Restriction map for
pF1KSDA1397
(Circular) (Six-base) MAPSORT of: pF1KSDA1397.seq Check: 2958 from: 1 to: 5171
pF1KSDA1397 5171 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 458 495 684 458
Size: 37 189 4945
Fragments arranged by size:
4945 189 37
AatII G_ACGT'C
Cuts at: 1310 1310
Size: 5171
AccI GT'mk_AC
Cuts at: 2209 2209
Size: 5171
Acc65I G'GTAC_C
Cuts at: 2187 2187
Size: 5171
AclI AA'CG_TT
Cuts at: 5011 5011
Size: 5171
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1102 2996 3428 4175 4443 1102
Size: 1894 432 747 268 1830
Fragments arranged by size:
1894 1830 747 432 268
AflIII A'CryG_T
Cuts at: 637 2523 3627 4604 637
Size: 1886 1104 977 1204
Fragments arranged by size:
1886 1204 1104 977
AgeI A'CCGG_T
Cuts at: 3559 3559
Size: 5171
AleI CACnn'nnGTG
Cuts at: 1144 1144
Size: 5171
AlwNI CAG_nnn'CTG
Cuts at: 414 672 1030 1263 2476 4043 414
Size: 258 358 233 1213 1567 1542
Fragments arranged by size:
1567 1542 1213 358 258 233
ApaLI G'TGCA_C
Cuts at: 1002 3941 1002
Size: 2939 2232
ApoI r'AATT_y
Cuts at: 348 1945 2066 2175 4524 4780 348
Size: 1597 121 109 2349 256 739
Fragments arranged by size:
2349 1597 739 256 121 109
AseI AT'TA_AT
Cuts at: 20 2084 20
Size: 2064 3107
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5171
AvaI C'yCGr_G
Cuts at: 915 1793 2191 915
Size: 878 398 3895
Fragments arranged by size:
3895 878 398
BamHI G'GATC_C
Cuts at: 2196 2196
Size: 5171
BanI G'GyrC_C
Cuts at: 2187 2601 2849 2884 2187
Size: 414 248 35 4474
Fragments arranged by size:
4474 414 248 35
BanII G_rGCy'C
Cuts at: 404 1811 1855 2185 3215 404
Size: 1407 44 330 1030 2360
Fragments arranged by size:
2360 1407 1030 330 44
BbeI G_GCGC'C
Cuts at: 2605 2853 2605
Size: 248 4923
BbsI GAAGACnn'nnnn_
Cuts at: 1895 2088 1895
Size: 193 4978
BciVI GTATCCnnnnn_n'
Cuts at: 3069 3830 5171 3069
Size: 761 1341 3069
Fragments arranged by size:
3069 1341 761
BclI T'GATC_A
Cuts at: 2691 2691
Size: 5171
BglII A'GATC_T
Cuts at: 2686 4723 2686
Size: 2037 3134
BlpI GC'TnA_GC
Cuts at: 2283 2283
Size: 5171
Bme1580I G_kGCm'C
Cuts at: 1006 1535 2796 2889 3945 1006
Size: 529 1261 93 1056 2232
Fragments arranged by size:
2232 1261 1056 529 93
BmgBI CAC'GTC
Cuts at: 735 1925 735
Size: 1190 3981
BmrI ACTGGGnnnn_n'
Cuts at: 868 990 1707 2383 2464 2799 4943 868
Size: 122 717 676 81 335 2144 1096
Fragments arranged by size:
2144 1096 717 676 335 122 81
BmtI G_CTAG'C
Cuts at: 1736 1736
Size: 5171
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3538 3538
Size: 5171
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1531 1656 1710 2008 1531
Size: 125 54 298 4694
Fragments arranged by size:
4694 298 125 54
Bpu10I CC'TnA_GC
Cuts at: 972 1802 1916 972
Size: 830 114 4227
Fragments arranged by size:
4227 830 114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 524 2347 3226 3718 4016 524
Size: 1823 879 492 298 1679
Fragments arranged by size:
1823 1679 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4875 4875
Size: 5171
BsaAI yAC'GTr
Cuts at: 422 521 638 1224 2163 2524 422
