Restriction map for
pF1KSDA1441
(Circular) (Six-base) MAPSORT of: pF1KSDA1441.seq Check: 8981 from: 1 to: 6848
pF1KSDA1441 6848 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1200 1819 1200
Size: 619 6229
AatII G_ACGT'C
Cuts at: 2482 2482
Size: 6848
AccI GT'mk_AC
Cuts at: 3886 3886
Size: 6848
Acc65I G'GTAC_C
Cuts at: 3864 3864
Size: 6848
AclI AA'CG_TT
Cuts at: 6688 6688
Size: 6848
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 299 2268 2885 3213 4673 5105 5852 6120 299
Size: 1969 617 328 1460 432 747 268 1027
Fragments arranged by size:
1969 1460 1027 747 617 432 328 268
AfeI AGC'GCT
Cuts at: 3124 3124
Size: 6848
AflII C'TTAA_G
Cuts at: 1108 1108
Size: 6848
AflIII A'CryG_T
Cuts at: 3255 4200 5304 6281 3255
Size: 945 1104 977 3822
Fragments arranged by size:
3822 1104 977 945
AgeI A'CCGG_T
Cuts at: 5236 5236
Size: 6848
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1342 1669 2368 1342
Size: 327 699 5822
Fragments arranged by size:
5822 699 327
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 509 541 509
Size: 32 6816
AlwNI CAG_nnn'CTG
Cuts at: 298 898 1183 1564 2071 2875 4153 5720 298
Size: 600 285 381 507 804 1278 1567 1426
Fragments arranged by size:
1567 1426 1278 804 600 507 381 285
ApaI G_GGCC'C
Cuts at: 447 3291 447
Size: 2844 4004
ApaLI G'TGCA_C
Cuts at: 5618 5618
Size: 6848
ApoI r'AATT_y
Cuts at: 3852 6201 6457 3852
Size: 2349 256 4243
Fragments arranged by size:
4243 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6848
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6848
AvaI C'yCGr_G
Cuts at: 400 680 3320 3868 400
Size: 280 2640 548 3380
Fragments arranged by size:
3380 2640 548 280
BamHI G'GATC_C
Cuts at: 3873 3873
Size: 6848
BanI G'GyrC_C
Cuts at: 565 903 929 1458 1525 2420 2812 3152 3523
Size: 338 26 529 67 895 392 340 371
Cuts at: 3523 3864 4278 4526 4561 565
Size: 341 414 248 35 2852
Fragments arranged by size:
2852 895 529 414 392 371 341 340
338 248 67 35 26
BanII G_rGCy'C
Cuts at: 447 514 693 940 1684 1895 2936 3291 3342
Size: 67 179 247 744 211 1041 355 51
Cuts at: 3342 3813 3862 4892 447
Size: 471 49 1030 2403
Fragments arranged by size:
2403 1041 1030 744 471 355 247 211
179 67 51 49
BbeI G_GCGC'C
Cuts at: 3156 4282 4530 3156
Size: 1126 248 5474
Fragments arranged by size:
5474 1126 248
BbsI GAAGACnn'nnnn_
Cuts at: 145 287 1007 1830 2622 2885 3362 145
Size: 142 720 823 792 263 477 3631
Fragments arranged by size:
3631 823 792 720 477 263 142
BbvCI CC'TCA_GC
Cuts at: 1205 1223 1691 2075 1205
Size: 18 468 384 5978
Fragments arranged by size:
5978 468 384 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1782 1816 2293 2327 2383 2417 1782
Size: 34 477 34 56 34 6213
Fragments arranged by size:
6213 477 56 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2407 4746 5507 6848 2407
Size: 2339 761 1341 2407
Fragments arranged by size:
2407 2339 1341 761
BclI T'GATC_A
Cuts at: 4368 4368
Size: 6848
BfrBI ATG'CAT
Cuts at: 2304 2304
Size: 6848
BglI GCCn_nnn'nGGC
Cuts at: 480 1201 1219 1381 2227 480
Size: 721 18 162 846 5101
Fragments arranged by size:
5101 846 721 162 18
BglII A'GATC_T
Cuts at: 4363 6400 4363
Size: 2037 4811
BlpI GC'TnA_GC
Cuts at: 429 3960 429
Size: 3531 3317
Bme1580I G_kGCm'C
Cuts at: 447 568 908 932 962 1463 2248 2425 2509
