Restriction map for
pF1KSDA1443
(Circular) (Six-base) MAPSORT of: pF1KSDA1443.seq Check: 3610 from: 1 to: 4784
pF1KSDA1443 4784 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 341 1596 341
Size: 1255 3529
AccI GT'mk_AC
Cuts at: 506 1822 506
Size: 1316 3468
Acc65I G'GTAC_C
Cuts at: 1800 1800
Size: 4784
AclI AA'CG_TT
Cuts at: 4624 4624
Size: 4784
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 194 330 494 2609 3041 3788 4056 194
Size: 136 164 2115 432 747 268 922
Fragments arranged by size:
2115 922 747 432 268 164 136
AflIII A'CryG_T
Cuts at: 2136 3240 4217 2136
Size: 1104 977 2703
Fragments arranged by size:
2703 1104 977
AgeI A'CCGG_T
Cuts at: 3172 3172
Size: 4784
AlwNI CAG_nnn'CTG
Cuts at: 1008 1399 1611 2089 3656 1008
Size: 391 212 478 1567 2136
Fragments arranged by size:
2136 1567 478 391 212
ApaLI G'TGCA_C
Cuts at: 3554 3554
Size: 4784
ApoI r'AATT_y
Cuts at: 1788 4137 4393 1788
Size: 2349 256 2179
Fragments arranged by size:
2349 2179 256
AseI AT'TA_AT
Cuts at: 20 928 20
Size: 908 3876
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4784
AvaI C'yCGr_G
Cuts at: 151 491 497 902 1804 151
Size: 340 6 405 902 3131
Fragments arranged by size:
3131 902 405 340 6
BamHI G'GATC_C
Cuts at: 1809 1809
Size: 4784
BanI G'GyrC_C
Cuts at: 193 579 1402 1800 2214 2462 2497 193
Size: 386 823 398 414 248 35 2480
Fragments arranged by size:
2480 823 414 398 386 248 35
BanII G_rGCy'C
Cuts at: 248 498 689 1798 2828 248
Size: 250 191 1109 1030 2204
Fragments arranged by size:
2204 1109 1030 250 191
BbeI G_GCGC'C
Cuts at: 2218 2466 2218
Size: 248 4536
BbvCI CC'TCA_GC
Cuts at: 1637 1646 1637
Size: 9 4775
BciVI GTATCCnnnnn_n'
Cuts at: 2682 3443 4784 2682
Size: 761 1341 2682
Fragments arranged by size:
2682 1341 761
BclI T'GATC_A
Cuts at: 1760 2304 1760
Size: 544 4240
BglI GCCn_nnn'nGGC
Cuts at: 153 1125 153
Size: 972 3812
BglII A'GATC_T
Cuts at: 2299 4336 2299
Size: 2037 2747
BlpI GC'TnA_GC
Cuts at: 1594 1896 1594
Size: 302 4482
Bme1580I G_kGCm'C
Cuts at: 182 1269 1407 2409 2502 3558 182
Size: 1087 138 1002 93 1056 1408
Fragments arranged by size:
1408 1087 1056 1002 138 93
BmrI ACTGGGnnnn_n'
Cuts at: 1073 1542 1996 2077 2412 4556 1073
Size: 469 454 81 335 2144 1301
Fragments arranged by size:
2144 1301 469 454 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 705 3151 705
Size: 2446 2338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 575 587 1027 575
Size: 12 440 4332
Fragments arranged by size:
4332 440 12
Bpu10I CC'TnA_GC
Cuts at: 1637 1646 1637
Size: 9 4775
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1960 2839 3331 3629 1960
Size: 879 492 298 3115
Fragments arranged by size:
3115 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 229 4488 229
Size: 4259 525
BsaAI yAC'GTr
Cuts at: 1776 2137 1776
Size: 361 4423
BsaBI GATnn'nnATC
Cuts at: 6 2298 4335 6
Size: 2292 2037 455
Fragments arranged by size:
2292 2037 455
BsaHI Gr'CG_yC
Cuts at: 2215 2463 3165 4661 2215
Size: 248 702 1496 2338
Fragments arranged by size:
2338 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1506 1973 2494 3172 3446 3593 4004 1506
Size: 467 521 678 274 147 411 2286
Fragments arranged by size:
2286 678 521 467 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 186 216 186
Size: 30 4754
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 286 575 578 589 697 964 1696 1699 1732
Size: 289 3 11 108 267 732 3 33
Cuts at: 1732 1735 1738 1741 286
Size: 3 3 3 3329
Fragments arranged by size:
3329 732 289 267 108 33 11 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 137 364 440 890 1422 1601 2209 2967 3544
Size: 227 76 450 532 179 608 758 577
Cuts at: 3544 4040 137
Size: 496 881
Fragments arranged by size:
881 758 608 577 532 496 450 227
179 76
BsiEI CG_ry'CG
