Restriction map for
pF1KSDA1394
(Circular) (Six-base) MAPSORT of: pF1KSDA1394.seq Check: 7860 from: 1 to: 6035
pF1KSDA1394 6035 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1188 1383 2203 2789 1188
Size: 195 820 586 4434
Fragments arranged by size:
4434 820 586 195
AccI GT'mk_AC
Cuts at: 365 3073 365
Size: 2708 3327
Acc65I G'GTAC_C
Cuts at: 2175 3051 2175
Size: 876 5159
AclI AA'CG_TT
Cuts at: 5875 5875
Size: 6035
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3860 4292 5039 5307 3860
Size: 432 747 268 4588
Fragments arranged by size:
4588 747 432 268
AflIII A'CryG_T
Cuts at: 3387 4491 5468 3387
Size: 1104 977 3954
Fragments arranged by size:
3954 1104 977
AgeI A'CCGG_T
Cuts at: 4423 4423
Size: 6035
AleI CACnn'nnGTG
Cuts at: 2834 2834
Size: 6035
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 517 2353 517
Size: 1836 4199
AlwNI CAG_nnn'CTG
Cuts at: 831 1046 1731 2781 2790 2826 2970 3340 4907
Size: 215 685 1050 9 36 144 370 1567
Cuts at: 4907 831
Size: 1959
Fragments arranged by size:
1959 1567 1050 685 370 215 144 36
9
ApaI G_GGCC'C
Cuts at: 247 2987 247
Size: 2740 3295
ApaLI G'TGCA_C
Cuts at: 2167 4805 2167
Size: 2638 3397
ApoI r'AATT_y
Cuts at: 3039 5388 5644 3039
Size: 2349 256 3430
Fragments arranged by size:
3430 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6035
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6035
AvaI C'yCGr_G
Cuts at: 199 334 350 534 589 2279 3055 199
Size: 135 16 184 55 1690 776 3179
Fragments arranged by size:
3179 1690 776 184 135 55 16
AvrII C'CTAG_G
Cuts at: 918 918
Size: 6035
BamHI G'GATC_C
Cuts at: 186 328 2648 3060 186
Size: 142 2320 412 3161
Fragments arranged by size:
3161 2320 412 142
BanI G'GyrC_C
Cuts at: 299 435 579 1325 1493 1668 2175 2362 2487
Size: 136 144 746 168 175 507 187 125
Cuts at: 2487 2874 3051 3465 3713 3748 299
Size: 387 177 414 248 35 2586
Fragments arranged by size:
2586 746 507 414 387 248 187 177
175 168 144 136 125 35
BanII G_rGCy'C
Cuts at: 223 247 262 487 535 1796 1929 2576 2660
Size: 24 15 225 48 1261 133 647 84
Cuts at: 2660 2897 2987 3049 4079 223
Size: 237 90 62 1030 2179
Fragments arranged by size:
2179 1261 1030 647 237 225 133 90
84 62 48 24 15
BbeI G_GCGC'C
Cuts at: 303 1497 1672 3469 3717 303
Size: 1194 175 1797 248 2621
Fragments arranged by size:
2621 1797 1194 248 175
BbsI GAAGACnn'nnnn_
Cuts at: 2553 2553
Size: 6035
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1746 1780 1746
Size: 34 6001
BciVI GTATCCnnnnn_n'
Cuts at: 3933 4694 6035 3933
Size: 761 1341 3933
Fragments arranged by size:
3933 1341 761
BclI T'GATC_A
Cuts at: 3555 3555
Size: 6035
BglI GCCn_nnn'nGGC
Cuts at: 218 891 1441 1453 1657 2481 2954 218
Size: 673 550 12 204 824 473 3299
Fragments arranged by size:
3299 824 673 550 473 204 12
BglII A'GATC_T
Cuts at: 3550 5587 3550
Size: 2037 3998
BlpI GC'TnA_GC
Cuts at: 785 1279 2045 3147 785
Size: 494 766 1102 3673
Fragments arranged by size:
3673 1102 766 494
Bme1580I G_kGCm'C
Cuts at: 133 247 440 2126 2171 2365 2987 3660 3753
Size: 114 193 1686 45 194 622 673 93
Cuts at: 3753 4809 133
Size: 1056 1359
Fragments arranged by size:
1686 1359 1056 673 622 194 193 114
93 45
BmgBI CAC'GTC
Cuts at: 309 2376 309
Size: 2067 3968
BmrI ACTGGGnnnn_n'
Cuts at: 226 1362 1555 1833 1971 3247 3328 3663 5807
