Restriction map for
pF1KSDA1436
(Circular) (Six-base) MAPSORT of: pF1KSDA1436.seq Check: 4139 from: 1 to: 5774
pF1KSDA1436 5774 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AatII G_ACGT'C
Cuts at: 1052 1052
Size: 5774
AccI GT'mk_AC
Cuts at: 2812 2812
Size: 5774
Acc65I G'GTAC_C
Cuts at: 2790 2790
Size: 5774
AclI AA'CG_TT
Cuts at: 5614 5614
Size: 5774
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 443 1016 1059 3599 4031 4778 5046 443
Size: 573 43 2540 432 747 268 1171
Fragments arranged by size:
2540 1171 747 573 432 268 43
AfeI AGC'GCT
Cuts at: 766 766
Size: 5774
AflIII A'CryG_T
Cuts at: 1247 1613 3126 4230 5207 1247
Size: 366 1513 1104 977 1814
Fragments arranged by size:
1814 1513 1104 977 366
AgeI A'CCGG_T
Cuts at: 4162 4162
Size: 5774
AleI CACnn'nnGTG
Cuts at: 940 940
Size: 5774
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 340 372 2020 2052 340
Size: 32 1648 32 4062
Fragments arranged by size:
4062 1648 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1280 3079 4646 1280
Size: 1799 1567 2408
Fragments arranged by size:
2408 1799 1567
ApaI G_GGCC'C
Cuts at: 571 1575 571
Size: 1004 4770
ApaLI G'TGCA_C
Cuts at: 685 1144 2326 4544 685
Size: 459 1182 2218 1915
Fragments arranged by size:
2218 1915 1182 459
ApoI r'AATT_y
Cuts at: 2449 2778 5127 5383 2449
Size: 329 2349 256 2840
Fragments arranged by size:
2840 2349 329 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5774
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5774
AvaI C'yCGr_G
Cuts at: 141 744 830 1057 1378 2794 141
Size: 603 86 227 321 1416 3121
Fragments arranged by size:
3121 1416 603 321 227 86
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 849 882 849
Size: 33 5741
BamHI G'GATC_C
Cuts at: 1628 2286 2799 1628
Size: 658 513 4603
Fragments arranged by size:
4603 658 513
BanI G'GyrC_C
Cuts at: 131 1260 2790 3204 3452 3487 131
Size: 1129 1530 414 248 35 2418
Fragments arranged by size:
2418 1530 1129 414 248 35
BanII G_rGCy'C
Cuts at: 437 571 617 837 856 1575 2660 2788 3818
Size: 134 46 220 19 719 1085 128 1030
Cuts at: 3818 437
Size: 2393
Fragments arranged by size:
2393 1085 1030 719 220 134 128 46
19
BbeI G_GCGC'C
Cuts at: 135 3208 3456 135
Size: 3073 248 2453
Fragments arranged by size:
3073 2453 248
BbsI GAAGACnn'nnnn_
Cuts at: 1298 1655 1719 2357 1298
Size: 357 64 638 4715
Fragments arranged by size:
4715 638 357 64
BbvCI CC'TCA_GC
Cuts at: 2422 2422
Size: 5774
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 132 166 1498 1532 132
Size: 34 1332 34 4374
Fragments arranged by size:
4374 1332 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2627 3672 4433 5774 2627
Size: 1045 761 1341 2627
Fragments arranged by size:
2627 1341 1045 761
BclI T'GATC_A
Cuts at: 2243 3294 2243
Size: 1051 4723
BfrBI ATG'CAT
Cuts at: 1990 1990
Size: 5774
BglII A'GATC_T
Cuts at: 2238 3289 5326 2238
Size: 1051 2037 2686
Fragments arranged by size:
2686 2037 1051
BlpI GC'TnA_GC
Cuts at: 2886 2886
Size: 5774
Bme1580I G_kGCm'C
Cuts at: 235 571 689 977 1148 1263 1575 2330 3399
Size: 336 118 288 171 115 312 755 1069
Cuts at: 3399 3492 4548 235
Size: 93 1056 1461
Fragments arranged by size:
1461 1069 1056 755 336 312 288 171
118 115 93
BmgBI CAC'GTC
Cuts at: 783 1067 783
Size: 284 5490
BmrI ACTGGGnnnn_n'
Cuts at: 2986 3067 3402 5546 2986
Size: 81 335 2144 3214
Fragments arranged by size:
3214 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2580 4141 2580
Size: 1561 4213
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 318 1899 1993 2449 318
Size: 1581 94 456 3643
Fragments arranged by size:
