Restriction map for
pF1KSDA1379
(Circular) (Six-base) MAPSORT of: pF1KSDA1379.seq Check: 4169 from: 1 to: 4469
pF1KSDA1379 4469 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 801 801
Size: 4469
AccI GT'mk_AC
Cuts at: 1507 1507
Size: 4469
Acc65I G'GTAC_C
Cuts at: 1485 1485
Size: 4469
AclI AA'CG_TT
Cuts at: 4309 4309
Size: 4469
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 551 2294 2726 3473 3741 551
Size: 1743 432 747 268 1279
Fragments arranged by size:
1743 1279 747 432 268
AflIII A'CryG_T
Cuts at: 1821 2925 3902 1821
Size: 1104 977 2388
Fragments arranged by size:
2388 1104 977
AgeI A'CCGG_T
Cuts at: 2857 2857
Size: 4469
AlwNI CAG_nnn'CTG
Cuts at: 182 1774 3341 182
Size: 1592 1567 1310
Fragments arranged by size:
1592 1567 1310
ApaLI G'TGCA_C
Cuts at: 3239 3239
Size: 4469
ApoI r'AATT_y
Cuts at: 1473 3822 4078 1473
Size: 2349 256 1864
Fragments arranged by size:
2349 1864 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4469
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4469
AvaI C'yCGr_G
Cuts at: 1489 1489
Size: 4469
BamHI G'GATC_C
Cuts at: 1494 1494
Size: 4469
BanI G'GyrC_C
Cuts at: 156 326 379 887 1255 1394 1485 1899 2147
Size: 170 53 508 368 139 91 414 248
Cuts at: 2147 2182 156
Size: 35 2443
Fragments arranged by size:
2443 508 414 368 248 170 139 91
53 35
BanII G_rGCy'C
Cuts at: 1337 1483 2513 1337
Size: 146 1030 3293
Fragments arranged by size:
3293 1030 146
BbeI G_GCGC'C
Cuts at: 160 330 1259 1903 2151 160
Size: 170 929 644 248 2478
Fragments arranged by size:
2478 929 644 248 170
BbsI GAAGACnn'nnnn_
Cuts at: 742 846 977 742
Size: 104 131 4234
Fragments arranged by size:
4234 131 104
BciVI GTATCCnnnnn_n'
Cuts at: 2367 3128 4469 2367
Size: 761 1341 2367
Fragments arranged by size:
2367 1341 761
BclI T'GATC_A
Cuts at: 1989 1989
Size: 4469
BglI GCCn_nnn'nGGC
Cuts at: 154 598 1123 154
Size: 444 525 3500
Fragments arranged by size:
3500 525 444
BglII A'GATC_T
Cuts at: 1984 4021 1984
Size: 2037 2432
BlpI GC'TnA_GC
Cuts at: 1336 1581 1336
Size: 245 4224
Bme1580I G_kGCm'C
Cuts at: 1302 2094 2187 3243 1302
Size: 792 93 1056 2528
Fragments arranged by size:
2528 1056 792 93
BmrI ACTGGGnnnn_n'
Cuts at: 321 783 1020 1130 1681 1762 2097 4241 321
Size: 462 237 110 551 81 335 2144 549
Fragments arranged by size:
2144 551 549 462 335 237 110 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1293 1375 2836 1293
Size: 82 1461 2926
Fragments arranged by size:
2926 1461 82
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 475 604 958 385
Size: 90 129 354 3896
Fragments arranged by size:
3896 354 129 90
Bpu10I CC'TnA_GC
Cuts at: 680 680
Size: 4469
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 1645 2524 3016 3314 844
Size: 801 879 492 298 1999
Fragments arranged by size:
1999 879 801 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 160 1237 4173 160
Size: 1077 2936 456
Fragments arranged by size:
2936 1077 456
BsaAI yAC'GTr
Cuts at: 1446 1461 1822 1446
Size: 15 361 4093
Fragments arranged by size:
4093 361 15
BsaBI GATnn'nnATC
Cuts at: 6 1983 4020 6
Size: 1977 2037 455
Fragments arranged by size:
2037 1977 455
BsaHI Gr'CG_yC
Cuts at: 157 327 482 1256 1900 2148 2850 4346 157
Size: 170 155 774 644 248 702 1496 280
Fragments arranged by size:
1496 774 702 644 280 248 170 155
BsaWI w'CCGG_w
Cuts at: 1658 2179 2857 3131 3278 3689 1658
Size: 521 678 274 147 411 2438
Fragments arranged by size:
2438 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 799 829 1277 1307 799
Size: 30 448 30 3961
Fragments arranged by size:
3961 448 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 178 1048 1390 178
Size: 870 342 3257
Fragments arranged by size:
3257 870 342
BseYI C'CCAG_C