Size: 1136 193 278 138 1276 81 335 2144
Cuts at: 5807 226
Size: 454
Fragments arranged by size:
2144 1276 1136 454 335 278 193 138
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4402 4402
Size: 6035
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 535 823 1150 1159 1372 1480 1591 1615
Size: 282 288 327 9 213 108 111 24
Cuts at: 1615 1633 1780 2158 2221 2674 2881 253
Size: 18 147 378 63 453 207 3407
Fragments arranged by size:
3407 453 378 327 288 282 213 207
147 111 108 63 24 18 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1921 2560 3211 4090 4582 4880 1921
Size: 639 651 879 492 298 3076
Fragments arranged by size:
3076 879 651 639 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1675 2806 5739 1675
Size: 1131 2933 1971
Fragments arranged by size:
2933 1971 1131
BsaAI yAC'GTr
Cuts at: 2734 3027 3388 2734
Size: 293 361 5381
Fragments arranged by size:
5381 361 293
BsaBI GATnn'nnATC
Cuts at: 6 3549 5586 6
Size: 3543 2037 455
Fragments arranged by size:
3543 2037 455
BsaHI Gr'CG_yC
Cuts at: 300 1074 1494 1669 1751 2756 3466 3714 4416
Size: 774 420 175 82 1005 710 248 702
Cuts at: 4416 5912 300
Size: 1496 423
Fragments arranged by size:
1496 1005 774 710 702 423 420 248
175 82
BsaWI w'CCGG_w
Cuts at: 411 1880 3224 3745 4423 4697 4844 5255 411
Size: 1469 1344 521 678 274 147 411 1191
Fragments arranged by size:
1469 1344 1191 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1156 1186 1318 1348 1606 1636 1156
Size: 30 132 30 258 30 5555
Fragments arranged by size:
5555 258 132 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1156 1159 1162 1399 1906 2878 2905 1156
Size: 3 3 237 507 972 27 4286
Fragments arranged by size:
4286 972 507 237 27 3 3
BseYI C'CCAG_C
Cuts at: 490 836 1095 1184 1638 2025 2063 2319 2324
Size: 346 259 89 454 387 38 256 5
Cuts at: 2324 2590 2774 2923 2952 2986 3460 4218 4795
Size: 266 184 149 29 34 474 758 577
Cuts at: 4795 5291 490
Size: 496 1234
Fragments arranged by size:
1234 758 577 496 474 454 387 346
266 259 256 184 149 89 38 34
29 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1063 1121 1786 2053 2237 2812 1063
Size: 58 665 267 184 575 4286
Fragments arranged by size:
4286 665 575 267 184 58
BsiEI CG_ry'CG
Cuts at: 104 1430 1544 1652 1699 2835 3577 3623 4831
Size: 1326 114 108 47 1136 742 46 1208
Cuts at: 4831 104
Size: 1308
Fragments arranged by size:
1326 1308 1208 1136 742 114 108 47
46
BsiHKAI G_wGCw'C
Cuts at: 1880 1982 2171 2264 2372 2576 2660 2739 3049
Size: 102 189 93 108 204 84 79 310
Cuts at: 3049 3827 4017 4809 1880
Size: 778 190 792 3106
Fragments arranged by size:
3106 792 778 310 204 190 189 108
102 93 84 79
BsmBI CGTCTCn'nnnn_
Cuts at: 955 2452 2947 955
Size: 1497 495 4043
Fragments arranged by size:
4043 1497 495
Bsp1286I G_dGCh'C
Cuts at: 133 223 247 262 440 487 535 1796 1880
Size: 90 24 15 178 47 48 1261 84
Cuts at: 1880 1929 1982 2126 2171 2264 2365 2372 2576
Size: 49 53 144 45 93 101 7 204
Cuts at: 2576 2660 2739 2897 2987 3049 3660 3753 3827
Size: 84 79 158 90 62 611 93 74
Cuts at: 3827 4017 4079 4809 133
Size: 190 62 730 1359
Fragments arranged by size:
1359 1261 730 611 204 190 178 158
144 101 93 93 90 90 84 84
79 74 62 62 53 49 48 47
45 24 15 7
BspEI T'CCGG_A
Cuts at: 411 5255 411
Size: 4844 1191