3643 1581 456 94
Bpu10I CC'TnA_GC
Cuts at: 595 2422 595
Size: 1827 3947
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2950 3829 4321 4619 2950
Size: 879 492 298 4105
Fragments arranged by size:
4105 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2070 2111 5478 2070
Size: 41 3367 2366
Fragments arranged by size:
3367 2366 41
BsaAI yAC'GTr
Cuts at: 564 1248 2766 3127 564
Size: 684 1518 361 3211
Fragments arranged by size:
3211 1518 684 361
BsaBI GATnn'nnATC
Cuts at: 6 3288 5325 6
Size: 3282 2037 455
Fragments arranged by size:
3282 2037 455
BsaHI Gr'CG_yC
Cuts at: 132 425 809 1049 2633 3205 3453 4155 5651
Size: 293 384 240 1584 572 248 702 1496
Cuts at: 5651 132
Size: 255
Fragments arranged by size:
1584 1496 702 572 384 293 255 248
240
BsaWI w'CCGG_w
Cuts at: 1625 2963 3484 4162 4436 4583 4994 1625
Size: 1338 521 678 274 147 411 2405
Fragments arranged by size:
2405 1338 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 340 370 838 868 2020 2050 2671 2701 340
Size: 30 468 30 1152 30 621 30 3413
Fragments arranged by size:
3413 1152 621 468 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1572 1572
Size: 5774
BseYI C'CCAG_C
Cuts at: 277 360 365 490 571 1311 1706 3199 3957
Size: 83 5 125 81 740 395 1493 758
Cuts at: 3957 4534 5030 277
Size: 577 496 1021
Fragments arranged by size:
1493 1021 758 740 577 496 395 125
83 81 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 553 615 640 2044 544
Size: 9 62 25 1404 4274
Fragments arranged by size:
4274 1404 62 25 9
BsiEI CG_ry'CG
Cuts at: 104 3316 3362 4570 104
Size: 3212 46 1208 1308
Fragments arranged by size:
3212 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 437 689 1148 2330 2360 2788 3566 3756 4548
Size: 252 459 1182 30 428 778 190 792
Cuts at: 4548 437
Size: 1663
Fragments arranged by size:
1663 1182 792 778 459 428 252 190
30
BsmI GAATG_Cn'
Cuts at: 1368 2208 1368
Size: 840 4934
Bsp1286I G_dGCh'C
Cuts at: 235 437 571 617 689 837 856 977 1148
Size: 202 134 46 72 148 19 121 171
Cuts at: 1148 1263 1575 2330 2360 2660 2788 3399 3492
Size: 115 312 755 30 300 128 611 93
Cuts at: 3492 3566 3756 3818 4548 235
Size: 74 190 62 730 1461
Fragments arranged by size:
1461 755 730 611 312 300 202 190
171 148 134 128 121 115 93 74
72 62 46 30 19
BspEI T'CCGG_A
Cuts at: 1625 4994 1625
Size: 3369 2405
BspHI T'CATG_A
Cuts at: 4950 4950
Size: 5774
BspMI ACCTGCnnnn'nnnn_
Cuts at: 525 725 2824 3038 3340 3721 525
Size: 200 2099 214 302 381 2578
Fragments arranged by size:
2578 2099 381 302 214 200
BsrBI CCG'CTC
Cuts at: 50 381 4066 4120 5604 5769 50
Size: 331 3685 54 1484 165 55
Fragments arranged by size:
3685 1484 331 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3686 3686
Size: 5774
BsrFI r'CCGG_y
Cuts at: 1481 3772 3953 4162 1481
Size: 2291 181 209 3093
Fragments arranged by size:
3093 2291 209 181
BssHII G'CGCG_C
Cuts at: 575 575
Size: 5774
BssSI C'ACGA_G
Cuts at: 727 4045 4403 727
Size: 3318 358 2098
Fragments arranged by size:
3318 2098 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3151 3151
Size: 5774
BstBI TT'CG_AA
Cuts at: 110 4135 110
Size: 4025 1749
BstEII G'GTnAC_C
Cuts at: 1500 1500
Size: 5774
BstXI CCAn_nnnn'nTGG
Cuts at: 1404 2488 1404
Size: 1084 4690
BstYI r'GATC_y
Cuts at: 1 398 1628 2238 2286 2558 2799 3266 3289
Size: 397 1230 610 48 272 241 467 23
Cuts at: 3289 3624 3870 4882 4968 4980 5326 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1230 1012 610 467 449 397 346 335
272 246 241 86 48 23 12
Bsu36I CC'TnA_GG
Cuts at: 2181 2181
Size: 5774
BtgI C'CryG_G
Cuts at: 125 428 690 797 923 938 2515 2645 125
