Restriction map for
pF1KSDA1438
(Circular) (Six-base) MAPSORT of: pF1KSDA1438.seq Check: 4084 from: 1 to: 5930
pF1KSDA1438 5930 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 2968 2968
Size: 5930
Acc65I G'GTAC_C
Cuts at: 2946 2946
Size: 5930
AclI AA'CG_TT
Cuts at: 5770 5770
Size: 5930
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 328 430 832 1210 1222 2308 3755 4187
Size: 6 102 402 378 12 1086 1447 432
Cuts at: 4187 4934 5202 322
Size: 747 268 1050
Fragments arranged by size:
1447 1086 1050 747 432 402 378 268
102 12 6
AfeI AGC'GCT
Cuts at: 1504 1504
Size: 5930
AflIII A'CryG_T
Cuts at: 3282 4386 5363 3282
Size: 1104 977 3849
Fragments arranged by size:
3849 1104 977
AgeI A'CCGG_T
Cuts at: 4318 4318
Size: 5930
AleI CACnn'nnGTG
Cuts at: 1846 1846
Size: 5930
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2611 2611
Size: 5930
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 201 233 1852 1884 201
Size: 32 1619 32 4247
Fragments arranged by size:
4247 1619 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1200 1704 1764 1896 2218 2667 3235 4802 1200
Size: 504 60 132 322 449 568 1567 2328
Fragments arranged by size:
2328 1567 568 504 449 322 132 60
ApaI G_GGCC'C
Cuts at: 1100 1633 1903 1979 2065 1100
Size: 533 270 76 86 4965
Fragments arranged by size:
4965 533 270 86 76
ApaLI G'TGCA_C
Cuts at: 4700 4700
Size: 5930
ApoI r'AATT_y
Cuts at: 2405 2934 5283 5539 2405
Size: 529 2349 256 2796
Fragments arranged by size:
2796 2349 529 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5930
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5930
AvaI C'yCGr_G
Cuts at: 499 554 1083 1224 1540 1634 1971 2020 2950
Size: 55 529 141 316 94 337 49 930
Cuts at: 2950 499
Size: 3479
Fragments arranged by size:
3479 930 529 337 316 141 94 55
49
BamHI G'GATC_C
Cuts at: 2955 2955
Size: 5930
BanI G'GyrC_C
Cuts at: 879 1087 1228 1840 2946 3360 3608 3643 879
Size: 208 141 612 1106 414 248 35 3166
Fragments arranged by size:
3166 1106 612 414 248 208 141 35
BanII G_rGCy'C
Cuts at: 1100 1152 1296 1471 1633 1660 1809 1903 1979
Size: 52 144 175 162 27 149 94 76
Cuts at: 1979 2021 2065 2182 2516 2633 2741 2944 3974
Size: 42 44 117 334 117 108 203 1030
Cuts at: 3974 1100
Size: 3056
Fragments arranged by size:
3056 1030 334 203 175 162 149 144
117 117 108 94 76 52 44 42
27
BbeI G_GCGC'C
Cuts at: 1091 3364 3612 1091
Size: 2273 248 3409
Fragments arranged by size:
3409 2273 248
BbsI GAAGACnn'nnnn_
Cuts at: 2470 2470
Size: 5930
BbvCI CC'TCA_GC
Cuts at: 1412 2839 1412
Size: 1427 4503
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 490 524 490
Size: 34 5896
BciVI GTATCCnnnnn_n'
Cuts at: 3828 4589 5930 3828
Size: 761 1341 3828
Fragments arranged by size:
3828 1341 761
BclI T'GATC_A
Cuts at: 3450 3450
Size: 5930
BglI GCCn_nnn'nGGC
Cuts at: 1104 1156 1300 1390 1432 1104
Size: 52 144 90 42 5602
Fragments arranged by size:
5602 144 90 52 42
BglII A'GATC_T
Cuts at: 3445 5482 3445
Size: 2037 3893
BlpI GC'TnA_GC
Cuts at: 275 1369 1513 1882 2510 2668 2824 3042 275
Size: 1094 144 369 628 158 156 218 3163
Fragments arranged by size:
3163 1094 628 369 218 158 156 144