Cuts at: 104 2326 2372 3580 104
Size: 2222 46 1208 1308
Fragments arranged by size:
2222 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1798 2576 2766 3558 1798
Size: 778 190 792 3024
Fragments arranged by size:
3024 792 778 190
BsmI GAATG_Cn'
Cuts at: 760 760
Size: 4784
Bsp1286I G_dGCh'C
Cuts at: 182 248 498 689 1269 1407 1798 2409 2502
Size: 66 250 191 580 138 391 611 93
Cuts at: 2502 2576 2766 2828 3558 182
Size: 74 190 62 730 1408
Fragments arranged by size:
1408 730 611 580 391 250 191 190
138 93 74 66 62
BspEI T'CCGG_A
Cuts at: 4004 4004
Size: 4784
BspHI T'CATG_A
Cuts at: 3960 3960
Size: 4784
BspMI ACCTGCnnnn'nnnn_
Cuts at: 341 1596 1834 2048 2350 2731 341
Size: 1255 238 214 302 381 2394
Fragments arranged by size:
2394 1255 381 302 238 214
BsrBI CCG'CTC
Cuts at: 50 1454 3076 3130 4614 4779 50
Size: 1404 1622 54 1484 165 55
Fragments arranged by size:
1622 1484 1404 165 55 54
BsrDI GCAATG_nn'
Cuts at: 330 377 2696 330
Size: 47 2319 2418
Fragments arranged by size:
2418 2319 47
BsrFI r'CCGG_y
Cuts at: 2782 2963 3172 2782
Size: 181 209 4394
Fragments arranged by size:
4394 209 181
BssSI C'ACGA_G
Cuts at: 3055 3413 3055
Size: 358 4426
BstAPI GCAn_nnn'nTGC
Cuts at: 379 931 2161 379
Size: 552 1230 3002
Fragments arranged by size:
3002 1230 552
BstBI TT'CG_AA
Cuts at: 110 3145 110
Size: 3035 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 847 847
Size: 4784
BstYI r'GATC_y
Cuts at: 1 1809 2276 2299 2634 2880 3892 3978 3990
Size: 1808 467 23 335 246 1012 86 12
Cuts at: 3990 4336 1
Size: 346 449
Fragments arranged by size:
1808 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 680 680
Size: 4784
BtgI C'CryG_G
Cuts at: 125 125
Size: 4784
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2734 2915 3078 2734
Size: 181 163 4440
Fragments arranged by size:
4440 181 163
BtsI GCAGTG_nn'
Cuts at: 703 759 807 2027 703
Size: 56 48 1220 3460
Fragments arranged by size:
3460 1220 56 48
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1357 1392 1357
Size: 35 4749
DraI TTT'AAA
Cuts at: 1783 1783
Size: 4784
DraIII CAC_nnn'GTG
Cuts at: 1081 1273 1081
Size: 192 4592
DrdI GACnn_nn'nnGTC
Cuts at: 2490 3348 2490
Size: 858 3926
EaeI y'GGCC_r
Cuts at: 2369 2543 2934 2961 4306 2369
Size: 174 391 27 1345 2847
Fragments arranged by size:
2847 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2369 2369
Size: 4784
EarI CTCTTCn'nnn_
Cuts at: 479 981 996 1667 1673 1709 2807 3017 479
Size: 502 15 671 6 36 1098 210 2246
Fragments arranged by size:
2246 1098 671 502 210 36 15 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2992 3302 3448 4594 2992
Size: 310 146 1146 3182
Fragments arranged by size:
3182 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1796 1796
Size: 4784
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 194 330 494 575 587 1027 2609 3041 3788
Size: 136 164 81 12 440 1582 432 747
Cuts at: 3788 4056 194
Size: 268 922
Fragments arranged by size:
1582 922 747 440 432 268 164 136
81 12
EcoNI CCTnn'n_nnAGG
Cuts at: 811 1524 811
Size: 713 4071
EcoO109I rG'GnC_Cy
Cuts at: 628 832 1923 628
Size: 204 1091 3489
Fragments arranged by size:
3489 1091 204
EcoRI G'AATT_C
Cuts at: 1788 1788
Size: 4784
EcoRV GAT'ATC
Cuts at: 1566 1566
Size: 4784
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2803 2803
Size: 4784
FspI TGC'GCA
Cuts at: 2565 2565
Size: 4784
HaeII r_GCGC'y
Cuts at: 447 2218 2466 3488 4467 447
Size: 1771 248 1022 979 764
Fragments arranged by size:
1771 1022 979 764 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 943 975 1467 1499 2626 2656 2658 2688 943
Size: 32 492 32 1127 30 2 30 3039
Fragments arranged by size:
3039 1127 492 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1823 1823
Size: 4784
KasI G'GCGC_C