Size: 99 117 586 939 361 3069
Fragments arranged by size:
3069 939 586 361 117 99
BsaBI GATnn'nnATC
Cuts at: 6 2685 4722 6
Size: 2679 2037 455
Fragments arranged by size:
2679 2037 455
BsaHI Gr'CG_yC
Cuts at: 1307 2602 2850 3552 5048 1307
Size: 1295 248 702 1496 1430
Fragments arranged by size:
1496 1430 1295 702 248
BsaWI w'CCGG_w
Cuts at: 2360 2881 3559 3833 3980 4391 2360
Size: 521 678 274 147 411 3140
Fragments arranged by size:
3140 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1733 1763 1733
Size: 30 5141
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 296 296
Size: 5171
BseYI C'CCAG_C
Cuts at: 1030 1049 1172 1858 2596 3354 3931 4427 1030
Size: 19 123 686 738 758 577 496 1774
Fragments arranged by size:
1774 758 738 686 577 496 123 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 252 898 1168 1429 1434 2012 252
Size: 646 270 261 5 578 3411
Fragments arranged by size:
3411 646 578 270 261 5
BsiEI CG_ry'CG
Cuts at: 104 495 686 2713 2759 3967 104
Size: 391 191 2027 46 1208 1308
Fragments arranged by size:
2027 1308 1208 391 191 46
BsiHKAI G_wGCw'C
Cuts at: 1006 1124 1241 1811 2185 2963 3153 3945 1006
Size: 118 117 570 374 778 190 792 2232
Fragments arranged by size:
2232 792 778 570 374 190 118 117
BsmI GAATG_Cn'
Cuts at: 728 728
Size: 5171
BsmBI CGTCTCn'nnnn_
Cuts at: 231 231
Size: 5171
Bsp1286I G_dGCh'C
Cuts at: 404 1006 1124 1241 1535 1811 1855 2185 2796
Size: 602 118 117 294 276 44 330 611
Cuts at: 2796 2889 2963 3153 3215 3945 404
Size: 93 74 190 62 730 1630
Fragments arranged by size:
1630 730 611 602 330 294 276 190
118 117 93 74 62 44
BspEI T'CCGG_A
Cuts at: 4391 4391
Size: 5171
BspHI T'CATG_A
Cuts at: 4347 4347
Size: 5171
BspMI ACCTGCnnnn'nnnn_
Cuts at: 458 495 684 2221 2435 2737 3118 458
Size: 37 189 1537 214 302 381 2511
Fragments arranged by size:
2511 1537 381 302 214 189 37
BsrBI CCG'CTC
Cuts at: 50 971 1440 3463 3517 5001 5166 50
Size: 921 469 2023 54 1484 165 55
Fragments arranged by size:
2023 1484 921 469 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3083 3083
Size: 5171
BsrFI r'CCGG_y
Cuts at: 1429 3169 3350 3559 1429
Size: 1740 181 209 3041
Fragments arranged by size:
3041 1740 209 181
BssSI C'ACGA_G
Cuts at: 745 1144 3442 3800 745
Size: 399 2298 358 2116
Fragments arranged by size:
2298 2116 399 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2548 2548
Size: 5171
BstBI TT'CG_AA
Cuts at: 110 3532 110
Size: 3422 1749
BstEII G'GTnAC_C
Cuts at: 1177 1177
Size: 5171
BstYI r'GATC_y
Cuts at: 1 1389 2196 2663 2686 3021 3267 4279 4365
Size: 1388 807 467 23 335 246 1012 86
Cuts at: 4365 4377 4723 1
Size: 12 346 449
Fragments arranged by size:
1388 1012 807 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 266 495 714 959 1091 1591 1822 2029 2072
Size: 229 219 245 132 500 231 207 43
Cuts at: 2072 266
Size: 3365
Fragments arranged by size:
3365 500 245 231 229 219 207 132
43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 440 1263 3121 3302 3465 440
Size: 823 1858 181 163 2146
