Restriction map for
pF1KSDA1433
(Circular) (Six-base) MAPSORT of: pF1KSDA1433.seq Check: 2959 from: 1 to: 5054
pF1KSDA1433 5054 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 2092 2092
Size: 5054
Acc65I G'GTAC_C
Cuts at: 2070 2070
Size: 5054
AclI AA'CG_TT
Cuts at: 4894 4894
Size: 5054
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 527 2879 3311 4058 4326 337
Size: 190 2352 432 747 268 1065
Fragments arranged by size:
2352 1065 747 432 268 190
AfeI AGC'GCT
Cuts at: 640 640
Size: 5054
AflII C'TTAA_G
Cuts at: 373 373
Size: 5054
AflIII A'CryG_T
Cuts at: 2406 3510 4487 2406
Size: 1104 977 2973
Fragments arranged by size:
2973 1104 977
AgeI A'CCGG_T
Cuts at: 3442 3442
Size: 5054
AleI CACnn'nnGTG
Cuts at: 1645 1645
Size: 5054
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 418 506 418
Size: 88 4966
AlwNI CAG_nnn'CTG
Cuts at: 1030 1979 2359 3926 1030
Size: 949 380 1567 2158
Fragments arranged by size:
2158 1567 949 380
ApaLI G'TGCA_C
Cuts at: 3824 3824
Size: 5054
ApoI r'AATT_y
Cuts at: 568 1519 2058 4407 4663 568
Size: 951 539 2349 256 959
Fragments arranged by size:
2349 959 951 539 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5054
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5054
AvaI C'yCGr_G
Cuts at: 2074 2074
Size: 5054
BamHI G'GATC_C
Cuts at: 2079 2079
Size: 5054
BanI G'GyrC_C
Cuts at: 446 2070 2484 2732 2767 446
Size: 1624 414 248 35 2733
Fragments arranged by size:
2733 1624 414 248 35
BanII G_rGCy'C
Cuts at: 1360 1570 1807 2068 3098 1360
Size: 210 237 261 1030 3316
Fragments arranged by size:
3316 1030 261 237 210
BbeI G_GCGC'C
Cuts at: 2488 2736 2488
Size: 248 4806
BbsI GAAGACnn'nnnn_
Cuts at: 1970 1970
Size: 5054
BciVI GTATCCnnnnn_n'
Cuts at: 492 2952 3713 5054 492
Size: 2460 761 1341 492
Fragments arranged by size:
2460 1341 761 492
BclI T'GATC_A
Cuts at: 2574 2574
Size: 5054
BglI GCCn_nnn'nGGC
Cuts at: 223 223
Size: 5054
BglII A'GATC_T
Cuts at: 2569 4606 2569
Size: 2037 3017
BlpI GC'TnA_GC
Cuts at: 733 2166 733
Size: 1433 3621
Bme1580I G_kGCm'C
Cuts at: 2679 2772 3828 2679
Size: 93 1056 3905
Fragments arranged by size:
3905 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1133 1328 2266 2347 2682 4826 1133
Size: 195 938 81 335 2144 1361
Fragments arranged by size:
2144 1361 938 335 195 81
BmtI G_CTAG'C
Cuts at: 1454 1454
Size: 5054
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 572 657 3421 572
Size: 85 2764 2205
Fragments arranged by size:
2764 2205 85
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1304 1638 1763 1799 1304
Size: 334 125 36 4559
Fragments arranged by size:
4559 334 125 36
Bpu10I CC'TnA_GC
Cuts at: 1801 1801
Size: 5054
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 697 2230 3109 3601 3899 490
Size: 207 1533 879 492 298 1645
Fragments arranged by size:
1645 1533 879 492 298 207
BsaI GGTCTCn'nnnn_
Cuts at: 4758 4758
Size: 5054
BsaAI yAC'GTr
Cuts at: 2046 2407 2046
Size: 361 4693
BsaBI GATnn'nnATC
Cuts at: 6 2568 4605 6
Size: 2562 2037 455
Fragments arranged by size:
2562 2037 455
BsaHI Gr'CG_yC
Cuts at: 2485 2733 3435 4931 2485
Size: 248 702 1496 2608
Fragments arranged by size:
2608 1496 702 248
BsaWI w'CCGG_w
Cuts at: 899 2243 2764 3442 3716 3863 4274 899
Size: 1344 521 678 274 147 411 1679
Fragments arranged by size:
1679 1344 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 503 533 1386 1416 1884 1914 503
Size: 30 853 30 468 30 3643
Fragments arranged by size:
3643 853 468 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 649 1374 1377 649
Size: 725 3 4326
Fragments arranged by size:
4326 725 3
BseYI C'CCAG_C
Cuts at: 422 1369 2479 3237 3814 4310 422
Size: 947 1110 758 577 496 1166
Fragments arranged by size:
1166 1110 947 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 336 336
Size: 5054