BspHI T'CATG_A
Cuts at: 2192 5211 2192
Size: 3019 3016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 772 1014 1188 1383 2203 2645 2681 2789 3085
Size: 242 174 195 820 442 36 108 296
Cuts at: 3085 3299 3601 3982 772
Size: 214 302 381 2825
Fragments arranged by size:
2825 820 442 381 302 296 242 214
195 174 108 36
BsrBI CCG'CTC
Cuts at: 50 4327 4381 5865 6030 50
Size: 4277 54 1484 165 55
Fragments arranged by size:
4277 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2337 3947 2337
Size: 1610 4425
BsrFI r'CCGG_y
Cuts at: 809 1465 1543 1644 4033 4214 4423 809
Size: 656 78 101 2389 181 209 2421
Fragments arranged by size:
2421 2389 656 209 181 101 78
BssHII G'CGCG_C
Cuts at: 1607 1702 1780 1607
Size: 95 78 5862
Fragments arranged by size:
5862 95 78
BssSI C'ACGA_G
Cuts at: 1807 2738 4306 4664 1807
Size: 931 1568 358 3178
Fragments arranged by size:
3178 1568 931 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2076 2826 3412 2076
Size: 750 586 4699
Fragments arranged by size:
4699 750 586
BstBI TT'CG_AA
Cuts at: 110 4396 110
Size: 4286 1749
BstEII G'GTnAC_C
Cuts at: 465 465
Size: 6035
BstXI CCAn_nnnn'nTGG
Cuts at: 231 844 2040 2484 231
Size: 613 1196 444 3782
Fragments arranged by size:
3782 1196 613 444
BstYI r'GATC_y
Cuts at: 1 186 328 2648 3060 3527 3550 3885 4131
Size: 185 142 2320 412 467 23 335 246
Cuts at: 4131 5143 5229 5241 5587 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2320 1012 467 449 412 346 335 246
185 142 86 23 12
BtgI C'CryG_G
Cuts at: 426 1084 1305 1633 2085 2835 426
Size: 658 221 328 452 750 3626
Fragments arranged by size:
3626 750 658 452 328 221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3985 4166 4329 3985
Size: 181 163 5691
Fragments arranged by size:
5691 181 163
BtsI GCAGTG_nn'
Cuts at: 1109 1273 2225 2910 3278 1109
Size: 164 952 685 368 3866
Fragments arranged by size:
3866 952 685 368 164
ClaI AT'CG_AT
Cuts at: 2978 2978
Size: 6035
DraI TTT'AAA
Cuts at: 3034 3034
Size: 6035
DraIII CAC_nnn'GTG
Cuts at: 1264 1809 2376 1264
Size: 545 567 4923
Fragments arranged by size:
4923 567 545
DrdI GACnn_nn'nnGTC
Cuts at: 3741 4599 3741
Size: 858 5177
EaeI y'GGCC_r
Cuts at: 429 723 1226 1427 1442 1541 1649 2742 3620
Size: 294 503 201 15 99 108 1093 878
Cuts at: 3620 3794 4185 4212 5557 429
Size: 174 391 27 1345 907
Fragments arranged by size:
1345 1093 907 878 503 391 294 201
174 108 99 27 15
EagI C'GGCC_G
Cuts at: 1427 1541 1649 3620 1427
Size: 114 108 1971 3842
Fragments arranged by size:
3842 1971 114 108
EarI CTCTTCn'nnn_
Cuts at: 233 2047 4058 4268 233
Size: 1814 2011 210 2000
Fragments arranged by size:
2011 2000 1814 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 475 2935 4243 4553 4699 5845 475
Size: 2460 1308 310 146 1146 665
Fragments arranged by size:
2460 1308 1146 665 310 146
EcoICRI GAG'CTC
Cuts at: 2574 2658 3047 2574
Size: 84 389 5562
Fragments arranged by size:
5562 389 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 535 823 1150 1159 1372 1480 1591 1615
Size: 282 288 327 9 213 108 111 24
Cuts at: 1615 1633 1780 2158 2221 2674 2881 3860 4292
Size: 18 147 378 63 453 207 979 432
Cuts at: 4292 5039 5307 253
Size: 747 268 981
Fragments arranged by size:
981 979 747 453 432 378 327 288
282 268 213 207 147 111 108 63