Size: 303 262 107 126 15 1577 130 3254
Fragments arranged by size:
3254 1577 303 262 130 126 107 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3724 3905 4068 3724
Size: 181 163 5430
Fragments arranged by size:
5430 181 163
BtsI GCAGTG_nn'
Cuts at: 632 3017 632
Size: 2385 3389
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2680 2715 2680
Size: 35 5739
DraI TTT'AAA
Cuts at: 2773 2773
Size: 5774
DraIII CAC_nnn'GTG
Cuts at: 1290 1290
Size: 5774
DrdI GACnn_nn'nnGTC
Cuts at: 2103 3480 4338 2103
Size: 1377 858 3539
Fragments arranged by size:
3539 1377 858
EaeI y'GGCC_r
Cuts at: 548 698 3359 3533 3924 3951 5296 548
Size: 150 2661 174 391 27 1345 1026
Fragments arranged by size:
2661 1345 1026 391 174 150 27
EagI C'GGCC_G
Cuts at: 3359 3359
Size: 5774
EarI CTCTTCn'nnn_
Cuts at: 1423 2407 3797 4007 1423
Size: 984 1390 210 3190
Fragments arranged by size:
3190 1390 984 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 426 633 720 3982 4292 4438 5584 426
Size: 207 87 3262 310 146 1146 616
Fragments arranged by size:
3262 1146 616 310 207 146 87
EcoICRI GAG'CTC
Cuts at: 435 2786 435
Size: 2351 3423
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 318 443 1016 1059 1899 1993 2449 3599 4031
Size: 125 573 43 840 94 456 1150 432
Cuts at: 4031 4778 5046 318
Size: 747 268 1046
Fragments arranged by size:
1150 1046 840 747 573 456 432 268
125 94 43
EcoNI CCTnn'n_nnAGG
Cuts at: 604 1077 2344 2400 604
Size: 473 1267 56 3978
Fragments arranged by size:
3978 1267 473 56
EcoO109I rG'GnC_Cy
Cuts at: 568 1374 2185 2913 568
Size: 806 811 728 3429
Fragments arranged by size:
3429 811 806 728
EcoRI G'AATT_C
Cuts at: 2449 2778 2449
Size: 329 5445
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1743 1821 2637 3793 1743
Size: 78 816 1156 3724
Fragments arranged by size:
3724 1156 816 78
FspI TGC'GCA
Cuts at: 3555 3555
Size: 5774
HaeII r_GCGC'y
Cuts at: 135 163 673 768 2736 3208 3456 4478 5457
Size: 28 510 95 1968 472 248 1022 979
Cuts at: 5457 135
Size: 452
Fragments arranged by size:
1968 1022 979 510 472 452 248 95
28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 838 870 1171 1203 1288 1320 1714 1746 2206
Size: 32 301 32 85 32 394 32 460
Cuts at: 2206 2228 2238 2260 3616 3646 3648 3678 838
Size: 22 10 22 1356 30 2 30 2934
Fragments arranged by size:
2934 1356 460 394 301 85 32 32
32 32 30 30 22 22 10 2
HincII GTy'rAC
Cuts at: 1743 2813 1743
Size: 1070 4704
HpaI GTT'AAC
Cuts at: 1743 1743
Size: 5774
KasI G'GCGC_C
Cuts at: 131 3204 3452 131
Size: 3073 248 2453
Fragments arranged by size:
3073 2453 248
KpnI G_GTAC'C
Cuts at: 2794 2794
Size: 5774
MluI A'CGCG_T
Cuts at: 5207 5207
Size: 5774
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4445 4629 4445
Size: 184 5590
MscI TGG'CCA
Cuts at: 3535 3535
Size: 5774
MslI CAynn'nnrTG
Cuts at: 940 1178 2749 3763 3890 940
Size: 238 1571 1014 127 2824
Fragments arranged by size:
2824 1571 1014 238 127
MspA1I CmG'CkG
Cuts at: 150 369 654 692 970 1315 1569 1695 1706
Size: 219 285 38 278 345 254 126 11
Cuts at: 1706 2087 2885 3199 3559 4572 4817 150
Size: 381 798 314 360 1013 245 1107
Fragments arranged by size:
1107 1013 798 381 360 345 314 285
278 254 245 219 126 38 11
NaeI GCC'GGC
Cuts at: 3955 3955
Size: 5774
NarI GG'CG_CC
Cuts at: 132 3205 3453 132
Size: 3073 248 2453
Fragments arranged by size:
3073 2453 248
NcoI C'CATG_G
Cuts at: 125 2515 125
Size: 2390 3384
NgoMIV G'CCGG_C
Cuts at: 3953 3953
Size: 5774
NsiI A_TGCA'T
Cuts at: 1992 1992
Size: 5774
NspI r_CATG'y
Cuts at: 1412 2827 3327 4234 1412
Size: 1415 500 907 2952
