Restriction map for
pF1KSDA1412
(Circular) (Six-base) MAPSORT of: pF1KSDA1412.seq Check: 6093 from: 1 to: 7286
pF1KSDA1412 7286 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3711 3711
Size: 7286
AccI GT'mk_AC
Cuts at: 1562 4324 1562
Size: 2762 4524
Acc65I G'GTAC_C
Cuts at: 4302 4302
Size: 7286
AclI AA'CG_TT
Cuts at: 7126 7126
Size: 7286
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 338 1000 1691 5111 5543 6290 6558 245
Size: 93 662 691 3420 432 747 268 973
Fragments arranged by size:
3420 973 747 691 662 432 268 93
AflIII A'CryG_T
Cuts at: 2430 2892 4638 5742 6719 2430
Size: 462 1746 1104 977 2997
Fragments arranged by size:
2997 1746 1104 977 462
AgeI A'CCGG_T
Cuts at: 5674 5674
Size: 7286
AhdI GACnn_n'nnGTC
Cuts at: 1839 1839
Size: 7286
AleI CACnn'nnGTG
Cuts at: 1541 1784 1541
Size: 243 7043
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 720 1891 3105 720
Size: 1171 1214 4901
Fragments arranged by size:
4901 1214 1171
AlwNI CAG_nnn'CTG
Cuts at: 1533 3554 3620 4591 6158 1533
Size: 2021 66 971 1567 2661
Fragments arranged by size:
2661 2021 1567 971 66
ApaI G_GGCC'C
Cuts at: 2756 2756
Size: 7286
ApaLI G'TGCA_C
Cuts at: 6056 6056
Size: 7286
ApoI r'AATT_y
Cuts at: 268 867 959 1270 1380 1583 1698 2295 2517
Size: 599 92 311 110 203 115 597 222
Cuts at: 2517 2838 3478 4290 6639 6895 268
Size: 321 640 812 2349 256 659
Fragments arranged by size:
2349 812 659 640 599 597 321 311
256 222 203 115 110 92
AseI AT'TA_AT
Cuts at: 20 2150 3200 20
Size: 2130 1050 4106
Fragments arranged by size:
4106 2130 1050
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7286
AvaI C'yCGr_G
Cuts at: 917 4306 917
Size: 3389 3897
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2799 2832 2799
Size: 33 7253
BamHI G'GATC_C
Cuts at: 495 628 1038 3876 4311 495
Size: 133 410 2838 435 3470
Fragments arranged by size:
3470 2838 435 410 133
BanI G'GyrC_C
Cuts at: 3279 4302 4716 4964 4999 3279
Size: 1023 414 248 35 5566
Fragments arranged by size:
5566 1023 414 248 35
BanII G_rGCy'C
Cuts at: 2756 4300 5330 2756
Size: 1544 1030 4712
Fragments arranged by size:
4712 1544 1030
BbeI G_GCGC'C
Cuts at: 4720 4968 4720
Size: 248 7038
BbsI GAAGACnn'nnnn_
Cuts at: 296 296
Size: 7286
BbvCI CC'TCA_GC
Cuts at: 1574 1574
Size: 7286
BciVI GTATCCnnnnn_n'
Cuts at: 1804 2995 3179 5184 5945 7286 1804
Size: 1191 184 2005 761 1341 1804
Fragments arranged by size:
2005 1804 1341 1191 761 184
BclI T'GATC_A
Cuts at: 4806 4806
Size: 7286
BglII A'GATC_T
Cuts at: 4801 6838 4801
Size: 2037 5249
BlpI GC'TnA_GC
Cuts at: 4398 4398
Size: 7286
Bme1580I G_kGCm'C
Cuts at: 831 2756 4911 5004 6060 831
Size: 1925 2155 93 1056 2057
Fragments arranged by size:
2155 2057 1925 1056 93
BmgBI CAC'GTC
Cuts at: 1794 1794
Size: 7286
BmrI ACTGGGnnnn_n'
Cuts at: 1159 2222 4498 4579 4914 7058 1159
Size: 1063 2276 81 335 2144 1387
Fragments arranged by size:
2276 2144 1387 1063 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 562 5653 562
Size: 5091 2195
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 434 835 1433 2696 2783 3265 3477 434
Size: 401 598 1263 87 482 212 4243
Fragments arranged by size:
4243 1263 598 482 401 212 87
Bpu10I CC'TnA_GC
Cuts at: 1574 1574
Size: 7286
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1845 4462 5341 5833 6131 1845
Size: 2617 879 492 298 3000
Fragments arranged by size:
3000 2617 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1409 3797 4232 6990 1409
Size: 2388 435 2758 1705
Fragments arranged by size:
2758 2388 1705 435
BsaAI yAC'GTr