Cuts at: 2214 2462 2214
Size: 248 4536
KpnI G_GTAC'C
Cuts at: 1804 1804
Size: 4784
MfeI C'AATT_G
Cuts at: 920 1573 920
Size: 653 4131
MluI A'CGCG_T
Cuts at: 4217 4217
Size: 4784
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 658 3455 3639 658
Size: 2797 184 1803
Fragments arranged by size:
2797 1803 184
MscI TGG'CCA
Cuts at: 2545 2545
Size: 4784
MslI CAynn'nnrTG
Cuts at: 2773 2900 2773
Size: 127 4657
MspA1I CmG'CkG
Cuts at: 221 241 452 1227 1645 1895 2209 2569 3582
Size: 20 211 775 418 250 314 360 1013
Cuts at: 3582 3827 221
Size: 245 1178
Fragments arranged by size:
1178 1013 775 418 360 314 250 245
211 20
NaeI GCC'GGC
Cuts at: 2965 2965
Size: 4784
NarI GG'CG_CC
Cuts at: 2215 2463 2215
Size: 248 4536
NcoI C'CATG_G
Cuts at: 125 125
Size: 4784
NgoMIV G'CCGG_C
Cuts at: 2963 2963
Size: 4784
NspI r_CATG'y
Cuts at: 1837 2337 3244 1837
Size: 500 907 3377
Fragments arranged by size:
3377 907 500
PciI A'CATG_T
Cuts at: 3240 3240
Size: 4784
PflMI CCAn_nnn'nTGG
Cuts at: 1008 1008
Size: 4784
PfoI T'CCnGG_A
Cuts at: 865 865
Size: 4784
PmeI GTTT'AAAC
Cuts at: 1783 1783
Size: 4784
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 320 352 3923 3955 320
Size: 32 3571 32 1149
Fragments arranged by size:
3571 1149 32 32
PpuMI rG'GwC_Cy
Cuts at: 628 832 628
Size: 204 4580
PspXI vC'TCGA_Gb
Cuts at: 491 491
Size: 4784
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1076 1108 1076
Size: 32 4752
PstI C_TGCA'G
Cuts at: 1831 2516 1831
Size: 685 4099
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4784
PvuII CAG'CTG
Cuts at: 1227 1645 2209 2569 1227
Size: 418 564 360 3442
Fragments arranged by size:
3442 564 418 360
RsrII CG'GwC_CG
Cuts at: 2979 2979
Size: 4784
SacI G_AGCT'C
Cuts at: 1798 1798
Size: 4784
SalI G'TCGA_C
Cuts at: 1821 1821
Size: 4784
SanDI GG'GwC_CC
Cuts at: 832 832
Size: 4784
SapI GCTCTTCn'nnn_
Cuts at: 2807 3017 2807
Size: 210 4574
SbfI CC_TGCA'GG
Cuts at: 1831 1831
Size: 4784
ScaI AGT'ACT
Cuts at: 4301 4301
Size: 4784
SexAI A'CCwGG_T
Cuts at: 1146 1398 1146
Size: 252 4532
SfcI C'TryA_G
Cuts at: 33 277 1827 2512 3505 3696 33
Size: 244 1550 685 993 191 1121
Fragments arranged by size:
1550 1121 993 685 244 191
SfoI GGC'GCC
Cuts at: 2216 2464 2216
Size: 248 4536
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4784
SmaI CCC'GGG
Cuts at: 153 1806 153
Size: 1653 3131
SmlI C'TyrA_G
Cuts at: 491 1939 2854 3346 3608 491
Size: 1448 915 492 262 1667
Fragments arranged by size:
1667 1448 915 492 262
SnaBI TAC'GTA
Cuts at: 1776 1776
Size: 4784
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4784
SphI G_CATG'C
Cuts at: 1837 2337 1837
Size: 500 4284
SrfI GCCC'GGGC
Cuts at: 153 153
Size: 4784
StyI C'CwwG_G
Cuts at: 125 1122 1520 1918 2272 125
Size: 997 398 398 354 2637
Fragments arranged by size:
2637 997 398 398 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1092 1615 2367 2500 2959 3168 1092
Size: 523 752 133 459 209 2708
Fragments arranged by size:
2708 752 523 459 209 133
TatI w'GTAC_w
Cuts at: 4299 4299
Size: 4784
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 186 218 4301 4333 186
Size: 32 4083 32 637
Fragments arranged by size:
4083 637 32 32
Tth111I GACn'n_nGTC
Cuts at: 2581 2581
Size: 4784
XbaI T'CTAG_A
Cuts at: 1662 1815 1662
Size: 153 4631
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1224 2279 1224
Size: 1055 3729
XhoI C'TCGA_G
Cuts at: 491 491
Size: 4784
XmaI C'CCGG_G
Cuts at: 151 1804 151
Size: 1653 3131
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SrfI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI
BsgI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I ClaI
FseI FspAI HindIII HpaI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PshAI PsiI PspOMI SacII
SfiI SgrAI SspI StuI SwaI XmnI ZraI