Fragments arranged by size:
2146 1858 823 181 163
BtsI GCAGTG_nn'
Cuts at: 143 168 1352 1446 2414 143
Size: 25 1184 94 968 2900
Fragments arranged by size:
2900 1184 968 94 25
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1095 1130 1095
Size: 35 5136
DraI TTT'AAA
Cuts at: 2170 2170
Size: 5171
DraIII CAC_nnn'GTG
Cuts at: 1245 1245
Size: 5171
DrdI GACnn_nn'nnGTC
Cuts at: 1306 2877 3735 1306
Size: 1571 858 2742
Fragments arranged by size:
2742 1571 858
EaeI y'GGCC_r
Cuts at: 263 416 683 1567 2756 2930 3321 3348 4693
Size: 153 267 884 1189 174 391 27 1345
Cuts at: 4693 263
Size: 741
Fragments arranged by size:
1345 1189 884 741 391 267 174 153
27
EagI C'GGCC_G
Cuts at: 683 2756 683
Size: 2073 3098
EarI CTCTTCn'nnn_
Cuts at: 1078 3194 3404 1078
Size: 2116 210 2845
Fragments arranged by size:
2845 2116 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3379 3689 3835 4981 3379
Size: 310 146 1146 3569
Fragments arranged by size:
3569 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1809 2183 1809
Size: 374 4797
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1102 1531 1656 1710 2008 2996 3428 4175 4443
Size: 429 125 54 298 988 432 747 268
Cuts at: 4443 1102
Size: 1830
Fragments arranged by size:
1830 988 747 432 429 298 268 125
54
EcoNI CCTnn'n_nnAGG
Cuts at: 796 796
Size: 5171
EcoO109I rG'GnC_Cy
Cuts at: 2310 2310
Size: 5171
EcoRI G'AATT_C
Cuts at: 2175 2175
Size: 5171
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3190 3190
Size: 5171
FspI TGC'GCA
Cuts at: 1837 2952 1837
Size: 1115 4056
HaeII r_GCGC'y
Cuts at: 1155 2605 2853 3875 4854 1155
Size: 1450 248 1022 979 1472
Fragments arranged by size:
1472 1450 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 808 840 937 969 1397 1429 2031 2063 3013
Size: 32 97 32 428 32 602 32 950
Cuts at: 3013 3043 3045 3075 808
Size: 30 2 30 2904
Fragments arranged by size:
2904 950 602 428 97 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 489 2210 489
Size: 1721 3450
KasI G'GCGC_C
Cuts at: 2601 2849 2601
Size: 248 4923
KpnI G_GTAC'C
Cuts at: 2191 2191
Size: 5171
MluI A'CGCG_T
Cuts at: 4604 4604
Size: 5171
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 194 3842 4026 194
Size: 3648 184 1339
Fragments arranged by size:
3648 1339 184
MscI TGG'CCA
Cuts at: 265 418 2932 265
Size: 153 2514 2504
Fragments arranged by size:
2514 2504 153
MslI CAynn'nnrTG
Cuts at: 445 874 1144 3160 3287 445
Size: 429 270 2016 127 2329
Fragments arranged by size:
2329 2016 429 270 127
MspA1I CmG'CkG
Cuts at: 497 1260 1593 1655 2031 2282 2596 2956 3969
Size: 763 333 62 376 251 314 360 1013
Cuts at: 3969 4214 497
Size: 245 1454
Fragments arranged by size:
1454 1013 763 376 360 333 314 251
245 62
NaeI GCC'GGC
Cuts at: 1431 3352 1431
Size: 1921 3250
NarI GG'CG_CC
Cuts at: 2602 2850 2602
Size: 248 4923
NcoI C'CATG_G
Cuts at: 1091 2072 1091
Size: 981 4190
NdeI CA'TA_TG
Cuts at: 646 646
Size: 5171
NgoMIV G'CCGG_C
Cuts at: 1429 3350 1429
Size: 1921 3250
NheI G'CTAG_C
Cuts at: 1732 1732
Size: 5171