Cuts at: 2431 4278 4639 2431
Size: 1847 361 5078
Fragments arranged by size:
5078 1847 361
BsaBI GATnn'nnATC
Cuts at: 6 473 3167 4800 6837 6
Size: 467 2694 1633 2037 455
Fragments arranged by size:
2694 2037 1633 467 455
BsaHI Gr'CG_yC
Cuts at: 563 3857 4717 4965 5667 7163 563
Size: 3294 860 248 702 1496 686
Fragments arranged by size:
3294 1496 860 702 686 248
BsaWI w'CCGG_w
Cuts at: 4475 4996 5674 5948 6095 6506 4475
Size: 521 678 274 147 411 5255
Fragments arranged by size:
5255 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2474 2504 2474
Size: 30 7256
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 230 233 1477 1984 2510 2513 3853 4016 230
Size: 3 1244 507 526 3 1340 163 3500
Fragments arranged by size:
3500 1340 1244 526 507 163 3 3
BseYI C'CCAG_C
Cuts at: 3794 4711 5469 6046 6542 3794
Size: 917 758 577 496 4538
Fragments arranged by size:
4538 917 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1519 3872 3903 1519
Size: 2353 31 4902
Fragments arranged by size:
4902 2353 31
BsiEI CG_ry'CG
Cuts at: 104 4828 4874 6082 104
Size: 4724 46 1208 1308
Fragments arranged by size:
4724 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2776 3133 3191 3997 4300 5078 5268 6060 2776
Size: 357 58 806 303 778 190 792 4002
Fragments arranged by size:
4002 806 792 778 357 303 190 58
BsmI GAATG_Cn'
Cuts at: 307 573 1495 3084 3438 307
Size: 266 922 1589 354 4155
Fragments arranged by size:
4155 1589 922 354 266
BsmBI CGTCTCn'nnnn_
Cuts at: 487 487
Size: 7286
Bsp1286I G_dGCh'C
Cuts at: 831 2756 2776 3133 3191 3997 4300 4911 5004
Size: 1925 20 357 58 806 303 611 93
Cuts at: 5004 5078 5268 5330 6060 831
Size: 74 190 62 730 2057
Fragments arranged by size:
2057 1925 806 730 611 357 303 190
93 74 62 58 20
BspEI T'CCGG_A
Cuts at: 6506 6506
Size: 7286
BspHI T'CATG_A
Cuts at: 2831 6462 2831
Size: 3631 3655
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3711 4336 4550 4852 5233 3711
Size: 625 214 302 381 5764
Fragments arranged by size:
5764 625 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5578 5632 7116 7281 50
Size: 5528 54 1484 165 55
Fragments arranged by size:
5528 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 226 1005 1215 2456 5198 226
Size: 779 210 1241 2742 2314
Fragments arranged by size:
2742 2314 1241 779 210
BsrFI r'CCGG_y
Cuts at: 3276 3678 5284 5465 5674 3276
Size: 402 1606 181 209 4888
Fragments arranged by size:
4888 1606 402 209 181
BsrGI T'GTAC_A
Cuts at: 3117 3117
Size: 7286
BssSI C'ACGA_G
Cuts at: 2875 3158 5557 5915 2875
Size: 283 2399 358 4246
Fragments arranged by size:
4246 2399 358 283
BstAPI GCAn_nnn'nTGC
Cuts at: 1205 4663 1205
Size: 3458 3828
BstBI TT'CG_AA
Cuts at: 110 5647 110
Size: 5537 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 676 963 3183 3271 3541 4095 676
Size: 287 2220 88 270 554 3867
Fragments arranged by size:
3867 2220 554 287 270 88
BstYI r'GATC_y
Cuts at: 1 495 628 1038 3584 3876 4311 4778 4801
Size: 494 133 410 2546 292 435 467 23
Cuts at: 4801 5136 5382 6394 6480 6492 6838 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
2546 1012 494 467 449 435 410 346
335 292 246 133 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 873 873
Size: 7286
BtgI C'CryG_G
Cuts at: 3283 3283
Size: 7286
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5236 5417 5580 5236
Size: 181 163 6942
Fragments arranged by size:
6942 181 163
BtsI GCAGTG_nn'
Cuts at: 1248 2820 3089 3099 4529 1248
Size: 1572 269 10 1430 4005
Fragments arranged by size:
4005 1572 1430 269 10
