Restriction map for
pF1KSDA1400
(Circular) (Six-base) MAPSORT of: pF1KSDA1400.seq Check: 2328 from: 1 to: 6257
pF1KSDA1400 6257 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1290 1290
Size: 6257
AccI GT'mk_AC
Cuts at: 887 1526 3295 887
Size: 639 1769 3849
Fragments arranged by size:
3849 1769 639
Acc65I G'GTAC_C
Cuts at: 3273 3273
Size: 6257
AclI AA'CG_TT
Cuts at: 6097 6097
Size: 6257
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 975 1188 1725 1753 4082 4514 5261 5529
Size: 715 213 537 28 2329 432 747 268
Cuts at: 5529 260
Size: 988
Fragments arranged by size:
2329 988 747 715 537 432 268 213
28
AflII C'TTAA_G
Cuts at: 2635 2635
Size: 6257
AflIII A'CryG_T
Cuts at: 1532 3609 4713 5690 1532
Size: 2077 1104 977 2099
Fragments arranged by size:
2099 2077 1104 977
AgeI A'CCGG_T
Cuts at: 2698 4645 2698
Size: 1947 4310
AleI CACnn'nnGTG
Cuts at: 223 928 1252 1930 2146 3079 223
Size: 705 324 678 216 933 3401
Fragments arranged by size:
3401 933 705 678 324 216
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3058 3058
Size: 6257
AlwNI CAG_nnn'CTG
Cuts at: 1785 3076 3562 5129 1785
Size: 1291 486 1567 2913
Fragments arranged by size:
2913 1567 1291 486
ApaI G_GGCC'C
Cuts at: 1129 1129
Size: 6257
ApaLI G'TGCA_C
Cuts at: 712 5027 712
Size: 4315 1942
ApoI r'AATT_y
Cuts at: 3261 5610 5866 3261
Size: 2349 256 3652
Fragments arranged by size:
3652 2349 256
AseI AT'TA_AT
Cuts at: 20 470 20
Size: 450 5807
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6257
AvaI C'yCGr_G
Cuts at: 1010 1430 1633 1760 2450 3277 1010
Size: 420 203 127 690 827 3990
Fragments arranged by size:
3990 827 690 420 203 127
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1468 1501 1468
Size: 33 6224
BamHI G'GATC_C
Cuts at: 3282 3282
Size: 6257
BanI G'GyrC_C
Cuts at: 291 580 1239 1561 1603 1851 2167 2529 3273
Size: 289 659 322 42 248 316 362 744
Cuts at: 3273 3687 3935 3970 291
Size: 414 248 35 2578
Fragments arranged by size:
2578 744 659 414 362 322 316 289
248 248 42 35
BanII G_rGCy'C
Cuts at: 391 1090 1129 2180 2296 2503 2780 3271 4301
Size: 699 39 1051 116 207 277 491 1030
Cuts at: 4301 391
Size: 2347
Fragments arranged by size:
2347 1051 1030 699 491 277 207 116
39
BbeI G_GCGC'C
Cuts at: 1243 1565 1607 1855 2171 3691 3939 1243
Size: 322 42 248 316 1520 248 3561
Fragments arranged by size:
3561 1520 322 316 248 248 42
BbsI GAAGACnn'nnnn_
Cuts at: 1337 1653 1337
Size: 316 5941
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 645 679 645
Size: 34 6223
BciVI GTATCCnnnnn_n'
Cuts at: 4155 4916 6257 4155
Size: 761 1341 4155
Fragments arranged by size:
4155 1341 761
BclI T'GATC_A
Cuts at: 2889 3777 2889
Size: 888 5369
BglI GCCn_nnn'nGGC
Cuts at: 1849 1849
Size: 6257
BglII A'GATC_T
Cuts at: 243 3772 5809 243
Size: 3529 2037 691
Fragments arranged by size:
3529 2037 691
BlpI GC'TnA_GC
Cuts at: 668 2779 3369 668
Size: 2111 590 3556
Fragments arranged by size:
3556 2111 590
Bme1580I G_kGCm'C
Cuts at: 296 583 716 869 1129 2913 3882 3975 5031