Size: 121 340 24 30 501 785 177 84
Cuts at: 2509 2547 2707 2817 3291 3528 3633 4473 4566
Size: 38 160 110 474 237 105 840 93
Cuts at: 4566 5622 447
Size: 1056 1673
Fragments arranged by size:
1673 1056 840 785 501 474 340 237
177 160 121 110 105 93 84 38
30 24
BmgBI CAC'GTC
Cuts at: 2397 2397
Size: 6848
BmrI ACTGGGnnnn_n'
Cuts at: 551 886 1514 1574 1599 3285 4060 4141 4476
Size: 335 628 60 25 1686 775 81 335
Cuts at: 4476 6620 551
Size: 2144 779
Fragments arranged by size:
2144 1686 779 775 628 335 335 81
60 25
BmtI G_CTAG'C
Cuts at: 1232 1232
Size: 6848
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5215 5215
Size: 6848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 335 443 831 908 1074 1753 2173 3026
Size: 78 108 388 77 166 679 420 853
Cuts at: 3026 3464 3734 257
Size: 438 270 3371
Fragments arranged by size:
3371 853 679 438 420 388 270 166
108 78 77
Bpu10I CC'TnA_GC
Cuts at: 629 840 1205 1223 1678 1691 2075 2930 3044
Size: 211 365 18 455 13 384 855 114
Cuts at: 3044 3574 629
Size: 530 3903
Fragments arranged by size:
3903 855 530 455 384 365 211 114
18 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2740 2968 3754 4024 4903 5395 5693 2740
Size: 228 786 270 879 492 298 3895
Fragments arranged by size:
3895 879 786 492 298 270 228
BsaI GGTCTCn'nnnn_
Cuts at: 6552 6552
Size: 6848
BsaAI yAC'GTr
Cuts at: 1873 3060 3840 4201 1873
Size: 1187 780 361 4520
Fragments arranged by size:
4520 1187 780 361
BsaBI GATnn'nnATC
Cuts at: 6 1184 4362 6399 6
Size: 1178 3178 2037 455
Fragments arranged by size:
3178 2037 1178 455
BsaHI Gr'CG_yC
Cuts at: 2479 3153 4279 4527 5229 6725 2479
Size: 674 1126 248 702 1496 2602
Fragments arranged by size:
2602 1496 1126 702 674 248
BsaWI w'CCGG_w
Cuts at: 3467 4037 4558 5236 5510 5657 6068 3467
Size: 570 521 678 274 147 411 4247
Fragments arranged by size:
4247 678 570 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 509 539 687 717 2545 2575 509
Size: 30 148 30 1828 30 4782
Fragments arranged by size:
4782 1828 148 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 542 1086 2040 2638 2643 2800 2857 2899 2923
Size: 544 954 598 5 157 57 42 24
Cuts at: 2923 3403 542
Size: 480 3987
Fragments arranged by size:
3987 954 598 544 480 157 57 42
24 5
BseYI C'CCAG_C
Cuts at: 301 454 898 1497 1696 2425 2564 2572 2681
Size: 153 444 599 199 729 139 8 109
Cuts at: 2681 2794 2920 3142 3527 3639 4273 5031 5608
Size: 113 126 222 385 112 634 758 577
Cuts at: 5608 6104 301
Size: 496 1045
Fragments arranged by size:
1045 758 729 634 599 577 496 444
385 222 199 153 139 126 113 112
109 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 650 1872 2377 650
Size: 1222 505 5121
Fragments arranged by size:
5121 1222 505
BsiEI CG_ry'CG
Cuts at: 104 4390 4436 5644 104
Size: 4286 46 1208 1308
Fragments arranged by size:
4286 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1909 3862 4640 4830 5622 1909
Size: 1953 778 190 792 3135
Fragments arranged by size:
3135 1953 792 778 190
BsmI GAATG_Cn'
Cuts at: 497 497
Size: 6848
BsmBI CGTCTCn'nnnn_
Cuts at: 431 2403 3600 431
Size: 1972 1197 3679
Fragments arranged by size:
3679 1972 1197
Bsp1286I G_dGCh'C
Cuts at: 447 514 568 693 908 932 940 962 1463
Size: 67 54 125 215 24 8 22 501
Cuts at: 1463 1684 1895 1909 2248 2425 2509 2547 2707
