_AT
Cuts at: 20 20
Size: 6674
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6674
AvaI C'yCGr_G
Cuts at: 953 1604 1963 2033 3176 3694 953
Size: 651 359 70 1143 518 3933
Fragments arranged by size:
3933 1143 651 518 359 70
BamHI G'GATC_C
Cuts at: 701 962 2055 2548 2589 2619 3699 701
Size: 261 1093 493 41 30 1080 3676
Fragments arranged by size:
3676 1093 1080 493 261 41 30
BanI G'GyrC_C
Cuts at: 134 816 1330 1690 2172 2805 2844 3690 4104
Size: 682 514 360 482 633 39 846 414
Cuts at: 4104 4352 4387 134
Size: 248 35 2421
Fragments arranged by size:
2421 846 682 633 514 482 414 360
248 39 35
BanII G_rGCy'C
Cuts at: 1343 2509 2657 2671 2696 2966 3107 3609 3688
Size: 1166 148 14 25 270 141 502 79
Cuts at: 3688 4718 1343
Size: 1030 3299
Fragments arranged by size:
3299 1166 1030 502 270 148 141 79
25 14
BbeI G_GCGC'C
Cuts at: 4108 4356 4108
Size: 248 6426
BbsI GAAGACnn'nnnn_
Cuts at: 434 488 921 2834 3496 434
Size: 54 433 1913 662 3612
Fragments arranged by size:
3612 1913 662 433 54
BbvCI CC'TCA_GC
Cuts at: 154 875 1059 2321 3268 154
Size: 721 184 1262 947 3560
Fragments arranged by size:
3560 1262 947 721 184
BciVI GTATCCnnnnn_n'
Cuts at: 2988 3060 4572 5333 6674 2988
Size: 72 1512 761 1341 2988
Fragments arranged by size:
2988 1512 1341 761 72
BclI T'GATC_A
Cuts at: 1265 4194 1265
Size: 2929 3745
BglI GCCn_nnn'nGGC
Cuts at: 1609 2111 1609
Size: 502 6172
BglII A'GATC_T
Cuts at: 4189 6226 4189
Size: 2037 4637
BlpI GC'TnA_GC
Cuts at: 3470 3786 3470
Size: 316 6358
Bme1580I G_kGCm'C
Cuts at: 821 1034 1252 1343 1574 1642 2671 2696 2808
Size: 213 218 91 231 68 1029 25 112
Cuts at: 2808 4299 4392 5448 821
Size: 1491 93 1056 2047
Fragments arranged by size:
2047 1491 1056 1029 231 218 213 112
93 91 68 25
BmgBI CAC'GTC
Cuts at: 632 632
Size: 6674
BmrI ACTGGGnnnn_n'
Cuts at: 799 1584 1803 1854 2548 2792 2861 3886 3967
Size: 785 219 51 694 244 69 1025 81
Cuts at: 3967 4302 6446 799
Size: 335 2144 1027
Fragments arranged by size:
2144 1027 1025 785 694 335 244 219
81 69 51
BmtI G_CTAG'C
Cuts at: 1882 1882
Size: 6674
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3202 3408 5041 3202
Size: 206 1633 4835
Fragments arranged by size:
4835 1633 206
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 598 735 2299 2527 2671 2735 2837 2881
Size: 210 137 1564 228 144 64 102 44
Cuts at: 2881 3017 3263 3442 3479 388
Size: 136 246 179 37 3583
Fragments arranged by size:
3583 1564 246 228 210 179 144 137
136 102 64 44 37
Bpu10I CC'TnA_GC
Cuts at: 154 875 1059 2321 2508 2905 3268 154
Size: 721 184 1262 187 397 363 3560
Fragments arranged by size:
3560 1262 721 397 363 187 184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1351 2977 3850 4729 5221 5519 1351
Size: 1626 873 879 492 298 2506
Fragments arranged by size:
2506 1626 879 873 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 421 6378 421
Size: 5957 717
BsaAI yAC'GTr
Cuts at: 369 1632 3666 4027 369
Size: 1263 2034 361 3016
Fragments arranged by size:
3016 2034 1263 361
BsaBI GATnn'nnATC
