Restriction map for
pF1KSDA1391
(Circular) (Six-base) MAPSORT of: pF1KSDA1391.seq Check: 2687 from: 1 to: 6710
pF1KSDA1391 6710 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 3748 3748
Size: 6710
Acc65I G'GTAC_C
Cuts at: 3726 3726
Size: 6710
AclI AA'CG_TT
Cuts at: 6550 6550
Size: 6710
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 649 1072 1302 1785 2374 3440 4535 4967 5714
Size: 423 230 483 589 1066 1095 432 747
Cuts at: 5714 5982 649
Size: 268 1377
Fragments arranged by size:
1377 1095 1066 747 589 483 432 423
268 230
AfeI AGC'GCT
Cuts at: 264 264
Size: 6710
AflII C'TTAA_G
Cuts at: 3408 3523 3408
Size: 115 6595
AflIII A'CryG_T
Cuts at: 806 2389 4062 5166 6143 806
Size: 1583 1673 1104 977 1373
Fragments arranged by size:
1673 1583 1373 1104 977
AgeI A'CCGG_T
Cuts at: 5098 5098
Size: 6710
AlwNI CAG_nnn'CTG
Cuts at: 1083 2419 4015 5582 1083
Size: 1336 1596 1567 2211
Fragments arranged by size:
2211 1596 1567 1336
ApaI G_GGCC'C
Cuts at: 2154 2154
Size: 6710
ApaLI G'TGCA_C
Cuts at: 2166 5480 2166
Size: 3314 3396
ApoI r'AATT_y
Cuts at: 777 1217 1368 1717 2711 3714 6063 6319 777
Size: 440 151 349 994 1003 2349 256 1168
Fragments arranged by size:
2349 1168 1003 994 440 349 256 151
AseI AT'TA_AT
Cuts at: 20 2822 20
Size: 2802 3908
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6710
AvaI C'yCGr_G
Cuts at: 1025 2008 3730 1025
Size: 983 1722 4005
Fragments arranged by size:
4005 1722 983
BamHI G'GATC_C
Cuts at: 3735 3735
Size: 6710
BanI G'GyrC_C
Cuts at: 3726 4140 4388 4423 3726
Size: 414 248 35 6013
Fragments arranged by size:
6013 414 248 35
BanII G_rGCy'C
Cuts at: 1004 2154 2243 2669 3574 3724 4754 1004
Size: 1150 89 426 905 150 1030 2960
Fragments arranged by size:
2960 1150 1030 905 426 150 89
BbeI G_GCGC'C
Cuts at: 4144 4392 4144
Size: 248 6462
BbsI GAAGACnn'nnnn_
Cuts at: 2351 2684 3089 2351
Size: 333 405 5972
Fragments arranged by size:
5972 405 333
BbvCI CC'TCA_GC
Cuts at: 3603 3603
Size: 6710
BciVI GTATCCnnnnn_n'
Cuts at: 2596 4608 5369 6710 2596
Size: 2012 761 1341 2596
Fragments arranged by size:
2596 2012 1341 761
BclI T'GATC_A
Cuts at: 1479 4230 1479
Size: 2751 3959
BfrBI ATG'CAT
Cuts at: 3207 3207
Size: 6710
BglII A'GATC_T
Cuts at: 1120 1470 4225 6262 1120
Size: 350 2755 2037 1568
Fragments arranged by size:
2755 2037 1568 350
BlpI GC'TnA_GC
Cuts at: 3822 3822
Size: 6710
Bme1580I G_kGCm'C
Cuts at: 2154 2170 4335 4428 5484 2154
Size: 16 2165 93 1056 3380
Fragments arranged by size:
3380 2165 1056 93 16
BmrI ACTGGGnnnn_n'
Cuts at: 1143 1494 3922 4003 4338 6482 1143
Size: 351 2428 81 335 2144 1371
Fragments arranged by size:
2428 2144 1371 351 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 281 1857 3057 5077 281
Size: 1576 1200 2020 1914
Fragments arranged by size:
2020 1914 1576 1200
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 617 979 1394 1680 2356 403
Size: 214 362 415 286 676 4757
Fragments arranged by size:
4757 676 415 362 286 214
Bpu10I CC'TnA_GC
Cuts at: 2032 3603 2032
Size: 1571 5139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2617 3886 4765 5257 5555 2617
Size: 1269 879 492 298 3772
Fragments arranged by size:
3772 1269 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1494 2758 6414 1494
Size: 1264 3656 1790
Fragments arranged by size:
3656 1790 1264
BsaAI yAC'GTr
Cuts at: 2493 3702 4063 2493
Size: 1209 361 5140
Fragments arranged by size:
5140 1209 361
BsaBI GATnn'nnATC
Cuts at: 6 4224 6261 6
