Restriction map for
pF1KSDA1427
(Circular) (Six-base) MAPSORT of: pF1KSDA1427.seq Check: 2133 from: 1 to: 4415
pF1KSDA1427 4415 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 214 465 214
Size: 251 4164
AccI GT'mk_AC
Cuts at: 436 1453 436
Size: 1017 3398
Acc65I G'GTAC_C
Cuts at: 1431 1431
Size: 4415
AclI AA'CG_TT
Cuts at: 4255 4255
Size: 4415
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 947 976 1165 2240 2672 3419 3687 947
Size: 29 189 1075 432 747 268 1675
Fragments arranged by size:
1675 1075 747 432 268 189 29
AflIII A'CryG_T
Cuts at: 1767 2871 3848 1767
Size: 1104 977 2334
Fragments arranged by size:
2334 1104 977
AgeI A'CCGG_T
Cuts at: 2803 2803
Size: 4415
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1304 1304
Size: 4415
AlwNI CAG_nnn'CTG
Cuts at: 988 1343 1720 3287 988
Size: 355 377 1567 2116
Fragments arranged by size:
2116 1567 377 355
ApaLI G'TGCA_C
Cuts at: 3185 3185
Size: 4415
ApoI r'AATT_y
Cuts at: 1419 3768 4024 1419
Size: 2349 256 1810
Fragments arranged by size:
2349 1810 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4415
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4415
AvaI C'yCGr_G
Cuts at: 292 1323 1435 292
Size: 1031 112 3272
Fragments arranged by size:
3272 1031 112
BamHI G'GATC_C
Cuts at: 1440 1440
Size: 4415
BanI G'GyrC_C
Cuts at: 138 157 1388 1431 1845 2093 2128 138
Size: 19 1231 43 414 248 35 2425
Fragments arranged by size:
2425 1231 414 248 43 35 19
BanII G_rGCy'C
Cuts at: 449 827 899 1091 1330 1429 2459 449
Size: 378 72 192 239 99 1030 2405
Fragments arranged by size:
2405 1030 378 239 192 99 72
BbeI G_GCGC'C
Cuts at: 161 1849 2097 161
Size: 1688 248 2479
Fragments arranged by size:
2479 1688 248
BbsI GAAGACnn'nnnn_
Cuts at: 964 964
Size: 4415
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1117 1151 1117
Size: 34 4381
BciVI GTATCCnnnnn_n'
Cuts at: 2313 3074 4415 2313
Size: 761 1341 2313
Fragments arranged by size:
2313 1341 761
BclI T'GATC_A
Cuts at: 1202 1935 1202
Size: 733 3682
BglII A'GATC_T
Cuts at: 1930 3967 1930
Size: 2037 2378
BlpI GC'TnA_GC
Cuts at: 1169 1527 1169
Size: 358 4057
Bme1580I G_kGCm'C
Cuts at: 172 351 2040 2133 3189 172
Size: 179 1689 93 1056 1398
Fragments arranged by size:
1689 1398 1056 179 93
BmrI ACTGGGnnnn_n'
Cuts at: 936 1353 1627 1708 2043 4187 936
Size: 417 274 81 335 2144 1164
Fragments arranged by size:
2144 1164 417 335 274 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2782 2782
Size: 4415
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 383 1010 1224 289
Size: 94 627 214 3480
Fragments arranged by size:
3480 627 214 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 475 873 1591 2470 2962 3260 340
Size: 135 398 718 879 492 298 1495
Fragments arranged by size:
1495 879 718 492 398 298 135
BsaI GGTCTCn'nnnn_
Cuts at: 556 4119 556
Size: 3563 852
BsaAI yAC'GTr
Cuts at: 1407 1768 1407
Size: 361 4054
BsaBI GATnn'nnATC
Cuts at: 6 1929 3966 6
Size: 1923 2037 455
Fragments arranged by size:
2037 1923 455
BsaHI Gr'CG_yC
Cuts at: 158 1846 2094 2796 4292 158
Size: 1688 248 702 1496 281
Fragments arranged by size:
1688 1496 702 281 248
BsaWI w'CCGG_w
Cuts at: 1604 2125 2803 3077 3224 3635 1604
Size: 521 678 274 147 411 2384
Fragments arranged by size:
2384 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 856 886 856
Size: 30 4385
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 343 457 523 805 832 835 1363 343
Size: 114 66 282 27 3 528 3395
Fragments arranged by size:
3395 528 282 114 66 27 3
BseYI C'CCAG_C
Cuts at: 153 165 283 1260 1840 2598 3175 3671 153
Size: 12 118 977 580 758 577 496 897
Fragments arranged by size:
977 897 758 580 577 496 118 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 437 760 1300 1381 284
Size: 153 323 540 81 3318
Fragments arranged by size:
3318 540 323 153 81
BsiEI CG_ry'CG
Cuts at: 104 867 1957 2003 3211 104
Size: 763 1090 46 1208 1308
Fragments arranged by size:
1308 1208 1090 763 46
BsiHKAI G_wGCw'C
Cuts at: 806 827 899 1091 1429 2207 2397 3189 806
Size: 21 72 192 338 778 190 792 2032
Fragments arranged by size:
2032 792 778 338 192 190 72 21
Bsp1286I G_dGCh'C
Cuts at: 172 351 449 806 827 899 1091 1330 1429
Size: 179 98 357 21 72 192 239 99
Cuts at: 1429 2040 2133 2207 2397 2459 3189 172
Size: 611 93 74 190 62 730 1398
Fragments arranged by size:
1398 730 611 357 239 192 190 179
99 98 93 74 72 62 21
BspEI T'CCGG_A
Cuts at: 3635 3635
Size: 4415
BspHI T'CATG_A
Cuts at: 3591 3591
Size: 4415
BspMI ACCTGCnnnn'nnnn_
Cuts at: 214 465 489 868 1235 1465 1679 1981 2362
Size: 251 24 379 367 230 214 302 381
Cuts at: 2362 214
Size: 2267
Fragments arranged by size:
2267 381 379 367 302 251 230 214
24
BsrBI CCG'CTC
Cuts at: 50 2707 2761 4245 4410 50
Size: 2657 54 1484 165 55
Fragments arranged by size:
2657 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2327 2327
Size: 4415
BsrFI r'CCGG_y
Cuts at: 733 1370 2413 2594 2803 733
Size: 637 1043 181 209 2345
Fragments arranged by size:
2345 1043 637 209 181
BssSI C'ACGA_G
Cuts at: 2686 3044 2686
Size: 358 4057
BstAPI GCAn_nnn'nTGC
Cuts at: 1395 1792 1395
Size: 397 4018
BstBI TT'CG_AA
Cuts at: 110 2776 110
Size: 2666 1749
BstEII G'GTnAC_C
Cuts at: 201 1116 201
Size: 915 3500
BstYI r'GATC_y
Cuts at: 1 1440 1907 1930 2265 2511 3523 3609 3621
Size: 1439 467 23 335 246 1012 86 12
Cuts at: 3621 3967 1
Size: 346 449
Fragments arranged by size:
1439 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 125 247 326 125
Size: 122 79 4214
Fragments arranged by size:
4214 122 79
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2365 2546 2709 2365
Size: 181 163 4071
Fragments arranged by size:
4071 181 163
BtsI GCAGTG_nn'
Cuts at: 449 1299 1658 449
Size: 850 359 3206
Fragments arranged by size:
3206 850 359
DraI TTT'AAA
Cuts at: 1414 1414
Size: 4415
DraIII CAC_nnn'GTG
Cuts at: 885 885
Size: 4415
DrdI GACnn_nn'nnGTC
Cuts at: 706 2121 2979 706
Size: 1415 858 2142
Fragments arranged by size:
2142 1415 858
EaeI y'GGCC_r
Cuts at: 722 887 1296 2000 2174 2565 2592 3937 722
Size: 165 409 704 174 391 27 1345 1200
Fragments arranged by size:
1345 1200 704 409 391 174 165 27
EagI C'GGCC_G
Cuts at: 2000 2000
Size: 4415
EarI CTCTTCn'nnn_
Cuts at: 2438 2648 2438
Size: 210 4205
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 829 889 2623 2933 3079 4225 829
Size: 60 1734 310 146 1146 1019
Fragments arranged by size:
1734 1146 1019 310 146 60
EcoICRI GAG'CTC
Cuts at: 825 897 1089 1427 825
Size: 72 192 338 3813
Fragments arranged by size:
3813 338 192 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 383 947 976 1010 1165 1224 2240 2672
Size: 94 564 29 34 155 59 1016 432
Cuts at: 2672 3419 3687 289
Size: 747 268 1017
Fragments arranged by size:
1017 1016 747 564 432 268 155 94
59 34 29
EcoNI CCTnn'n_nnAGG
Cuts at: 271 814 1096 1231 271
Size: 543 282 135 3455
Fragments arranged by size:
3455 543 282 135
EcoO109I rG'GnC_Cy
Cuts at: 258 600 795 857 996 1554 258
Size: 342 195 62 139 558 3119
Fragments arranged by size:
3119 558 342 195 139 62
EcoRI G'AATT_C
Cuts at: 1419 1419
Size: 4415
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 303 1137 2434 303
Size: 834 1297 2284
Fragments arranged by size:
2284 1297 834
FspI TGC'GCA
Cuts at: 2196 2196
Size: 4415
HaeII r_GCGC'y
Cuts at: 161 1849 2097 3119 4098 161
Size: 1688 248 1022 979 478
Fragments arranged by size:
1688 1022 979 478 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 856 888 904 936 2257 2287 2289 2319 856
Size: 32 16 32 1321 30 2 30 2952
Fragments arranged by size:
2952 1321 32 32 30 30 16 2
HincII GTy'rAC
Cuts at: 1454 1454