Cuts at: 6 4188 6225 6
Size: 4182 2037 455
Fragments arranged by size:
4182 2037 455
BsaHI Gr'CG_yC
Cuts at: 4105 4353 5055 6551 4105
Size: 248 702 1496 4228
Fragments arranged by size:
4228 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1932 2272 3863 4384 5062 5336 5483 5894 1932
Size: 340 1591 521 678 274 147 411 2712
Fragments arranged by size:
2712 1591 678 521 411 340 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2357 2387 2879 2909 2357
Size: 30 492 30 6122
Fragments arranged by size:
6122 492 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1454 2974 3369 3514 1454
Size: 1520 395 145 4614
Fragments arranged by size:
4614 1520 395 145
BseYI C'CCAG_C
Cuts at: 223 240 782 998 1033 1089 1377 1635 1893
Size: 17 542 216 35 56 288 258 258
Cuts at: 1893 2123 2245 2560 2605 2756 2810 2965 3088
Size: 230 122 315 45 151 54 155 123
Cuts at: 3088 3296 4099 4857 5434 5930 223
Size: 208 803 758 577 496 967
Fragments arranged by size:
967 803 758 577 542 496 315 288
258 258 230 216 208 155 151 123
122 56 54 45 35 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 703 1293 1943 2474 2960 589
Size: 114 590 650 531 486 4303
Fragments arranged by size:
4303 650 590 531 486 114
BsiEI CG_ry'CG
Cuts at: 104 186 266 270 2477 4216 4262 5470 104
Size: 82 80 4 2207 1739 46 1208 1308
Fragments arranged by size:
2207 1739 1308 1208 82 80 46 4
BsiHKAI G_wGCw'C
Cuts at: 1085 3688 4466 4656 5448 1085
Size: 2603 778 190 792 2311
Fragments arranged by size:
2603 2311 792 778 190
BsmI GAATG_Cn'
Cuts at: 1585 1787 1585
Size: 202 6472
BsmBI CGTCTCn'nnnn_
Cuts at: 2268 2268
Size: 6674
Bsp1286I G_dGCh'C
Cuts at: 821 1034 1085 1252 1343 1574 1642 2509 2657
Size: 213 51 167 91 231 68 867 148
Cuts at: 2657 2671 2696 2808 2966 3107 3609 3688 4299
Size: 14 25 112 158 141 502 79 611
Cuts at: 4299 4392 4466 4656 4718 5448 821
Size: 93 74 190 62 730 2047
Fragments arranged by size:
2047 867 730 611 502 231 213 190
167 158 148 141 112 93 91 79
74 68 62 51 25 14
BspEI T'CCGG_A
Cuts at: 1932 5894 1932
Size: 3962 2712
BspHI T'CATG_A
Cuts at: 5850 5850
Size: 6674
BspMI ACCTGCnnnn'nnnn_
Cuts at: 260 748 3724 3938 4240 4621 260
Size: 488 2976 214 302 381 2313
Fragments arranged by size:
2976 2313 488 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1392 3144 4966 5020 6504 6669 50
Size: 1342 1752 1822 54 1484 165 55
Fragments arranged by size:
1822 1752 1484 1342 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1044 1599 4586 1044
Size: 555 2987 3132
Fragments arranged by size:
3132 2987 555
BsrFI r'CCGG_y
Cuts at: 265 269 1012 2105 2335 2392 2408 4672 4853
Size: 4 743 1093 230 57 16 2264 181
Cuts at: 4853 5062 265
Size: 209 1877
Fragments arranged by size:
2264 1877 1093 743 230 209 181 57
16 4
BssSI C'ACGA_G
Cuts at: 2212 4945 5303 2212
Size: 2733 358 3583
Fragments arranged by size:
3583 2733 358
BstAPI GCAn_nnn'nTGC
Cuts at: 886 2497 4051 886
Size: 1611 1554 3509
Fragments arranged by size:
3509 1611 1554
BstBI TT'CG_AA
Cuts at: 110 5035 110
