Restriction map for
pF1KSDA1437
(Circular) (Six-base) MAPSORT of: pF1KSDA1437.seq Check: 4928 from: 1 to: 5567
pF1KSDA1437 5567 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1838 2045 2059 1838
Size: 207 14 5346
Fragments arranged by size:
5346 207 14
AatII G_ACGT'C
Cuts at: 1229 1229
Size: 5567
AccI GT'mk_AC
Cuts at: 974 1138 2605 974
Size: 164 1467 3936
Fragments arranged by size:
3936 1467 164
Acc65I G'GTAC_C
Cuts at: 2583 2583
Size: 5567
AclI AA'CG_TT
Cuts at: 5407 5407
Size: 5567
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 628 669 1690 1941 3392 3824 4571 4839
Size: 423 41 1021 251 1451 432 747 268
Cuts at: 4839 205
Size: 933
Fragments arranged by size:
1451 1021 933 747 432 423 268 251
41
AfeI AGC'GCT
Cuts at: 1291 1291
Size: 5567
AflII C'TTAA_G
Cuts at: 2056 2056
Size: 5567
AflIII A'CryG_T
Cuts at: 2919 4023 5000 2919
Size: 1104 977 3486
Fragments arranged by size:
3486 1104 977
AgeI A'CCGG_T
Cuts at: 3955 3955
Size: 5567
AhdI GACnn_n'nnGTC
Cuts at: 714 714
Size: 5567
AleI CACnn'nnGTG
Cuts at: 1146 1816 1146
Size: 670 4897
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 503 2365 503
Size: 1862 3705
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 114 146 2479 2511 114
Size: 32 2333 32 3170
Fragments arranged by size:
3170 2333 32 32
AlwNI CAG_nnn'CTG
Cuts at: 349 1839 2037 2361 2872 4439 349
Size: 1490 198 324 511 1567 1477
Fragments arranged by size:
1567 1490 1477 511 324 198
ApaI G_GGCC'C
Cuts at: 406 642 2331 406
Size: 236 1689 3642
Fragments arranged by size:
3642 1689 236
ApaLI G'TGCA_C
Cuts at: 982 1825 4337 982
Size: 843 2512 2212
Fragments arranged by size:
2512 2212 843
ApoI r'AATT_y
Cuts at: 559 2571 4920 5176 559
Size: 2012 2349 256 950
Fragments arranged by size:
2349 2012 950 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5567
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5567
AvaI C'yCGr_G
Cuts at: 2512 2587 2512
Size: 75 5492
BamHI G'GATC_C
Cuts at: 179 2592 179
Size: 2413 3154
BanI G'GyrC_C
Cuts at: 434 1858 2583 2997 3245 3280 434
Size: 1424 725 414 248 35 2721
Fragments arranged by size:
2721 1424 725 414 248 35
BanII G_rGCy'C
Cuts at: 146 362 406 642 1754 2306 2331 2581 3611
Size: 216 44 236 1112 552 25 250 1030
Cuts at: 3611 146
Size: 2102
Fragments arranged by size:
2102 1112 1030 552 250 236 216 44
25
BbeI G_GCGC'C
Cuts at: 3001 3249 3001
Size: 248 5319
BbsI GAAGACnn'nnnn_
Cuts at: 237 1293 1678 237
Size: 1056 385 4126
Fragments arranged by size:
4126 1056 385
BbvCI CC'TCA_GC
Cuts at: 2200 2200
Size: 5567
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2127 2161 2127
Size: 34 5533
BciVI GTATCCnnnnn_n'
Cuts at: 3465 4226 5567 3465
Size: 761 1341 3465
Fragments arranged by size:
3465 1341 761
BclI T'GATC_A
Cuts at: 941 3087 941
Size: 2146 3421
BglI GCCn_nnn'nGGC
Cuts at: 410 1034 2272 410
Size: 624 1238 3705
Fragments arranged by size:
3705 1238 624
BglII A'GATC_T
Cuts at: 3082 5119 3082
Size: 2037 3530
BlpI GC'TnA_GC
