Restriction map for
pF1KSDA1439
(Circular) (Six-base) MAPSORT of: pF1KSDA1439.seq Check: 7122 from: 1 to: 4664
pF1KSDA1439 4664 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 1702 1702
Size: 4664
Acc65I G'GTAC_C
Cuts at: 1643 1680 1643
Size: 37 4627
AclI AA'CG_TT
Cuts at: 4504 4504
Size: 4664
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1114 2489 2921 3668 3936 1114
Size: 1375 432 747 268 1842
Fragments arranged by size:
1842 1375 747 432 268
AflIII A'CryG_T
Cuts at: 2016 3120 4097 2016
Size: 1104 977 2583
Fragments arranged by size:
2583 1104 977
AgeI A'CCGG_T
Cuts at: 3052 3052
Size: 4664
AlwNI CAG_nnn'CTG
Cuts at: 844 1969 3536 844
Size: 1125 1567 1972
Fragments arranged by size:
1972 1567 1125
ApaLI G'TGCA_C
Cuts at: 1405 3434 1405
Size: 2029 2635
ApoI r'AATT_y
Cuts at: 157 852 1318 1668 4017 4273 157
Size: 695 466 350 2349 256 548
Fragments arranged by size:
2349 695 548 466 350 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4664
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4664
AvaI C'yCGr_G
Cuts at: 1684 1684
Size: 4664
BamHI G'GATC_C
Cuts at: 1602 1689 1602
Size: 87 4577
BanI G'GyrC_C
Cuts at: 1482 1643 1680 2094 2342 2377 1482
Size: 161 37 414 248 35 3769
Fragments arranged by size:
3769 414 248 161 37 35
BanII G_rGCy'C
Cuts at: 608 946 1678 2708 608
Size: 338 732 1030 2564
Fragments arranged by size:
2564 1030 732 338
BbeI G_GCGC'C
Cuts at: 2098 2346 2098
Size: 248 4416
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1202 1236 1202
Size: 34 4630
BciVI GTATCCnnnnn_n'
Cuts at: 1645 2562 3323 4664 1645
Size: 917 761 1341 1645
Fragments arranged by size:
1645 1341 917 761
BclI T'GATC_A
Cuts at: 2184 2184
Size: 4664
BglII A'GATC_T
Cuts at: 2179 4216 2179
Size: 2037 2627
BlpI GC'TnA_GC
Cuts at: 1776 1776
Size: 4664
Bme1580I G_kGCm'C
Cuts at: 1409 2289 2382 3438 1409
Size: 880 93 1056 2635
Fragments arranged by size:
2635 1056 880 93
BmgBI CAC'GTC
Cuts at: 192 1508 1538 192
Size: 1316 30 3318
Fragments arranged by size:
3318 1316 30
BmrI ACTGGGnnnn_n'
Cuts at: 1876 1957 2292 4436 1876
Size: 81 335 2144 2104
Fragments arranged by size:
2144 2104 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3031 3031
Size: 4664
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 525 525
Size: 4664
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 943 1840 2719 3211 3509 943
Size: 897 879 492 298 2098
Fragments arranged by size:
2098 897 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4368 4368
Size: 4664
BsaAI yAC'GTr
Cuts at: 1656 2017 1656
Size: 361 4303
BsaBI GATnn'nnATC
Cuts at: 6 2178 4215 6
Size: 2172 2037 455
Fragments arranged by size:
2172 2037 455
BsaHI Gr'CG_yC
Cuts at: 2095 2343 3045 4541 2095
Size: 248 702 1496 2218
Fragments arranged by size:
2218 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1853 2374 3052 3326 3473 3884 1853
Size: 521 678 274 147 411 2633
Fragments arranged by size:
2633 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1482 1512 1482
Size: 30 4634
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 927 1009 1134 927
Size: 82 125 4457
Fragments arranged by size:
4457 125 82
BseYI C'CCAG_C
Cuts at: 706 925 1281 1558 2089 2847 3424 3920 706
Size: 219 356 277 531 758 577 496 1450
Fragments arranged by size:
1450 758 577 531 496 356 277 219
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1544 1544
Size: 4664
BsiEI CG_ry'CG
Cuts at: 104 2206 2252 3460 104
Size: 2102 46 1208 1308
Fragments arranged by size:
2102 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 946 1409 1678 2456 2646 3438 946
Size: 463 269 778 190 792 2172
Fragments arranged by size:
2172 792 778 463 269 190
BsmI GAATG_Cn'
Cuts at: 1079 1079
Size: 4664
BsmBI CGTCTCn'nnnn_
Cuts at: 1300 1300
Size: 4664
Bsp1286I G_dGCh'C
Cuts at: 608 946 1409 1678 2289 2382 2456 2646 2708
Size: 338 463 269 611 93 74 190 62
Cuts at: 2708 3438 608
Size: 730 1834
Fragments arranged by size:
1834 730 611 463 338 269 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3884 3884
Size: 4664
BspHI T'CATG_A
Cuts at: 3840 3840
Size: 4664
BspMI ACCTGCnnnn'nnnn_
Cuts at: 230 1714 1928 2230 2611 230
Size: 1484 214 302 381 2283
Fragments arranged by size:
2283 1484 381 302 214
BsrBI CCG'CTC
Cuts at: 50 138 2956 3010 4494 4659 50
Size: 88 2818 54 1484 165 55
Fragments arranged by size:
2818 1484 165 88 55 54
BsrDI GCAATG_nn'
Cuts at: 914 2576 914
Size: 1662 3002
BsrFI r'CCGG_y
Cuts at: 1479 2662 2843 3052 1479
Size: 1183 181 209 3091
Fragments arranged by size:
3091 1183 209 181
BssSI C'ACGA_G
Cuts at: 2935 3293 2935
Size: 358 4306
BstAPI GCAn_nnn'nTGC
Cuts at: 2041 2041
Size: 4664
BstBI TT'CG_AA
Cuts at: 110 3025 110
Size: 2915 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1261 1261
Size: 4664
BstYI r'GATC_y
Cuts at: 1 1602 1689 2156 2179 2514 2760 3772 3858
Size: 1601 87 467 23 335 246 1012 86
Cuts at: 3858 3870 4216 1
Size: 12 346 449
Fragments arranged by size:
1601 1012 467 449 346 335 246 87
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1301 1301
Size: 4664
BtgI C'CryG_G
Cuts at: 1597 1597
Size: 4664
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2614 2795 2958 2614
Size: 181 163 4320
Fragments arranged by size:
4320 181 163
BtsI GCAGTG_nn'
Cuts at: 1907 1907
Size: 4664
DraI TTT'AAA
Cuts at: 537 1663 537
Size: 1126 3538
DrdI GACnn_nn'nnGTC
Cuts at: 775 2370 3228 775
Size: 1595 858 2211
Fragments arranged by size:
2211 1595 858
EaeI y'GGCC_r
Cuts at: 1460 2249 2423 2814 2841 4186 1460
Size: 789 174 391 27 1345 1938
Fragments arranged by size:
1938 1345 789 391 174 27
EagI C'GGCC_G
Cuts at: 2249 2249
Size: 4664
EarI CTCTTCn'nnn_
Cuts at: 275 1232 2687 2897 275
Size: 957 1455 210 2042
Fragments arranged by size:
2042 1455 957 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 475 2872 3182 3328 4474 475
Size: 2397 310 146 1146 665
Fragments arranged by size:
2397 1146 665 310 146
EcoICRI GAG'CTC
Cuts at: 944 1676 944
Size: 732 3932
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 525 1114 2489 2921 3668 3936 525
Size: 589 1375 432 747 268 1253
Fragments arranged by size:
1375 1253 747 589 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 487 487
Size: 4664
EcoO109I rG'GnC_Cy
Cuts at: 1192 1803 1192
Size: 611 4053
EcoRI G'AATT_C
Cuts at: 1668 1668
Size: 4664
EcoRV GAT'ATC
Cuts at: 372 372
Size: 4664
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2683 2683
Size: 4664
FspI TGC'GCA
Cuts at: 2445 2445
Size: 4664
HaeII r_GCGC'y
Cuts at: 573 957 1023 2098 2346 3368 4347 573
Size: 384 66 1075 248 1022 979 890
Fragments arranged by size:
1075 1022 979 890 384 248 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2506 2536 2538 2568 2506
Size: 30 2 30 4602
Fragments arranged by size:
4602 30 30 2
HincII GTy'rAC
Cuts at: 1511 1703 1511
Size: 192 4472
HindIII A'AGCT_T
Cuts at: 1608 1608
Size: 4664
KasI G'GCGC_C
Cuts at: 2094 2342 2094
Size: 248 4416
KpnI G_GTAC'C
Cuts at: 1647 1684 1647
Size: 37 4627
MluI A'CGCG_T
Cuts at: 4097 4097
Size: 4664