Bme1580I G_kGCm'C
Cuts at: 882 1100 1231 1633 1903 1979 2065 3555 3648
Size: 218 131 402 270 76 86 1490 93
Cuts at: 3648 4704 882
Size: 1056 2108
Fragments arranged by size:
2108 1490 1056 402 270 218 131 93
86 76
BmgBI CAC'GTC
Cuts at: 1114 1114
Size: 5930
BmrI ACTGGGnnnn_n'
Cuts at: 1039 2034 2913 3142 3223 3558 5702 1039
Size: 995 879 229 81 335 2144 1267
Fragments arranged by size:
2144 1267 995 879 335 229 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4297 4297
Size: 5930
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 568 919 1062 1391 1810 1854 2195 2263
Size: 297 351 143 329 419 44 341 68
Cuts at: 2263 2522 2587 2599 2818 271
Size: 259 65 12 219 3383
Fragments arranged by size:
3383 419 351 341 329 297 259 219
143 68 65 44 12
Bpu10I CC'TnA_GC
Cuts at: 1412 1654 2015 2735 2839 1412
Size: 242 361 720 104 4503
Fragments arranged by size:
4503 720 361 242 104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 817 1762 2340 3106 3985 4477 4775 817
Size: 945 578 766 879 492 298 1972
Fragments arranged by size:
1972 945 879 766 578 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 259 5634 259
Size: 5375 555
BsaAI yAC'GTr
Cuts at: 2922 3283 2922
Size: 361 5569
BsaBI GATnn'nnATC
Cuts at: 6 3444 5481 6
Size: 3438 2037 455
Fragments arranged by size:
3438 2037 455
BsaHI Gr'CG_yC
Cuts at: 1088 2562 3361 3609 4311 5807 1088
Size: 1474 799 248 702 1496 1211
Fragments arranged by size:
1496 1474 1211 799 702 248
BsaWI w'CCGG_w
Cuts at: 3119 3640 4318 4592 4739 5150 3119
Size: 521 678 274 147 411 3899
Fragments arranged by size:
3899 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1189 1219 1852 1882 2537 2567 2791 2821 1189
Size: 30 633 30 655 30 224 30 4298
Fragments arranged by size:
4298 655 633 224 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 656 1639 2525 656
Size: 983 886 4061
Fragments arranged by size:
4061 983 886
BseYI C'CCAG_C
Cuts at: 694 1356 1808 1921 1935 2065 2183 2198 2740
Size: 662 452 113 14 130 118 15 542
Cuts at: 2740 2854 3355 4113 4690 5186 694
Size: 114 501 758 577 496 1438
Fragments arranged by size:
1438 758 662 577 542 501 496 452
130 118 114 113 15 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1309 2884 1309
Size: 1575 4355
BsiEI CG_ry'CG
Cuts at: 104 226 3472 3518 4726 104
Size: 122 3246 46 1208 1308
Fragments arranged by size:
3246 1308 1208 122 46
BsiHKAI G_wGCw'C
Cuts at: 1375 2249 2516 2944 3722 3912 4704 1375
Size: 874 267 428 778 190 792 2601
Fragments arranged by size:
2601 874 792 778 428 267 190
BsmI GAATG_Cn'
Cuts at: 2154 2154
Size: 5930
Bsp1286I G_dGCh'C
Cuts at: 882 1100 1152 1231 1296 1375 1471 1633 1660
Size: 218 52 79 65 79 96 162 27
Cuts at: 1660 1809 1903 1979 2021 2065 2182 2249 2516
Size: 149 94 76 42 44 117 67 267
Cuts at: 2516 2633 2741 2944 3555 3648 3722 3912 3974
Size: 117 108 203 611 93 74 190 62
Cuts at: 3974 4704 882
Size: 730 2108
Fragments arranged by size:
2108 730 611 267 218 203 190 162
149 117 117 108 96 94 93 79
79 76 74 67 65 62 52 44
42 27
BspEI T'CCGG_A
Cuts at: 5150 5150
Size: 5930
BspHI T'CATG_A
Cuts at: 156 5106 156
Size: 4950 980