Size: 4218 2037 455
Fragments arranged by size:
4218 2037 455
BsaHI Gr'CG_yC
Cuts at: 4141 4389 5091 6587 4141
Size: 248 702 1496 4264
Fragments arranged by size:
4264 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3121 3899 4420 5098 5372 5519 5930 3121
Size: 778 521 678 274 147 411 3901
Fragments arranged by size:
3901 778 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1367 1397 3027 3057 1367
Size: 30 1630 30 5020
Fragments arranged by size:
5020 1630 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 168 270 273 1863 2782 168
Size: 102 3 1590 919 4096
Fragments arranged by size:
4096 1590 919 102 3
BseYI C'CCAG_C
Cuts at: 143 1057 1076 2173 2242 3162 3226 4135 4893
Size: 914 19 1097 69 920 64 909 758
Cuts at: 4893 5470 5966 143
Size: 577 496 887
Fragments arranged by size:
1097 920 914 909 887 758 577 496
69 64 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 204 932 3457 204
Size: 728 2525 3457
Fragments arranged by size:
3457 2525 728
BsiEI CG_ry'CG
Cuts at: 104 4252 4298 5506 104
Size: 4148 46 1208 1308
Fragments arranged by size:
4148 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 370 2170 3574 3724 4502 4692 5484 370
Size: 1800 1404 150 778 190 792 1596
Fragments arranged by size:
1800 1596 1404 792 778 190 150
BsmI GAATG_Cn'
Cuts at: 710 1842 3131 3195 3207 710
Size: 1132 1289 64 12 4213
Fragments arranged by size:
4213 1289 1132 64 12
Bsp1286I G_dGCh'C
Cuts at: 370 1004 2154 2170 2243 2669 3574 3724 4335
Size: 634 1150 16 73 426 905 150 611
Cuts at: 4335 4428 4502 4692 4754 5484 370
Size: 93 74 190 62 730 1596
Fragments arranged by size:
1596 1150 905 730 634 611 426 190
150 93 74 73 62 16
BspEI T'CCGG_A
Cuts at: 3121 5930 3121
Size: 2809 3901
BspHI T'CATG_A
Cuts at: 5886 5886
Size: 6710
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3760 3974 4276 4657 3760
Size: 214 302 381 5813
Fragments arranged by size:
5813 381 302 214
BsrBI CCG'CTC
Cuts at: 50 432 5002 5056 6540 6705 50
Size: 382 4570 54 1484 165 55
Fragments arranged by size:
4570 1484 382 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3111 4622 3111
Size: 1511 5199
BsrFI r'CCGG_y
Cuts at: 2100 2225 4708 4889 5098 2100
Size: 125 2483 181 209 3712
Fragments arranged by size:
3712 2483 209 181 125
BsrGI T'GTAC_A
Cuts at: 2117 2117
Size: 6710
BssSI C'ACGA_G
Cuts at: 4981 5339 4981
Size: 358 6352
BstAPI GCAn_nnn'nTGC
Cuts at: 2200 2203 4087 2200
Size: 3 1884 4823
Fragments arranged by size:
4823 1884 3
BstBI TT'CG_AA
Cuts at: 110 5071 110
Size: 4961 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1065 2532 1065
Size: 1467 5243
BstYI r'GATC_y
Cuts at: 1 1120 1470 2126 3735 4202 4225 4560 4806
Size: 1119 350 656 1609 467 23 335 246
Cuts at: 4806 5818 5904 5916 6262 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1609 1119 1012 656 467 449 350 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1579 1768 2215 2278 1579
Size: 189 447 63 6011
Fragments arranged by size:
6011 447 189 63
BtgI C'CryG_G
Cuts at: 125 3185 125
Size: 3060 3650
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 148 1166 4660 4841 5004 148
Size: 1018 3494 181 163 1854
Fragments arranged by size:
3494 1854 1018 181 163
BtsI GCAGTG_nn'
Cuts at: 1042 1932 2131 3379 3953 1042
Size: 890 199 1248 574 3799
Fragments arranged by size:
3799 1248 890 574 199
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 524 559 524
Size: 35 6675
DraI TTT'AAA
Cuts at: 1110 2397 3709 1110