BsiEI CG_ry'CG
Cuts at: 104 2596 2642 3850 104
Size: 2492 46 1208 1308
Fragments arranged by size:
2492 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1360 1652 2068 2846 3036 3828 1360
Size: 292 416 778 190 792 2586
Fragments arranged by size:
2586 792 778 416 292 190
Bsp1286I G_dGCh'C
Cuts at: 1360 1570 1652 1807 2068 2679 2772 2846 3036
Size: 210 82 155 261 611 93 74 190
Cuts at: 3036 3098 3828 1360
Size: 62 730 2586
Fragments arranged by size:
2586 730 611 261 210 190 155 93
82 74 62
BspEI T'CCGG_A
Cuts at: 4274 4274
Size: 5054
BspHI T'CATG_A
Cuts at: 4230 4230
Size: 5054
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2104 2318 2620 3001 2104
Size: 214 302 381 4157
Fragments arranged by size:
4157 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3346 3400 4884 5049 50
Size: 3296 54 1484 165 55
Fragments arranged by size:
3296 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2966 2966
Size: 5054
BsrFI r'CCGG_y
Cuts at: 1416 3052 3233 3442 1416
Size: 1636 181 209 3028
Fragments arranged by size:
3028 1636 209 181
BssSI C'ACGA_G
Cuts at: 3325 3683 3325
Size: 358 4696
BstAPI GCAn_nnn'nTGC
Cuts at: 2431 2431
Size: 5054
BstBI TT'CG_AA
Cuts at: 110 3415 110
Size: 3305 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 132 1623 132
Size: 1491 3563
BstYI r'GATC_y
Cuts at: 1 2079 2546 2569 2904 3150 4162 4248 4260
Size: 2078 467 23 335 246 1012 86 12
Cuts at: 4260 4606 1
Size: 346 449
Fragments arranged by size:
2078 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 1092 1246 1092
Size: 154 4900
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3004 3185 3348 3004
Size: 181 163 4710
Fragments arranged by size:
4710 181 163
BtsI GCAGTG_nn'
Cuts at: 397 1376 1739 1955 2297 397
Size: 979 363 216 342 3154
Fragments arranged by size:
3154 979 363 342 216
DraI TTT'AAA
Cuts at: 935 2053 935
Size: 1118 3936
DrdI GACnn_nn'nnGTC
Cuts at: 2760 3618 2760
Size: 858 4196
EaeI y'GGCC_r
Cuts at: 1173 2639 2813 3204 3231 4576 1173
Size: 1466 174 391 27 1345 1651
Fragments arranged by size:
1651 1466 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2639 2639
Size: 5054
EarI CTCTTCn'nnn_
Cuts at: 626 751 776 887 1350 1416 1989 3077 3287
Size: 125 25 111 463 66 573 1088 210
Cuts at: 3287 626
Size: 2393
Fragments arranged by size:
2393 1088 573 463 210 125 111 66
25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3262 3572 3718 4864 3262
Size: 310 146 1146 3452
Fragments arranged by size:
3452 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1358 2066 1358
Size: 708 4346
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 527 1304 1638 1763 1799 2879 3311 4058
Size: 190 777 334 125 36 1080 432 747
Cuts at: 4058 4326 337
Size: 268 1065
Fragments arranged by size:
1080 1065 777 747 432 334 268 190
125 36
EcoNI CCTnn'n_nnAGG
Cuts at: 1778 1778
Size: 5054
EcoO109I rG'GnC_Cy
Cuts at: 201 951 2193 201
Size: 750 1242 3062
Fragments arranged by size:
3062 1242 750
EcoRI G'AATT_C
Cuts at: 2058 2058
Size: 5054
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 205 825 3073 205
Size: 620 2248 2186
Fragments arranged by size:
2248 2186 620
FspI TGC'GCA
Cuts at: 2835 2835
Size: 5054
HaeII r_GCGC'y
Cuts at: 642 2488 2736 3758 4737 642
Size: 1846 248 1022 979 959
Fragments arranged by size:
1846 1022 979 959 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 503 535 1302 1334 1914 1946 2896 2926 2928
Size: 32 767 32 580 32 950 30 2
Cuts at: 2928 2958 503
Size: 30 2599
Fragments arranged by size:
2599 950 767 580 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2093 2093
Size: 5054
KasI G'GCGC_C
Cuts at: 2484 2732 2484
Size: 248 4806
KpnI G_GTAC'C
Cuts at: 2074 2074
Size: 5054
MfeI C'AATT_G
Cuts at: 991 991
Size: 5054
MluI A'CGCG_T
Cuts at: 4487 4487
Size: 5054
