Restriction map for
pF1KSDA1368
(Circular) (Six-base) MAPSORT of: pF1KSDA1368.seq Check: 3304 from: 1 to: 6278
pF1KSDA1368 6278 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AatII G_ACGT'C
Cuts at: 949 949
Size: 6278
AccI GT'mk_AC
Cuts at: 437 2186 3316 437
Size: 1749 1130 3399
Fragments arranged by size:
3399 1749 1130
Acc65I G'GTAC_C
Cuts at: 3294 3294
Size: 6278
AclI AA'CG_TT
Cuts at: 644 1066 6118 644
Size: 422 5052 804
Fragments arranged by size:
5052 804 422
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 816 976 3196 4103 4535 5282 5550 816
Size: 160 2220 907 432 747 268 1544
Fragments arranged by size:
2220 1544 907 747 432 268 160
AflIII A'CryG_T
Cuts at: 3630 4734 5711 3630
Size: 1104 977 4197
Fragments arranged by size:
4197 1104 977
AgeI A'CCGG_T
Cuts at: 2869 4666 2869
Size: 1797 4481
AlwNI CAG_nnn'CTG
Cuts at: 688 982 1195 1411 3583 5150 688
Size: 294 213 216 2172 1567 1816
Fragments arranged by size:
2172 1816 1567 294 216 213
ApaLI G'TGCA_C
Cuts at: 3088 5048 3088
Size: 1960 4318
ApoI r'AATT_y
Cuts at: 389 598 3282 5631 5887 389
Size: 209 2684 2349 256 780
Fragments arranged by size:
2684 2349 780 256 209
AseI AT'TA_AT
Cuts at: 20 20
Size: 6278
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6278
AvaI C'yCGr_G
Cuts at: 2172 2847 3126 3298 2172
Size: 675 279 172 5152
Fragments arranged by size:
5152 675 279 172
BamHI G'GATC_C
Cuts at: 3303 3303
Size: 6278
BanI G'GyrC_C
Cuts at: 1744 3294 3708 3956 3991 1744
Size: 1550 414 248 35 4031
Fragments arranged by size:
4031 1550 414 248 35
BanII G_rGCy'C
Cuts at: 2249 2269 2563 3292 4322 2249
Size: 20 294 729 1030 4205
Fragments arranged by size:
4205 1030 729 294 20
BbeI G_GCGC'C
Cuts at: 3712 3960 3712
Size: 248 6030
BbsI GAAGACnn'nnnn_
Cuts at: 1580 2036 2160 1580
Size: 456 124 5698
Fragments arranged by size:
5698 456 124
BciVI GTATCCnnnnn_n'
Cuts at: 248 567 4176 4937 6278 248
Size: 319 3609 761 1341 248
Fragments arranged by size:
3609 1341 761 319 248
BclI T'GATC_A
Cuts at: 311 2199 3798 311
Size: 1888 1599 2791
Fragments arranged by size:
2791 1888 1599
BfrBI ATG'CAT
Cuts at: 517 517
Size: 6278
BglI GCCn_nnn'nGGC
Cuts at: 2698 2698
Size: 6278
BglII A'GATC_T
Cuts at: 3793 5830 3793
Size: 2037 4241
BlpI GC'TnA_GC
Cuts at: 2290 3390 2290
Size: 1100 5178
Bme1580I G_kGCm'C
Cuts at: 1334 3092 3903 3996 5052 1334
Size: 1758 811 93 1056 2560
Fragments arranged by size:
2560 1758 1056 811 93
BmgBI CAC'GTC
Cuts at: 2217 2217
Size: 6278
BmrI ACTGGGnnnn_n'
Cuts at: 1154 3490 3571 3906 6050 1154
Size: 2336 81 335 2144 1382
Fragments arranged by size:
2336 2144 1382 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4645 4645
Size: 6278
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 952 997 1004 1543 1692 2725 2746 3003 952
Size: 45 7 539 149 1033 21 257 4227
Fragments arranged by size:
4227 1033 539 257 149 45 21 7
Bpu10I CC'TnA_GC
Cuts at: 890 1211 890
Size: 321 5957
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1940 3454 4333 4825 5123 1940
Size: 1514 879 492 298 3095
Fragments arranged by size:
3095 1514 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1706 5982 1706
Size: 4276 2002
BsaAI yAC'GTr
Cuts at: 2664 3270 3631 2664
Size: 606 361 5311
Fragments arranged by size:
5311 606 361
BsaBI GATnn'nnATC