Cuts at: 1147 1368 1422 1894 2652 3229 3725 1147
Size: 221 54 472 758 577 496 1891
Fragments arranged by size:
1891 758 577 496 472 221 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 400 751 283
Size: 117 351 4001
Fragments arranged by size:
4001 351 117
BsiEI CG_ry'CG
Cuts at: 104 2011 2057 3265 104
Size: 1907 46 1208 1308
Fragments arranged by size:
1907 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1337 1483 2261 2451 3243 1337
Size: 146 778 190 792 2563
Fragments arranged by size:
2563 792 778 190 146
BsiWI C'GTAC_G
Cuts at: 290 290
Size: 4469
BsmBI CGTCTCn'nnnn_
Cuts at: 511 1346 511
Size: 835 3634
Bsp1286I G_dGCh'C
Cuts at: 1302 1337 1483 2094 2187 2261 2451 2513 3243
Size: 35 146 611 93 74 190 62 730
Cuts at: 3243 1302
Size: 2528
Fragments arranged by size:
2528 730 611 190 146 93 74 62
35
BspEI T'CCGG_A
Cuts at: 3689 3689
Size: 4469
BspHI T'CATG_A
Cuts at: 3645 3645
Size: 4469
BspMI ACCTGCnnnn'nnnn_
Cuts at: 801 1519 1733 2035 2416 801
Size: 718 214 302 381 2854
Fragments arranged by size:
2854 718 381 302 214
BsrBI CCG'CTC
Cuts at: 50 369 2761 2815 4299 4464 50
Size: 319 2392 54 1484 165 55
Fragments arranged by size:
2392 1484 319 165 55 54
BsrDI GCAATG_nn'
Cuts at: 829 2381 829
Size: 1552 2917
BsrFI r'CCGG_y
Cuts at: 2467 2648 2857 2467
Size: 181 209 4079
Fragments arranged by size:
4079 209 181
BssHII G'CGCG_C
Cuts at: 270 270
Size: 4469
BssSI C'ACGA_G
Cuts at: 2740 3098 2740
Size: 358 4111
BstAPI GCAn_nnn'nTGC
Cuts at: 729 1846 729
Size: 1117 3352
BstBI TT'CG_AA
Cuts at: 110 2830 110
Size: 2720 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1446 1446
Size: 4469
BstYI r'GATC_y
Cuts at: 1 1494 1961 1984 2319 2565 3577 3663 3675
Size: 1493 467 23 335 246 1012 86 12
Cuts at: 3675 4021 1
Size: 346 449
Fragments arranged by size:
1493 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 1155 1398 1155
Size: 243 4226
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2419 2600 2763 2419
Size: 181 163 4125
Fragments arranged by size:
4125 181 163
BtsI GCAGTG_nn'
Cuts at: 1167 1245 1712 1167
Size: 78 467 3924
Fragments arranged by size:
3924 467 78
ClaI AT'CG_AT
Cuts at: 221 221
Size: 4469
DraI TTT'AAA
Cuts at: 1468 1468
Size: 4469
DraIII CAC_nnn'GTG
Cuts at: 1202 1202
Size: 4469
DrdI GACnn_nn'nnGTC
Cuts at: 2175 3033 2175
Size: 858 3611
EaeI y'GGCC_r
Cuts at: 228 632 1317 2054 2228 2619 2646 3991 228
Size: 404 685 737 174 391 27 1345 706
Fragments arranged by size:
1345 737 706 685 404 391 174 27
EagI C'GGCC_G
Cuts at: 2054 2054
Size: 4469
EarI CTCTTCn'nnn_
Cuts at: 2492 2702 2492
Size: 210 4259
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2677 2987 3133 4279 2677
Size: 310 146 1146 2867
Fragments arranged by size:
2867 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1335 1481 1335
Size: 146 4323
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 475 551 604 958 2294 2726 3473 3741
Size: 90 76 53 354 1336 432 747 268
Cuts at: 3741 385
Size: 1113
Fragments arranged by size:
1336 1113 747 432 354 268 90 76
53
EcoNI CCTnn'n_nnAGG
Cuts at: 457 862 457
Size: 405 4064
EcoO109I rG'GnC_Cy
Cuts at: 450 1608 450
Size: 1158 3311
EcoRI G'AATT_C
Cuts at: 1473 1473
Size: 4469
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2488 2488
Size: 4469
FspI TGC'GCA
Cuts at: 2250 2250
Size: 4469
HaeII r_GCGC'y
Cuts at: 160 330 1108 1259 1903 2151 3173 4152 160
Size: 170 778 151 644 248 1022 979 477
Fragments arranged by size:
1022 979 778 644 477 248 170 151
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 133 165 1123 1155 2311 2341 2343 2373 133
Size: 32 958 32 1156 30 2 30 2229
Fragments arranged by size:
2229 1156 958 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1508 1508
Size: 4469
KasI G'GCGC_C
