Restriction map for
pF1KSDA1388
(Circular) (Six-base) MAPSORT of: pF1KSDA1388.seq Check: 2965 from: 1 to: 4883
pF1KSDA1388 4883 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1289 1289
Size: 4883
AccI GT'mk_AC
Cuts at: 1921 1921
Size: 4883
Acc65I G'GTAC_C
Cuts at: 1899 1899
Size: 4883
AclI AA'CG_TT
Cuts at: 4723 4723
Size: 4883
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 825 2708 3140 3887 4155 825
Size: 1883 432 747 268 1553
Fragments arranged by size:
1883 1553 747 432 268
AfeI AGC'GCT
Cuts at: 1774 1774
Size: 4883
AflIII A'CryG_T
Cuts at: 953 995 1616 2235 3339 4316 953
Size: 42 621 619 1104 977 1520
Fragments arranged by size:
1520 1104 977 621 619 42
AgeI A'CCGG_T
Cuts at: 799 3271 799
Size: 2472 2411
AleI CACnn'nnGTG
Cuts at: 800 1584 800
Size: 784 4099
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 301 1835 301
Size: 1534 3349
AlwNI CAG_nnn'CTG
Cuts at: 414 2188 3755 414
Size: 1774 1567 1542
Fragments arranged by size:
1774 1567 1542
ApaLI G'TGCA_C
Cuts at: 441 819 862 1044 1300 1729 3653 441
Size: 378 43 182 256 429 1924 1671
Fragments arranged by size:
1924 1671 429 378 256 182 43
ApoI r'AATT_y
Cuts at: 1887 4236 4492 1887
Size: 2349 256 2278
Fragments arranged by size:
2349 2278 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4883
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4883
AvaI C'yCGr_G
Cuts at: 966 1309 1903 966
Size: 343 594 3946
Fragments arranged by size:
3946 594 343
BamHI G'GATC_C
Cuts at: 791 1908 791
Size: 1117 3766
BanI G'GyrC_C
Cuts at: 1634 1843 1899 2313 2561 2596 1634
Size: 209 56 414 248 35 3921
Fragments arranged by size:
3921 414 248 209 56 35
BanII G_rGCy'C
Cuts at: 776 1235 1897 2927 776
Size: 459 662 1030 2732
Fragments arranged by size:
2732 1030 662 459
BbeI G_GCGC'C
Cuts at: 1847 2317 2565 1847
Size: 470 248 4165
Fragments arranged by size:
4165 470 248
BbsI GAAGACnn'nnnn_
Cuts at: 925 925
Size: 4883
BbvCI CC'TCA_GC
Cuts at: 188 635 188
Size: 447 4436
BciVI GTATCCnnnnn_n'
Cuts at: 2781 3542 4883 2781
Size: 761 1341 2781
Fragments arranged by size:
2781 1341 761
BclI T'GATC_A
Cuts at: 2403 2403
Size: 4883
BglI GCCn_nnn'nGGC
Cuts at: 1175 1343 1388 1831 1175
Size: 168 45 443 4227
Fragments arranged by size:
4227 443 168 45
BglII A'GATC_T
Cuts at: 458 2398 4435 458
Size: 1940 2037 906
Fragments arranged by size:
2037 1940 906
BlpI GC'TnA_GC
Cuts at: 506 1995 506
Size: 1489 3394
Bme1580I G_kGCm'C
Cuts at: 244 445 823 866 1048 1171 1304 1330 1417
Size: 201 378 43 182 123 133 26 87
Cuts at: 1417 1591 1675 1733 2508 2601 3657 244
Size: 174 84 58 775 93 1056 1470
Fragments arranged by size:
1470 1056 775 378 201 182 174 133
123 93 87 84 58 43 26
BmgBI CAC'GTC
Cuts at: 1785 1785
Size: 4883
BmrI ACTGGGnnnn_n'
Cuts at: 2095 2176 2511 4655 2095
Size: 81 335 2144 2323
Fragments arranged by size:
2323 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3250 3250
Size: 4883
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 505
Size: 4883
Bpu10I CC'TnA_GC
Cuts at: 188 635 188
Size: 447 4436
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 1567 2059 2938 3430 3728 439
