Restriction map for
pF1KSDA1387
(Circular) (Six-base) MAPSORT of: pF1KSDA1387.seq Check: 7491 from: 1 to: 5684
pF1KSDA1387 5684 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 2722 2722
Size: 5684
Acc65I G'GTAC_C
Cuts at: 2700 2700
Size: 5684
AclI AA'CG_TT
Cuts at: 5524 5524
Size: 5684
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 497 710 1088 1556 3509 3941 4688 4956 497
Size: 213 378 468 1953 432 747 268 1225
Fragments arranged by size:
1953 1225 747 468 432 378 268 213
AflIII A'CryG_T
Cuts at: 532 3036 4140 5117 532
Size: 2504 1104 977 1099
Fragments arranged by size:
2504 1104 1099 977
AgeI A'CCGG_T
Cuts at: 4072 4072
Size: 5684
AleI CACnn'nnGTG
Cuts at: 217 217
Size: 5684
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1412 1444 1412
Size: 32 5652
AlwNI CAG_nnn'CTG
Cuts at: 895 2193 2989 4556 895
Size: 1298 796 1567 2023
Fragments arranged by size:
2023 1567 1298 796
ApaLI G'TGCA_C
Cuts at: 4454 4454
Size: 5684
ApoI r'AATT_y
Cuts at: 413 844 981 1214 1300 1507 1515 1863 1933
Size: 431 137 233 86 207 8 348 70
Cuts at: 1933 2022 2047 2443 2688 5037 5293 413
Size: 89 25 396 245 2349 256 804
Fragments arranged by size:
2349 804 431 396 348 256 245 233
207 137 89 86 70 25 8
AseI AT'TA_AT
Cuts at: 20 331 1373 20
Size: 311 1042 4331
Fragments arranged by size:
4331 1042 311
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5684
AvaI C'yCGr_G
Cuts at: 2704 2704
Size: 5684
BamHI G'GATC_C
Cuts at: 2530 2709 2530
Size: 179 5505
BanI G'GyrC_C
Cuts at: 193 2313 2700 3114 3362 3397 193
Size: 2120 387 414 248 35 2480
Fragments arranged by size:
2480 2120 414 387 248 35
BanII G_rGCy'C
Cuts at: 230 2698 3728 230
Size: 2468 1030 2186
Fragments arranged by size:
2468 2186 1030
BbeI G_GCGC'C
Cuts at: 3118 3366 3118
Size: 248 5436
BbsI GAAGACnn'nnnn_
Cuts at: 176 1583 2158 2405 176
Size: 1407 575 247 3455
Fragments arranged by size:
3455 1407 575 247
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2099 2133 2099
Size: 34 5650
BciVI GTATCCnnnnn_n'
Cuts at: 126 318 3582 4343 5684 126
Size: 192 3264 761 1341 126
Fragments arranged by size:
3264 1341 761 192 126
BclI T'GATC_A
Cuts at: 3204 3204
Size: 5684
BfrBI ATG'CAT
Cuts at: 1186 1539 1186
Size: 353 5331
BglII A'GATC_T
Cuts at: 401 3199 5236 401
Size: 2798 2037 849
Fragments arranged by size:
2798 2037 849
BlpI GC'TnA_GC
Cuts at: 824 1343 2796 824
Size: 519 1453 3712
Fragments arranged by size:
3712 1453 519
Bme1580I G_kGCm'C
Cuts at: 203 1896 3309 3402 4458 203
Size: 1693 1413 93 1056 1429
Fragments arranged by size:
1693 1429 1413 1056 93
BmgBI CAC'GTC
Cuts at: 204 204
Size: 5684
BmrI ACTGGGnnnn_n'
Cuts at: 2896 2977 3312 5456 2896
Size: 81 335 2144 3124
Fragments arranged by size:
3124 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4051 4051
Size: 5684
Bpu10I CC'TnA_GC
Cuts at: 1412 2330 1412
Size: 918 4766
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 1670 2860 3739 4231 4529 214
Size: 1456 1190 879 492 298 1369
Fragments arranged by size:
1456 1369 1190 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5388 5388
Size: 5684
BsaAI yAC'GTr
Cuts at: 219 533 2676 3037 219
Size: 314 2143 361 2866
Fragments arranged by size:
2866 2143 361 314
BsaBI GATnn'nnATC
Cuts at: 6 1406 1736 3198 5235 6
Size: 1400 330 1462 2037 455
Fragments arranged by size:
2037 1462 1400 455 330
BsaHI Gr'CG_yC
Cuts at: 3115 3363 4065 5561 3115
Size: 248 702 1496 3238
Fragments arranged by size:
3238 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2873 3394 4072 4346 4493 4904 2873
Size: 521 678 274 147 411 3653
Fragments arranged by size:
3653 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 193 223 1412 1442 193
Size: 30 1189 30 4435
Fragments arranged by size:
4435 1189 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 198 238 576 198