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1811 1846 1811
Size: 35 7251
DraI TTT'AAA
Cuts at: 2466 3956 4116 4285 2466
Size: 1490 160 169 5467
Fragments arranged by size:
5467 1490 169 160
DrdI GACnn_nn'nnGTC
Cuts at: 2326 4992 5850 2326
Size: 2666 858 3762
Fragments arranged by size:
3762 2666 858
EaeI y'GGCC_r
Cuts at: 953 1287 3543 3626 3680 4871 5045 5436 5463
Size: 334 2256 83 54 1191 174 391 27
Cuts at: 5463 6808 953
Size: 1345 1431
Fragments arranged by size:
2256 1431 1345 1191 391 334 174 83
54 27
EagI C'GGCC_G
Cuts at: 4871 4871
Size: 7286
EarI CTCTTCn'nnn_
Cuts at: 1478 3941 4230 5309 5519 1478
Size: 2463 289 1079 210 3245
Fragments arranged by size:
3245 2463 1079 289 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3034 5494 5804 5950 7096 3034
Size: 2460 310 146 1146 3224
Fragments arranged by size:
3224 2460 1146 310 146
EcoICRI GAG'CTC
Cuts at: 4298 4298
Size: 7286
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 338 434 835 1000 1433 1691 2696 2783
Size: 93 96 401 165 433 258 1005 87
Cuts at: 2783 3265 3477 5111 5543 6290 6558 245
Size: 482 212 1634 432 747 268 973
Fragments arranged by size:
1634 1005 973 747 482 433 432 401
268 258 212 165 96 93 87
EcoNI CCTnn'n_nnAGG
Cuts at: 667 667
Size: 7286
EcoO109I rG'GnC_Cy
Cuts at: 860 890 921 3098 4158 4425 860
Size: 30 31 2177 1060 267 3721
Fragments arranged by size:
3721 2177 1060 267 31 30
EcoRI G'AATT_C
Cuts at: 867 1583 2838 4290 867
Size: 716 1255 1452 3863
Fragments arranged by size:
3863 1452 1255 716
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1220 2325 2792 3231 5305 1220
Size: 1105 467 439 2074 3201
Fragments arranged by size:
3201 2074 1105 467 439
FspI TGC'GCA
Cuts at: 5067 5067
Size: 7286
HaeII r_GCGC'y
Cuts at: 685 4720 4968 5990 6969 685
Size: 4035 248 1022 979 1002
Fragments arranged by size:
4035 1022 1002 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 327 359 1825 1857 2115 2147 2219 2251 2474
Size: 32 1466 32 258 32 72 32 223
Cuts at: 2474 2492 2506 2524 2950 2982 3033 3065 3157
Size: 18 14 18 426 32 51 32 92
Cuts at: 3157 3189 3569 3601 5128 5158 5160 5190 327
Size: 32 380 32 1527 30 2 30 2423
Fragments arranged by size:
2423 1527 1466 426 380 258 223 92
72 51 32 32 32 32 32 32
32 32 30 30 18 18 14 2
HincII GTy'rAC
Cuts at: 3309 4325 3309
Size: 1016 6270
HindIII A'AGCT_T
Cuts at: 257 1163 2568 4259 257
Size: 906 1405 1691 3284
Fragments arranged by size:
3284 1691 1405 906
KasI G'GCGC_C
Cuts at: 4716 4964 4716
Size: 248 7038
KpnI G_GTAC'C
Cuts at: 4306 4306
Size: 7286
MfeI C'AATT_G
Cuts at: 2819 2819
Size: 7286
MluI A'CGCG_T
Cuts at: 6719 6719
Size: 7286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 192 1027 1384 1560 3200 5957 6141 192
Size: 835 357 176 1640 2757 184 1337
Fragments arranged by size:
2757 1640 1337 835 357 184 176
MscI TGG'CCA
Cuts at: 1289 3545 3628 5047 1289
Size: 2256 83 1419 3528
Fragments arranged by size:
3528 2256 1419 83
MslI CAynn'nnrTG
Cuts at: 1108 1241 1541 1784 1873 2083 2408 3269 3539
Size: 133 300 243 89 210 325 861 270
Cuts at: 3539 4070 5275 5402 1108
Size: 531 1205 127 2992
Fragments arranged by size:
2992 1205 861 531 325 300 270 243
210 133 127 89
MspA1I CmG'CkG
Cuts at: 2161 3393 4194 4397 4711 5071 6084 6329 2161
Size: 1232 801 203 314 360 1013 245 3118
Fragments arranged by size:
3118 1232 1013 801 360 314 245 203
NaeI GCC'GGC
Cuts at: 3278 3680 5467 3278
Size: 402 1787 5097
Fragments arranged by size:
5097 1787 402
NarI GG'CG_CC
Cuts at: 4717 4965 4717
Size: 248 7038
NgoMIV G'CCGG_C