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2980 3194 3496 3877 2980
Size: 214 302 381 5033
Fragments arranged by size:
5033 381 302 214
BsrBI CCG'CTC
Cuts at: 50 180 1773 1906 2399 4222 4276 5760 5925
Size: 130 1593 133 493 1823 54 1484 165
Cuts at: 5925 50
Size: 55
Fragments arranged by size:
1823 1593 1484 493 165 133 130 55
54
BsrDI GCAATG_nn'
Cuts at: 3842 3842
Size: 5930
BsrFI r'CCGG_y
Cuts at: 1155 2025 3928 4109 4318 1155
Size: 870 1903 181 209 2767
Fragments arranged by size:
2767 1903 870 209 181
BssSI C'ACGA_G
Cuts at: 1615 4201 4559 1615
Size: 2586 358 2986
Fragments arranged by size:
2986 2586 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2253 3307 2253
Size: 1054 4876
BstBI TT'CG_AA
Cuts at: 110 4291 110
Size: 4181 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 372 426 2757 372
Size: 54 2331 3545
Fragments arranged by size:
3545 2331 54
BstYI r'GATC_y
Cuts at: 1 908 944 1610 2955 3422 3445 3780 4026
Size: 907 36 666 1345 467 23 335 246
Cuts at: 4026 5038 5124 5136 5482 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1345 1012 907 666 467 449 346 335
246 86 36 23 12
Bsu36I CC'TnA_GG
Cuts at: 2057 2057
Size: 5930
BtgI C'CryG_G
Cuts at: 365 887 1427 1985 2705 2750 365
Size: 522 540 558 720 45 3545
Fragments arranged by size:
3545 720 558 540 522 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 500 1538 3880 4061 4224 500
Size: 1038 2342 181 163 2206
Fragments arranged by size:
2342 2206 1038 181 163
BtsI GCAGTG_nn'
Cuts at: 568 722 1213 1446 1507 1603 2251 2675 3173
Size: 154 491 233 61 96 648 424 498
Cuts at: 3173 568
Size: 3325
Fragments arranged by size:
3325 648 498 491 424 233 154 96
61
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 585 620 585
Size: 35 5895
DraI TTT'AAA
Cuts at: 2929 2929
Size: 5930
DrdI GACnn_nn'nnGTC
Cuts at: 3636 4494 3636
Size: 858 5072
EaeI y'GGCC_r
Cuts at: 223 1157 1424 1433 2772 2883 3515 3689 4080
Size: 934 267 9 1339 111 632 174 391
Cuts at: 4080 4107 5452 223
Size: 27 1345 701
Fragments arranged by size:
1345 1339 934 701 632 391 267 174
111 27 9
EagI C'GGCC_G
Cuts at: 223 3515 223
Size: 3292 2638
EarI CTCTTCn'nnn_
Cuts at: 257 304 468 934 2866 3953 4163 257
Size: 47 164 466 1932 1087 210 2024
Fragments arranged by size:
2024 1932 1087 466 210 164 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1687 4138 4448 4594 5740 1687
Size: 2451 310 146 1146 1877
Fragments arranged by size:
2451 1877 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2514 2942 2514
Size: 428 5502
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 322 328 430 568 832 919 1062 1210
Size: 51 6 102 138 264 87 143 148
Cuts at: 1210 1222 1391 1810 1854 2195 2263 2308 2522
Size: 12 169 419 44 341 68 45 214
Cuts at: 2522 2587 2599 2818 3755 4187 4934 5202 271
Size: 65 12 219 937 432 747 268 999
Fragments arranged by size:
999 937 747 432 419 341 268 264
219 214 169 148 143 138 102 87
68 65 51 45 44 12 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1286 1327 1618 2074 2537 1286
Size: 41 291 456 463 4679
Fragments arranged by size:
4679 463 456 291 41
EcoO109I rG'GnC_Cy
Cuts at: 1020 1037 1097 1302 1629 1630 1643 1900 1975
