Restriction map for
pF1KSDA1392
(Circular) (Six-base) MAPSORT of: pF1KSDA1392.seq Check: 1526 from: 1 to: 5915
pF1KSDA1392 5915 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2325 2325
Size: 5915
AccI GT'mk_AC
Cuts at: 2953 2953
Size: 5915
Acc65I G'GTAC_C
Cuts at: 596 2931 596
Size: 2335 3580
AclI AA'CG_TT
Cuts at: 5755 5755
Size: 5915
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1022 1685 2162 3740 4172 4919 5187 1022
Size: 663 477 1578 432 747 268 1750
Fragments arranged by size:
1750 1578 747 663 477 432 268
AfeI AGC'GCT
Cuts at: 1830 1830
Size: 5915
AflIII A'CryG_T
Cuts at: 3267 4371 5348 3267
Size: 1104 977 3834
Fragments arranged by size:
3834 1104 977
AgeI A'CCGG_T
Cuts at: 623 4303 623
Size: 3680 2235
AhdI GACnn_n'nnGTC
Cuts at: 1557 1742 2164 1557
Size: 185 422 5308
Fragments arranged by size:
5308 422 185
AleI CACnn'nnGTG
Cuts at: 1408 2177 1408
Size: 769 5146
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 166 2195 166
Size: 2029 3886
AlwNI CAG_nnn'CTG
Cuts at: 3220 4787 3220
Size: 1567 4348
ApaI G_GGCC'C
Cuts at: 2119 2119
Size: 5915
ApaLI G'TGCA_C
Cuts at: 633 742 1525 4685 633
Size: 109 783 3160 1863
Fragments arranged by size:
3160 1863 783 109
ApoI r'AATT_y
Cuts at: 467 858 919 2575 2919 5268 5524 467
Size: 391 61 1656 344 2349 256 858
Fragments arranged by size:
2349 1656 858 391 344 256 61
AseI AT'TA_AT
Cuts at: 20 20
Size: 5915
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5915
AvaI C'yCGr_G
Cuts at: 346 649 1046 1414 1861 2542 2714 2935 346
Size: 303 397 368 447 681 172 221 3326
Fragments arranged by size:
3326 681 447 397 368 303 221 172
BamHI G'GATC_C
Cuts at: 2940 2940
Size: 5915
BanI G'GyrC_C
Cuts at: 596 825 1341 1962 2179 2524 2931 3345 3593
Size: 229 516 621 217 345 407 414 248
Cuts at: 3593 3628 596
Size: 35 2883
Fragments arranged by size:
2883 621 516 414 407 345 248 229
217 35
BanII G_rGCy'C
Cuts at: 1758 2119 2189 2785 2837 2929 3959 1758
Size: 361 70 596 52 92 1030 3714
Fragments arranged by size:
3714 1030 596 361 92 70 52
BbeI G_GCGC'C
Cuts at: 3349 3597 3349
Size: 248 5667
BbsI GAAGACnn'nnnn_
Cuts at: 139 743 1661 2317 139
Size: 604 918 656 3737
Fragments arranged by size:
3737 918 656 604
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 365 399 692 726 365
Size: 34 293 34 5554
Fragments arranged by size:
5554 293 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 608 3813 4574 5915 608
Size: 3205 761 1341 608
Fragments arranged by size:
3205 1341 761 608
BclI T'GATC_A
Cuts at: 3435 3435
Size: 5915
BglI GCCn_nnn'nGGC
Cuts at: 2079 2079
Size: 5915
BglII A'GATC_T
Cuts at: 509 3430 5467 509
Size: 2921 2037 957
Fragments arranged by size:
2921 2037 957
BlpI GC'TnA_GC
Cuts at: 3027 3027
Size: 5915
Bme1580I G_kGCm'C
Cuts at: 637 746 1346 1529 2084 2119 3540 3633 4689
Size: 109 600 183 555 35 1421 93 1056
Cuts at: 4689 637
Size: 1863
Fragments arranged by size:
1863 1421 1056 600 555 183 109 93
35
BmgBI CAC'GTC
Cuts at: 741 741
Size: 5915
BmrI ACTGGGnnnn_n'
Cuts at: 984 3127 3208 3543 5687 984
Size: 2143 81 335 2144 1212
Fragments arranged by size:
2144 2143 1212 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1226 4282 1226
Size: 3056 2859
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 506 2049 506
Size: 1543 4372
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3091 3970 4462 4760 3091