Size: 1128 492 879 492 298 1594
Fragments arranged by size:
1594 1128 879 492 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4587 4587
Size: 4883
BsaAI yAC'GTr
Cuts at: 1299 1875 2236 1299
Size: 576 361 3946
Fragments arranged by size:
3946 576 361
BsaBI GATnn'nnATC
Cuts at: 6 2397 4434 6
Size: 2391 2037 455
Fragments arranged by size:
2391 2037 455
BsaHI Gr'CG_yC
Cuts at: 1844 2314 2562 3264 4760 1844
Size: 470 248 702 1496 1967
Fragments arranged by size:
1967 1496 702 470 248
BsaWI w'CCGG_w
Cuts at: 799 2072 2593 3271 3545 3692 4103 799
Size: 1273 521 678 274 147 411 1579
Fragments arranged by size:
1579 1273 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 219 249 478 508 565 595 219
Size: 30 229 30 57 30 4507
Fragments arranged by size:
4507 229 57 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1243 1521 1243
Size: 278 4605
BseYI C'CCAG_C
Cuts at: 1273 2308 3066 3643 4139 1273
Size: 1035 758 577 496 2017
Fragments arranged by size:
2017 1035 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 1488 1567 520
Size: 968 79 3836
Fragments arranged by size:
3836 968 79
BsiEI CG_ry'CG
Cuts at: 104 1397 2425 2471 3679 104
Size: 1293 1028 46 1208 1308
Fragments arranged by size:
1308 1293 1208 1028 46
BsiHKAI G_wGCw'C
Cuts at: 227 407 445 491 776 823 866 977 1048
Size: 180 38 46 285 47 43 111 71
Cuts at: 1048 1304 1733 1805 1897 2675 2865 3657 227
Size: 256 429 72 92 778 190 792 1453
Fragments arranged by size:
1453 792 778 429 285 256 190 180
111 92 72 71 47 46 43 38
Bsp1286I G_dGCh'C
Cuts at: 227 244 407 445 491 776 823 866 977
Size: 17 163 38 46 285 47 43 111
Cuts at: 977 1048 1171 1235 1304 1330 1417 1591 1675
Size: 71 123 64 69 26 87 174 84
Cuts at: 1675 1733 1805 1897 2508 2601 2675 2865 2927
Size: 58 72 92 611 93 74 190 62
Cuts at: 2927 3657 227
Size: 730 1453
Fragments arranged by size:
1453 730 611 285 190 174 163 123
111 93 92 87 84 74 72 71
69 64 62 58 47 46 43 38
26 17
BspEI T'CCGG_A
Cuts at: 4103 4103
Size: 4883
BspHI T'CATG_A
Cuts at: 4059 4059
Size: 4883
BspMI ACCTGCnnnn'nnnn_
Cuts at: 488 1121 1289 1634 1933 2147 2449 2830 488
Size: 633 168 345 299 214 302 381 2541
Fragments arranged by size:
2541 633 381 345 302 299 214 168
BsrBI CCG'CTC
Cuts at: 50 431 789 891 935 1062 1146 1401 3175
Size: 381 358 102 44 127 84 255 1774
Cuts at: 3175 3229 4713 4878 50
Size: 54 1484 165 55
Fragments arranged by size:
1774 1484 381 358 255 165 127 102
84 55 54 44
BsrDI GCAATG_nn'
Cuts at: 2795 2795
Size: 4883
BsrFI r'CCGG_y
Cuts at: 799 1208 1637 2881 3062 3271 799
Size: 409 429 1244 181 209 2411
Fragments arranged by size:
2411 1244 429 409 209 181
BssHII G'CGCG_C
Cuts at: 1440 1808 1440
Size: 368 4515
BssSI C'ACGA_G
Cuts at: 3154 3512 3154
Size: 358 4525
BstAPI GCAn_nnn'nTGC
Cuts at: 231 861 998 1511 2260 231
Size: 630 137 513 749 2854
Fragments arranged by size:
2854 749 630 513 137
BstBI TT'CG_AA
Cuts at: 110 3244 110
Size: 3134 1749
BstYI r'GATC_y
Cuts at: 1 458 791 1908 2375 2398 2733 2979 3991
Size: 457 333 1117 467 23 335 246 1012
Cuts at: 3991 4077 4089 4435 1
Size: 86 12 346 449
Fragments arranged by size:
1117 1012 467 457 449 346 335 333