Size: 1287 1312 4111
Fragments arranged by size:
4111 1312 1287
DraIII CAC_nnn'GTG
Cuts at: 3018 3018
Size: 6710
DrdI GACnn_nn'nnGTC
Cuts at: 195 4416 5274 195
Size: 4221 858 1631
Fragments arranged by size:
4221 1631 858
EaeI y'GGCC_r
Cuts at: 393 467 617 1947 2522 3251 4295 4469 4860
Size: 74 150 1330 575 729 1044 174 391
Cuts at: 4860 4887 6232 393
Size: 27 1345 871
Fragments arranged by size:
1345 1330 1044 871 729 575 391 174
150 74 27
EagI C'GGCC_G
Cuts at: 4295 4295
Size: 6710
EarI CTCTTCn'nnn_
Cuts at: 186 700 872 1511 1804 3224 3302 3579 4733
Size: 514 172 639 293 1420 78 277 1154
Cuts at: 4733 4943 186
Size: 210 1953
Fragments arranged by size:
1953 1420 1154 639 514 293 277 210
172 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4918 5228 5374 6520 4918
Size: 310 146 1146 5108
Fragments arranged by size:
5108 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3572 3722 3572
Size: 150 6560
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 617 649 979 1072 1302 1394 1680 1785
Size: 214 32 330 93 230 92 286 105
Cuts at: 1785 2356 2374 3440 4535 4967 5714 5982 403
Size: 571 18 1066 1095 432 747 268 1131
Fragments arranged by size:
1131 1095 1066 747 571 432 330 286
268 230 214 105 93 92 32 18
EcoNI CCTnn'n_nnAGG
Cuts at: 1199 2277 2374 2703 1199
Size: 1078 97 329 5206
Fragments arranged by size:
5206 1078 329 97
EcoO109I rG'GnC_Cy
Cuts at: 1297 2150 2151 3331 3649 3849 1297
Size: 853 1 1180 318 200 4158
Fragments arranged by size:
4158 1180 853 318 200 1
EcoRI G'AATT_C
Cuts at: 777 1368 3714 777
Size: 591 2346 3773
Fragments arranged by size:
3773 2346 591
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2484 4729 2484
Size: 2245 4465
FspI TGC'GCA
Cuts at: 4491 4491
Size: 6710
HaeII r_GCGC'y
Cuts at: 177 266 2658 4144 4392 5414 6393 177
Size: 89 2392 1486 248 1022 979 494
Fragments arranged by size:
2392 1486 1022 979 494 248 89
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1384 1416 1772 1804 3027 3059 4552 4582 4584
Size: 32 356 32 1223 32 1493 30 2
Cuts at: 4584 4614 1384
Size: 30 3480
Fragments arranged by size:
3480 1493 1223 356 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 336 3749 336
Size: 3413 3297
HindIII A'AGCT_T
Cuts at: 2266 2466 2266
Size: 200 6510
KasI G'GCGC_C
Cuts at: 4140 4388 4140
Size: 248 6462
KpnI G_GTAC'C
Cuts at: 3730 3730
Size: 6710
MluI A'CGCG_T
Cuts at: 6143 6143
Size: 6710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5381 5565 5381
Size: 184 6526
MscI TGG'CCA
Cuts at: 469 619 1949 2524 3253 4471 469
Size: 150 1330 575 729 1218 2708
Fragments arranged by size:
2708 1330 1218 729 575 150
MslI CAynn'nnrTG
Cuts at: 363 2113 2530 4699 4826 363
Size: 1750 417 2169 127 2247
Fragments arranged by size:
2247 2169 1750 417 127
MspA1I CmG'CkG
Cuts at: 218 2191 2315 2741 3418 3821 4135 4495 5508
Size: 1973 124 426 677 403 314 360 1013
Cuts at: 5508 5753 218
Size: 245 1175
Fragments arranged by size:
1973 1175 1013 677 426 403 360 314
245 124
NaeI GCC'GGC
Cuts at: 4891 4891
Size: 6710
NarI GG'CG_CC
Cuts at: 4141 4389 4141
Size: 248 6462
NcoI C'CATG_G
Cuts at: 125 3185 125
Size: 3060 3650
NdeI CA'TA_TG
Cuts at: 915 915
Size: 6710
NgoMIV G'CCGG_C
Cuts at: 4889 4889
Size: 6710
NsiI A_TGCA'T
Cuts at: 3209 3209
Size: 6710
NspI r_CATG'y
Cuts at: 344 810 2393 3355 3763 4263 5170 344
Size: 466 1583 962 408 500 907 1884
Fragments arranged by size:
1884 1583 962 907 500 466 408
PciI A'CATG_T
Cuts at: 806 2389 5166 806