Cuts at: 156 326 1255 1899 2147 156
Size: 170 929 644 248 2478
Fragments arranged by size:
2478 929 644 248 170
KpnI G_GTAC'C
Cuts at: 1489 1489
Size: 4469
MluI A'CGCG_T
Cuts at: 3902 3902
Size: 4469
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3140 3324 3140
Size: 184 4285
MscI TGG'CCA
Cuts at: 634 2230 634
Size: 1596 2873
MslI CAynn'nnrTG
Cuts at: 922 1012 2458 2585 922
Size: 90 1446 127 2806
Fragments arranged by size:
2806 1446 127 90
MspA1I CmG'CkG
Cuts at: 1157 1415 1422 1580 1894 2254 3267 3512 1157
Size: 258 7 158 314 360 1013 245 2114
Fragments arranged by size:
2114 1013 360 314 258 245 158 7
NaeI GCC'GGC
Cuts at: 2650 2650
Size: 4469
NarI GG'CG_CC
Cuts at: 157 327 1256 1900 2148 157
Size: 170 929 644 248 2478
Fragments arranged by size:
2478 929 644 248 170
NgoMIV G'CCGG_C
Cuts at: 2648 2648
Size: 4469
NspI r_CATG'y
Cuts at: 1011 1522 2022 2929 1011
Size: 511 500 907 2551
Fragments arranged by size:
2551 907 511 500
PciI A'CATG_T
Cuts at: 2925 2925
Size: 4469
PflMI CCAn_nnn'nTGG
Cuts at: 323 355 795 1019 1202 323
Size: 32 440 224 183 3590
Fragments arranged by size:
3590 440 224 183 32
PmeI GTTT'AAAC
Cuts at: 1468 1468
Size: 4469
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3608 3640 3608
Size: 32 4437
PpuMI rG'GwC_Cy
Cuts at: 450 450
Size: 4469
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 898 930 898
Size: 32 4437
PstI C_TGCA'G
Cuts at: 701 1516 2201 701
Size: 815 685 2969
Fragments arranged by size:
2969 815 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4469
PvuII CAG'CTG
Cuts at: 1422 1894 2254 1422
Size: 472 360 3637
Fragments arranged by size:
3637 472 360
RsrII CG'GwC_CG
Cuts at: 206 2664 206
Size: 2458 2011
SacI G_AGCT'C
Cuts at: 1337 1483 1337
Size: 146 4323
SacII CC_GC'GG
Cuts at: 1158 1158
Size: 4469
SalI G'TCGA_C
Cuts at: 1506 1506
Size: 4469
SapI GCTCTTCn'nnn_
Cuts at: 2492 2702 2492
Size: 210 4259
SbfI CC_TGCA'GG
Cuts at: 1516 1516
Size: 4469
ScaI AGT'ACT
Cuts at: 3986 3986
Size: 4469
SfcI C'TryA_G
Cuts at: 33 697 1512 2197 3190 3381 33
Size: 664 815 685 993 191 1121
Fragments arranged by size:
1121 993 815 685 664 191
SfoI GGC'GCC
Cuts at: 158 328 1257 1901 2149 158
Size: 170 929 644 248 2478
Fragments arranged by size:
2478 929 644 248 170
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4469
SmaI CCC'GGG
Cuts at: 1491 1491
Size: 4469
SmlI C'TyrA_G
Cuts at: 859 1624 2539 3031 3293 859
Size: 765 915 492 262 2035
Fragments arranged by size:
2035 915 765 492 262
SnaBI TAC'GTA
Cuts at: 1461 1461
Size: 4469
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4469
SphI G_CATG'C
Cuts at: 1011 1522 2022 1011
Size: 511 500 3458
Fragments arranged by size:
3458 511 500
StuI AGG'CCT
Cuts at: 148 601 148
Size: 453 4016
StyI C'CwwG_G
Cuts at: 559 1280 1603 1957 559
Size: 721 323 354 3071
Fragments arranged by size:
3071 721 354 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 493 660 2052 2185 2644 2853 493
Size: 167 1392 133 459 209 2109
Fragments arranged by size:
2109 1392 459 209 167 133
TatI w'GTAC_w
Cuts at: 791 3984 791
Size: 3193 1276
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1277 1309 3986 4018 1277
Size: 32 2677 32 1728
Fragments arranged by size:
2677 1728 32 32
Tth111I GACn'n_nGTC
Cuts at: 2266 2266
Size: 4469
XbaI T'CTAG_A
Cuts at: 1500 1500
Size: 4469
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1964 1964
Size: 4469
XmaI C'CCGG_G
Cuts at: 1489 1489
Size: 4469
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PflMI PmeI PpiI PpuMI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
BsmI BsrGI BstEII BstZ17I Bsu36I CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PshAI PsiI
PspOMI PspXI SanDI SexAI SfiI SgrAI SrfI SspI
SwaI XhoI XmnI ZraI