Size: 287 133 153 260 1784 969 93 1056
Cuts at: 5031 296
Size: 1522
Fragments arranged by size:
1784 1522 1056 969 287 260 153 133
93
BmgBI CAC'GTC
Cuts at: 576 636 1326 1533 576
Size: 60 690 207 5300
Fragments arranged by size:
5300 690 207 60
BmrI ACTGGGnnnn_n'
Cuts at: 1084 1327 2225 2507 2907 3469 3550 3885 6029
Size: 243 898 282 400 562 81 335 2144
Cuts at: 6029 1084
Size: 1312
Fragments arranged by size:
2144 1312 898 562 400 335 282 243
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2794 4624 2794
Size: 1830 4427
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 430 442 454 700 1075 1862 3077 3184
Size: 81 12 12 246 375 787 1215 107
Cuts at: 3184 349
Size: 3422
Fragments arranged by size:
3422 1215 787 375 246 107 81 12
12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 285 1324 3433 4312 4804 5102 285
Size: 1039 2109 879 492 298 1440
Fragments arranged by size:
2109 1440 1039 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5961 5961
Size: 6257
BsaAI yAC'GTr
Cuts at: 348 723 1107 3249 3610 348
Size: 375 384 2142 361 2995
Fragments arranged by size:
2995 2142 384 375 361
BsaBI GATnn'nnATC
Cuts at: 6 2888 3771 5808 6
Size: 2882 883 2037 455
Fragments arranged by size:
2882 2037 883 455
BsaHI Gr'CG_yC
Cuts at: 1240 1562 1604 1852 2168 3015 3688 3936 4638
Size: 322 42 248 316 847 673 248 702
Cuts at: 4638 6134 1240
Size: 1496 1363
Fragments arranged by size:
1496 1363 847 702 673 322 316 248
248 42
BsaWI w'CCGG_w
Cuts at: 2698 3446 3967 4645 4919 5066 5477 2698
Size: 748 521 678 274 147 411 3478
Fragments arranged by size:
3478 748 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 217 758 770 1303 2554 217
Size: 541 12 533 1251 3920
Fragments arranged by size:
3920 1251 541 533 12
BseYI C'CCAG_C
Cuts at: 179 803 1613 2075 3101 3682 4440 5017 5513
Size: 624 810 462 1026 581 758 577 496
Cuts at: 5513 179
Size: 923
Fragments arranged by size:
1026 923 810 758 624 581 577 496
462
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 658 1324 2515 388
Size: 270 666 1191 4130
Fragments arranged by size:
4130 1191 666 270
BsiEI CG_ry'CG
Cuts at: 104 1593 3799 3845 5053 104
Size: 1489 2206 46 1208 1308
Fragments arranged by size:
2206 1489 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 716 2222 2503 2675 2780 3271 4049 4239 5031
Size: 1506 281 172 105 491 778 190 792
Cuts at: 5031 716
Size: 1942
Fragments arranged by size:
1942 1506 792 778 491 281 190 172
105
BsmI GAATG_Cn'
Cuts at: 562 2821 562
Size: 2259 3998
BsmBI CGTCTCn'nnnn_
Cuts at: 1316 1316
Size: 6257
Bsp1286I G_dGCh'C
Cuts at: 296 391 583 716 869 1090 1129 2180 2222
Size: 95 192 133 153 221 39 1051 42
Cuts at: 2222 2296 2503 2675 2780 2913 3271 3882 3975
Size: 74 207 172 105 133 358 611 93
Cuts at: 3975 4049 4239 4301 5031 296
Size: 74 190 62 730 1522
Fragments arranged by size:
1522 1051 730 611 358 221 207 192
190 172 153 133 133 105 95 93
74 74 62 42 39
BspEI T'CCGG_A
Cuts at: 5477 5477
Size: 6257
BspHI T'CATG_A