Size: 221 211 14 339 177 84 38 160
Cuts at: 2707 2817 2936 3291 3342 3528 3633 3813 3862
Size: 110 119 355 51 186 105 180 49
Cuts at: 3862 4473 4566 4640 4830 4892 5622 447
Size: 611 93 74 190 62 730 1673
Fragments arranged by size:
1673 730 611 501 355 339 221 215
211 190 186 180 177 160 125 119
110 105 93 84 74 67 62 54
51 49 38 24 22 14 8
BspEI T'CCGG_A
Cuts at: 6068 6068
Size: 6848
BspHI T'CATG_A
Cuts at: 1919 3497 6024 1919
Size: 1578 2527 2743
Fragments arranged by size:
2743 2527 1578
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1200 1661 1819 2260 3191 3898 4112 4414 4795
Size: 461 158 441 931 707 214 302 381
Cuts at: 4795 1200
Size: 3253
Fragments arranged by size:
3253 931 707 461 441 381 302 214
158
BsrBI CCG'CTC
Cuts at: 50 2094 5140 5194 6678 6843 50
Size: 2044 3046 54 1484 165 55
Fragments arranged by size:
3046 2044 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1080 2244 4760 1080
Size: 1164 2516 3168
Fragments arranged by size:
3168 2516 1164
BsrFI r'CCGG_y
Cuts at: 1717 4846 5027 5236 1717
Size: 3129 181 209 3329
Fragments arranged by size:
3329 3129 209 181
BssSI C'ACGA_G
Cuts at: 3172 5119 5477 3172
Size: 1947 358 4543
Fragments arranged by size:
4543 1947 358
BstAPI GCAn_nnn'nTGC
Cuts at: 298 1820 2243 2676 2875 3206 3276 3749 4225
Size: 1522 423 433 199 331 70 473 476
Cuts at: 4225 298
Size: 2921
Fragments arranged by size:
2921 1522 476 473 433 423 331 199
70
BstBI TT'CG_AA
Cuts at: 110 5209 110
Size: 5099 1749
BstEII G'GTnAC_C
Cuts at: 1806 1806
Size: 6848
BstXI CCAn_nnnn'nTGG
Cuts at: 454 739 1368 1617 2773 3298 3633 454
Size: 285 629 249 1156 525 335 3669
Fragments arranged by size:
3669 1156 629 525 335 285 249
BstYI r'GATC_y
Cuts at: 1 648 1179 1634 3439 3873 4340 4363 4698
Size: 647 531 455 1805 434 467 23 335
Cuts at: 4698 4944 5956 6042 6054 6400 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1805 1012 647 531 467 455 449 434
346 335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 854 1475 2840 3148 854
Size: 621 1365 308 4554
Fragments arranged by size:
4554 1365 621 308
BtgI C'CryG_G
Cuts at: 125 339 860 2519 3450 125
Size: 214 521 1659 931 3523
Fragments arranged by size:
3523 1659 931 521 214
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4798 4979 5142 4798
Size: 181 163 6504
Fragments arranged by size:
6504 181 163
BtsI GCAGTG_nn'
Cuts at: 1818 1909 2484 3390 3538 4091 1818
Size: 91 575 906 148 553 4575
Fragments arranged by size:
4575 906 575 553 148 91
DraI TTT'AAA
Cuts at: 3847 3847
Size: 6848
DraIII CAC_nnn'GTG
Cuts at: 395 395
Size: 6848
DrdI GACnn_nn'nnGTC
Cuts at: 4554 5412 4554
Size: 858 5990
EaeI y'GGCC_r
Cuts at: 1334 2120 3300 4433 4607 4998 5025 6370 1334
Size: 786 1180 1133 174 391 27 1345 1812
Fragments arranged by size:
1812 1345 1180 1133 786 391 174 27
EagI C'GGCC_G
Cuts at: 4433 4433
Size: 6848
EarI CTCTTCn'nnn_
Cuts at: 720 1756 2900 3598 3650 4871 5081 720
Size: 1036 1144 698 52 1221 210 2487
Fragments arranged by size:
2487 1221 1144 1036 698 210 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3126 3446 5056 5366 5512 6658 3126
Size: 320 1610 310 146 1146 3316
Fragments arranged by size:
3316 1610 1146 320 310 146
EcoICRI GAG'CTC
Cuts at: 3860 3860
Size: 6848
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 299 335 443 831 908 1074 1753 2173