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 399 467 491 639 1082 1108 1350 1525 1572
Size: 68 24 148 443 26 242 175 47
Cuts at: 1572 3335 3519 399
Size: 1763 184 1544
Fragments arranged by size:
1763 1544 443 242 184 175 148 68
47 26 24
MscI TGG'CCA
Cuts at: 1462 2425 1462
Size: 963 3701
MslI CAynn'nnrTG
Cuts at: 1429 2653 2780 1429
Size: 1224 127 3313
Fragments arranged by size:
3313 1224 127
MspA1I CmG'CkG
Cuts at: 549 1775 2089 2449 3462 3707 549
Size: 1226 314 360 1013 245 1506
Fragments arranged by size:
1506 1226 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2845 2845
Size: 4664
NarI GG'CG_CC
Cuts at: 2095 2343 2095
Size: 248 4416
NgoMIV G'CCGG_C
Cuts at: 2843 2843
Size: 4664
NspI r_CATG'y
Cuts at: 679 1213 1717 2217 3124 679
Size: 534 504 500 907 2219
Fragments arranged by size:
2219 907 534 504 500
PasI CC'CwG_GG
Cuts at: 1149 1149
Size: 4664
PciI A'CATG_T
Cuts at: 3120 3120
Size: 4664
PflMI CCAn_nnn'nTGG
Cuts at: 748 1033 748
Size: 285 4379
PfoI T'CCnGG_A
Cuts at: 764 900 764
Size: 136 4528
PmeI GTTT'AAAC
Cuts at: 1663 1663
Size: 4664
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3803 3835 3803
Size: 32 4632
PpuMI rG'GwC_Cy
Cuts at: 1192 1192
Size: 4664
PstI C_TGCA'G
Cuts at: 227 840 1711 2396 227
Size: 613 871 685 2495
Fragments arranged by size:
2495 871 685 613
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4664
PvuII CAG'CTG
Cuts at: 2089 2449 2089
Size: 360 4304
RsrII CG'GwC_CG
Cuts at: 2859 2859
Size: 4664
SacI G_AGCT'C
Cuts at: 946 1678 946
Size: 732 3932
SalI G'TCGA_C
Cuts at: 1701 1701
Size: 4664
SapI GCTCTTCn'nnn_
Cuts at: 2687 2897 2687
Size: 210 4454
SbfI CC_TGCA'GG
Cuts at: 227 1711 227
Size: 1484 3180
ScaI AGT'ACT
Cuts at: 558 4181 558
Size: 3623 1041
SfcI C'TryA_G
Cuts at: 33 223 836 888 1707 2392 3385 3576 33
Size: 190 613 52 819 685 993 191 1121
Fragments arranged by size:
1121 993 819 685 613 191 190 52
SfoI GGC'GCC
Cuts at: 2096 2344 2096
Size: 248 4416
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4664
SmaI CCC'GGG
Cuts at: 1686 1686
Size: 4664
SmlI C'TyrA_G
Cuts at: 958 1819 2734 3226 3488 958
Size: 861 915 492 262 2134
Fragments arranged by size:
2134 915 861 492 262
SnaBI TAC'GTA
Cuts at: 1656 1656
Size: 4664
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4664
SphI G_CATG'C
Cuts at: 679 1213 1717 2217 679
Size: 534 504 500 3126
Fragments arranged by size:
3126 534 504 500
StuI AGG'CCT
Cuts at: 1467 1467
Size: 4664
StyI C'CwwG_G
Cuts at: 1014 1395 1798 2152 1014
Size: 381 403 354 3526
Fragments arranged by size:
3526 403 381 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 163 1260 1295 1572 2247 2380 2839 3048 163
Size: 1097 35 277 675 133 459 209 1779
Fragments arranged by size:
1779 1097 675 459 277 209 133 35
TatI w'GTAC_w
Cuts at: 556 4179 556
Size: 3623 1041
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1235 1267 1482 1514 4181 4213 1235
Size: 32 215 32 2667 32 1686
Fragments arranged by size:
2667 1686 215 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1635 2461 1635
Size: 826 3838
XbaI T'CTAG_A
Cuts at: 1695 1695
Size: 4664
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 745 2159 745
Size: 1414 3250
XmaI C'CCGG_G
Cuts at: 1684 1684
Size: 4664
XmnI GAAnn'nnTTC
Cuts at: 179 179
Size: 4664
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI
BmtI Bpu10I BsiWI BsrGI BssHII BstEII BstZ17I ClaI
CspCI DraIII FseI FspAI HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI SspI SwaI XhoI ZraI