Cuts at: 2892 5433 2892
Size: 2541 3716
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1290 1928 2440 3307 3521 3823 4204 1290
Size: 638 512 867 214 302 381 3343
Fragments arranged by size:
3343 867 638 512 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1901 2198 3117 4549 4603 6087 6252 50
Size: 1851 297 919 1432 54 1484 165 55
Fragments arranged by size:
1851 1484 1432 919 297 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4169 4169
Size: 6257
BsrFI r'CCGG_y
Cuts at: 817 1521 2698 4255 4436 4645 817
Size: 704 1177 1557 181 209 2429
Fragments arranged by size:
2429 1557 1177 704 209 181
BssHII G'CGCG_C
Cuts at: 2455 2455
Size: 6257
BssSI C'ACGA_G
Cuts at: 928 4528 4886 928
Size: 3600 358 2299
Fragments arranged by size:
3600 2299 358
BstAPI GCAn_nnn'nTGC
Cuts at: 711 3634 711
Size: 2923 3334
BstBI TT'CG_AA
Cuts at: 110 4618 110
Size: 4508 1749
BstEII G'GTnAC_C
Cuts at: 1915 1915
Size: 6257
BstXI CCAn_nnnn'nTGG
Cuts at: 652 1122 2148 2920 652
Size: 470 1026 772 3989
Fragments arranged by size:
3989 1026 772 470
BstYI r'GATC_y
Cuts at: 1 243 1643 2928 3282 3749 3772 4107 4353
Size: 242 1400 1285 354 467 23 335 246
Cuts at: 4353 5365 5451 5463 5809 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1400 1285 1012 467 449 354 346 335
246 242 86 23 12
BtgI C'CryG_G
Cuts at: 731 1940 2112 2171 731
Size: 1209 172 59 4817
Fragments arranged by size:
4817 1209 172 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2268 4207 4388 4551 2268
Size: 1939 181 163 3974
Fragments arranged by size:
3974 1939 181 163
BtsI GCAGTG_nn'
Cuts at: 1146 1312 3500 1146
Size: 166 2188 3903
Fragments arranged by size:
3903 2188 166
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3206 3241 3206
Size: 35 6222
DraI TTT'AAA
Cuts at: 3256 3256
Size: 6257
DrdI GACnn_nn'nnGTC
Cuts at: 883 2607 3963 4821 883
Size: 1724 1356 858 2319
Fragments arranged by size:
2319 1724 1356 858
EaeI y'GGCC_r
Cuts at: 1934 2060 2318 2333 2381 2439 3842 4016 4407
Size: 126 258 15 48 58 1403 174 391
Cuts at: 4407 4434 5779 1934
Size: 27 1345 2412
Fragments arranged by size:
2412 1403 1345 391 258 174 126 58
48 27 15
EagI C'GGCC_G
Cuts at: 3842 3842
Size: 6257
EarI CTCTTCn'nnn_
Cuts at: 1076 2938 4280 4490 1076
Size: 1862 1342 210 2843
Fragments arranged by size:
2843 1862 1342 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1324 1921 2219 2435 2601 4465 4775 4921 6067
Size: 597 298 216 166 1864 310 146 1146
Cuts at: 6067 1324
Size: 1514
Fragments arranged by size:
1864 1514 1146 597 310 298 216 166
146
EcoICRI GAG'CTC
Cuts at: 2501 2778 3269 2501
Size: 277 491 5489
Fragments arranged by size:
5489 491 277
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 349 430 442 454 700 975 1075 1188
Size: 89 81 12 12 246 275 100 113
Cuts at: 1188 1725 1753 1862 3077 3184 4082 4514 5261
Size: 537 28 109 1215 107 898 432 747
Cuts at: 5261 5529 260
Size: 268 988
Fragments arranged by size:
1215 988 898 747 537 432 275 268
246 113 109 107 100 89 81 28
12 12