Size: 879 492 298 4246
Fragments arranged by size:
4246 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 632 1244 1262 5619 632
Size: 612 18 4357 928
Fragments arranged by size:
4357 928 612 18
BsaAI yAC'GTr
Cuts at: 434 2710 2907 3268 434
Size: 2276 197 361 3081
Fragments arranged by size:
3081 2276 361 197
BsaBI GATnn'nnATC
Cuts at: 6 1063 3429 5466 6
Size: 1057 2366 2037 455
Fragments arranged by size:
2366 2037 1057 455
BsaHI Gr'CG_yC
Cuts at: 1648 2160 3346 3594 4296 5792 1648
Size: 512 1186 248 702 1496 1771
Fragments arranged by size:
1771 1496 1186 702 512 248
BsaWI w'CCGG_w
Cuts at: 623 938 1553 3104 3625 4303 4577 4724 5135
Size: 315 615 1551 521 678 274 147 411
Cuts at: 5135 623
Size: 1403
Fragments arranged by size:
1551 1403 678 615 521 411 315 274
147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1170 2052 2104 2147 2284 2512 2515 2530 1170
Size: 882 52 43 137 228 3 15 4555
Fragments arranged by size:
4555 882 228 137 52 43 15 3
BseYI C'CCAG_C
Cuts at: 1299 1693 2188 2396 3340 4098 4675 5171 1299
Size: 394 495 208 944 758 577 496 2043
Fragments arranged by size:
2043 944 758 577 496 495 394 208
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 1715 217
Size: 1498 4417
BsiEI CG_ry'CG
Cuts at: 104 3457 3503 4711 104
Size: 3353 46 1208 1308
Fragments arranged by size:
3353 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 145 425 637 746 1415 1529 1958 2929 3707
Size: 280 212 109 669 114 429 971 778
Cuts at: 3707 3897 4689 145
Size: 190 792 1371
Fragments arranged by size:
1371 971 792 778 669 429 280 212
190 114 109
BsmBI CGTCTCn'nnnn_
Cuts at: 1013 1942 2457 1013
Size: 929 515 4471
Fragments arranged by size:
4471 929 515
Bsp1286I G_dGCh'C
Cuts at: 145 425 637 746 1346 1415 1529 1758 1958
Size: 280 212 109 600 69 114 229 200
Cuts at: 1958 2084 2119 2189 2785 2837 2929 3540 3633
Size: 126 35 70 596 52 92 611 93
Cuts at: 3633 3707 3897 3959 4689 145
Size: 74 190 62 730 1371
Fragments arranged by size:
1371 730 611 600 596 280 229 212
200 190 126 114 109 93 92 74
70 69 62 52 35
BspEI T'CCGG_A
Cuts at: 5135 5135
Size: 5915
BspHI T'CATG_A
Cuts at: 1124 5091 1124
Size: 3967 1948
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2040 2325 2965 3179 3481 3862 2040
Size: 285 640 214 302 381 4093
Fragments arranged by size:
4093 640 381 302 285 214
BsrBI CCG'CTC
Cuts at: 50 2547 4207 4261 5745 5910 50
Size: 2497 1660 54 1484 165 55
Fragments arranged by size:
2497 1660 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 383 694 2326 3827 383
Size: 311 1632 1501 2471
Fragments arranged by size:
2471 1632 1501 311
BsrFI r'CCGG_y
Cuts at: 623 3913 4094 4303 623
Size: 3290 181 209 2235
Fragments arranged by size:
3290 2235 209 181
BssSI C'ACGA_G
Cuts at: 4186 4544 4186
Size: 358 5557
BstAPI GCAn_nnn'nTGC
Cuts at: 3292 3292
Size: 5915
BstBI TT'CG_AA
Cuts at: 110 4276 110
Size: 4166 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 972 1605 2176 2196 972
Size: 633 571 20 4691
Fragments arranged by size:
4691 633 571 20
BstYI r'GATC_y
Cuts at: 1 353 509 1573 1594 2940 3407 3430 3765
Size: 352 156 1064 21 1346 467 23 335
Cuts at: 3765 4011 5023 5109 5121 5467 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1346 1064 1012 467 449 352 346 335
246 156 86 23 21 12
Bsu36I CC'TnA_GG
Cuts at: 672 672
Size: 5915
BtgI C'CryG_G
Cuts at: 248 1094 1224 2175 2790 248