Cuts at: 3276 3678 5465 3276
Size: 402 1787 5097
Fragments arranged by size:
5097 1787 402
NspI r_CATG'y
Cuts at: 1822 2896 4339 4839 5746 1822
Size: 1074 1443 500 907 3362
Fragments arranged by size:
3362 1443 1074 907 500
PciI A'CATG_T
Cuts at: 2892 5742 2892
Size: 2850 4436
PflMI CCAn_nnn'nTGG
Cuts at: 775 1149 1425 1986 3266 775
Size: 374 276 561 1280 4795
Fragments arranged by size:
4795 1280 561 374 276
PfoI T'CCnGG_A
Cuts at: 498 668 1034 1115 498
Size: 170 366 81 6669
Fragments arranged by size:
6669 366 170 81
PmeI GTTT'AAAC
Cuts at: 4285 4285
Size: 7286
PmlI CAC'GTG
Cuts at: 2431 2431
Size: 7286
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6425 6457 6425
Size: 32 7254
PpuMI rG'GwC_Cy
Cuts at: 860 890 3098 860
Size: 30 2208 5048
Fragments arranged by size:
5048 2208 30
PsiI TTA'TAA
Cuts at: 1802 2276 1802
Size: 474 6812
PspOMI G'GGCC_C
Cuts at: 2752 2752
Size: 7286
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4166 4198 4166
Size: 32 7254
PstI C_TGCA'G
Cuts at: 2166 3094 4333 5018 2166
Size: 928 1239 685 4434
Fragments arranged by size:
4434 1239 928 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7286
PvuII CAG'CTG
Cuts at: 2161 4194 4711 5071 2161
Size: 2033 517 360 4376
Fragments arranged by size:
4376 2033 517 360
RsrII CG'GwC_CG
Cuts at: 5481 5481
Size: 7286
SacI G_AGCT'C
Cuts at: 4300 4300
Size: 7286
SalI G'TCGA_C
Cuts at: 4323 4323
Size: 7286
SapI GCTCTTCn'nnn_
Cuts at: 5309 5519 5309
Size: 210 7076
SbfI CC_TGCA'GG
Cuts at: 4333 4333
Size: 7286
ScaI AGT'ACT
Cuts at: 3646 6803 3646
Size: 3157 4129
SfcI C'TryA_G
Cuts at: 33 578 1155 2162 3090 3648 4329 5014 6007
Size: 545 577 1007 928 558 681 685 993
Cuts at: 6007 6198 33
Size: 191 1121
Fragments arranged by size:
1121 1007 993 928 685 681 577 558
545 191
SfoI GGC'GCC
Cuts at: 4718 4966 4718
Size: 248 7038
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7286
SmaI CCC'GGG
Cuts at: 919 4308 919
Size: 3389 3897
SmlI C'TyrA_G
Cuts at: 1860 4441 5356 5848 6110 1860
Size: 2581 915 492 262 3036
Fragments arranged by size:
3036 2581 915 492 262
SnaBI TAC'GTA
Cuts at: 4278 4278
Size: 7286
SpeI A'CTAG_T
Cuts at: 67 391 3733 4035 67
Size: 324 3342 302 3318
Fragments arranged by size:
3342 3318 324 302
SphI G_CATG'C
Cuts at: 4339 4839 4339
Size: 500 6786
SspI AAT'ATT
Cuts at: 639 1599 639
Size: 960 6326
StyI C'CwwG_G
Cuts at: 1143 1608 2359 3973 4161 4420 4774 1143
Size: 465 751 1614 188 259 354 3655
Fragments arranged by size:
3655 1614 751 465 354 259 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 209 1531 4869 5002 5461 5670 209
Size: 1322 3338 133 459 209 1825
Fragments arranged by size:
3338 1825 1322 459 209 133
TatI w'GTAC_w
Cuts at: 3117 3644 6801 3117
Size: 527 3157 3602
Fragments arranged by size:
3602 3157 527
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1461 1493 6803 6835 1461
Size: 32 5310 32 1912
Fragments arranged by size:
5310 1912 32 32
Tth111I GACn'n_nGTC
Cuts at: 3698 5083 3698
Size: 1385 5901
XbaI T'CTAG_A
Cuts at: 4317 4317
Size: 7286
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 175 2983 4781 175
Size: 2808 1798 2680
Fragments arranged by size:
2808 2680 1798
XmaI C'CCGG_G
Cuts at: 917 4306 917
Size: 3389 3897
XmnI GAAnn'nnTTC
Cuts at: 1200 1200
Size: 7286
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AloI AscI AvrII BcgI BfrBI
BglI BmtI BsiWI BssHII BstEII BstZ17I ClaI DraIII
EcoRV FseI FspAI HpaI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PshAI PspXI SacII SanDI
SexAI SfiI SgrAI SrfI StuI SwaI XhoI ZraI