Restriction map for
pF1KSDA1442
(Circular) (Six-base) MAPSORT of: pF1KSDA1442.seq Check: 4376 from: 1 to: 4757
pF1KSDA1442 4757 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 1124 1795 1124
Size: 671 4086
Acc65I G'GTAC_C
Cuts at: 1773 1773
Size: 4757
AclI AA'CG_TT
Cuts at: 4597 4597
Size: 4757
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 733 1252 1318 1395 2582 3014 3761 4029
Size: 555 519 66 77 1187 432 747 268
Cuts at: 4029 178
Size: 906
Fragments arranged by size:
1187 906 747 555 519 432 268 77
66
AflIII A'CryG_T
Cuts at: 786 809 2109 3213 4190 786
Size: 23 1300 1104 977 1353
Fragments arranged by size:
1353 1300 1104 977 23
AgeI A'CCGG_T
Cuts at: 3145 3145
Size: 4757
AhdI GACnn_n'nnGTC
Cuts at: 516 516
Size: 4757
AleI CACnn'nnGTG
Cuts at: 769 769
Size: 4757
AlwNI CAG_nnn'CTG
Cuts at: 1290 2062 3629 1290
Size: 772 1567 2418
Fragments arranged by size:
2418 1567 772
ApaI G_GGCC'C
Cuts at: 1457 1457
Size: 4757
ApaLI G'TGCA_C
Cuts at: 817 3527 817
Size: 2710 2047
ApoI r'AATT_y
Cuts at: 1761 4110 4366 1761
Size: 2349 256 2152
Fragments arranged by size:
2349 2152 256
AscI GG'CGCG_CC
Cuts at: 847 1574 847
Size: 727 4030
AseI AT'TA_AT
Cuts at: 20 20
Size: 4757
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4757
AvaI C'yCGr_G
Cuts at: 412 901 1054 1204 1348 1371 1449 1456 1777
Size: 489 153 150 144 23 78 7 321
Cuts at: 1777 412
Size: 3392
Fragments arranged by size:
3392 489 321 153 150 144 78 23
7
BamHI G'GATC_C
Cuts at: 437 1782 437
Size: 1345 3412
BanI G'GyrC_C
Cuts at: 925 1773 2187 2435 2470 925
Size: 848 414 248 35 3212
Fragments arranged by size:
3212 848 414 248 35
BanII G_rGCy'C
Cuts at: 193 413 1007 1145 1457 1717 1771 2801 193
Size: 220 594 138 312 260 54 1030 2149
Fragments arranged by size:
2149 1030 594 312 260 220 138 54
BbeI G_GCGC'C
Cuts at: 929 2191 2439 929
Size: 1262 248 3247
Fragments arranged by size:
3247 1262 248
BciVI GTATCCnnnnn_n'
Cuts at: 2655 3416 4757 2655
Size: 761 1341 2655
Fragments arranged by size:
2655 1341 761
BclI T'GATC_A
Cuts at: 2277 2277
Size: 4757
BglI GCCn_nnn'nGGC
Cuts at: 1044 1249 1565 1044
Size: 205 316 4236
Fragments arranged by size:
4236 316 205
BglII A'GATC_T
Cuts at: 2272 4309 2272
Size: 2037 2720
BlpI GC'TnA_GC
Cuts at: 1869 1869
Size: 4757
Bme1580I G_kGCm'C
Cuts at: 821 1274 1457 2382 2475 3531 821
Size: 453 183 925 93 1056 2047
Fragments arranged by size:
2047 1056 925 453 183 93
BmgBI CAC'GTC
Cuts at: 1314 1314
Size: 4757
BmrI ACTGGGnnnn_n'
Cuts at: 1969 2050 2385 4529 1969
Size: 81 335 2144 2197
Fragments arranged by size:
2197 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 595 663 3124 595
Size: 68 2461 2228
Fragments arranged by size:
2461 2228 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 664 1933 2812 3304 3602 664
Size: 1269 879 492 298 1819
Fragments arranged by size:
1819 1269 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1193 4461 1193
Size: 3268 1489
BsaAI yAC'GTr
Cuts at: 498 789 1749 2110 498
Size: 291 960 361 3145
Fragments arranged by size:
3145 960 361 291
BsaBI GATnn'nnATC
Cuts at: 6 2271 4308 6
Size: 2265 2037 455
Fragments arranged by size:
2265 2037 455
BsaHI Gr'CG_yC