Size: 17 60 205 327 1 13 257 75
Cuts at: 1975 1976 2053 2061 2062 2117 2270 2477 3069
Size: 1 77 8 1 55 153 207 592
Cuts at: 3069 1020
Size: 3881
Fragments arranged by size:
3881 592 327 257 207 205 153 77
75 60 55 17 13 8 1 1
1
EcoRI G'AATT_C
Cuts at: 2934 2934
Size: 5930
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3949 3949
Size: 5930
FspI TGC'GCA
Cuts at: 3711 3711
Size: 5930
HaeII r_GCGC'y
Cuts at: 1091 1254 1506 1735 2218 3364 3612 4634 5613
Size: 163 252 229 483 1146 248 1022 979
Cuts at: 5613 1091
Size: 1408
Fragments arranged by size:
1408 1146 1022 979 483 252 248 229
163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 478 510 1516 1548 1557 1589 2416 2440 2448
Size: 32 1006 32 9 32 827 24 8
Cuts at: 2448 2472 2791 2823 3772 3802 3804 3834 478
Size: 24 319 32 949 30 2 30 2574
Fragments arranged by size:
2574 1006 949 827 319 32 32 32
32 30 30 24 24 9 8 2
HincII GTy'rAC
Cuts at: 2969 2969
Size: 5930
KasI G'GCGC_C
Cuts at: 1087 3360 3608 1087
Size: 2273 248 3409
Fragments arranged by size:
3409 2273 248
KpnI G_GTAC'C
Cuts at: 2950 2950
Size: 5930
MluI A'CGCG_T
Cuts at: 5363 5363
Size: 5930
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4601 4785 4601
Size: 184 5746
MscI TGG'CCA
Cuts at: 1426 1435 2774 2885 3691 1426
Size: 9 1339 111 806 3665
Fragments arranged by size:
3665 1339 806 111 9
MslI CAynn'nnrTG
Cuts at: 424 1177 1846 3919 4046 424
Size: 753 669 2073 127 2308
Fragments arranged by size:
2308 2073 753 669 127
MspA1I CmG'CkG
Cuts at: 300 939 1034 1360 1411 1584 1764 1788 1874
Size: 639 95 326 51 173 180 24 86
Cuts at: 1874 1881 2898 3041 3355 3715 4728 4973 300
Size: 7 1017 143 314 360 1013 245 1257
Fragments arranged by size:
1257 1017 1013 639 360 326 314 245
180 173 143 95 86 51 24 7
NaeI GCC'GGC
Cuts at: 1157 4111 1157
Size: 2954 2976
NarI GG'CG_CC
Cuts at: 1088 3361 3609 1088
Size: 2273 248 3409
Fragments arranged by size:
3409 2273 248
NcoI C'CATG_G
Cuts at: 365 887 2705 2750 365
Size: 522 1818 45 3545
Fragments arranged by size:
3545 1818 522 45
NgoMIV G'CCGG_C
Cuts at: 1155 4109 1155
Size: 2954 2976
NspI r_CATG'y
Cuts at: 1746 2983 3483 4390 1746
Size: 1237 500 907 3286
Fragments arranged by size:
3286 1237 907 500
PasI CC'CwG_GG
Cuts at: 525 1023 1093 1660 1896 525
Size: 498 70 567 236 4559
Fragments arranged by size:
4559 567 498 236 70
PciI A'CATG_T
Cuts at: 4386 4386
Size: 5930
PflMI CCAn_nnn'nTGG
Cuts at: 2367 2367
Size: 5930
PfoI T'CCnGG_A
Cuts at: 2576 2684 2576
Size: 108 5822
PmeI GTTT'AAAC
Cuts at: 2929 2929
Size: 5930
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 557 589 1111 1143 1852 1884 5069 5101 557
Size: 32 522 32 709 32 3185 32 1386
Fragments arranged by size:
3185 1386 709 522 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1037 1643 2117 2270 2477 1037
Size: 606 474 153 207 4490
Fragments arranged by size:
4490 606 474 207 153
PspOMI G'GGCC_C
Cuts at: 1096 1629 1899 1975 2061 1096
Size: 533 270 76 86 4965
Fragments arranged by size:
4965 533 270 86 76
PstI C_TGCA'G
Cuts at: 917 944 1595 1610 1709 1787 2258 2977 3662
Size: 27 651 15 99 78 471 719 685
Cuts at: 3662 917
Size: 3185
