Restriction map for
pF1KSDA1369
(Circular) (Six-base) MAPSORT of: pF1KSDA1369.seq Check: 906 from: 1 to: 5027
pF1KSDA1369 5027 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 668 1087 2065 668
Size: 419 978 3630
Fragments arranged by size:
3630 978 419
Acc65I G'GTAC_C
Cuts at: 2043 2043
Size: 5027
AclI AA'CG_TT
Cuts at: 4867 4867
Size: 5027
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 1474 2852 3284 4031 4299 772
Size: 702 1378 432 747 268 1500
Fragments arranged by size:
1500 1378 747 702 432 268
AflIII A'CryG_T
Cuts at: 831 2379 3483 4460 831
Size: 1548 1104 977 1398
Fragments arranged by size:
1548 1398 1104 977
AgeI A'CCGG_T
Cuts at: 3415 3415
Size: 5027
AlwNI CAG_nnn'CTG
Cuts at: 1152 1857 2332 3899 1152
Size: 705 475 1567 2280
Fragments arranged by size:
2280 1567 705 475
ApaI G_GGCC'C
Cuts at: 226 226
Size: 5027
ApaLI G'TGCA_C
Cuts at: 1441 3797 1441
Size: 2356 2671
ApoI r'AATT_y
Cuts at: 622 1388 1878 2031 4380 4636 622
Size: 766 490 153 2349 256 1013
Fragments arranged by size:
2349 1013 766 490 256 153
AseI AT'TA_AT
Cuts at: 20 20
Size: 5027
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5027
AvaI C'yCGr_G
Cuts at: 2047 2047
Size: 5027
BamHI G'GATC_C
Cuts at: 537 2052 537
Size: 1515 3512
BanI G'GyrC_C
Cuts at: 2043 2457 2705 2740 2043
Size: 414 248 35 4330
Fragments arranged by size:
4330 414 248 35
BanII G_rGCy'C
Cuts at: 226 977 1100 1292 1694 2041 3071 226
Size: 751 123 192 402 347 1030 2182
Fragments arranged by size:
2182 1030 751 402 347 192 123
BbeI G_GCGC'C
Cuts at: 2461 2709 2461
Size: 248 4779
BbsI GAAGACnn'nnnn_
Cuts at: 431 778 431
Size: 347 4680
BciVI GTATCCnnnnn_n'
Cuts at: 571 649 2008 2925 3686 5027 571
Size: 78 1359 917 761 1341 571
Fragments arranged by size:
1359 1341 917 761 571 78
BclI T'GATC_A
Cuts at: 1485 2547 1485
Size: 1062 3965
BglI GCCn_nnn'nGGC
Cuts at: 1161 1161
Size: 5027
BglII A'GATC_T
Cuts at: 1449 2542 4579 1449
Size: 1093 2037 1897
Fragments arranged by size:
2037 1897 1093
BlpI GC'TnA_GC
Cuts at: 971 2139 971
Size: 1168 3859
Bme1580I G_kGCm'C
Cuts at: 226 1445 2652 2745 3801 226
Size: 1219 1207 93 1056 1452
Fragments arranged by size:
1452 1219 1207 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2239 2320 2655 4799 2239
Size: 81 335 2144 2467
Fragments arranged by size:
2467 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 483 3394 483
Size: 2911 2116
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 1084 1114 364
Size: 720 30 4277
Fragments arranged by size:
4277 720 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 372 1102 1955 2203 3082 3574 3872 372
Size: 730 853 248 879 492 298 1527
Fragments arranged by size:
1527 879 853 730 492 298 248
BsaI GGTCTCn'nnnn_
Cuts at: 1830 4731 1830
Size: 2901 2126
BsaAI yAC'GTr
Cuts at: 360 2019 2380 360
Size: 1659 361 3007
Fragments arranged by size:
3007 1659 361
BsaBI GATnn'nnATC
Cuts at: 6 2541 4578 6
Size: 2535 2037 455
Fragments arranged by size:
2535 2037 455
BsaHI Gr'CG_yC
Cuts at: 2458 2706 3408 4904 2458
Size: 248 702 1496 2581
Fragments arranged by size:
2581 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2216 2737 3415 3689 3836 4247 2216
Size: 521 678 274 147 411 2996
Fragments arranged by size:
2996 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 178 1195 178
Size: 1017 4010
BseYI C'CCAG_C
Cuts at: 178 2452 3210 3787 4283 178
Size: 2274 758 577 496 922
Fragments arranged by size:
2274 922 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1249 1506 1249
Size: 257 4770
BsiEI CG_ry'CG
Cuts at: 104 2569 2615 3823 104
Size: 2465 46 1208 1308
Fragments arranged by size:
2465 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 350 896 1100 1292 1445 1694 2041 2819 3009