Cuts at: 230 643 926 1036 1442 2188 2436 3138 4634
Size: 413 283 110 406 746 248 702 1496
Cuts at: 4634 230
Size: 353
Fragments arranged by size:
1496 746 702 413 406 353 283 248
110
BsaWI w'CCGG_w
Cuts at: 614 632 1946 2467 3145 3419 3566 3977 614
Size: 18 1314 521 678 274 147 411 1394
Fragments arranged by size:
1394 1314 678 521 411 274 147 18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 811 841 1369 1399 811
Size: 30 528 30 4169
Fragments arranged by size:
4169 528 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 178 178
Size: 4757
BseYI C'CCAG_C
Cuts at: 1510 1612 2182 2940 3517 4013 1510
Size: 102 570 758 577 496 2254
Fragments arranged by size:
2254 758 577 570 496 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 618 1051 618
Size: 433 4324
BsiEI CG_ry'CG
Cuts at: 104 345 1116 1244 2299 2345 3553 104
Size: 241 771 128 1055 46 1208 1308
Fragments arranged by size:
1308 1208 1055 771 241 128 46
BsiHKAI G_wGCw'C
Cuts at: 273 821 992 1007 1771 2549 2739 3531 273
Size: 548 171 15 764 778 190 792 1499
Fragments arranged by size:
1499 792 778 764 548 190 171 15
BsmI GAATG_Cn'
Cuts at: 723 723
Size: 4757
BsmBI CGTCTCn'nnnn_
Cuts at: 508 634 508
Size: 126 4631
Bsp1286I G_dGCh'C
Cuts at: 193 273 413 821 992 1007 1145 1274 1457
Size: 80 140 408 171 15 138 129 183
Cuts at: 1457 1717 1771 2382 2475 2549 2739 2801 3531
Size: 260 54 611 93 74 190 62 730
Cuts at: 3531 193
Size: 1419
Fragments arranged by size:
1419 730 611 408 260 190 183 171
140 138 129 93 80 74 62 54
15
BspEI T'CCGG_A
Cuts at: 3977 3977
Size: 4757
BspHI T'CATG_A
Cuts at: 3933 3933
Size: 4757
BspMI ACCTGCnnnn'nnnn_
Cuts at: 960 1807 2021 2323 2704 960
Size: 847 214 302 381 3013
Fragments arranged by size:
3013 847 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1356 1532 3049 3103 4587 4752 50
Size: 1306 176 1517 54 1484 165 55
Fragments arranged by size:
1517 1484 1306 176 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2669 2669
Size: 4757
BsrFI r'CCGG_y
Cuts at: 181 344 602 1588 2755 2936 3145 181
Size: 163 258 986 1167 181 209 1793
Fragments arranged by size:
1793 1167 986 258 209 181 163
BsrGI T'GTAC_A
Cuts at: 1328 1328
Size: 4757
BssHII G'CGCG_C
Cuts at: 847 1302 1471 1574 847
Size: 455 169 103 4030
Fragments arranged by size:
4030 455 169 103
BssSI C'ACGA_G
Cuts at: 266 991 3028 3386 266
Size: 725 2037 358 1637
Fragments arranged by size:
2037 1637 725 358
BstAPI GCAn_nnn'nTGC
Cuts at: 605 1106 2134 605
Size: 501 1028 3228
Fragments arranged by size:
3228 1028 501
BstBI TT'CG_AA
Cuts at: 110 3118 110
Size: 3008 1749
BstEII G'GTnAC_C
Cuts at: 1068 1068
Size: 4757
BstYI r'GATC_y
Cuts at: 1 437 1782 2249 2272 2607 2853 3865 3951
Size: 436 1345 467 23 335 246 1012 86
Cuts at: 3951 3963 4309 1
Size: 12 346 449
Fragments arranged by size:
1345 1012 467 449 436 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 301 301
Size: 4757
BtgI C'CryG_G
Cuts at: 125 239 359 917 1042 1497 1717 125
Size: 114 120 558 125 455 220 3165
Fragments arranged by size:
3165 558 455 220 125 120 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1413 2707 2888 3051 1413
Size: 1294 181 163 3119
Fragments arranged by size:
3119 1294 181 163
BtsI GCAGTG_nn'
Cuts at: 2000 2000
Size: 4757
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 918 953 918
Size: 35 4722
DraI TTT'AAA
Cuts at: 1756 1756
Size: 4757
DraIII CAC_nnn'GTG
Cuts at: 327 327
Size: 4757
DrdI GACnn_nn'nnGTC
Cuts at: 2463 3321 2463
Size: 858 3899
EaeI y'GGCC_r
Cuts at: 332 794 837 1113 1241 1316 1424 1586 2342
Size: 462 43 276 128 75 108 162 756
Cuts at: 2342 2516 2907 2934 4279 332
Size: 174 391 27 1345 810
Fragments arranged by size:
1345 810 756 462 391 276 174 162
128 108 75 43 27
EagI C'GGCC_G
Cuts at: 1113 1241 2342 1113
Size: 128 1101 3528
Fragments arranged by size:
3528 1101 128
EarI CTCTTCn'nnn_
Cuts at: 613 2780 2990 613
Size: 2167 210 2380
Fragments arranged by size:
2380 2167 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 433 1324 1377 2965 3275 3421 4567 433
Size: 891 53 1588 310 146 1146 623
Fragments arranged by size:
1588 1146 891 623 310 146 53
EcoICRI GAG'CTC
Cuts at: 1005 1769 1005
Size: 764 3993
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 733 1252 1318 1395 2582 3014 3761 4029
Size: 555 519 66 77 1187 432 747 268
Cuts at: 4029 178
Size: 906
Fragments arranged by size:
1187 906 747 555 519 432 268 77
66
EcoNI CCTnn'n_nnAGG
Cuts at: 160 160
Size: 4757
EcoO109I rG'GnC_Cy
Cuts at: 1453 1607 1896 1453
Size: 154 289 4314
Fragments arranged by size:
4314 289 154
EcoRI G'AATT_C
Cuts at: 1761 1761
Size: 4757
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2776 2776
Size: 4757
FseI GG_CCGG'CC
Cuts at: 1592 1592
Size: 4757
FspI TGC'GCA
Cuts at: 2538 2538
Size: 4757
HaeII r_GCGC'y
Cuts at: 929 1662 2191 2439 3461 4440 929
Size: 733 529 248 1022 979 1246
Fragments arranged by size:
1246 1022 979 733 529 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 502 534 2599 2629 2631 2661 502
Size: 32 2065 30 2 30 2598
Fragments arranged by size:
2598 2065 32 30 30 2
HincII GTy'rAC
Cuts at: 1796 1796
Size: 4757
KasI G'GCGC_C
Cuts at: 925 2187 2435 925
Size: 1262 248 3247
Fragments arranged by size:
3247 1262 248
KpnI G_GTAC'C
Cuts at: 1777 1777
Size: 4757
MluI A'CGCG_T
Cuts at: 4190 4190
Size: 4757
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 320 335 827 3428 3612 320
Size: 15 492 2601 184 1465
Fragments arranged by size:
2601 1465 492 184 15
MscI TGG'CCA
Cuts at: 334 1426 2518 334
Size: 1092 1092 2573
Fragments arranged by size:
2573 1092 1092
MslI CAynn'nnrTG
Cuts at: 769 814 1027 2746 2873 769
Size: 45 213 1719 127 2653
Fragments arranged by size:
2653 1719 213 127 45
MspA1I CmG'CkG
Cuts at: 146 171 241 1044 1422 1478 1499 1868 2182
Size: 25 70 803 378 56 21 369 314
Cuts at: 2182 2542 3555 3800 146
Size: 360 1013 245 1103
Fragments arranged by size:
1103 1013 803 378 369 360 314 245
70 56 25 21
NaeI GCC'GGC
Cuts at: 183 1590 2938 183
Size: 1407 1348 2002
Fragments arranged by size:
2002 1407 1348
NarI GG'CG_CC
Cuts at: 926 2188 2436 926
Size: 1262 248 3247
Fragments arranged by size:
3247 1262 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 4757
NgoMIV G'CCGG_C
Cuts at: 181 1588 2936 181
Size: 1407 1348 2002
Fragments arranged by size:
2002 1407 1348
NspI r_CATG'y
Cuts at: 741 813 1810 2310 3217 741