Cuts at: 2679 2679
Size: 5567
Bme1580I G_kGCm'C
Cuts at: 406 642 755 986 1059 1829 1861 2331 2455
Size: 236 113 231 73 770 32 470 124
Cuts at: 2455 3192 3285 4341 406
Size: 737 93 1056 1632
Fragments arranged by size:
1632 1056 770 737 470 236 231 124
113 93 73 32
BmgBI CAC'GTC
Cuts at: 738 1002 1500 738
Size: 264 498 4805
Fragments arranged by size:
4805 498 264
BmrI ACTGGGnnnn_n'
Cuts at: 2779 2860 3195 5339 2779
Size: 81 335 2144 3007
Fragments arranged by size:
3007 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3934 3934
Size: 5567
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 1429 1489 1504 1699 1933 1954 2137 2239
Size: 825 60 15 195 234 21 183 102
Cuts at: 2239 2352 2443 604
Size: 113 91 3728
Fragments arranged by size:
3728 825 234 195 183 113 102 91
60 21 15
Bpu10I CC'TnA_GC
Cuts at: 1705 1789 2200 1705
Size: 84 411 5072
Fragments arranged by size:
5072 411 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1153 1462 1522 1747 1762 1870 1972 1987 2446
Size: 309 60 225 15 108 102 15 459
Cuts at: 2446 2743 3622 4114 4412 1153
Size: 297 879 492 298 2308
Fragments arranged by size:
2308 879 492 459 309 298 297 225
108 102 60 15 15
BsaI GGTCTCn'nnnn_
Cuts at: 5271 5271
Size: 5567
BsaAI yAC'GTr
Cuts at: 450 981 2559 2920 450
Size: 531 1578 361 3097
Fragments arranged by size:
3097 1578 531 361
BsaBI GATnn'nnATC
Cuts at: 6 3081 5118 6
Size: 3075 2037 455
Fragments arranged by size:
3075 2037 455
BsaHI Gr'CG_yC
Cuts at: 1090 1226 2998 3246 3948 5444 1090
Size: 136 1772 248 702 1496 1213
Fragments arranged by size:
1772 1496 1213 702 248 136
BsaWI w'CCGG_w
Cuts at: 132 176 875 2279 2298 2756 3277 3955 4229
Size: 44 699 1404 19 458 521 678 274
Cuts at: 4229 4376 4787 132
Size: 147 411 912
Fragments arranged by size:
1404 912 699 678 521 458 411 274
147 44 19
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 358 388 2280 2310 2479 2509 2515 2545 358
Size: 30 1892 30 169 30 6 30 3380
Fragments arranged by size:
3380 1892 169 30 30 30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 676 904 1213 1696 2029 2500 676
Size: 228 309 483 333 471 3743
Fragments arranged by size:
3743 483 471 333 309 228
BseYI C'CCAG_C
Cuts at: 338 1513 1523 2162 2442 2992 3750 4327 4823
Size: 1175 10 639 280 550 758 577 496
Cuts at: 4823 338
Size: 1082
Fragments arranged by size:
1175 1082 758 639 577 550 496 280
10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 502 993 1237 1399 1603 1672 2023 2317
Size: 39 491 244 162 204 69 351 294
Cuts at: 2317 463
Size: 3713
Fragments arranged by size:
3713 491 351 294 244 204 162 69
39
BsiEI CG_ry'CG
Cuts at: 104 725 3109 3155 4363 104
Size: 621 2384 46 1208 1308
Fragments arranged by size:
2384 1308 1208 621 46
BsiHKAI G_wGCw'C
Cuts at: 146 590 986 1754 1829 2306 2581 3359 3549
Size: 444 396 768 75 477 275 778 190
Cuts at: 3549 4341 146
Size: 792 1372
Fragments arranged by size:
1372 792 778 768 477 444 396 275
190 75
BsmBI CGTCTCn'nnnn_