EcoNI CCTnn'n_nnAGG
Cuts at: 1859 1859
Size: 6257
EcoO109I rG'GnC_Cy
Cuts at: 305 1119 1126 3147 3396 305
Size: 814 7 2021 249 3166
Fragments arranged by size:
3166 2021 814 249 7
EcoRI G'AATT_C
Cuts at: 3261 3261
Size: 6257
EcoRV GAT'ATC
Cuts at: 2724 2724
Size: 6257
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 326 1365 4276 326
Size: 1039 2911 2307
Fragments arranged by size:
2911 2307 1039
FspI TGC'GCA
Cuts at: 1145 2041 4038 1145
Size: 896 1997 3364
Fragments arranged by size:
3364 1997 896
HaeII r_GCGC'y
Cuts at: 530 1243 1565 1586 1607 1786 1855 2171 2229
Size: 713 322 21 21 179 69 316 58
Cuts at: 2229 3208 3691 3939 4961 5940 530
Size: 979 483 248 1022 979 847
Fragments arranged by size:
1022 979 979 847 713 483 322 316
248 179 69 58 21 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1267 1299 2713 2745 2920 2952 4099 4129 4131
Size: 32 1414 32 175 32 1147 30 2
Cuts at: 4131 4161 1267
Size: 30 3363
Fragments arranged by size:
3363 1414 1147 175 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1806 2793 2811 3296 1806
Size: 987 18 485 4767
Fragments arranged by size:
4767 987 485 18
HpaI GTT'AAC
Cuts at: 2811 2811
Size: 6257
KasI G'GCGC_C
Cuts at: 1239 1561 1603 1851 2167 3687 3935 1239
Size: 322 42 248 316 1520 248 3561
Fragments arranged by size:
3561 1520 322 316 248 248 42
KpnI G_GTAC'C
Cuts at: 3277 3277
Size: 6257
MfeI C'AATT_G
Cuts at: 2940 2940
Size: 6257
MluI A'CGCG_T
Cuts at: 5690 5690
Size: 6257
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1052 1466 2756 2774 4928 5112 1052
Size: 414 1290 18 2154 184 2197
Fragments arranged by size:
2197 2154 1290 414 184 18
MscI TGG'CCA
Cuts at: 2320 2383 4018 2320
Size: 63 1635 4559
Fragments arranged by size:
4559 1635 63
MslI CAynn'nnrTG
Cuts at: 130 223 841 928 1252 1930 2146 2326 2491
Size: 93 618 87 324 678 216 180 165
Cuts at: 2491 3079 4246 4373 130
Size: 588 1167 127 2014
Fragments arranged by size:
2014 1167 678 618 588 324 216 180
165 127 93 87
MspA1I CmG'CkG
Cuts at: 733 1731 2079 2157 2920 3368 3682 4042 5055
Size: 998 348 78 763 448 314 360 1013
Cuts at: 5055 5300 733
Size: 245 1690
Fragments arranged by size:
1690 1013 998 763 448 360 348 314
245 78
NaeI GCC'GGC
Cuts at: 4438 4438
Size: 6257
NarI GG'CG_CC
Cuts at: 1240 1562 1604 1852 2168 3688 3936 1240
Size: 322 42 248 316 1520 248 3561
Fragments arranged by size:
3561 1520 322 316 248 248 42
NcoI C'CATG_G
Cuts at: 2112 2112
Size: 6257
NgoMIV G'CCGG_C
Cuts at: 4436 4436
Size: 6257
NspI r_CATG'y
Cuts at: 3049 3310 3810 4717 3049
Size: 261 500 907 4589
Fragments arranged by size:
4589 907 500 261
PasI CC'CwG_GG
Cuts at: 645 2181 645
Size: 1536 4721
PciI A'CATG_T
Cuts at: 4713 4713
Size: 6257
PflMI CCAn_nnn'nTGG
Cuts at: 1785 1785
Size: 6257
PfoI T'CCnGG_A
Cuts at: 491 2825 491
Size: 2334 3923
PmeI GTTT'AAAC
Cuts at: 3256 3256
Size: 6257
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5396 5428 5396
Size: 32 6225
PpuMI rG'GwC_Cy
Cuts at: 305 1119 305
Size: 814 5443