Cuts at: 6 310 1036 3792 5829 6
Size: 304 726 2756 2037 455
Fragments arranged by size:
2756 2037 726 455 304
BsaHI Gr'CG_yC
Cuts at: 946 3709 3957 4659 6155 946
Size: 2763 248 702 1496 1069
Fragments arranged by size:
2763 1496 1069 702 248
BsaWI w'CCGG_w
Cuts at: 240 719 2869 3467 3988 4666 4940 5087 5498
Size: 479 2150 598 521 678 274 147 411
Cuts at: 5498 240
Size: 1020
Fragments arranged by size:
2150 1020 678 598 521 479 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 925 955 2980 3010 925
Size: 30 2025 30 4193
Fragments arranged by size:
4193 2025 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1540 1856 2892 2943 2988 1540
Size: 316 1036 51 45 4830
Fragments arranged by size:
4830 1036 316 51 45
BseYI C'CCAG_C
Cuts at: 172 1411 1927 2485 2602 2614 3098 3136 3703
Size: 1239 516 558 117 12 484 38 567
Cuts at: 3703 4461 5038 5534 172
Size: 758 577 496 916
Fragments arranged by size:
1239 916 758 577 567 558 516 496
484 117 38 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2248 2520 2248
Size: 272 6006
BsiEI CG_ry'CG
Cuts at: 104 3820 3866 5074 104
Size: 3716 46 1208 1308
Fragments arranged by size:
3716 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2249 3092 3292 4070 4260 5052 2249
Size: 843 200 778 190 792 3475
Fragments arranged by size:
3475 843 792 778 200 190
BsiWI C'GTAC_G
Cuts at: 3181 3181
Size: 6278
BsmI GAATG_Cn'
Cuts at: 1908 1988 1908
Size: 80 6198
Bsp1286I G_dGCh'C
Cuts at: 1334 2249 2269 2563 3092 3292 3903 3996 4070
Size: 915 20 294 529 200 611 93 74
Cuts at: 4070 4260 4322 5052 1334
Size: 190 62 730 2560
Fragments arranged by size:
2560 915 730 611 529 294 200 190
93 74 62 20
BspEI T'CCGG_A
Cuts at: 5498 5498
Size: 6278
BspHI T'CATG_A
Cuts at: 314 5454 314
Size: 5140 1138
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2591 3328 3542 3844 4225 2591
Size: 737 214 302 381 4644
Fragments arranged by size:
4644 737 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1314 4570 4624 6108 6273 50
Size: 1264 3256 54 1484 165 55
Fragments arranged by size:
3256 1484 1264 165 55 54
BsrDI GCAATG_nn'
Cuts at: 332 1127 2132 4190 332
Size: 795 1005 2058 2420
Fragments arranged by size:
2420 2058 1005 795
BsrFI r'CCGG_y
Cuts at: 1664 2869 4276 4457 4666 1664
Size: 1205 1407 181 209 3276
Fragments arranged by size:
3276 1407 1205 209 181
BsrGI T'GTAC_A
Cuts at: 3262 3262
Size: 6278
BssHII G'CGCG_C
Cuts at: 961 961
Size: 6278
BssSI C'ACGA_G
Cuts at: 2952 4549 4907 2952
Size: 1597 358 4323
Fragments arranged by size:
4323 1597 358
BstAPI GCAn_nnn'nTGC
Cuts at: 655 1108 3655 655
Size: 453 2547 3278
Fragments arranged by size:
3278 2547 453
BstBI TT'CG_AA
Cuts at: 110 4639 110
Size: 4529 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 2622 3034 3106 2622
Size: 412 72 5794
Fragments arranged by size:
5794 412 72
BstYI r'GATC_y
Cuts at: 1 916 3303 3770 3793 4128 4374 5386 5472
Size: 915 2387 467 23 335 246 1012 86
Cuts at: 5472 5484 5830 1
Size: 12 346 449
Fragments arranged by size:
2387 1012 915 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 800 860 1351 2490 2555 2576 2772 800
Size: 60 491 1139 65 21 196 4306
Fragments arranged by size:
4306 1139 491 196 65 60 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4228 4409 4572 4228