Cuts at: 802 2281 802
Size: 1479 4088
Bsp1286I G_dGCh'C
Cuts at: 146 362 406 590 642 755 986 1059 1754
Size: 216 44 184 52 113 231 73 695
Cuts at: 1754 1829 1861 2306 2331 2455 2581 3192 3285
Size: 75 32 445 25 124 126 611 93
Cuts at: 3285 3359 3549 3611 4341 146
Size: 74 190 62 730 1372
Fragments arranged by size:
1372 730 695 611 445 231 216 190
184 126 124 113 93 75 74 73
62 52 44 32 25
BspEI T'CCGG_A
Cuts at: 875 2298 4787 875
Size: 1423 2489 1655
Fragments arranged by size:
2489 1655 1423
BspHI T'CATG_A
Cuts at: 4743 4743
Size: 5567
BspMI ACCTGCnnnn'nnnn_
Cuts at: 252 1616 1838 1979 2045 2059 2617 2831 3133
Size: 1364 222 141 66 14 558 214 302
Cuts at: 3133 3514 252
Size: 381 2305
Fragments arranged by size:
2305 1364 558 381 302 222 214 141
66 14
BsrBI CCG'CTC
Cuts at: 50 803 1278 2526 3859 3913 5397 5562 50
Size: 753 475 1248 1333 54 1484 165 55
Fragments arranged by size:
1484 1333 1248 753 475 165 55 54
BsrDI GCAATG_nn'
Cuts at: 327 1517 3479 327
Size: 1190 1962 2415
Fragments arranged by size:
2415 1962 1190
BsrFI r'CCGG_y
Cuts at: 431 678 2417 3565 3746 3955 431
Size: 247 1739 1148 181 209 2043
Fragments arranged by size:
2043 1739 1148 247 209 181
BssSI C'ACGA_G
Cuts at: 633 3838 4196 633
Size: 3205 358 2004
Fragments arranged by size:
3205 2004 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2361 2944 2361
Size: 583 4984
BstBI TT'CG_AA
Cuts at: 110 3928 110
Size: 3818 1749
BstEII G'GTnAC_C
Cuts at: 264 303 264
Size: 39 5528
BstYI r'GATC_y
Cuts at: 1 179 1082 1646 1658 2153 2592 3059 3082
Size: 178 903 564 12 495 439 467 23
Cuts at: 3082 3417 3663 4675 4761 4773 5119 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 903 564 495 467 449 439 346
335 246 178 86 23 12 12
BstZ17I GTA'TAC
Cuts at: 1139 1139
Size: 5567
BtgI C'CryG_G
Cuts at: 190 704 1013 190
Size: 514 309 4744
Fragments arranged by size:
4744 514 309
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2061 3517 3698 3861 2061
Size: 1456 181 163 3767
Fragments arranged by size:
3767 1456 181 163
BtsI GCAGTG_nn'
Cuts at: 2359 2455 2503 2810 2359
Size: 96 48 307 5116
Fragments arranged by size:
5116 307 96 48
DraI TTT'AAA
Cuts at: 2566 2566
Size: 5567
DraIII CAC_nnn'GTG
Cuts at: 594 594
Size: 5567
DrdI GACnn_nn'nnGTC
Cuts at: 3273 4131 3273
Size: 858 4709
EaeI y'GGCC_r
Cuts at: 1067 1565 2273 3152 3326 3717 3744 5089 1067
Size: 498 708 879 174 391 27 1345 1545
Fragments arranged by size:
1545 1345 879 708 498 391 174 27
EagI C'GGCC_G
Cuts at: 3152 3152
Size: 5567
EarI CTCTTCn'nnn_
Cuts at: 1081 2173 2311 3590 3800 1081
Size: 1092 138 1279 210 2848
Fragments arranged by size:
2848 1279 1092 210 138
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3775 4085 4231 5377 3775
Size: 310 146 1146 3965
Fragments arranged by size:
3965 1146 310 146
EcoICRI GAG'CTC
Cuts at: 144 1752 2304 2579 144
Size: 1608 552 275 3132
Fragments arranged by size:
3132 1608 552 275
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 604 628 669 1429 1489 1504 1690 1699