246 86 23 12
BtgI C'CryG_G
Cuts at: 1172 1829 1172
Size: 657 4226
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2833 3014 3177 2833
Size: 181 163 4539
Fragments arranged by size:
4539 181 163
BtsI GCAGTG_nn'
Cuts at: 2126 2126
Size: 4883
DraI TTT'AAA
Cuts at: 1882 1882
Size: 4883
DraIII CAC_nnn'GTG
Cuts at: 449 827 861 945 449
Size: 378 34 84 4387
Fragments arranged by size:
4387 378 84 34
DrdI GACnn_nn'nnGTC
Cuts at: 2589 3447 2589
Size: 858 4025
EaeI y'GGCC_r
Cuts at: 1394 2468 2642 3033 3060 4405 1394
Size: 1074 174 391 27 1345 1872
Fragments arranged by size:
1872 1345 1074 391 174 27
EagI C'GGCC_G
Cuts at: 1394 2468 1394
Size: 1074 3809
EarI CTCTTCn'nnn_
Cuts at: 1324 1539 2906 3116 1324
Size: 215 1367 210 3091
Fragments arranged by size:
3091 1367 215 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 262 3091 3401 3547 4693 262
Size: 2829 310 146 1146 452
Fragments arranged by size:
2829 1146 452 310 146
EcoICRI GAG'CTC
Cuts at: 774 1895 774
Size: 1121 3762
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 825 2708 3140 3887 4155 505
Size: 320 1883 432 747 268 1233
Fragments arranged by size:
1883 1233 747 432 320 268
EcoO109I rG'GnC_Cy
Cuts at: 349 2022 349
Size: 1673 3210
EcoRI G'AATT_C
Cuts at: 1887 1887
Size: 4883
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 561 2902 561
Size: 2341 2542
FspI TGC'GCA
Cuts at: 906 2664 906
Size: 1758 3125
HaeII r_GCGC'y
Cuts at: 1142 1776 1847 2317 2565 3587 4566 1142
Size: 634 71 470 248 1022 979 1459
Fragments arranged by size:
1459 1022 979 634 470 248 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2725 2755 2757 2787 2725
Size: 30 2 30 4821
Fragments arranged by size:
4821 30 30 2
HincII GTy'rAC
Cuts at: 1922 1922
Size: 4883
HindIII A'AGCT_T
Cuts at: 837 837
Size: 4883
KasI G'GCGC_C
Cuts at: 1843 2313 2561 1843
Size: 470 248 4165
Fragments arranged by size:
4165 470 248
KpnI G_GTAC'C
Cuts at: 1903 1903
Size: 4883
MluI A'CGCG_T
Cuts at: 1616 4316 1616
Size: 2700 2183
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 110 749 1004 3554 3738 110
Size: 639 255 2550 184 1255
Fragments arranged by size:
2550 1255 639 255 184
MscI TGG'CCA
Cuts at: 2644 2644
Size: 4883
MslI CAynn'nnrTG
Cuts at: 438 800 1584 2872 2999 438
Size: 362 784 1288 127 2322
Fragments arranged by size:
2322 1288 784 362 127
MspA1I CmG'CkG
Cuts at: 634 643 734 989 1073 1467 1556 1632 1701
Size: 9 91 255 84 394 89 76 69
Cuts at: 1701 1831 1837 1994 2308 2668 3681 3926 634
Size: 130 6 157 314 360 1013 245 1591
Fragments arranged by size:
1591 1013 394 360 314 255 245 157
130 91 89 84 76 69 9 6
NaeI GCC'GGC
Cuts at: 3064 3064
Size: 4883
NarI GG'CG_CC
Cuts at: 1844 2314 2562 1844
Size: 470 248 4165
Fragments arranged by size:
4165 470 248
NcoI C'CATG_G
Cuts at: 1172 1172
Size: 4883
NgoMIV G'CCGG_C
Cuts at: 3062 3062
Size: 4883
NspI r_CATG'y
Cuts at: 957 1936 2436 3343 957
Size: 979 500 907 2497
Fragments arranged by size:
2497 979 907 500
PasI CC'CwG_GG
Cuts at: 258 258
Size: 4883
PciI A'CATG_T
Cuts at: 953 3339 953
Size: 2386 2497
PflMI CCAn_nnn'nTGG
Cuts at: 414 414
Size: 4883
PmeI GTTT'AAAC
Cuts at: 1882 1882