Size: 181 163 5934
Fragments arranged by size:
5934 181 163
BtsI GCAGTG_nn'
Cuts at: 157 851 1334 1403 2045 3521 157
Size: 694 483 69 642 1476 2914
Fragments arranged by size:
2914 1476 694 642 483 69
DraI TTT'AAA
Cuts at: 3277 3277
Size: 6278
DraIII CAC_nnn'GTG
Cuts at: 1434 1434
Size: 6278
DrdI GACnn_nn'nnGTC
Cuts at: 3984 4842 3984
Size: 858 5420
EaeI y'GGCC_r
Cuts at: 611 1478 1662 2129 2390 3108 3863 4037 4428
Size: 867 184 467 261 718 755 174 391
Cuts at: 4428 4455 5800 611
Size: 27 1345 1089
Fragments arranged by size:
1345 1089 867 755 718 467 391 261
184 174 27
EagI C'GGCC_G
Cuts at: 3863 3863
Size: 6278
EarI CTCTTCn'nnn_
Cuts at: 2910 4301 4511 2910
Size: 1391 210 4677
Fragments arranged by size:
4677 1391 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4486 4796 4942 6088 4486
Size: 310 146 1146 4676
Fragments arranged by size:
4676 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2247 3290 2247
Size: 1043 5235
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 816 952 976 997 1004 1543 1692 2725 2746
Size: 136 24 21 7 539 149 1033 21
Cuts at: 2746 3003 3196 4103 4535 5282 5550 816
Size: 257 193 907 432 747 268 1544
Fragments arranged by size:
1544 1033 907 747 539 432 268 257
193 149 136 24 21 21 7
EcoO109I rG'GnC_Cy
Cuts at: 2842 3417 2842
Size: 575 5703
EcoRI G'AATT_C
Cuts at: 598 3282 598
Size: 2684 3594
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 981 4297 981
Size: 3316 2962
FspI TGC'GCA
Cuts at: 4059 4059
Size: 6278
HaeII r_GCGC'y
Cuts at: 2704 3712 3960 4982 5961 2704
Size: 1008 248 1022 979 3021
Fragments arranged by size:
3021 1022 1008 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 803 835 2299 2331 4120 4150 4152 4182 803
Size: 32 1464 32 1789 30 2 30 2899
Fragments arranged by size:
2899 1789 1464 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2456 2919 3317 2456
Size: 463 398 5417
Fragments arranged by size:
5417 463 398
KasI G'GCGC_C
Cuts at: 3708 3956 3708
Size: 248 6030
KpnI G_GTAC'C
Cuts at: 3298 3298
Size: 6278
MluI A'CGCG_T
Cuts at: 5711 5711
Size: 6278
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4949 5133 4949
Size: 184 6094
MscI TGG'CCA
Cuts at: 613 1480 2131 3110 4039 613
Size: 867 651 979 929 2852
Fragments arranged by size:
2852 979 929 867 651
MslI CAynn'nnrTG
Cuts at: 301 467 1591 3085 3241 4267 4394 301
Size: 166 1124 1494 156 1026 127 2185
Fragments arranged by size:
2185 1494 1124 1026 166 156 127
MspA1I CmG'CkG
Cuts at: 605 1408 1599 2109 2294 2492 2826 3389 3703
Size: 803 191 510 185 198 334 563 314
Cuts at: 3703 4063 5076 5321 605
Size: 360 1013 245 1562
Fragments arranged by size:
1562 1013 803 563 510 360 334 314
245 198 191 185
NaeI GCC'GGC
Cuts at: 4459 4459
Size: 6278
NarI GG'CG_CC
Cuts at: 3709 3957 3709
Size: 248 6030
NcoI C'CATG_G
Cuts at: 860 1351 2555 2772 860
Size: 491 1204 217 4366
Fragments arranged by size:
4366 1204 491 217
NdeI CA'TA_TG
Cuts at: 624 624
Size: 6278
NgoMIV G'CCGG_C
Cuts at: 4457 4457
Size: 6278
NsiI A_TGCA'T
Cuts at: 519 519
Size: 6278
NspI r_CATG'y
Cuts at: 446 640 1122 1596 2550 3331 3831 4738 446
Size: 194 482 474 954 781 500 907 1986
Fragments arranged by size:
1986 954 907 781 500 482 474 194
PasI CC'CwG_GG
Cuts at: 1093 2838 1093
Size: 1745 4533
PciI A'CATG_T