Size: 399 24 41 760 60 15 186 9
Cuts at: 1699 1933 1941 1954 2137 2239 2352 2443 3392
Size: 234 8 13 183 102 113 91 949
Cuts at: 3392 3824 4571 4839 205
Size: 432 747 268 933
Fragments arranged by size:
949 933 760 747 432 399 268 234
186 183 113 102 91 60 41 24
15 13 9 8
EcoNI CCTnn'n_nnAGG
Cuts at: 1309 1309
Size: 5567
EcoO109I rG'GnC_Cy
Cuts at: 403 638 639 1473 2328 2490 2706 403
Size: 235 1 834 855 162 216 3264
Fragments arranged by size:
3264 855 834 235 216 162 1
EcoRI G'AATT_C
Cuts at: 2571 2571
Size: 5567
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 699 1254 3586 699
Size: 555 2332 2680
Fragments arranged by size:
2680 2332 555
FspI TGC'GCA
Cuts at: 3348 3348
Size: 5567
HaeII r_GCGC'y
Cuts at: 853 1164 1293 1582 1618 2124 3001 3249 4271
Size: 311 129 289 36 506 877 248 1022
Cuts at: 4271 5250 853
Size: 979 1170
Fragments arranged by size:
1170 1022 979 877 506 311 289 248
129 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 202 234 700 732 2280 2312 3409 3439 3441
Size: 32 466 32 1548 32 1097 30 2
Cuts at: 3441 3471 202
Size: 30 2298
Fragments arranged by size:
2298 1548 1097 466 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2606 2606
Size: 5567
KasI G'GCGC_C
Cuts at: 2997 3245 2997
Size: 248 5319
KpnI G_GTAC'C
Cuts at: 2587 2587
Size: 5567
MluI A'CGCG_T
Cuts at: 5000 5000
Size: 5567
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 626 1289 4238 4422 626
Size: 663 2949 184 1771
Fragments arranged by size:
2949 1771 663 184
MscI TGG'CCA
Cuts at: 1069 3328 1069
Size: 2259 3308
MslI CAynn'nnrTG
Cuts at: 232 937 1132 1146 1816 3556 3683 232
Size: 705 195 14 670 1740 127 2116
Fragments arranged by size:
2116 1740 705 670 195 127 14
MspA1I CmG'CkG
Cuts at: 765 1052 1338 1380 1564 1653 1925 2471 2678
Size: 287 286 42 184 89 272 546 207
Cuts at: 2678 2992 3352 4365 4610 765
Size: 314 360 1013 245 1722
Fragments arranged by size:
1722 1013 546 360 314 287 286 272
245 207 184 89 42
NaeI GCC'GGC
Cuts at: 680 3748 680
Size: 3068 2499
NarI GG'CG_CC
Cuts at: 2998 3246 2998
Size: 248 5319
NcoI C'CATG_G
Cuts at: 704 704
Size: 5567
NgoMIV G'CCGG_C
Cuts at: 678 3746 678
Size: 3068 2499
NspI r_CATG'y
Cuts at: 921 1137 2620 3120 4027 921
Size: 216 1483 500 907 2461
Fragments arranged by size:
2461 1483 907 500 216
PciI A'CATG_T
Cuts at: 4023 4023
Size: 5567
PflMI CCAn_nnn'nTGG
Cuts at: 1065 1065
Size: 5567
PmeI GTTT'AAAC
Cuts at: 2566 2566
Size: 5567
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2479 2511 4706 4738 2479
Size: 32 2195 32 3308
Fragments arranged by size:
3308 2195 32 32
PpuMI rG'GwC_Cy
Cuts at: 1473 2490 1473
Size: 1017 4550
PspOMI G'GGCC_C
Cuts at: 402 638 2327 402
Size: 236 1689 3642
Fragments arranged by size:
3642 1689 236
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1708 1740 1708
Size: 32 5535
PstI C_TGCA'G
Cuts at: 263 544 764 1835 1976 2249 2357 2614 3299
Size: 281 220 1071 141 273 108 257 685
Cuts at: 3299 263