Size: 42 36 108 388 77 166 679 420
Cuts at: 2173 2268 2885 3026 3213 3464 3734 4673 5105
Size: 95 617 141 187 251 270 939 432
Cuts at: 5105 5852 6120 257
Size: 747 268 985
Fragments arranged by size:
985 939 747 679 617 432 420 388
270 268 251 187 166 141 108 95
77 42 36
EcoNI CCTnn'n_nnAGG
Cuts at: 632 1474 1618 632
Size: 842 144 5862
Fragments arranged by size:
5862 842 144
EcoO109I rG'GnC_Cy
Cuts at: 232 414 443 472 481 782 822 1170 1211
Size: 182 29 29 9 301 40 348 41
Cuts at: 1211 2079 3001 3287 3660 3987 232
Size: 868 922 286 373 327 3093
Fragments arranged by size:
3093 922 868 373 348 327 301 286
182 41 40 29 29 9
EcoRI G'AATT_C
Cuts at: 3852 3852
Size: 6848
EcoRV GAT'ATC
Cuts at: 1595 1595
Size: 6848
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3795 4867 3795
Size: 1072 5776
FspI TGC'GCA
Cuts at: 1798 4629 1798
Size: 2831 4017
FspAI rTGC'GCAy
Cuts at: 1798 1798
Size: 6848
HaeII r_GCGC'y
Cuts at: 2291 2781 3126 3156 4282 4530 5552 6531 2291
Size: 490 345 30 1126 248 1022 979 2608
Fragments arranged by size:
2608 1126 1022 979 490 345 248 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 687 719 1149 1181 1735 1767 2875 2907 4690
Size: 32 430 32 554 32 1108 32 1783
Cuts at: 4690 4720 4722 4752 687
Size: 30 2 30 2783
Fragments arranged by size:
2783 1783 1108 554 430 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3887 3887
Size: 6848
KasI G'GCGC_C
Cuts at: 3152 4278 4526 3152
Size: 1126 248 5474
Fragments arranged by size:
5474 1126 248
KpnI G_GTAC'C
Cuts at: 3868 3868
Size: 6848
MluI A'CGCG_T
Cuts at: 6281 6281
Size: 6848
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1768 3334 5519 5703 1768
Size: 1566 2185 184 2913
Fragments arranged by size:
2913 2185 1566 184
MscI TGG'CCA
Cuts at: 1336 4609 1336
Size: 3273 3575
MslI CAynn'nnrTG
Cuts at: 1366 1871 4837 4964 1366
Size: 505 2966 127 3250
Fragments arranged by size:
3250 2966 505 127
MspA1I CmG'CkG
Cuts at: 654 1690 1727 1971 2429 3959 4273 4633 5646
Size: 1036 37 244 458 1530 314 360 1013
Cuts at: 5646 5891 654
Size: 245 1611
Fragments arranged by size:
1611 1530 1036 1013 458 360 314 245
244 37
NaeI GCC'GGC
Cuts at: 5029 5029
Size: 6848
NarI GG'CG_CC
Cuts at: 3153 4279 4527 3153
Size: 1126 248 5474
Fragments arranged by size:
5474 1126 248
NcoI C'CATG_G
Cuts at: 125 339 2519 3450 125
Size: 214 2180 931 3523
Fragments arranged by size:
3523 2180 931 214
NgoMIV G'CCGG_C
Cuts at: 5027 5027
Size: 6848
NheI G'CTAG_C
Cuts at: 1228 1228
Size: 6848
NsiI A_TGCA'T
Cuts at: 2306 2306
Size: 6848
NspI r_CATG'y
Cuts at: 753 1797 1870 2141 2304 3259 3901 4401 5308
Size: 1044 73 271 163 955 642 500 907
Cuts at: 5308 753
Size: 2293
Fragments arranged by size:
2293 1044 955 907 642 500 271 163
73
PasI CC'CwG_GG
Cuts at: 244 2224 3342 3425 3633 244
Size: 1980 1118 83 208 3459
Fragments arranged by size:
3459 1980 1118 208 83
PciI A'CATG_T
Cuts at: 3255 5304 3255
Size: 2049 4799
PflMI CCAn_nnn'nTGG
Cuts at: 898 1947 898
Size: 1049 5799
PfoI T'CCnGG_A
Cuts at: 2378 2378
Size: 6848
PmeI GTTT'AAAC
Cuts at: 3847 3847
Size: 6848
PmlI CAC'GTG
Cuts at: 1873 1873
Size: 6848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 509 541 3479 3511 5987 6019 509
Size: 32 2938 32 2476 32 1338
Fragments arranged by size:
2938 2476 1338 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 822 1170 822
Size: 348 6500
PspOMI G'GGCC_C
Cuts at: 443 3287 443
Size: 2844 4004
PstI C_TGCA'G
Cuts at: 805 1062 2281 3202 3895 4580 805
Size: 257 1219 921 693 685 3073
Fragments arranged by size:
3073 1219 921 693 685 257
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6848
PvuII CAG'CTG
Cuts at: 1690 2429 4273 4633 1690
Size: 739 1844 360 3905
Fragments arranged by size:
3905 1844 739 360
RsrII CG'GwC_CG
Cuts at: 5043 5043
Size: 6848
SacI G_AGCT'C
Cuts at: 3862 3862
Size: 6848
SalI G'TCGA_C
Cuts at: 3885 3885
Size: 6848
SapI GCTCTTCn'nnn_
Cuts at: 1756 4871 5081 1756
Size: 3115 210 3523
Fragments arranged by size:
3523 3115 210
SbfI CC_TGCA'GG
Cuts at: 3202 3895 3202
Size: 693 6155
ScaI AGT'ACT
Cuts at: 1356 6365 1356
Size: 5009 1839
SexAI A'CCwGG_T
Cuts at: 258 258
Size: 6848
SfcI C'TryA_G
Cuts at: 33 365 801 1058 1665 1928 1979 2063 2277
Size: 332 436 257 607 263 51 84 214
Cuts at: 2277 3198 3891 4576 5569 5760 33
Size: 921 693 685 993 191 1121
Fragments arranged by size:
1121 993 921 693 685 607 436 332
263 257 214 191 84 51
SfiI GGCCn_nnn'nGGCC
Cuts at: 480 480
Size: 6848
SfoI GGC'GCC
Cuts at: 3154 4280 4528 3154
Size: 1126 248 5474
Fragments arranged by size:
5474 1126 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6848
SmaI CCC'GGG
Cuts at: 3870 3870
Size: 6848
SmlI C'TyrA_G
Cuts at: 1108 2755 2983 3769 4003 4918 5410 5672 1108
Size: 1647 228 786 234 915 492 262 2284
Fragments arranged by size:
2284 1647 915 786 492 262 234 228
SnaBI TAC'GTA
Cuts at: 3840 3840
Size: 6848
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6848
SphI G_CATG'C
Cuts at: 753 1797 1870 2304 3901 4401 753
Size: 1044 73 434 1597 500 3200
Fragments arranged by size:
3200 1597 1044 500 434 73
StuI AGG'CCT
Cuts at: 331 1104 1454 1624 1926 2386 2981 331
Size: 773 350 170 302 460 595 4198
Fragments arranged by size:
4198 773 595 460 350 302 170
StyI C'CwwG_G
Cuts at: 125 339 602 1199 2021 2519 3314 3450 3982
Size: 214 263 597 822 498 795 136 532
Cuts at: 3982 4336 125
Size: 354 2637
Fragments arranged by size:
2637 822 795 597 532 498 354 263
214 136
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1285 2779 4431 4564 5023 5232 1285
Size: 1494 1652 133 459 209 2901
Fragments arranged by size:
2901 1652 1494 459 209 133
TatI w'GTAC_w
Cuts at: 1354 1573 6363 1354
Size: 219 4790 1839
Fragments arranged by size:
4790 1839 219
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 381 413 1502 1534 2545 2577 6365 6397 381
Size: 32 1089 32 1011 32 3788 32 832
Fragments arranged by size:
3788 1089 1011 832 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1605 2626 4645 1605
Size: 1021 2019 3808
Fragments arranged by size:
3808 2019 1021
XbaI T'CTAG_A
Cuts at: 3879 3879
Size: 6848
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2433 2517 4343 2433
Size: 84 1826 4938
Fragments arranged by size:
4938 1826 84
XmaI C'CCGG_G
Cuts at: 3868 3868
Size: 6848
XmnI GAAnn'nnTTC
Cuts at: 3100 3100
Size: 6848
ZraI GAC'GTC
Cuts at: 2480 2480
Size: 6848
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AhdI AleI AscI AvrII BaeI BsiWI BsrGI BssHII
BstZ17I ClaI CspCI FseI HindIII HpaI MfeI NdeI
NotI NruI PacI PshAI PsiI PspXI PsrI SacII
SanDI SgrAI SrfI SspI SwaI XhoI