Fragments arranged by size:
2952 1415 907 500
PciI A'CATG_T
Cuts at: 4230 4230
Size: 5774
PflMI CCAn_nnn'nTGG
Cuts at: 1200 1200
Size: 5774
PfoI T'CCnGG_A
Cuts at: 2398 2398
Size: 5774
PmeI GTTT'AAAC
Cuts at: 2773 2773
Size: 5774
PmlI CAC'GTG
Cuts at: 564 1248 564
Size: 684 5090
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 340 372 721 753 2020 2052 4913 4945 340
Size: 32 349 32 1267 32 2861 32 1169
Fragments arranged by size:
2861 1267 1169 349 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1374 2185 1374
Size: 811 4963
PshAI GACnn'nnGTC
Cuts at: 2062 2062
Size: 5774
PsiI TTA'TAA
Cuts at: 2581 2581
Size: 5774
PspOMI G'GGCC_C
Cuts at: 567 1571 567
Size: 1004 4770
PspXI vC'TCGA_Gb
Cuts at: 141 141
Size: 5774
PstI C_TGCA'G
Cuts at: 389 2686 2821 3506 389
Size: 2297 135 685 2657
Fragments arranged by size:
2657 2297 685 135
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5774
PvuII CAG'CTG
Cuts at: 369 970 1315 1706 3199 3559 369
Size: 601 345 391 1493 360 2584
Fragments arranged by size:
2584 1493 601 391 360 345
RsrII CG'GwC_CG
Cuts at: 3969 3969
Size: 5774
SacI G_AGCT'C
Cuts at: 437 2788 437
Size: 2351 3423
SacII CC_GC'GG
Cuts at: 693 693
Size: 5774
SalI G'TCGA_C
Cuts at: 2811 2811
Size: 5774
SapI GCTCTTCn'nnn_
Cuts at: 2407 3797 4007 2407
Size: 1390 210 4174
Fragments arranged by size:
4174 1390 210
SbfI CC_TGCA'GG
Cuts at: 2821 2821
Size: 5774
ScaI AGT'ACT
Cuts at: 5291 5291
Size: 5774
SexAI A'CCwGG_T
Cuts at: 1349 1349
Size: 5774
SfcI C'TryA_G
Cuts at: 33 385 858 1470 1640 2682 2817 3502 4495
Size: 352 473 612 170 1042 135 685 993
Cuts at: 4495 4686 33
Size: 191 1121
Fragments arranged by size:
1121 1042 993 685 612 473 352 191
170 135
SfoI GGC'GCC
Cuts at: 133 3206 3454 133
Size: 3073 248 2453
Fragments arranged by size:
3073 2453 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5774
SmaI CCC'GGG
Cuts at: 746 832 1380 2796 746
Size: 86 548 1416 3724
Fragments arranged by size:
3724 1416 548 86
SmlI C'TyrA_G
Cuts at: 141 2929 3844 4336 4598 141
Size: 2788 915 492 262 1317
Fragments arranged by size:
2788 1317 915 492 262
SnaBI TAC'GTA
Cuts at: 2766 2766
Size: 5774
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5774
SphI G_CATG'C
Cuts at: 1412 2827 3327 1412
Size: 1415 500 3859
Fragments arranged by size:
3859 1415 500
SspI AAT'ATT
Cuts at: 1857 1857
Size: 5774
StyI C'CwwG_G
Cuts at: 125 465 1370 2515 2908 3262 125
Size: 340 905 1145 393 354 2637
Fragments arranged by size:
2637 1145 905 393 354 340
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1052 1312 1698 2508 2626 3357 3490 3949 4158
Size: 260 386 810 118 731 133 459 209
Cuts at: 4158 1052
Size: 2668
Fragments arranged by size:
2668 810 731 459 386 260 209 133
118
TatI w'GTAC_w
Cuts at: 1934 5289 1934
Size: 3355 2419
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5291 5323 5291
Size: 32 5742
Tth111I GACn'n_nGTC
Cuts at: 1068 3571 1068
Size: 2503 3271
XbaI T'CTAG_A
Cuts at: 2805 2805
Size: 5774
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 213 426 2537 3269 132
Size: 81 213 2111 732 2637
Fragments arranged by size:
2637 2111 732 213 81
XhoI C'TCGA_G
Cuts at: 141 141
Size: 5774
XmaI C'CCGG_G
Cuts at: 744 830 1378 2794 744
Size: 86 548 1416 3724
Fragments arranged by size:
3724 1416 548 86
XmnI GAAnn'nnTTC
Cuts at: 2608 2608
Size: 5774
ZraI GAC'GTC
Cuts at: 1050 1050
Size: 5774
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AhdI AlfI AscI AvrII BglI BmtI
BsiWI BsmBI BsrGI BstZ17I ClaI EcoRV FseI FspAI
HindIII MfeI NdeI NheI NotI NruI PacI PasI
PsrI SanDI SfiI SgrAI SrfI StuI SwaI