Size: 4415
KasI G'GCGC_C
Cuts at: 157 1845 2093 157
Size: 1688 248 2479
Fragments arranged by size:
2479 1688 248
KpnI G_GTAC'C
Cuts at: 1435 1435
Size: 4415
MluI A'CGCG_T
Cuts at: 3848 3848
Size: 4415
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 571 3086 3270 571
Size: 2515 184 1716
Fragments arranged by size:
2515 1716 184
MscI TGG'CCA
Cuts at: 724 1298 2176 724
Size: 574 878 2963
Fragments arranged by size:
2963 878 574
MslI CAynn'nnrTG
Cuts at: 2404 2531 2404
Size: 127 4288
MspA1I CmG'CkG
Cuts at: 249 388 774 1395 1526 1840 2200 3213 3458
Size: 139 386 621 131 314 360 1013 245
Cuts at: 3458 249
Size: 1206
Fragments arranged by size:
1206 1013 621 386 360 314 245 139
131
NaeI GCC'GGC
Cuts at: 1372 2596 1372
Size: 1224 3191
NarI GG'CG_CC
Cuts at: 158 1846 2094 158
Size: 1688 248 2479
Fragments arranged by size:
2479 1688 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 4415
NgoMIV G'CCGG_C
Cuts at: 1370 2594 1370
Size: 1224 3191
NspI r_CATG'y
Cuts at: 225 1468 1968 2875 225
Size: 1243 500 907 1765
Fragments arranged by size:
1765 1243 907 500
PciI A'CATG_T
Cuts at: 2871 2871
Size: 4415
PmeI GTTT'AAAC
Cuts at: 1414 1414
Size: 4415
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 499 531 1054 1086 3554 3586 499
Size: 32 523 32 2468 32 1328
Fragments arranged by size:
2468 1328 523 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 258 857 996 258
Size: 599 139 3677
Fragments arranged by size:
3677 599 139
PstI C_TGCA'G
Cuts at: 984 1235 1306 1462 2147 984
Size: 251 71 156 685 3252
Fragments arranged by size:
3252 685 251 156 71
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4415
PvuII CAG'CTG
Cuts at: 388 774 1395 1840 2200 388
Size: 386 621 445 360 2603
Fragments arranged by size:
2603 621 445 386 360
RsrII CG'GwC_CG
Cuts at: 2610 2610
Size: 4415
SacI G_AGCT'C
Cuts at: 827 899 1091 1429 827
Size: 72 192 338 3813
Fragments arranged by size:
3813 338 192 72
SacII CC_GC'GG
Cuts at: 250 250
Size: 4415
SalI G'TCGA_C
Cuts at: 1452 1452
Size: 4415
SapI GCTCTTCn'nnn_
Cuts at: 2438 2648 2438
Size: 210 4205
SbfI CC_TGCA'GG
Cuts at: 1462 1462
Size: 4415
ScaI AGT'ACT
Cuts at: 3932 3932
Size: 4415
SfcI C'TryA_G
Cuts at: 33 980 1231 1302 1458 2143 3136 3327 33
Size: 947 251 71 156 685 993 191 1121
Fragments arranged by size:
1121 993 947 685 251 191 156 71
SfoI GGC'GCC
Cuts at: 159 1847 2095 159
Size: 1688 248 2479
Fragments arranged by size:
2479 1688 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4415
SmaI CCC'GGG
Cuts at: 1437 1437
Size: 4415
SmlI C'TyrA_G
Cuts at: 355 490 852 1570 2485 2977 3239 355
Size: 135 362 718 915 492 262 1531
Fragments arranged by size:
1531 915 718 492 362 262 135
SnaBI TAC'GTA
Cuts at: 1407 1407
Size: 4415
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4415
SphI G_CATG'C
Cuts at: 1468 1968 1468
Size: 500 3915
StuI AGG'CCT
Cuts at: 1237 1237
Size: 4415
StyI C'CwwG_G
Cuts at: 125 711 1082 1549 1903 125
Size: 586 371 467 354 2637
Fragments arranged by size:
2637 586 467 371 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1998 2131 2590 2799 1998
Size: 133 459 209 3614
Fragments arranged by size:
3614 459 209 133
TatI w'GTAC_w
Cuts at: 3930 3930
Size: 4415
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3932 3964 3932
Size: 32 4383
Tth111I GACn'n_nGTC
Cuts at: 2212 2212
Size: 4415
XbaI T'CTAG_A
Cuts at: 1446 1446
Size: 4415
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1035 1089 1248 1910 1035
Size: 54 159 662 3540
Fragments arranged by size:
3540 662 159 54
XmaI C'CCGG_G
Cuts at: 1435 1435
Size: 4415
XmnI GAAnn'nnTTC
Cuts at: 335 335
Size: 4415
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BsiWI BsmI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SanDI
SexAI SfiI SgrAI SrfI SspI SwaI XhoI ZraI