Cuts at: 4734 4734
Size: 6278
PflMI CCAn_nnn'nTGG
Cuts at: 1723 1723
Size: 6278
PfoI T'CCnGG_A
Cuts at: 929 981 1520 929
Size: 52 539 5687
Fragments arranged by size:
5687 539 52
PmeI GTTT'AAAC
Cuts at: 3277 3277
Size: 6278
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5417 5449 5417
Size: 32 6246
PpuMI rG'GwC_Cy
Cuts at: 2842 2842
Size: 6278
PsiI TTA'TAA
Cuts at: 1082 1082
Size: 6278
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1615 1647 1615
Size: 32 6246
PstI C_TGCA'G
Cuts at: 559 1104 1753 2471 3325 4010 559
Size: 545 649 718 854 685 2827
Fragments arranged by size:
2827 854 718 685 649 545
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6278
PvuII CAG'CTG
Cuts at: 1408 1599 2109 3703 4063 1408
Size: 191 510 1594 360 3623
Fragments arranged by size:
3623 1594 510 360 191
RsrII CG'GwC_CG
Cuts at: 749 4473 749
Size: 3724 2554
SacI G_AGCT'C
Cuts at: 2249 3292 2249
Size: 1043 5235
SacII CC_GC'GG
Cuts at: 2493 2493
Size: 6278
SalI G'TCGA_C
Cuts at: 3315 3315
Size: 6278
SanDI GG'GwC_CC
Cuts at: 2842 2842
Size: 6278
SapI GCTCTTCn'nnn_
Cuts at: 4301 4511 4301
Size: 210 6068
SbfI CC_TGCA'GG
Cuts at: 3325 3325
Size: 6278
ScaI AGT'ACT
Cuts at: 5795 5795
Size: 6278
SfcI C'TryA_G
Cuts at: 33 555 1100 1749 2467 3321 4006 4999 5190
Size: 522 545 649 718 854 685 993 191
Cuts at: 5190 33
Size: 1121
Fragments arranged by size:
1121 993 854 718 685 649 545 522
191
SfoI GGC'GCC
Cuts at: 3710 3958 3710
Size: 248 6030
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6278
SmaI CCC'GGG
Cuts at: 2849 3300 2849
Size: 451 5827
SmlI C'TyrA_G
Cuts at: 1955 3126 3433 4348 4840 5102 1955
Size: 1171 307 915 492 262 3131
Fragments arranged by size:
3131 1171 915 492 307 262
SnaBI TAC'GTA
Cuts at: 3270 3270
Size: 6278
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6278
SphI G_CATG'C
Cuts at: 1596 3331 3831 1596
Size: 1735 500 4043
Fragments arranged by size:
4043 1735 500
StuI AGG'CCT
Cuts at: 2833 2833
Size: 6278
StyI C'CwwG_G
Cuts at: 860 1351 1659 2555 2772 3412 3766 860
Size: 491 308 896 217 640 354 3372
Fragments arranged by size:
3372 896 640 491 354 308 217
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1779 2266 3861 3994 4453 4662 1779
Size: 487 1595 133 459 209 3395
Fragments arranged by size:
3395 1595 487 459 209 133
TatI w'GTAC_w
Cuts at: 3262 5793 3262
Size: 2531 3747
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 925 957 2890 2922 5795 5827 925
Size: 32 1933 32 2873 32 1376
Fragments arranged by size:
2873 1933 1376 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 709 3165 4075 709
Size: 2456 910 2912
Fragments arranged by size:
2912 2456 910
XbaI T'CTAG_A
Cuts at: 421 3309 421
Size: 2888 3390
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1395 1724 3773 1395
Size: 329 2049 3900
Fragments arranged by size:
3900 2049 329
XhoI C'TCGA_G
Cuts at: 3126 3126
Size: 6278
XmaI C'CCGG_G
Cuts at: 2847 3298 2847
Size: 451 5827
XmnI GAAnn'nnTTC
Cuts at: 1205 1205
Size: 6278
ZraI GAC'GTC
Cuts at: 947 947
Size: 6278
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PsiI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BmtI BsmBI BstEII
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NheI NotI NruI PacI PmlI
PshAI PspOMI PspXI SexAI SfiI SgrAI SrfI SspI
SwaI