24 18 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1162 1381 1714 2962 1162
Size: 219 333 1248 4235
Fragments arranged by size:
4235 1248 333 219
EcoO109I rG'GnC_Cy
Cuts at: 196 228 243 244 648 718 1005 2787 2823
Size: 32 15 1 404 70 287 1782 36
Cuts at: 2823 2868 2967 3174 196
Size: 45 99 207 3057
Fragments arranged by size:
3057 1782 404 287 207 99 70 45
36 32 15 1
EcoRI G'AATT_C
Cuts at: 3039 3039
Size: 6035
EcoRV GAT'ATC
Cuts at: 1017 1017
Size: 6035
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 424 4054 424
Size: 3630 2405
FseI GG_CCGG'CC
Cuts at: 1469 1469
Size: 6035
FspI TGC'GCA
Cuts at: 3816 3816
Size: 6035
HaeII r_GCGC'y
Cuts at: 303 1053 1126 1363 1497 1672 1745 3469 3717
Size: 750 73 237 134 175 73 1724 248
Cuts at: 3717 4739 5718 303
Size: 1022 979 620
Fragments arranged by size:
1724 1022 979 750 620 248 237 175
134 73 73
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3877 3907 3909 3939 3877
Size: 30 2 30 5973
Fragments arranged by size:
5973 30 30 2
HincII GTy'rAC
Cuts at: 2670 3074 2670
Size: 404 5631
KasI G'GCGC_C
Cuts at: 299 1493 1668 3465 3713 299
Size: 1194 175 1797 248 2621
Fragments arranged by size:
2621 1797 1194 248 175
KpnI G_GTAC'C
Cuts at: 2179 3055 2179
Size: 876 5159
MluI A'CGCG_T
Cuts at: 5468 5468
Size: 6035
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1234 2447 4706 4890 1234
Size: 1213 2259 184 2379
Fragments arranged by size:
2379 2259 1213 184
MscI TGG'CCA
Cuts at: 2744 3796 2744
Size: 1052 4983
MslI CAynn'nnrTG
Cuts at: 2084 2834 4024 4151 2084
Size: 750 1190 127 3968
Fragments arranged by size:
3968 1190 750 127
MspA1I CmG'CkG
Cuts at: 428 1086 1188 1307 1540 1635 1674 1728 1800
Size: 658 102 119 233 95 39 54 72
Cuts at: 1800 2029 2067 2528 3146 3460 3820 4833 5078
Size: 229 38 461 618 314 360 1013 245
Cuts at: 5078 428
Size: 1385
Fragments arranged by size:
1385 1013 658 618 461 360 314 245
233 229 119 102 95 72 54 39
38
NaeI GCC'GGC
Cuts at: 1467 1545 1646 4216 1467
Size: 78 101 2570 3286
Fragments arranged by size:
3286 2570 101 78
NarI GG'CG_CC
Cuts at: 300 1494 1669 3466 3714 300
Size: 1194 175 1797 248 2621
Fragments arranged by size:
2621 1797 1194 248 175
NcoI C'CATG_G
Cuts at: 2085 2085
Size: 6035
NgoMIV G'CCGG_C
Cuts at: 1465 1543 1644 4214 1465
Size: 78 101 2570 3286
Fragments arranged by size:
3286 2570 101 78
NspI r_CATG'y
Cuts at: 546 2475 3088 3588 4495 546
Size: 1929 613 500 907 2086
Fragments arranged by size:
2086 1929 907 613 500
PasI CC'CwG_GG
Cuts at: 248 643 1008 248
Size: 395 365 5275
Fragments arranged by size:
5275 395 365
PciI A'CATG_T
Cuts at: 4491 4491
Size: 6035
PflMI CCAn_nnn'nTGG
Cuts at: 309 2314 2751 2970 309
Size: 2005 437 219 3374
Fragments arranged by size:
3374 2005 437 219
PfoI T'CCnGG_A
Cuts at: 659 2269 2651 659
Size: 1610 382 4043
Fragments arranged by size:
4043 1610 382
PmeI GTTT'AAAC
Cuts at: 3034 3034
Size: 6035
PmlI CAC'GTG
Cuts at: 2734 2734
Size: 6035
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5174 5206 5174
Size: 32 6003
PpuMI rG'GwC_Cy
Cuts at: 196 228 196
Size: 32 6003
PspOMI G'GGCC_C
Cuts at: 243 2983 243
Size: 2740 3295
PstI C_TGCA'G
Cuts at: 386 398 1025 2020 2786 2822 2905 3082 3767
Size: 12 627 995 766 36 83 177 685
Cuts at: 3767 386
Size: 2654