Size: 546 204 192 153 249 347 778 190
Cuts at: 3009 3801 350
Size: 792 1576
Fragments arranged by size:
1576 792 778 546 347 249 204 192
190 153
Bsp1286I G_dGCh'C
Cuts at: 226 350 896 977 1100 1292 1445 1694 2041
Size: 124 546 81 123 192 153 249 347
Cuts at: 2041 2652 2745 2819 3009 3071 3801 226
Size: 611 93 74 190 62 730 1452
Fragments arranged by size:
1452 730 611 546 347 249 192 190
153 124 123 93 81 74 62
BspEI T'CCGG_A
Cuts at: 4247 4247
Size: 5027
BspHI T'CATG_A
Cuts at: 4203 4203
Size: 5027
BspMI ACCTGCnnnn'nnnn_
Cuts at: 772 2077 2291 2593 2974 772
Size: 1305 214 302 381 2825
Fragments arranged by size:
2825 1305 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3319 3373 4857 5022 50
Size: 3269 54 1484 165 55
Fragments arranged by size:
3269 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 928 2939 928
Size: 2011 3016
BsrFI r'CCGG_y
Cuts at: 198 3025 3206 3415 198
Size: 2827 181 209 1810
Fragments arranged by size:
2827 1810 209 181
BsrGI T'GTAC_A
Cuts at: 1664 1664
Size: 5027
BssSI C'ACGA_G
Cuts at: 851 3298 3656 851
Size: 2447 358 2222
Fragments arranged by size:
2447 2222 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2404 2404
Size: 5027
BstBI TT'CG_AA
Cuts at: 110 3388 110
Size: 3278 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1108 1882 1108
Size: 774 4253
BstYI r'GATC_y
Cuts at: 1 537 1449 1636 2052 2519 2542 2877 3123
Size: 536 912 187 416 467 23 335 246
Cuts at: 3123 4135 4221 4233 4579 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 912 536 467 449 416 346 335
246 187 86 23 12
BstZ17I GTA'TAC
Cuts at: 669 669
Size: 5027
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1814 2977 3158 3321 1814
Size: 1163 181 163 3520
Fragments arranged by size:
3520 1163 181 163
BtsI GCAGTG_nn'
Cuts at: 2270 2270
Size: 5027
DraI TTT'AAA
Cuts at: 2026 2026
Size: 5027
DraIII CAC_nnn'GTG
Cuts at: 821 821
Size: 5027
DrdI GACnn_nn'nnGTC
Cuts at: 2733 3591 2733
Size: 858 4169
EaeI y'GGCC_r
Cuts at: 200 1370 2612 2786 3177 3204 4549 200
Size: 1170 1242 174 391 27 1345 678
Fragments arranged by size:
1345 1242 1170 678 391 174 27
EagI C'GGCC_G
Cuts at: 2612 2612
Size: 5027
EarI CTCTTCn'nnn_
Cuts at: 155 657 902 1564 3050 3260 155
Size: 502 245 662 1486 210 1922
Fragments arranged by size:
1922 1486 662 502 245 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3235 3545 3691 4837 3235
Size: 310 146 1146 3425
Fragments arranged by size:
3425 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1098 1290 1692 2039 1098
Size: 192 402 347 4086
Fragments arranged by size:
4086 402 347 192
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 772 1084 1114 1474 2852 3284 4031 4299
Size: 408 312 30 360 1378 432 747 268
Cuts at: 4299 364
Size: 1092
Fragments arranged by size:
1378 1092 747 432 408 360 312 268
30
EcoNI CCTnn'n_nnAGG
Cuts at: 724 724
Size: 5027
EcoO109I rG'GnC_Cy
Cuts at: 222 756 2166 222
Size: 534 1410 3083
Fragments arranged by size:
3083 1410 534
EcoRI G'AATT_C
Cuts at: 2031 2031
Size: 5027
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3046 3046
Size: 5027
FspI TGC'GCA
Cuts at: 2808 2808
Size: 5027
HaeII r_GCGC'y
Cuts at: 2461 2709 3731 4710 2461
Size: 248 1022 979 2778
Fragments arranged by size:
2778 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 465 497 516 548 865 897 1639 1671 2869
Size: 32 19 32 317 32 742 32 1198
Cuts at: 2869 2899 2901 2931 465
Size: 30 2 30 2561
Fragments arranged by size:
2561 1198 742 317 32 32 32 32
30 30 19 2
HincII GTy'rAC
Cuts at: 1088 1224 1893 2066 1088
Size: 136 669 173 4049
Fragments arranged by size:
4049 669 173 136
HpaI GTT'AAC
Cuts at: 1893 1893
Size: 5027
KasI G'GCGC_C
Cuts at: 2457 2705 2457
Size: 248 4779
