Restriction map for
pF1KSDA1360
(Circular) (Six-base) MAPSORT of: pF1KSDA1360.seq Check: 6579 from: 1 to: 5519
pF1KSDA1360 5519 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 1209 2557 1209
Size: 1348 4171
Acc65I G'GTAC_C
Cuts at: 864 2535 864
Size: 1671 3848
AclI AA'CG_TT
Cuts at: 5359 5359
Size: 5519
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 215 733 824 2048 3344 3776 4523 4791
Size: 46 518 91 1224 1296 432 747 268
Cuts at: 4791 169
Size: 897
Fragments arranged by size:
1296 1224 897 747 518 432 268 91
46
AflIII A'CryG_T
Cuts at: 615 2871 3975 4952 615
Size: 2256 1104 977 1182
Fragments arranged by size:
2256 1182 1104 977
AgeI A'CCGG_T
Cuts at: 3907 3907
Size: 5519
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1328 1360 1328
Size: 32 5487
AlwNI CAG_nnn'CTG
Cuts at: 1806 2824 4391 1806
Size: 1018 1567 2934
Fragments arranged by size:
2934 1567 1018
ApaLI G'TGCA_C
Cuts at: 1837 4289 1837
Size: 2452 3067
ApoI r'AATT_y
Cuts at: 343 369 821 844 1239 1322 1355 2022 2523
Size: 26 452 23 395 83 33 667 501
Cuts at: 2523 4872 5128 343
Size: 2349 256 734
Fragments arranged by size:
2349 734 667 501 452 395 256 83
33 26 23
AseI AT'TA_AT
Cuts at: 20 931 20
Size: 911 4608
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5519
AvaI C'yCGr_G
Cuts at: 2539 2539
Size: 5519
BamHI G'GATC_C
Cuts at: 2544 2544
Size: 5519
BanI G'GyrC_C
Cuts at: 864 2535 2949 3197 3232 864
Size: 1671 414 248 35 3151
Fragments arranged by size:
3151 1671 414 248 35
BanII G_rGCy'C
Cuts at: 1121 2533 3563 1121
Size: 1412 1030 3077
Fragments arranged by size:
3077 1412 1030
BbeI G_GCGC'C
Cuts at: 2953 3201 2953
Size: 248 5271
BbsI GAAGACnn'nnnn_
Cuts at: 379 379
Size: 5519
BbvCI CC'TCA_GC
Cuts at: 899 899
Size: 5519
BciVI GTATCCnnnnn_n'
Cuts at: 3417 4178 5519 3417
Size: 761 1341 3417
Fragments arranged by size:
3417 1341 761
BclI T'GATC_A
Cuts at: 3039 3039
Size: 5519
BglII A'GATC_T
Cuts at: 2496 3034 5071 2496
Size: 538 2037 2944
Fragments arranged by size:
2944 2037 538
BlpI GC'TnA_GC
Cuts at: 2631 2631
Size: 5519
Bme1580I G_kGCm'C
Cuts at: 1841 3144 3237 4293 1841
Size: 1303 93 1056 3067
Fragments arranged by size:
3067 1303 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2731 2812 3147 5291 2731
Size: 81 335 2144 2959
Fragments arranged by size:
2959 2144 335 81
BmtI G_CTAG'C
Cuts at: 986 2483 986
Size: 1497 4022
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2204 3886 2204
Size: 1682 3837
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 152 1570 152
Size: 1418 4101
Bpu10I CC'TnA_GC
Cuts at: 899 1254 899
Size: 355 5164
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2318 2695 3574 4066 4364 2318
Size: 377 879 492 298 3473
Fragments arranged by size:
3473 879 492 377 298
BsaI GGTCTCn'nnnn_
Cuts at: 5223 5223
Size: 5519
BsaAI yAC'GTr
Cuts at: 2511 2872 2511
Size: 361 5158
BsaBI GATnn'nnATC
Cuts at: 6 662 3033 5070 6
Size: 656 2371 2037 455
Fragments arranged by size:
2371 2037 656 455
BsaHI Gr'CG_yC
Cuts at: 2950 3198 3900 5396 2950
Size: 248 702 1496 3073
Fragments arranged by size:
3073 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2708 3229 3907 4181 4328 4739 2708
Size: 521 678 274 147 411 3488
Fragments arranged by size:
3488 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1633 2321 1633
Size: 688 4831
BseYI C'CCAG_C
Cuts at: 2944 3702 4279 4775 2944
Size: 758 577 496 3688
Fragments arranged by size:
3688 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 722 835 722
Size: 113 5406
BsiEI CG_ry'CG
Cuts at: 104 3061 3107 4315 104
Size: 2957 46 1208 1308
Fragments arranged by size:
2957 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1089 1121 1841 2533 3311 3501 4293 1089
Size: 32 720 692 778 190 792 2315
Fragments arranged by size:
2315 792 778 720 692 190 32
BsmI GAATG_Cn'
Cuts at: 910 910
Size: 5519
Bsp1286I G_dGCh'C
Cuts at: 1089 1121 1841 2533 3144 3237 3311 3501 3563
Size: 32 720 692 611 93 74 190 62
Cuts at: 3563 4293 1089
Size: 730 2315
Fragments arranged by size:
2315 730 720 692 611 190 93 74
62 32
BspEI T'CCGG_A
Cuts at: 4739 4739
Size: 5519
BspHI T'CATG_A
Cuts at: 4695 4695
Size: 5519
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1347 2569 2783 3085 3466 1347
Size: 1222 214 302 381 3400
Fragments arranged by size:
3400 1222 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3811 3865 5349 5514 50
Size: 3761 54 1484 165 55
Fragments arranged by size:
3761 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2490 3431 2490
Size: 941 4578
BsrFI r'CCGG_y
Cuts at: 3517 3698 3907 3517
Size: 181 209 5129
Fragments arranged by size:
5129 209 181
BssSI C'ACGA_G
Cuts at: 3790 4148 3790
Size: 358 5161
BstAPI GCAn_nnn'nTGC
Cuts at: 2896 2896
Size: 5519
BstBI TT'CG_AA
Cuts at: 110 3880 110
Size: 3770 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 2309 2309
Size: 5519
BstYI r'GATC_y
Cuts at: 1 574 1109 2496 2544 3011 3034 3369 3615
Size: 573 535 1387 48 467 23 335 246
Cuts at: 3615 4627 4713 4725 5071 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1387 1012 573 535 467 449 346 335
246 86 48 23 12
Bsu36I CC'TnA_GG
Cuts at: 599 599
Size: 5519
BtgI C'CryG_G
Cuts at: 1073 1973 1073
Size: 900 4619
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3469 3650 3813 3469
Size: 181 163 5175
Fragments arranged by size:
5175 181 163
BtsI GCAGTG_nn'
Cuts at: 225 709 1704 2762 225
Size: 484 995 1058 2982
Fragments arranged by size:
2982 1058 995 484
DraI TTT'AAA
Cuts at: 2492 2518 2492
Size: 26 5493
DrdI GACnn_nn'nnGTC
Cuts at: 3225 4083 3225
Size: 858 4661
EaeI y'GGCC_r
Cuts at: 1418 3104 3278 3669 3696 5041 1418
Size: 1686 174 391 27 1345 1896
Fragments arranged by size:
1896 1686 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3104 3104
Size: 5519
EarI CTCTTCn'nnn_
Cuts at: 3542 3752 3542
Size: 210 5309
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3727 4037 4183 5329 3727
Size: 310 146 1146 3917
Fragments arranged by size:
3917 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1119 2531 1119
Size: 1412 4107
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 152 169 215 733 824 1570 2048 3344 3776
Size: 17 46 518 91 746 478 1296 432
Cuts at: 3776 4523 4791 152
Size: 747 268 880
Fragments arranged by size:
1296 880 747 746 518 478 432 268
91 46 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2333 2333
Size: 5519
EcoO109I rG'GnC_Cy
Cuts at: 363 2658 363
Size: 2295 3224
EcoRI G'AATT_C
Cuts at: 2523 2523
Size: 5519
EcoRV GAT'ATC
Cuts at: 1302 1302
Size: 5519
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 699 1531 3538 699
Size: 832 2007 2680
Fragments arranged by size:
2680 2007 832
FspI TGC'GCA
Cuts at: 3300 3300
Size: 5519
HaeII r_GCGC'y
Cuts at: 2953 3201 4223 5202 2953
Size: 248 1022 979 3270
Fragments arranged by size:
3270 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1150 1182 1885 1917 3361 3391 3393 3423 1150
Size: 32 703 32 1444 30 2 30 3246
Fragments arranged by size:
3246 1444 703 32 32 30 30 2
HincII GTy'rAC
Cuts at: 516 2558 516
Size: 2042 3477
KasI G'GCGC_C
Cuts at: 2949 3197 2949
Size: 248 5271
KpnI G_GTAC'C
Cuts at: 868 2539 868
Size: 1671 3848
MluI A'CGCG_T
Cuts at: 4952 4952
Size: 5519