Size: 1583 2777 2350
Fragments arranged by size:
2777 2350 1583
PflMI CCAn_nnn'nTGG
Cuts at: 898 3191 3233 3614 898
Size: 2293 42 381 3994
Fragments arranged by size:
3994 2293 381 42
PfoI T'CCnGG_A
Cuts at: 278 995 1449 2372 3470 278
Size: 717 454 923 1098 3518
Fragments arranged by size:
3518 1098 923 717 454
PmeI GTTT'AAAC
Cuts at: 3709 3709
Size: 6710
PmlI CAC'GTG
Cuts at: 2493 2493
Size: 6710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5849 5881 5849
Size: 32 6678
PpuMI rG'GwC_Cy
Cuts at: 1297 3331 1297
Size: 2034 4676
PspOMI G'GGCC_C
Cuts at: 2150 2150
Size: 6710
PstI C_TGCA'G
Cuts at: 1074 1348 2196 3757 4442 1074
Size: 274 848 1561 685 3342
Fragments arranged by size:
3342 1561 848 685 274
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6710
PvuII CAG'CTG
Cuts at: 218 2191 2315 2741 3418 4135 4495 218
Size: 1973 124 426 677 717 360 2433
Fragments arranged by size:
2433 1973 717 677 426 360 124
RsrII CG'GwC_CG
Cuts at: 4905 4905
Size: 6710
SacI G_AGCT'C
Cuts at: 3574 3724 3574
Size: 150 6560
SalI G'TCGA_C
Cuts at: 3747 3747
Size: 6710
SapI GCTCTTCn'nnn_
Cuts at: 700 3579 4733 4943 700
Size: 2879 1154 210 2467
Fragments arranged by size:
2879 2467 1154 210
SbfI CC_TGCA'GG
Cuts at: 3757 3757
Size: 6710
ScaI AGT'ACT
Cuts at: 6227 6227
Size: 6710
SfcI C'TryA_G
Cuts at: 33 1070 1344 2192 3489 3653 3753 4438 5431
Size: 1037 274 848 1297 164 100 685 993
Cuts at: 5431 5622 33
Size: 191 1121
Fragments arranged by size:
1297 1121 1037 993 848 685 274 191
164 100
SfoI GGC'GCC
Cuts at: 4142 4390 4142
Size: 248 6462
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6710
SgrAI Cr'CCGG_yG
Cuts at: 2225 2225
Size: 6710
SmaI CCC'GGG
Cuts at: 3732 3732
Size: 6710
SmlI C'TyrA_G
Cuts at: 2632 3408 3523 3865 4780 5272 5534 2632
Size: 776 115 342 915 492 262 3808
Fragments arranged by size:
3808 915 776 492 342 262 115
SnaBI TAC'GTA
Cuts at: 3702 3702
Size: 6710
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6710
SphI G_CATG'C
Cuts at: 3763 4263 3763
Size: 500 6210
SspI AAT'ATT
Cuts at: 1233 1446 1233
Size: 213 6497
StuI AGG'CCT
Cuts at: 416 2021 416
Size: 1605 5105
StyI C'CwwG_G
Cuts at: 125 731 2641 2917 3185 3589 3844 4198 125
Size: 606 1910 276 268 404 255 354 2637
Fragments arranged by size:
2637 1910 606 404 354 276 268 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2187 3042 4293 4426 4885 5094 2187
Size: 855 1251 133 459 209 3803
Fragments arranged by size:
3803 1251 855 459 209 133
TatI w'GTAC_w
Cuts at: 1383 2117 6225 1383
Size: 734 4108 1868
Fragments arranged by size:
4108 1868 734
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2477 2509 6227 6259 2477
Size: 32 3718 32 2928
Fragments arranged by size:
3718 2928 32 32
Tth111I GACn'n_nGTC
Cuts at: 1972 4507 1972
Size: 2535 4175
XbaI T'CTAG_A
Cuts at: 681 3621 3741 681
Size: 2940 120 3650
Fragments arranged by size:
3650 2940 120
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1263 1676 4205 1263
Size: 413 2529 3768
Fragments arranged by size:
3768 2529 413
XmaI C'CCGG_G
Cuts at: 3730 3730
Size: 6710
XmnI GAAnn'nnTTC
Cuts at: 2069 2500 2715 2069
Size: 431 215 6064
Fragments arranged by size:
6064 431 215
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AhdI AleI AlfI AloI AscI AvrII
BaeI BcgI BglI BmgBI BmtI BsiWI BsmBI BssHII
BstEII BstZ17I ClaI EcoRV FseI FspAI HpaI MfeI
NheI NotI NruI PacI PasI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SrfI SwaI XhoI
ZraI