KpnI G_GTAC'C
Cuts at: 2047 2047
Size: 5027
MfeI C'AATT_G
Cuts at: 1136 1136
Size: 5027
MluI A'CGCG_T
Cuts at: 4460 4460
Size: 5027
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 923 1610 3698 3882 923
Size: 687 2088 184 2068
Fragments arranged by size:
2088 2068 687 184
MscI TGG'CCA
Cuts at: 1372 2788 1372
Size: 1416 3611
MslI CAynn'nnrTG
Cuts at: 362 836 1273 3016 3143 362
Size: 474 437 1743 127 2246
Fragments arranged by size:
2246 1743 474 437 127
MspA1I CmG'CkG
Cuts at: 1152 1281 1854 2138 2452 2812 3825 4070 1152
Size: 129 573 284 314 360 1013 245 2109
Fragments arranged by size:
2109 1013 573 360 314 284 245 129
NaeI GCC'GGC
Cuts at: 200 3208 200
Size: 3008 2019
NarI GG'CG_CC
Cuts at: 2458 2706 2458
Size: 248 4779
NgoMIV G'CCGG_C
Cuts at: 198 3206 198
Size: 3008 2019
NspI r_CATG'y
Cuts at: 835 2080 2580 3487 835
Size: 1245 500 907 2375
Fragments arranged by size:
2375 1245 907 500
PciI A'CATG_T
Cuts at: 831 3483 831
Size: 2652 2375
PfoI T'CCnGG_A
Cuts at: 1100 1100
Size: 5027
PmeI GTTT'AAAC
Cuts at: 2026 2026
Size: 5027
PmlI CAC'GTG
Cuts at: 360 360
Size: 5027
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1715 1747 4166 4198 1715
Size: 32 2419 32 2544
Fragments arranged by size:
2544 2419 32 32
PpuMI rG'GwC_Cy
Cuts at: 756 756
Size: 5027
PshAI GACnn'nnGTC
Cuts at: 1511 1511
Size: 5027
PspOMI G'GGCC_C
Cuts at: 222 222
Size: 5027
PstI C_TGCA'G
Cuts at: 1862 2074 2759 1862
Size: 212 685 4130
Fragments arranged by size:
4130 685 212
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5027
PvuII CAG'CTG
Cuts at: 1152 1281 1854 2452 2812 1152
Size: 129 573 598 360 3367
Fragments arranged by size:
3367 598 573 360 129
RsrII CG'GwC_CG
Cuts at: 3222 3222
Size: 5027
SacI G_AGCT'C
Cuts at: 1100 1292 1694 2041 1100
Size: 192 402 347 4086
Fragments arranged by size:
4086 402 347 192
SalI G'TCGA_C
Cuts at: 1086 2064 1086
Size: 978 4049
SapI GCTCTTCn'nnn_
Cuts at: 3050 3260 3050
Size: 210 4817
SbfI CC_TGCA'GG
Cuts at: 2074 2074
Size: 5027
ScaI AGT'ACT
Cuts at: 4544 4544
Size: 5027
SfcI C'TryA_G
Cuts at: 33 1534 1858 1903 2070 2755 3748 3939 33
Size: 1501 324 45 167 685 993 191 1121
Fragments arranged by size:
1501 1121 993 685 324 191 167 45
SfoI GGC'GCC
Cuts at: 2459 2707 2459
Size: 248 4779
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5027
SmaI CCC'GGG
Cuts at: 2049 2049
Size: 5027
SmlI C'TyrA_G
Cuts at: 351 1081 1970 2182 3097 3589 3851 351
Size: 730 889 212 915 492 262 1527
Fragments arranged by size:
1527 915 889 730 492 262 212
SnaBI TAC'GTA
Cuts at: 2019 2019
Size: 5027
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5027
SphI G_CATG'C
Cuts at: 2080 2580 2080
Size: 500 4527
SspI AAT'ATT
Cuts at: 801 1467 801
Size: 666 4361
StyI C'CwwG_G
Cuts at: 590 2161 2515 590
Size: 1571 354 3102
Fragments arranged by size:
3102 1571 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1663 2610 2743 3202 3411 1663
Size: 947 133 459 209 3279
Fragments arranged by size:
3279 947 459 209 133
TatI w'GTAC_w
Cuts at: 1043 1664 4542 1043
Size: 621 2878 1528
Fragments arranged by size:
2878 1528 621
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4544 4576 4544
Size: 32 4995
Tth111I GACn'n_nGTC
Cuts at: 2824 2824
Size: 5027
XbaI T'CTAG_A
Cuts at: 2058 2058
Size: 5027
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1137 2522 1137
Size: 1385 3642
XmaI C'CCGG_G
Cuts at: 2047 2047
Size: 5027
XmnI GAAnn'nnTTC
Cuts at: 833 833
Size: 5027
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BstZ17I BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmI BsmBI BssHII BstEII Bsu36I
BtgI ClaI CspCI EcoRV FseI FspAI HindIII NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI StuI SwaI XhoI ZraI