Size: 72 997 500 907 2281
Fragments arranged by size:
2281 997 907 500 72
PciI A'CATG_T
Cuts at: 809 3213 809
Size: 2404 2353
PflMI CCAn_nnn'nTGG
Cuts at: 756 756
Size: 4757
PfoI T'CCnGG_A
Cuts at: 202 224 202
Size: 22 4735
PmeI GTTT'AAAC
Cuts at: 1756 1756
Size: 4757
PmlI CAC'GTG
Cuts at: 789 789
Size: 4757
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3896 3928 3896
Size: 32 4725
PpuMI rG'GwC_Cy
Cuts at: 1607 1607
Size: 4757
PspOMI G'GGCC_C
Cuts at: 1453 1453
Size: 4757
PstI C_TGCA'G
Cuts at: 1483 1804 2489 1483
Size: 321 685 3751
Fragments arranged by size:
3751 685 321
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4757
PvuII CAG'CTG
Cuts at: 1478 2182 2542 1478
Size: 704 360 3693
Fragments arranged by size:
3693 704 360
RsrII CG'GwC_CG
Cuts at: 2952 2952
Size: 4757
SacI G_AGCT'C
Cuts at: 1007 1771 1007
Size: 764 3993
SacII CC_GC'GG
Cuts at: 242 1045 1500 242
Size: 803 455 3499
Fragments arranged by size:
3499 803 455
SalI G'TCGA_C
Cuts at: 1794 1794
Size: 4757
SanDI GG'GwC_CC
Cuts at: 1607 1607
Size: 4757
SapI GCTCTTCn'nnn_
Cuts at: 613 2780 2990 613
Size: 2167 210 2380
Fragments arranged by size:
2380 2167 210
SbfI CC_TGCA'GG
Cuts at: 1804 1804
Size: 4757
ScaI AGT'ACT
Cuts at: 4274 4274
Size: 4757
SfcI C'TryA_G
Cuts at: 33 1171 1479 1800 2485 3478 3669 33
Size: 1138 308 321 685 993 191 1121
Fragments arranged by size:
1138 1121 993 685 321 308 191
SfoI GGC'GCC
Cuts at: 927 2189 2437 927
Size: 1262 248 3247
Fragments arranged by size:
3247 1262 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4757
SmaI CCC'GGG
Cuts at: 414 903 1056 1350 1451 1779 414
Size: 489 153 294 101 328 3392
Fragments arranged by size:
3392 489 328 294 153 101
SmlI C'TyrA_G
Cuts at: 679 1912 2827 3319 3581 679
Size: 1233 915 492 262 1855
Fragments arranged by size:
1855 1233 915 492 262
SnaBI TAC'GTA
Cuts at: 1749 1749
Size: 4757
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4757
SphI G_CATG'C
Cuts at: 1810 2310 1810
Size: 500 4257
StuI AGG'CCT
Cuts at: 1703 1703
Size: 4757
StyI C'CwwG_G
Cuts at: 125 1217 1652 1891 2245 125
Size: 1092 435 239 354 2637
Fragments arranged by size:
2637 1092 435 354 239
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 419 598 1394 2340 2473 2932 3141 419
Size: 179 796 946 133 459 209 2035
Fragments arranged by size:
2035 946 796 459 209 179 133
TatI w'GTAC_w
Cuts at: 1328 4272 1328
Size: 2944 1813
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 609 641 811 843 1369 1401 4274 4306 609
Size: 32 170 32 526 32 2873 32 1060
Fragments arranged by size:
2873 1060 526 170 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 655 2554 655
Size: 1899 2858
XbaI T'CTAG_A
Cuts at: 1788 1788
Size: 4757
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2252 2252
Size: 4757
XmaI C'CCGG_G
Cuts at: 412 901 1054 1348 1449 1777 412
Size: 489 153 294 101 328 3392
Fragments arranged by size:
3392 489 328 294 153 101
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI AvrII BaeI
BbsI BbvCI BcgI BfrBI BmtI BpmI Bpu10I BsiWI
BstXI BstZ17I ClaI EcoRV FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PshAI
PsiI PspXI PsrI SexAI SfiI SgrAI SrfI SspI
SwaI XhoI XmnI ZraI