PshAI GACnn'nnGTC
Cuts at: 2858 2858
Size: 6257
PspOMI G'GGCC_C
Cuts at: 1125 1125
Size: 6257
PspXI vC'TCGA_Gb
Cuts at: 1633 1633
Size: 6257
PstI C_TGCA'G
Cuts at: 2647 3304 3989 2647
Size: 657 685 4915
Fragments arranged by size:
4915 685 657
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6257
PvuII CAG'CTG
Cuts at: 1731 2157 2920 3682 4042 1731
Size: 426 763 762 360 3946
Fragments arranged by size:
3946 763 762 426 360
RsrII CG'GwC_CG
Cuts at: 4452 4452
Size: 6257
SacI G_AGCT'C
Cuts at: 2503 2780 3271 2503
Size: 277 491 5489
Fragments arranged by size:
5489 491 277
SacII CC_GC'GG
Cuts at: 734 734
Size: 6257
SalI G'TCGA_C
Cuts at: 3294 3294
Size: 6257
SapI GCTCTTCn'nnn_
Cuts at: 4280 4490 4280
Size: 210 6047
SbfI CC_TGCA'GG
Cuts at: 3304 3304
Size: 6257
ScaI AGT'ACT
Cuts at: 1169 5774 1169
Size: 4605 1652
SfcI C'TryA_G
Cuts at: 33 1421 2643 3300 3985 4978 5169 33
Size: 1388 1222 657 685 993 191 1121
Fragments arranged by size:
1388 1222 1121 993 685 657 191
SfoI GGC'GCC
Cuts at: 1241 1563 1605 1853 2169 3689 3937 1241
Size: 322 42 248 316 1520 248 3561
Fragments arranged by size:
3561 1520 322 316 248 248 42
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6257
SmaI CCC'GGG
Cuts at: 1012 1762 2452 3279 1012
Size: 750 690 827 3990
Fragments arranged by size:
3990 827 750 690
SmlI C'TyrA_G
Cuts at: 300 1339 1633 2635 3412 4327 4819 5081 300
Size: 1039 294 1002 777 915 492 262 1476
Fragments arranged by size:
1476 1039 1002 915 777 492 294 262
SnaBI TAC'GTA
Cuts at: 3249 3249
Size: 6257
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6257
SphI G_CATG'C
Cuts at: 3310 3810 3310
Size: 500 5757
SrfI GCCC'GGGC
Cuts at: 2452 2452
Size: 6257
StuI AGG'CCT
Cuts at: 794 794
Size: 6257
StyI C'CwwG_G
Cuts at: 551 1115 2112 3391 3745 551
Size: 564 997 1279 354 3063
Fragments arranged by size:
3063 1279 997 564 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 710 1406 2635 3840 3973 4432 4641 710
Size: 696 1229 1205 133 459 209 2326
Fragments arranged by size:
2326 1229 1205 696 459 209 133
TatI w'GTAC_w
Cuts at: 983 1167 5772 983
Size: 184 4605 1468
Fragments arranged by size:
4605 1468 184
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2039 2071 5774 5806 2039
Size: 32 3703 32 2490
Fragments arranged by size:
3703 2490 32 32
Tth111I GACn'n_nGTC
Cuts at: 4054 4054
Size: 6257
XbaI T'CTAG_A
Cuts at: 3288 3288
Size: 6257
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 843 1786 2142 2328 3752 843
Size: 943 356 186 1424 3348
Fragments arranged by size:
3348 1424 943 356 186
XhoI C'TCGA_G
Cuts at: 1633 1633
Size: 6257
XmaI C'CCGG_G
Cuts at: 1010 1760 2450 3277 1010
Size: 750 690 827 3990
Fragments arranged by size:
3990 827 750 690
XmnI GAAnn'nnTTC
Cuts at: 1738 1738
Size: 6257
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AloI AscI AvrII BbvCI BfrBI
BmtI Bpu10I BsaXI BsiWI BsrGI BstZ17I Bsu36I ClaI
DraIII FseI FspAI HindIII NdeI NheI NotI NruI
NsiI PacI PmlI PsiI PsrI SanDI SexAI SfiI
SgrAI SspI SwaI ZraI