Size: 846 130 951 615 3373
Fragments arranged by size:
3373 951 846 615 130
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3865 4046 4209 3865
Size: 181 163 5571
Fragments arranged by size:
5571 181 163
BtsI GCAGTG_nn'
Cuts at: 708 723 2539 3158 708
Size: 15 1816 619 3465
Fragments arranged by size:
3465 1816 619 15
DraI TTT'AAA
Cuts at: 951 2914 951
Size: 1963 3952
DrdI GACnn_nn'nnGTC
Cuts at: 1257 3621 4479 1257
Size: 2364 858 2693
Fragments arranged by size:
2693 2364 858
EaeI y'GGCC_r
Cuts at: 365 974 3500 3674 4065 4092 5437 365
Size: 609 2526 174 391 27 1345 843
Fragments arranged by size:
2526 1345 843 609 391 174 27
EagI C'GGCC_G
Cuts at: 3500 3500
Size: 5915
EarI CTCTTCn'nnn_
Cuts at: 998 2249 3938 4148 998
Size: 1251 1689 210 2765
Fragments arranged by size:
2765 1689 1251 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4123 4433 4579 5725 4123
Size: 310 146 1146 4313
Fragments arranged by size:
4313 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2927 2927
Size: 5915
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 506 1022 1685 2049 2162 3740 4172 4919 5187
Size: 516 663 364 113 1578 432 747 268
Cuts at: 5187 506
Size: 1234
Fragments arranged by size:
1578 1234 747 663 516 432 364 268
113
EcoO109I rG'GnC_Cy
Cuts at: 206 1169 1621 2052 2115 2116 2366 3054 206
Size: 963 452 431 63 1 250 688 3067
Fragments arranged by size:
3067 963 688 452 431 250 63 1
EcoRI G'AATT_C
Cuts at: 2919 2919
Size: 5915
EcoRV GAT'ATC
Cuts at: 1296 1296
Size: 5915
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3934 3934
Size: 5915
FspI TGC'GCA
Cuts at: 3696 3696
Size: 5915
HaeII r_GCGC'y
Cuts at: 1832 3349 3597 4619 5598 1832
Size: 1517 248 1022 979 2149
Fragments arranged by size:
2149 1517 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 175 207 864 896 1543 1575 1728 1760 2150
Size: 32 657 32 647 32 153 32 390
Cuts at: 2150 2182 2452 2484 3757 3787 3789 3819 175
Size: 32 270 32 1273 30 2 30 2271
Fragments arranged by size:
2271 1273 657 647 390 270 153 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2954 2954
Size: 5915
HindIII A'AGCT_T
Cuts at: 2885 2885
Size: 5915
KasI G'GCGC_C
Cuts at: 3345 3593 3345
Size: 248 5667
KpnI G_GTAC'C
Cuts at: 600 2935 600
Size: 2335 3580
MluI A'CGCG_T
Cuts at: 5348 5348
Size: 5915
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 475 1301 1601 1924 2756 2897 4586 4770 475
Size: 826 300 323 832 141 1689 184 1620
Fragments arranged by size:
1689 1620 832 826 323 300 184 141
MscI TGG'CCA
Cuts at: 367 976 3676 367
Size: 609 2700 2606
Fragments arranged by size:
2700 2606 609
MslI CAynn'nnrTG
Cuts at: 310 1408 1440 2177 2683 3904 4031 310
Size: 1098 32 737 506 1221 127 2194
Fragments arranged by size:
2194 1221 1098 737 506 127 32
MspA1I CmG'CkG
Cuts at: 250 972 1187 1303 2122 3026 3340 3700 4713
Size: 722 215 116 819 904 314 360 1013
Cuts at: 4713 4958 250
Size: 245 1207
Fragments arranged by size:
1207 1013 904 819 722 360 314 245
215 116
NaeI GCC'GGC
Cuts at: 4096 4096
Size: 5915
NarI GG'CG_CC
Cuts at: 3346 3594 3346
Size: 248 5667
NcoI C'CATG_G
Cuts at: 1224 2175 2790 1224
Size: 951 615 4349
Fragments arranged by size:
4349 951 615
NgoMIV G'CCGG_C
Cuts at: 4094 4094
Size: 5915
NspI r_CATG'y
Cuts at: 757 2968 3468 4375 757
Size: 2211 500 907 2297
Fragments arranged by size:
2297 2211 907 500