Size: 2531
Fragments arranged by size:
2531 1071 685 281 273 257 220 141
108
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5567
PvuII CAG'CTG
Cuts at: 1338 2992 3352 1338
Size: 1654 360 3553
Fragments arranged by size:
3553 1654 360
RsrII CG'GwC_CG
Cuts at: 3762 3762
Size: 5567
SacI G_AGCT'C
Cuts at: 146 1754 2306 2581 146
Size: 1608 552 275 3132
Fragments arranged by size:
3132 1608 552 275
SalI G'TCGA_C
Cuts at: 2604 2604
Size: 5567
SapI GCTCTTCn'nnn_
Cuts at: 2173 2311 3590 3800 2173
Size: 138 1279 210 3940
Fragments arranged by size:
3940 1279 210 138
SbfI CC_TGCA'GG
Cuts at: 1976 2614 1976
Size: 638 4929
ScaI AGT'ACT
Cuts at: 496 1177 5084 496
Size: 681 3907 979
Fragments arranged by size:
3907 979 681
SexAI A'CCwGG_T
Cuts at: 506 1457 506
Size: 951 4616
SfcI C'TryA_G
Cuts at: 33 259 540 760 1209 1662 1831 1972 2245
Size: 226 281 220 449 453 169 141 273
Cuts at: 2245 2353 2610 3295 4288 4479 33
Size: 108 257 685 993 191 1121
Fragments arranged by size:
1121 993 685 453 449 281 273 257
226 220 191 169 141 108
SfoI GGC'GCC
Cuts at: 2999 3247 2999
Size: 248 5319
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5567
SmaI CCC'GGG
Cuts at: 2589 2589
Size: 5567
SmlI C'TyrA_G
Cuts at: 1168 1477 1537 1762 1777 1885 1987 2002 2056
Size: 309 60 225 15 108 102 15 54
Cuts at: 2056 2461 2722 3637 4129 4391 1168
Size: 405 261 915 492 262 2344
Fragments arranged by size:
2344 915 492 405 309 262 261 225
108 102 60 54 15 15
SnaBI TAC'GTA
Cuts at: 2559 2559
Size: 5567
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5567
SphI G_CATG'C
Cuts at: 2620 3120 2620
Size: 500 5067
StuI AGG'CCT
Cuts at: 2554 2554
Size: 5567
StyI C'CwwG_G
Cuts at: 308 704 845 2701 3055 308
Size: 396 141 1856 354 2820
Fragments arranged by size:
2820 1856 396 354 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 283 2176 3150 3283 3742 3951 283
Size: 1893 974 133 459 209 1899
Fragments arranged by size:
1899 1893 974 459 209 133
TatI w'GTAC_w
Cuts at: 440 494 1175 5082 440
Size: 54 681 3907 925
Fragments arranged by size:
3907 925 681 54
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 445 477 2515 2547 5084 5116 445
Size: 32 2038 32 2537 32 896
Fragments arranged by size:
2537 2038 896 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 771 3364 771
Size: 2593 2974
XbaI T'CTAG_A
Cuts at: 2598 2598
Size: 5567
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 579 3062 579
Size: 2483 3084
XmaI C'CCGG_G
Cuts at: 2587 2587
Size: 5567
XmnI GAAnn'nnTTC
Cuts at: 613 871 1237 613
Size: 258 366 4943
Fragments arranged by size:
4943 366 258
ZraI GAC'GTC
Cuts at: 1227 1227
Size: 5567
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
BstZ17I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PpuMI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BmtI BsiWI BsmI BsrGI
BssHII BstXI Bsu36I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PshAI PsiI PspXI SacII
SanDI SfiI SgrAI SrfI SspI SwaI XhoI