Size: 40 338 5306
Fragments arranged by size:
5306 338 40
BseYI C'CCAG_C
Cuts at: 3109 3867 4444 4940 3109
Size: 758 577 496 3853
Fragments arranged by size:
3853 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 189 3226 3272 4480 104
Size: 85 3037 46 1208 1308
Fragments arranged by size:
3037 1308 1208 85 46
BsiHKAI G_wGCw'C
Cuts at: 230 584 2698 3476 3666 4458 230
Size: 354 2114 778 190 792 1456
Fragments arranged by size:
2114 1456 792 778 354 190
BsmBI CGTCTCn'nnnn_
Cuts at: 210 210
Size: 5684
Bsp1286I G_dGCh'C
Cuts at: 203 230 584 1896 2698 3309 3402 3476 3666
Size: 27 354 1312 802 611 93 74 190
Cuts at: 3666 3728 4458 203
Size: 62 730 1429
Fragments arranged by size:
1429 1312 802 730 611 354 190 93
74 62 27
BspEI T'CCGG_A
Cuts at: 4904 4904
Size: 5684
BspHI T'CATG_A
Cuts at: 4860 4860
Size: 5684
BspMI ACCTGCnnnn'nnnn_
Cuts at: 533 2734 2948 3250 3631 533
Size: 2201 214 302 381 2586
Fragments arranged by size:
2586 2201 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3976 4030 5514 5679 50
Size: 3926 54 1484 165 55
Fragments arranged by size:
3926 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 184 3596 184
Size: 3412 2272
BsrFI r'CCGG_y
Cuts at: 173 3682 3863 4072 173
Size: 3509 181 209 1785
Fragments arranged by size:
3509 1785 209 181
BssSI C'ACGA_G
Cuts at: 3955 4313 3955
Size: 358 5326
BstAPI GCAn_nnn'nTGC
Cuts at: 3061 3061
Size: 5684
BstBI TT'CG_AA
Cuts at: 110 4045 110
Size: 3935 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 219 219
Size: 5684
BstYI r'GATC_y
Cuts at: 1 401 2530 2709 3176 3199 3534 3780 4792
Size: 400 2129 179 467 23 335 246 1012
Cuts at: 4792 4878 4890 5236 1
Size: 86 12 346 449
Fragments arranged by size:
2129 1012 467 449 400 346 335 246
179 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3634 3815 3978 3634
Size: 181 163 5340
Fragments arranged by size:
5340 181 163
BtsI GCAGTG_nn'
Cuts at: 2927 2927
Size: 5684
ClaI AT'CG_AT
Cuts at: 2119 2119
Size: 5684
DraI TTT'AAA
Cuts at: 1604 2683 1604
Size: 1079 4605
DrdI GACnn_nn'nnGTC
Cuts at: 3390 4248 3390
Size: 858 4826
EaeI y'GGCC_r
Cuts at: 3269 3443 3834 3861 5206 3269
Size: 174 391 27 1345 3747
Fragments arranged by size:
3747 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3269 3269
Size: 5684
EarI CTCTTCn'nnn_
Cuts at: 548 971 1677 1839 2249 2285 2541 2612 3707
Size: 423 706 162 410 36 256 71 1095
Cuts at: 3707 3917 548
Size: 210 2315
Fragments arranged by size:
2315 1095 706 423 410 256 210 162
71 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1926 3892 4202 4348 5494 1926
Size: 1966 310 146 1146 2116
Fragments arranged by size:
2116 1966 1146 310 146
EcoICRI GAG'CTC
Cuts at: 228 2696 228
Size: 2468 3216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 497 710 1088 1556 3509 3941 4688 4956 497
Size: 213 378 468 1953 432 747 268 1225
Fragments arranged by size:
1953 1225 747 468 432 378 268 213
EcoNI CCTnn'n_nnAGG
Cuts at: 739 1415 739
Size: 676 5008
EcoO109I rG'GnC_Cy
Cuts at: 459 2823 459
Size: 2364 3320
EcoRI G'AATT_C
Cuts at: 844 1214 1863 2022 2688 844
Size: 370 649 159 666 3840
Fragments arranged by size:
3840 666 649 370 159
EcoRV GAT'ATC
Cuts at: 2067 2067
Size: 5684
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3703 3703
Size: 5684
FspI TGC'GCA
Cuts at: 3465 3465
Size: 5684
HaeII r_GCGC'y
Cuts at: 1427 3118 3366 4388 5367 1427
Size: 1691 248 1022 979 1744
Fragments arranged by size:
1744 1691 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 137 169 260 292 1838 1870 2056 2088 3526
Size: 32 91 32 1546 32 186 32 1438
Cuts at: 3526 3556 3558 3588 137
Size: 30 2 30 2233
Fragments arranged by size:
2233 1546 1438 186 91 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2723 2723
Size: 5684
HindIII A'AGCT_T
Cuts at: 665 665