NspI r_CATG'y
Cuts at: 771 2224 2724 3631 771
Size: 1453 500 907 2311
Fragments arranged by size:
2311 1453 907 500
PciI A'CATG_T
Cuts at: 3627 3627
Size: 5171
PflMI CCAn_nnn'nTGG
Cuts at: 414 1172 414
Size: 758 4413
PfoI T'CCnGG_A
Cuts at: 187 772 187
Size: 585 4586
PmeI GTTT'AAAC
Cuts at: 2170 2170
Size: 5171
PmlI CAC'GTG
Cuts at: 422 521 422
Size: 99 5072
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4310 4342 4310
Size: 32 5139
PshAI GACnn'nnGTC
Cuts at: 603 603
Size: 5171
PstI C_TGCA'G
Cuts at: 1259 2218 2903 1259
Size: 959 685 3527
Fragments arranged by size:
3527 959 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5171
PvuII CAG'CTG
Cuts at: 1260 1655 2596 2956 1260
Size: 395 941 360 3475
Fragments arranged by size:
3475 941 395 360
RsrII CG'GwC_CG
Cuts at: 3366 3366
Size: 5171
SacI G_AGCT'C
Cuts at: 1811 2185 1811
Size: 374 4797
SacII CC_GC'GG
Cuts at: 498 1594 2032 498
Size: 1096 438 3637
Fragments arranged by size:
3637 1096 438
SalI G'TCGA_C
Cuts at: 2208 2208
Size: 5171
SapI GCTCTTCn'nnn_
Cuts at: 3194 3404 3194
Size: 210 4961
SbfI CC_TGCA'GG
Cuts at: 2218 2218
Size: 5171
ScaI AGT'ACT
Cuts at: 333 4688 333
Size: 4355 816
SexAI A'CCwGG_T
Cuts at: 2136 2136
Size: 5171
SfcI C'TryA_G
Cuts at: 33 1255 2214 2899 3892 4083 33
Size: 1222 959 685 993 191 1121
Fragments arranged by size:
1222 1121 993 959 685 191
SfoI GGC'GCC
Cuts at: 2603 2851 2603
Size: 248 4923
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5171
SmaI CCC'GGG
Cuts at: 1795 2193 1795
Size: 398 4773
SmlI C'TyrA_G
Cuts at: 539 2326 3241 3733 3995 539
Size: 1787 915 492 262 1715
Fragments arranged by size:
1787 1715 915 492 262
SnaBI TAC'GTA
Cuts at: 2163 2163
Size: 5171
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5171
SphI G_CATG'C
Cuts at: 2224 2724 2224
Size: 500 4671
StyI C'CwwG_G
Cuts at: 1091 1753 2072 2305 2659 1091
Size: 662 319 233 354 3603
Fragments arranged by size:
3603 662 354 319 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1758 1797 2754 2887 3346 3555 1758
Size: 39 957 133 459 209 3374
Fragments arranged by size:
3374 957 459 209 133 39
TatI w'GTAC_w
Cuts at: 331 4686 331
Size: 4355 816
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 183 215 592 624 1231 1263 1733 1765 4688
Size: 32 377 32 607 32 470 32 2923
Cuts at: 4688 4720 183
Size: 32 634
Fragments arranged by size:
2923 634 607 470 377 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 757 1411 2968 757
Size: 654 1557 2960
Fragments arranged by size:
2960 1557 654
XbaI T'CTAG_A
Cuts at: 2202 2202
Size: 5171
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2666 2666
Size: 5171
XmaI C'CCGG_G
Cuts at: 1793 2191 1793
Size: 398 4773
ZraI GAC'GTC
Cuts at: 1308 1308
Size: 5171
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NspI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BsiWI BsrGI BssHII
BstXI BstZ17I Bsu36I ClaI EcoRV FseI FspAI HindIII
HpaI MfeI NotI NruI NsiI PacI PasI PpuMI
PsiI PspOMI PspXI PsrI SanDI SfiI SgrAI SrfI
SspI StuI SwaI XhoI XmnI