Fragments arranged by size:
3185 719 685 651 471 99 78 27
15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5930
PvuII CAG'CTG
Cuts at: 300 939 1411 1584 1764 1788 1874 1881 2898
Size: 639 472 173 180 24 86 7 1017
Cuts at: 2898 3355 3715 300
Size: 457 360 2515
Fragments arranged by size:
2515 1017 639 472 457 360 180 173
86 24 7
RsrII CG'GwC_CG
Cuts at: 4125 4125
Size: 5930
SacI G_AGCT'C
Cuts at: 2516 2944 2516
Size: 428 5502
SalI G'TCGA_C
Cuts at: 2967 2967
Size: 5930
SanDI GG'GwC_CC
Cuts at: 1037 2117 2270 2477 1037
Size: 1080 153 207 4490
Fragments arranged by size:
4490 1080 207 153
SapI GCTCTTCn'nnn_
Cuts at: 468 934 3953 4163 468
Size: 466 3019 210 2235
Fragments arranged by size:
3019 2235 466 210
SbfI CC_TGCA'GG
Cuts at: 1595 2977 1595
Size: 1382 4548
ScaI AGT'ACT
Cuts at: 5447 5447
Size: 5930
SfcI C'TryA_G
Cuts at: 33 913 940 1591 1606 1705 1783 2254 2973
Size: 880 27 651 15 99 78 471 719
Cuts at: 2973 3658 4651 4842 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 880 719 685 651 471 191
99 78 27 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 1432 1432
Size: 5930
SfoI GGC'GCC
Cuts at: 1089 3362 3610 1089
Size: 2273 248 3409
Fragments arranged by size:
3409 2273 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5930
SmaI CCC'GGG
Cuts at: 1542 1636 1973 2952 1542
Size: 94 337 979 4520
Fragments arranged by size:
4520 979 337 94
SmlI C'TyrA_G
Cuts at: 832 1777 2355 3085 4000 4492 4754 832
Size: 945 578 730 915 492 262 2008
Fragments arranged by size:
2008 945 915 730 578 492 262
SnaBI TAC'GTA
Cuts at: 2922 2922
Size: 5930
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5930
SphI G_CATG'C
Cuts at: 1746 2983 3483 1746
Size: 1237 500 4193
Fragments arranged by size:
4193 1237 500
StuI AGG'CCT
Cuts at: 357 1400 1597 357
Size: 1043 197 4690
Fragments arranged by size:
4690 1043 197
StyI C'CwwG_G
Cuts at: 365 803 887 1298 2705 2750 3064 3418 365
Size: 438 84 411 1407 45 314 354 2877
Fragments arranged by size:
2877 1407 438 411 354 314 84 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 613 1925 2264 2718 3513 3646 4105 4314 613
Size: 1312 339 454 795 133 459 209 2229
Fragments arranged by size:
2229 1312 795 459 454 339 209 133
TatI w'GTAC_w
Cuts at: 845 5445 845
Size: 4600 1330
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1595 1627 2537 2569 2893 2925 5447 5479 1595
Size: 32 910 32 324 32 2522 32 2046
Fragments arranged by size:
2522 2046 910 324 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3727 3727
Size: 5930
XbaI T'CTAG_A
Cuts at: 2961 2961
Size: 5930
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 372 1443 2748 3425 372
Size: 1071 1305 677 2877
Fragments arranged by size:
2877 1305 1071 677
XmaI C'CCGG_G
Cuts at: 1540 1634 1971 2950 1540
Size: 94 337 979 4520
Fragments arranged by size:
4520 979 337 94
XmnI GAAnn'nnTTC
Cuts at: 1864 1864
Size: 5930
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AscI AvrII BaeI BfrBI
BmtI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I ClaI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PmlI PshAI PsiI
PspXI PsrI SacII SexAI SgrAI SrfI SspI SwaI
XhoI ZraI