Fragments arranged by size:
2654 995 766 685 627 177 83 36
12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6035
PvuII CAG'CTG
Cuts at: 1188 1728 1800 2029 2067 3460 3820 1188
Size: 540 72 229 38 1393 360 3403
Fragments arranged by size:
3403 1393 540 360 229 72 38
RsrII CG'GwC_CG
Cuts at: 4230 4230
Size: 6035
SacI G_AGCT'C
Cuts at: 2576 2660 3049 2576
Size: 84 389 5562
Fragments arranged by size:
5562 389 84
SacII CC_GC'GG
Cuts at: 429 1087 1308 1636 429
Size: 658 221 328 4828
Fragments arranged by size:
4828 658 328 221
SalI G'TCGA_C
Cuts at: 3072 3072
Size: 6035
SanDI GG'GwC_CC
Cuts at: 196 196
Size: 6035
SapI GCTCTTCn'nnn_
Cuts at: 2047 4058 4268 2047
Size: 2011 210 3814
Fragments arranged by size:
3814 2011 210
SbfI CC_TGCA'GG
Cuts at: 1025 2786 2822 3082 1025
Size: 1761 36 260 3978
Fragments arranged by size:
3978 1761 260 36
ScaI AGT'ACT
Cuts at: 5552 5552
Size: 6035
SexAI A'CCwGG_T
Cuts at: 2171 2381 2171
Size: 210 5825
SfcI C'TryA_G
Cuts at: 33 372 382 394 1021 2016 2782 2818 2901
Size: 339 10 12 627 995 766 36 83
Cuts at: 2901 3078 3763 4756 4947 33
Size: 177 685 993 191 1121
Fragments arranged by size:
1121 995 993 766 685 627 339 191
177 83 36 12 10
SfiI GGCCn_nnn'nGGCC
Cuts at: 1441 1441
Size: 6035
SfoI GGC'GCC
Cuts at: 301 1495 1670 3467 3715 301
Size: 1194 175 1797 248 2621
Fragments arranged by size:
2621 1797 1194 248 175
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6035
SmaI CCC'GGG
Cuts at: 352 536 591 3057 352
Size: 184 55 2466 3330
Fragments arranged by size:
3330 2466 184 55
SmlI C'TyrA_G
Cuts at: 1936 2575 3190 4105 4597 4859 1936
Size: 639 615 915 492 262 3112
Fragments arranged by size:
3112 915 639 615 492 262
SnaBI TAC'GTA
Cuts at: 3027 3027
Size: 6035
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6035
SphI G_CATG'C
Cuts at: 2475 3088 3588 2475
Size: 613 500 4922
Fragments arranged by size:
4922 613 500
SrfI GCCC'GGGC
Cuts at: 352 352
Size: 6035
StuI AGG'CCT
Cuts at: 707 707
Size: 6035
StyI C'CwwG_G
Cuts at: 224 623 918 2085 3169 3523 224
Size: 399 295 1167 1084 354 2736
Fragments arranged by size:
2736 1167 1084 399 354 295
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1000 1087 2077 2212 2321 2614 3618 3751 4210
Size: 87 990 135 109 293 1004 133 459
Cuts at: 4210 4419 1000
Size: 209 2616
Fragments arranged by size:
2616 1004 990 459 293 209 135 133
109 87
TatI w'GTAC_w
Cuts at: 5550 5550
Size: 6035
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 435 467 1248 1280 1318 1350 1606 1638 1819
Size: 32 781 32 38 32 256 32 181
Cuts at: 1819 1851 5552 5584 435
Size: 32 3701 32 886
Fragments arranged by size:
3701 886 781 256 181 38 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 670 3832 670
Size: 3162 2873
XbaI T'CTAG_A
Cuts at: 3066 3066
Size: 6035
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 696 2311 2343 3530 132
Size: 564 1615 32 1187 2637
Fragments arranged by size:
2637 1615 1187 564 32
XmaI C'CCGG_G
Cuts at: 350 534 589 3055 350
Size: 184 55 2466 3330
Fragments arranged by size:
3330 2466 184 55
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI BaeI BbvCI
BfrBI BmtI Bpu10I BsiWI BsmI BsrGI BstZ17I Bsu36I
CspCI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PshAI PsiI PspXI PsrI SgrAI
SspI SwaI XhoI XmnI ZraI