Size: 4883
PmlI CAC'GTG
Cuts at: 1299 1299
Size: 4883
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1732 1764 4022 4054 1732
Size: 32 2258 32 2561
Fragments arranged by size:
2561 2258 32 32
PstI C_TGCA'G
Cuts at: 290 308 332 1037 1457 1631 1930 2615 290
Size: 18 24 705 420 174 299 685 2558
Fragments arranged by size:
2558 705 685 420 299 174 24 18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4883
PvuII CAG'CTG
Cuts at: 634 734 1467 2308 2668 634
Size: 100 733 841 360 2849
Fragments arranged by size:
2849 841 733 360 100
RsrII CG'GwC_CG
Cuts at: 3078 3078
Size: 4883
SacI G_AGCT'C
Cuts at: 776 1897 776
Size: 1121 3762
SacII CC_GC'GG
Cuts at: 1832 1832
Size: 4883
SalI G'TCGA_C
Cuts at: 1920 1920
Size: 4883
SapI GCTCTTCn'nnn_
Cuts at: 2906 3116 2906
Size: 210 4673
SbfI CC_TGCA'GG
Cuts at: 332 1930 332
Size: 1598 3285
ScaI AGT'ACT
Cuts at: 4400 4400
Size: 4883
SexAI A'CCwGG_T
Cuts at: 857 941 1850 857
Size: 84 909 3890
Fragments arranged by size:
3890 909 84
SfcI C'TryA_G
Cuts at: 33 286 304 328 729 1033 1453 1627 1926
Size: 253 18 24 401 304 420 174 299
Cuts at: 1926 2611 3604 3795 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 420 401 304 299 253
191 174 24 18
SfoI GGC'GCC
Cuts at: 1845 2315 2563 1845
Size: 470 248 4165
Fragments arranged by size:
4165 470 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4883
SgrAI Cr'CCGG_yG
Cuts at: 799 1208 1637 799
Size: 409 429 4045
Fragments arranged by size:
4045 429 409
SmaI CCC'GGG
Cuts at: 1905 1905
Size: 4883
SmlI C'TyrA_G
Cuts at: 454 1309 1582 2038 2953 3445 3707 454
Size: 855 273 456 915 492 262 1630
Fragments arranged by size:
1630 915 855 492 456 273 262
SnaBI TAC'GTA
Cuts at: 1875 1875
Size: 4883
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4883
SphI G_CATG'C
Cuts at: 1936 2436 1936
Size: 500 4383
StuI AGG'CCT
Cuts at: 1093 1432 1690 1093
Size: 339 258 4286
Fragments arranged by size:
4286 339 258
StyI C'CwwG_G
Cuts at: 1172 2017 2371 1172
Size: 845 354 3684
Fragments arranged by size:
3684 845 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1240 2466 2599 3058 3267 1240
Size: 1226 133 459 209 2856
Fragments arranged by size:
2856 1226 459 209 133
TatI w'GTAC_w
Cuts at: 4398 4398
Size: 4883
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 219 251 478 510 565 597 4400 4432 219
Size: 32 227 32 55 32 3803 32 670
Fragments arranged by size:
3803 670 227 55 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2680 2680
Size: 4883
XbaI T'CTAG_A
Cuts at: 1914 1914
Size: 4883
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 480 2378 480
Size: 1898 2985
XhoI C'TCGA_G
Cuts at: 1309 1309
Size: 4883
XmaI C'CCGG_G
Cuts at: 1903 1903
Size: 4883
XmnI GAAnn'nnTTC
Cuts at: 517 922 1093 1606 517
Size: 405 171 513 3794
Fragments arranged by size:
3794 513 405 171
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PmeI PmlI PpiI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI ApaI AscI AvrII BaeI
BcgI BfrBI BmtI BsiWI BsmI BsmBI BsrGI BstEII
BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PfoI PpuMI PshAI PsiI PspOMI PspXI PsrI
SanDI SfiI SrfI SspI SwaI ZraI