Size: 4925 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 3202 3532 3202
Size: 330 6344
BstYI r'GATC_y
Cuts at: 1 701 962 2055 2451 2548 2589 2619 3699
Size: 700 261 1093 396 97 41 30 1080
Cuts at: 3699 4166 4189 4524 4770 5782 5868 5880 6226
Size: 467 23 335 246 1012 86 12 346
Cuts at: 6226 1
Size: 449
Fragments arranged by size:
1093 1080 1012 700 467 449 396 346
335 261 246 97 86 41 30 23
12
Bsu36I CC'TnA_GG
Cuts at: 197 3539 197
Size: 3342 3332
BtgI C'CryG_G
Cuts at: 125 606 1566 1670 2005 125
Size: 481 960 104 335 4794
Fragments arranged by size:
4794 960 481 335 104
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1254 4624 4805 4968 1254
Size: 3370 181 163 2960
Fragments arranged by size:
3370 2960 181 163
BtsI GCAGTG_nn'
Cuts at: 1177 1590 1814 2027 3095 3207 3518 3917 1177
Size: 413 224 213 1068 112 311 399 3934
Fragments arranged by size:
3934 1068 413 399 311 224 213 112
DraI TTT'AAA
Cuts at: 3673 3673
Size: 6674
DrdI GACnn_nn'nnGTC
Cuts at: 2481 4380 5238 2481
Size: 1899 858 3917
Fragments arranged by size:
3917 1899 858
EaeI y'GGCC_r
Cuts at: 146 263 267 657 992 1610 1763 2255 2333
Size: 117 4 390 335 618 153 492 78
Cuts at: 2333 2369 2390 3443 4259 4433 4824 4851 6196
Size: 36 21 1053 816 174 391 27 1345
Cuts at: 6196 146
Size: 624
Fragments arranged by size:
1345 1053 816 624 618 492 391 390
335 174 153 117 78 36 27 21
4
EagI C'GGCC_G
Cuts at: 263 267 4259 263
Size: 4 3992 2678
Fragments arranged by size:
3992 2678 4
EarI CTCTTCn'nnn_
Cuts at: 4697 4907 4697
Size: 210 6464
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 694 1437 2197 2363 2434 3571 4882 5192 5338
Size: 743 760 166 71 1137 1311 310 146
Cuts at: 5338 6484 694
Size: 1146 884
Fragments arranged by size:
1311 1146 1137 884 760 743 310 166
146 71
EcoICRI GAG'CTC
Cuts at: 3686 3686
Size: 6674
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 500 598 735 1893 2299 2527 2557 2671
Size: 112 98 137 1158 406 228 30 114
Cuts at: 2671 2735 2837 2881 3017 3263 3442 3479 3485
Size: 64 102 44 136 246 179 37 6
Cuts at: 3485 4499 4931 5678 5946 388
Size: 1014 432 747 268 1116
Fragments arranged by size:
1158 1116 1014 747 432 406 268 246
228 179 137 136 114 112 102 98
64 44 37 30 6
EcoNI CCTnn'n_nnAGG
Cuts at: 658 2185 3278 3562 658
Size: 1527 1093 284 3770
Fragments arranged by size:
3770 1527 1093 284
EcoO109I rG'GnC_Cy
Cuts at: 395 651 799 1212 1340 1458 2638 2667 2692
Size: 256 148 413 128 118 1180 29 25
Cuts at: 2692 2881 3813 395
Size: 189 932 3256
Fragments arranged by size:
3256 1180 932 413 256 189 148 128
118 29 25
EcoRI G'AATT_C
Cuts at: 3397 3568 3678 3397
Size: 171 110 6393
Fragments arranged by size:
6393 171 110
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4693 4693
Size: 6674
FseI GG_CCGG'CC
Cuts at: 269 273 269
Size: 4 6670
FspI TGC'GCA
Cuts at: 4455 4455
Size: 6674
HaeII r_GCGC'y
Cuts at: 770 2442 2682 3006 3218 4108 4356 5378 6357
Size: 1672 240 324 212 890 248 1022 979
Cuts at: 6357 770
Size: 1087
Fragments arranged by size:
1672 1087 1022 979 890 324 248 240
212
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 190 222 399 431 791 823 1730 1762 1783
Size: 32 177 32 360 32 907 32 21
Cuts at: 1783 1815 1974 2006 2357 2389 2540 2572 2611
Size: 32 159 32 351 32 151 32 39
Cuts at: 2611 2643 2879 2911 3617 3649 4516 4546 4548
Size: 32 236 32 706 32 867 30 2
Cuts at: 4548 4578 190
Size: 30 2286
Fragments arranged by size:
2286 907 867 706 360 351 236 177
159 151 39 32 32 32 32 32
32 32 32 32 32 32 30 30
21 2
HincII GTy'rAC
Cuts at: 3713 3713
Size: 6674
KasI G'GCGC_C
Cuts at: 4104 4352 4104
Size: 248 6426
KpnI G_GTAC'C
Cuts at: 1334 3694 1334
Size: 2360 4314
MluI A'CGCG_T
Cuts at: 6107 6107
Size: 6674
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 542 2497 5345 5529 542
Size: 1955 2848 184 1687
Fragments arranged by size:
2848 1955 1687 184
MscI TGG'CCA
Cuts at: 659 994 1612 1765 3445 4435 659
Size: 335 618 153 1680 990 2898
Fragments arranged by size:
2898 1680 990 618 335 153
MslI CAynn'nnrTG
Cuts at: 814 1027 1135 1663 3367 4663 4790 814
Size: 213 108 528 1704 1296 127 2698
Fragments arranged by size:
2698 1704 1296 528 213 127 108
MspA1I CmG'CkG
Cuts at: 786 905 1154 1193 1221 1957 2007 2304 2488
Size: 119 249 39 28 736 50 297 184
Cuts at: 2488 3296 3548 3785 4099 4459 5472 5717 786
Size: 808 252 237 314 360 1013 245 1743
Fragments arranged by size:
1743 1013 808 736 360 314 297 252
249 245 237 184 119 50 39 28
NaeI GCC'GGC
Cuts at: 267 271 1014 2107 2337 2394 4855 267
Size: 4 743 1093 230 57 2461 2086
Fragments arranged by size:
2461 2086 1093 743 230 57 4
NarI GG'CG_CC
Cuts at: 4105 4353 4105
Size: 248 6426
NcoI C'CATG_G
Cuts at: 125 125
Size: 6674
NgoMIV G'CCGG_C
Cuts at: 265 269 1012 2105 2335 2392 4853 265
Size: 4 743 1093 230 57 2461 2086
Fragments arranged by size:
2461 2086 1093 743 230 57 4
NheI G'CTAG_C
Cuts at: 1878 1878
Size: 6674
NspI r_CATG'y
Cuts at: 286 1140 3337 3727 4227 5134 286
Size: 854 2197 390 500 907 1826
Fragments arranged by size:
2197 1826 907 854 500 390
PasI CC'CwG_GG
Cuts at: 1245 1275 1293 2118 3056 3403 1245
Size: 30 18 825 938 347 4516
Fragments arranged by size:
4516 938 825 347 30 18
PciI A'CATG_T
Cuts at: 5130 5130
Size: 6674
PflMI CCAn_nnn'nTGG
Cuts at: 131 2763 2884 3296 131
Size: 2632 121 412 3509
Fragments arranged by size:
3509 2632 412 121
PfoI T'CCnGG_A
Cuts at: 461 539 911 2883 3279 461
Size: 78 372 1972 396 3856
Fragments arranged by size:
3856 1972 396 372 78
PmeI GTTT'AAAC
Cuts at: 3673 3673
Size: 6674
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5813 5845 5813
Size: 32 6642
PpuMI rG'GwC_Cy
Cuts at: 651 799 1458 2638 2881 651
Size: 148 659 1180 243 4444
Fragments arranged by size:
4444 1180 659 243 148
PspOMI G'GGCC_C
Cuts at: 1339 2667 2692 1339
Size: 1328 25 5321
Fragments arranged by size:
5321 1328 25
PspXI vC'TCGA_Gb
Cuts at: 3176 3176
Size: 6674
PstI C_TGCA'G
Cuts at: 389 950 1220 1736 1850 3102 3255 3721 4406
Size: 561 270 516 114 1252 153 466 685
Cuts at: 4406 389
Size: 2657
Fragments arranged by size:
2657 1252 685 561 516 466 270 153
114
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6674
PvuII CAG'CTG
Cuts at: 786 905 1193 1221 1957 2304 2488 3296 4099
Size: 119 288 28 736 347 184 808 803
Cuts at: 4099 4459 786
Size: 360 3001
Fragments arranged by size:
3001 808 803 736 360 347 288 184
119 28
RsrII CG'GwC_CG
Cuts at: 4869 4869
Size: 6674
SacI G_AGCT'C
Cuts at: 3688 3688
Size: 6674
SacII CC_GC'GG
Cuts at: 2008 2008
Size: 6674
SalI G'TCGA_C
Cuts at: 3711 3711
Size: 6674
SanDI GG'GwC_CC
Cuts at: 1458 2638 1458
Size: 1180 5494
SapI GCTCTTCn'nnn_
Cuts at: 4697 4907 4697
Size: 210 6464
SbfI CC_TGCA'GG
Cuts at: 3721 3721
Size: 6674
ScaI AGT'ACT
Cuts at: 6191 6191
Size: 6674
SfcI C'TryA_G
Cuts at: 33 385 900 946 1216 1732 1846 2781 3098
Size: 352 515 46 270 516 114 935 317
Cuts at: 3098 3251 3717 4402 5395 5586 33
Size: 153 466 685 993 191 1121
Fragments arranged by size:
1121 993 935 685 516 515 466 352
317 270 191 153 114 46
SfiI GGCCn_nnn'nGGCC
Cuts at: 1609 1609
Size: 6674
SfoI GGC'GCC
Cuts at: 4106 4354 4106
Size: 248 6426
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6674
SmaI CCC'GGG
Cuts at: 955 1965 2035 3696 955
Size: 1010 70 1661 3933
Fragments arranged by size:
3933 1661 1010 70
SmlI C'TyrA_G
Cuts at: 1366 2956 3176 3829 4744 5236 5498 1366
Size: 1590 220 653 915 492 262 2542
Fragments arranged by size:
2542 1590 915 653 492 262 220
SnaBI TAC'GTA
Cuts at: 3666 3666
Size: 6674
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6674
SphI G_CATG'C
Cuts at: 1140 3727 4227 1140
Size: 2587 500 3587
Fragments arranged by size:
3587 2587 500
SspI AAT'ATT
Cuts at: 1408 1408
Size: 6674
StuI AGG'CCT
Cuts at: 2180 3158 2180
Size: 978 5696
StyI C'CwwG_G
Cuts at: 125 654 1199 1397 1452 1484 1556 1685 1802
Size: 529 545 198 55 32 72 129 117
Cuts at: 1802 3808 4162 125
Size: 2006 354 2637
Fragments arranged by size:
2637 2006 545 529 354 198 129 117
72 55 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 495 787 796 1012 1785 1793 1873 2880 4257
Size: 292 9 216 773 8 80 1007 1377
Cuts at: 4257 4390 4849 5058 495
Size: 133 459 209 2111
Fragments arranged by size:
2111 1377 1007 773 459 292 216 209
133 80 9 8
TatI w'GTAC_w
Cuts at: 6189 6189
Size: 6674
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6191 6223 6191
Size: 32 6642
Tth111I GACn'n_nGTC
Cuts at: 4471 4471
Size: 6674
XbaI T'CTAG_A
Cuts at: 3705 3705
Size: 6674
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1596 1668 2274 4169 1596
Size: 72 606 1895 4101
Fragments arranged by size:
4101 1895 606 72
XhoI C'TCGA_G
Cuts at: 3176 3176
Size: 6674
XmaI C'CCGG_G
Cuts at: 953 1963 2033 3694 953
Size: 1010 70 1661 3933
Fragments arranged by size:
3933 1661 1010 70
XmnI GAAnn'nnTTC
Cuts at: 1940 3065 1940
Size: 1125 5549
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AflII AloI AscI AvrII BaeI BcgI BfrBI
BsiWI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII
EcoRV FspAI HindIII HpaI MfeI NdeI NotI NruI
NsiI PacI PmlI PshAI PsiI PsrI SexAI SgrAI
SrfI SwaI ZraI