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 533 667 1162 2103 2368 4190 4374 533
Size: 134 495 941 265 1822 184 1678
Fragments arranged by size:
1822 1678 941 495 265 184 134
MscI TGG'CCA
Cuts at: 3280 3280
Size: 5519
MslI CAynn'nnrTG
Cuts at: 218 1248 1888 1984 2227 3508 3635 218
Size: 1030 640 96 243 1281 127 2102
Fragments arranged by size:
2102 1281 1030 640 243 127 96
MspA1I CmG'CkG
Cuts at: 1416 2630 2944 3304 4317 4562 1416
Size: 1214 314 360 1013 245 2373
Fragments arranged by size:
2373 1214 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3700 3700
Size: 5519
NarI GG'CG_CC
Cuts at: 2950 3198 2950
Size: 248 5271
NcoI C'CATG_G
Cuts at: 1073 1973 1073
Size: 900 4619
NgoMIV G'CCGG_C
Cuts at: 3698 3698
Size: 5519
NheI G'CTAG_C
Cuts at: 982 2479 982
Size: 1497 4022
NspI r_CATG'y
Cuts at: 619 2079 2572 3072 3979 619
Size: 1460 493 500 907 2159
Fragments arranged by size:
2159 1460 907 500 493
PciI A'CATG_T
Cuts at: 615 3975 615
Size: 3360 2159
PfoI T'CCnGG_A
Cuts at: 1112 1112
Size: 5519
PmeI GTTT'AAAC
Cuts at: 2518 2518
Size: 5519
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4658 4690 4658
Size: 32 5487
PpuMI rG'GwC_Cy
Cuts at: 363 363
Size: 5519
PsiI TTA'TAA
Cuts at: 149 149
Size: 5519
PstI C_TGCA'G
Cuts at: 2566 3251 2566
Size: 685 4834
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5519
PvuII CAG'CTG
Cuts at: 1416 2944 3304 1416
Size: 1528 360 3631
Fragments arranged by size:
3631 1528 360
RsrII CG'GwC_CG
Cuts at: 3714 3714
Size: 5519
SacI G_AGCT'C
Cuts at: 1121 2533 1121
Size: 1412 4107
SalI G'TCGA_C
Cuts at: 2556 2556
Size: 5519
SanDI GG'GwC_CC
Cuts at: 363 363
Size: 5519
SapI GCTCTTCn'nnn_
Cuts at: 3542 3752 3542
Size: 210 5309
SbfI CC_TGCA'GG
Cuts at: 2566 2566
Size: 5519
ScaI AGT'ACT
Cuts at: 1953 5036 1953
Size: 3083 2436
SfcI C'TryA_G
Cuts at: 33 2103 2562 3247 4240 4431 33
Size: 2070 459 685 993 191 1121
Fragments arranged by size:
2070 1121 993 685 459 191
SfoI GGC'GCC
Cuts at: 2951 3199 2951
Size: 248 5271
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5519
SmaI CCC'GGG
Cuts at: 2541 2541
Size: 5519
SmlI C'TyrA_G
Cuts at: 2333 2674 3589 4081 4343 2333
Size: 341 915 492 262 3509
Fragments arranged by size:
3509 915 492 341 262
SnaBI TAC'GTA
Cuts at: 2511 2511
Size: 5519
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5519
SphI G_CATG'C
Cuts at: 2572 3072 2572
Size: 500 5019
SspI AAT'ATT
Cuts at: 403 718 1638 403
Size: 315 920 4284
Fragments arranged by size:
4284 920 315
StyI C'CwwG_G
Cuts at: 1073 1331 1973 2653 3007 1073
Size: 258 642 680 354 3585
Fragments arranged by size:
3585 680 642 354 258
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3102 3235 3694 3903 3102
Size: 133 459 209 4718
Fragments arranged by size:
4718 459 209 133
TatI w'GTAC_w
Cuts at: 1290 1375 1951 5034 1290
Size: 85 576 3083 1775
Fragments arranged by size:
3083 1775 576 85
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1950 1982 5036 5068 1950
Size: 32 3054 32 2401
Fragments arranged by size:
3054 2401 32 32
Tth111I GACn'n_nGTC
Cuts at: 3316 3316
Size: 5519
XbaI T'CTAG_A
Cuts at: 2550 2550
Size: 5519
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1413 2139 3014 1413
Size: 726 875 3918
Fragments arranged by size:
3918 875 726
XmaI C'CCGG_G
Cuts at: 2539 2539
Size: 5519
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PciI
PfoI PmeI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BsaXI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI
DraIII FseI FspAI HindIII HpaI MfeI NdeI NotI
NruI NsiI PacI PasI PflMI PmlI PshAI PspOMI
PspXI PsrI SacII SexAI SfiI SgrAI SrfI StuI
SwaI XhoI XmnI ZraI