Size: 5684
KasI G'GCGC_C
Cuts at: 3114 3362 3114
Size: 248 5436
KpnI G_GTAC'C
Cuts at: 2704 2704
Size: 5684
MluI A'CGCG_T
Cuts at: 5117 5117
Size: 5684
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 110 287 1603 1680 4355 4539 110
Size: 177 1316 77 2675 184 1255
Fragments arranged by size:
2675 1316 1255 184 177 77
MscI TGG'CCA
Cuts at: 3445 3445
Size: 5684
MslI CAynn'nnrTG
Cuts at: 217 707 3673 3800 217
Size: 490 2966 127 2101
Fragments arranged by size:
2966 2101 490 127
MspA1I CmG'CkG
Cuts at: 657 1270 2029 2795 3109 3469 4482 4727 657
Size: 613 759 766 314 360 1013 245 1614
Fragments arranged by size:
1614 1013 766 759 613 360 314 245
NaeI GCC'GGC
Cuts at: 3865 3865
Size: 5684
NarI GG'CG_CC
Cuts at: 3115 3363 3115
Size: 248 5436
NgoMIV G'CCGG_C
Cuts at: 3863 3863
Size: 5684
NsiI A_TGCA'T
Cuts at: 1188 1541 1188
Size: 353 5331
NspI r_CATG'y
Cuts at: 1041 1476 1543 2737 3237 4144 1041
Size: 435 67 1194 500 907 2581
Fragments arranged by size:
2581 1194 907 500 435 67
PciI A'CATG_T
Cuts at: 4140 4140
Size: 5684
PmeI GTTT'AAAC
Cuts at: 2683 2683
Size: 5684
PmlI CAC'GTG
Cuts at: 533 533
Size: 5684
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1412 1444 4823 4855 1412
Size: 32 3379 32 2241
Fragments arranged by size:
3379 2241 32 32
PpuMI rG'GwC_Cy
Cuts at: 459 459
Size: 5684
PsiI TTA'TAA
Cuts at: 1940 1981 2053 2105 1940
Size: 41 72 52 5519
Fragments arranged by size:
5519 72 52 41
PstI C_TGCA'G
Cuts at: 2731 3416 2731
Size: 685 4999
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5684
PvuII CAG'CTG
Cuts at: 657 1270 2029 3109 3469 657
Size: 613 759 1080 360 2872
Fragments arranged by size:
2872 1080 759 613 360
RsrII CG'GwC_CG
Cuts at: 3879 3879
Size: 5684
SacI G_AGCT'C
Cuts at: 230 2698 230
Size: 2468 3216
SalI G'TCGA_C
Cuts at: 2721 2721
Size: 5684
SapI GCTCTTCn'nnn_
Cuts at: 3707 3917 3707
Size: 210 5474
SbfI CC_TGCA'GG
Cuts at: 2731 2731
Size: 5684
ScaI AGT'ACT
Cuts at: 5201 5201
Size: 5684
SfcI C'TryA_G
Cuts at: 33 1097 1274 2495 2727 3412 4405 4596 33
Size: 1064 177 1221 232 685 993 191 1121
Fragments arranged by size:
1221 1121 1064 993 685 232 191 177
SfoI GGC'GCC
Cuts at: 3116 3364 3116
Size: 248 5436
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5684
SmaI CCC'GGG
Cuts at: 2706 2706
Size: 5684
SmlI C'TyrA_G
Cuts at: 229 1685 2839 3754 4246 4508 229
Size: 1456 1154 915 492 262 1405
Fragments arranged by size:
1456 1405 1154 915 492 262
SnaBI TAC'GTA
Cuts at: 2676 2676
Size: 5684
SpeI A'CTAG_T
Cuts at: 67 508 67
Size: 441 5243
SphI G_CATG'C
Cuts at: 2737 3237 2737
Size: 500 5184
StyI C'CwwG_G
Cuts at: 620 1955 2249 2576 2818 3172 620
Size: 1335 294 327 242 354 3132
Fragments arranged by size:
3132 1335 354 327 294 242
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 203 3267 3400 3859 4068 203
Size: 3064 133 459 209 1819
Fragments arranged by size:
3064 1819 459 209 133
TatI w'GTAC_w
Cuts at: 935 5199 935
Size: 4264 1420
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 193 225 1710 1742 5201 5233 193
Size: 32 1485 32 3459 32 644
Fragments arranged by size:
3459 1485 644 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2660 3481 2660
Size: 821 4863
XbaI T'CTAG_A
Cuts at: 2715 2715
Size: 5684
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2583 3179 2583
Size: 596 5088
XmaI C'CCGG_G
Cuts at: 2704 2704
Size: 5684
XmnI GAAnn'nnTTC
Cuts at: 2129 2411 2129
Size: 282 5402
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NsiI NspI PciI
PmeI PmlI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BbvCI BglI BmtI BpmI BsgI BsiWI
BsmI BsrGI BssHII BstEII BstZ17I Bsu36I BtgI CspCI
DraIII FseI FspAI HpaI MfeI NcoI NdeI NheI
NotI NruI PacI PasI PflMI PfoI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI
SspI StuI SwaI XhoI ZraI