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 545 3725 3909 545
Size: 3180 184 1690
Fragments arranged by size:
3180 1690 184
MscI TGG'CCA
Cuts at: 1175 2815 1175
Size: 1640 3414
MslI CAynn'nnrTG
Cuts at: 526 670 1045 1645 3043 3170 526
Size: 144 375 600 1398 127 2410
Fragments arranged by size:
2410 1398 600 375 144 127
MspA1I CmG'CkG
Cuts at: 426 486 627 793 1623 1979 2165 2479 2839
Size: 60 141 166 830 356 186 314 360
Cuts at: 2839 3852 4097 426
Size: 1013 245 1383
Fragments arranged by size:
1383 1013 830 360 356 314 245 186
166 141 60
NaeI GCC'GGC
Cuts at: 3235 3235
Size: 5054
NarI GG'CG_CC
Cuts at: 2485 2733 2485
Size: 248 4806
NcoI C'CATG_G
Cuts at: 1092 1092
Size: 5054
NdeI CA'TA_TG
Cuts at: 1158 1158
Size: 5054
NgoMIV G'CCGG_C
Cuts at: 3233 3233
Size: 5054
NheI G'CTAG_C
Cuts at: 1450 1450
Size: 5054
NspI r_CATG'y
Cuts at: 405 417 493 531 2107 2607 3514 405
Size: 12 76 38 1576 500 907 1945
Fragments arranged by size:
1945 1576 907 500 76 38 12
PciI A'CATG_T
Cuts at: 3510 3510
Size: 5054
PfoI T'CCnGG_A
Cuts at: 1815 1815
Size: 5054
PmeI GTTT'AAAC
Cuts at: 2053 2053
Size: 5054
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4193 4225 4193
Size: 32 5022
PpuMI rG'GwC_Cy
Cuts at: 201 951 201
Size: 750 4304
PshAI GACnn'nnGTC
Cuts at: 1321 1321
Size: 5054
PstI C_TGCA'G
Cuts at: 1218 1962 2101 2786 1218
Size: 744 139 685 3486
Fragments arranged by size:
3486 744 685 139
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5054
PvuII CAG'CTG
Cuts at: 426 627 793 1623 1979 2479 2839 426
Size: 201 166 830 356 500 360 2641
Fragments arranged by size:
2641 830 500 360 356 201 166
RsrII CG'GwC_CG
Cuts at: 3249 3249
Size: 5054
SacI G_AGCT'C
Cuts at: 1360 2068 1360
Size: 708 4346
SalI G'TCGA_C
Cuts at: 2091 2091
Size: 5054
SapI GCTCTTCn'nnn_
Cuts at: 751 1416 1989 3077 3287 751
Size: 665 573 1088 210 2518
Fragments arranged by size:
2518 1088 665 573 210
SbfI CC_TGCA'GG
Cuts at: 2101 2101
Size: 5054
ScaI AGT'ACT
Cuts at: 4571 4571
Size: 5054
SfcI C'TryA_G
Cuts at: 33 295 1214 1561 1958 2097 2782 3775 3966
Size: 262 919 347 397 139 685 993 191
Cuts at: 3966 33
Size: 1121
Fragments arranged by size:
1121 993 919 685 397 347 262 191
139
SfoI GGC'GCC
Cuts at: 2486 2734 2486
Size: 248 4806
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5054
SmaI CCC'GGG
Cuts at: 2076 2076
Size: 5054
SmlI C'TyrA_G
Cuts at: 373 469 676 2209 3124 3616 3878 373
Size: 96 207 1533 915 492 262 1549
Fragments arranged by size:
1549 1533 915 492 262 207 96
SnaBI TAC'GTA
Cuts at: 2046 2046
Size: 5054
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5054
SphI G_CATG'C
Cuts at: 417 493 2107 2607 417
Size: 76 1614 500 2864
Fragments arranged by size:
2864 1614 500 76
StyI C'CwwG_G
Cuts at: 1092 2188 2542 1092
Size: 1096 354 3604
Fragments arranged by size:
3604 1096 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1286 1881 1890 2637 2770 3229 3438 1286
Size: 595 9 747 133 459 209 2902
Fragments arranged by size:
2902 747 595 459 209 133 9
TatI w'GTAC_w
Cuts at: 4569 4569
Size: 5054
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1731 1763 1884 1916 4571 4603 1731
Size: 32 121 32 2655 32 2182
Fragments arranged by size:
2655 2182 121 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2851 2851
Size: 5054
XbaI T'CTAG_A
Cuts at: 2085 2085
Size: 5054
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1552 2549 1552
Size: 997 4057
XmaI C'CCGG_G
Cuts at: 2074 2074
Size: 5054
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NspI PciI PfoI PmeI PpiI PpuMI PshAI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AhdI AloI ApaI AscI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI NotI NruI NsiI PacI
PasI PflMI PmlI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SrfI SspI StuI SwaI
XhoI XmnI ZraI