PasI CC'CwG_GG
Cuts at: 1346 2361 1346
Size: 1015 4900
PciI A'CATG_T
Cuts at: 4371 4371
Size: 5915
PflMI CCAn_nnn'nTGG
Cuts at: 1879 2534 1879
Size: 655 5260
PfoI T'CCnGG_A
Cuts at: 1561 1561
Size: 5915
PmeI GTTT'AAAC
Cuts at: 2914 2914
Size: 5915
PmlI CAC'GTG
Cuts at: 434 434
Size: 5915
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5054 5086 5054
Size: 32 5883
PpuMI rG'GwC_Cy
Cuts at: 206 1169 2052 2366 206
Size: 963 883 314 3755
Fragments arranged by size:
3755 963 883 314
PspOMI G'GGCC_C
Cuts at: 2115 2115
Size: 5915
PspXI vC'TCGA_Gb
Cuts at: 1414 2542 1414
Size: 1128 4787
PstI C_TGCA'G
Cuts at: 733 1675 2962 3647 733
Size: 942 1287 685 3001
Fragments arranged by size:
3001 1287 942 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5915
PvuII CAG'CTG
Cuts at: 972 1303 3340 3700 972
Size: 331 2037 360 3187
Fragments arranged by size:
3187 2037 360 331
RsrII CG'GwC_CG
Cuts at: 183 195 4110 183
Size: 12 3915 1988
Fragments arranged by size:
3915 1988 12
SacI G_AGCT'C
Cuts at: 2929 2929
Size: 5915
SacII CC_GC'GG
Cuts at: 251 251
Size: 5915
SalI G'TCGA_C
Cuts at: 2952 2952
Size: 5915
SapI GCTCTTCn'nnn_
Cuts at: 3938 4148 3938
Size: 210 5705
SbfI CC_TGCA'GG
Cuts at: 2962 2962
Size: 5915
ScaI AGT'ACT
Cuts at: 852 1719 5432 852
Size: 867 3713 1335
Fragments arranged by size:
3713 1335 867
SfcI C'TryA_G
Cuts at: 33 729 1671 2860 2958 3643 4636 4827 33
Size: 696 942 1189 98 685 993 191 1121
Fragments arranged by size:
1189 1121 993 942 696 685 191 98
SfoI GGC'GCC
Cuts at: 3347 3595 3347
Size: 248 5667
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5915
SmaI CCC'GGG
Cuts at: 651 2937 651
Size: 2286 3629
SmlI C'TyrA_G
Cuts at: 1414 2542 3070 3985 4477 4739 1414
Size: 1128 528 915 492 262 2590
Fragments arranged by size:
2590 1128 915 528 492 262
SnaBI TAC'GTA
Cuts at: 2907 2907
Size: 5915
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5915
SphI G_CATG'C
Cuts at: 757 2968 3468 757
Size: 2211 500 3204
Fragments arranged by size:
3204 2211 500
StuI AGG'CCT
Cuts at: 1728 1728
Size: 5915
StyI C'CwwG_G
Cuts at: 782 1224 1586 2175 2790 3049 3403 782
Size: 442 362 589 615 259 354 3294
Fragments arranged by size:
3294 615 589 442 362 354 259
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 318 418 774 846 1226 1618 2656 3498 3631
Size: 100 356 72 380 392 1038 842 133
Cuts at: 3631 4090 4299 318
Size: 459 209 1934
Fragments arranged by size:
1934 1038 842 459 392 380 356 209
133 100 72
TatI w'GTAC_w
Cuts at: 850 1717 5430 850
Size: 867 3713 1335
Fragments arranged by size:
3713 1335 867
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1750 1782 5432 5464 1750
Size: 32 3650 32 2201
Fragments arranged by size:
3650 2201 32 32
Tth111I GACn'n_nGTC
Cuts at: 3712 3712
Size: 5915
XbaI T'CTAG_A
Cuts at: 2946 2946
Size: 5915
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1623 2073 2797 3410 1623
Size: 450 724 613 4128
Fragments arranged by size:
4128 724 613 450
XhoI C'TCGA_G
Cuts at: 1414 2542 1414
Size: 1128 4787
XmaI C'CCGG_G
Cuts at: 649 2935 649
Size: 2286 3629
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AflII AloI AscI AvrII BaeI BbvCI BfrBI
BmtI Bpu10I BsaXI BsiWI BsmI BsrGI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoNI FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PshAI
PsiI PsrI SanDI SexAI SfiI SgrAI SrfI SspI
SwaI XmnI ZraI