Restriction map for
pF1KSDA1453
(Circular) (Six-base) MAPSORT of: pF1KSDA1453.seq Check: 2390 from: 1 to: 6500
pF1KSDA1453 6500 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 523 2361 2814 523
Size: 1838 453 4209
Fragments arranged by size:
4209 1838 453
AatII G_ACGT'C
Cuts at: 938 2440 938
Size: 1502 4998
AccI GT'mk_AC
Cuts at: 3538 3538
Size: 6500
Acc65I G'GTAC_C
Cuts at: 1307 3516 1307
Size: 2209 4291
AclI AA'CG_TT
Cuts at: 6340 6340
Size: 6500
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1110 1592 1639 1933 2140 2373 3406 4325 4757
Size: 482 47 294 207 233 1033 919 432
Cuts at: 4757 5504 5772 1110
Size: 747 268 1838
Fragments arranged by size:
1838 1033 919 747 482 432 294 268
233 207 47
AfeI AGC'GCT
Cuts at: 1066 1932 1066
Size: 866 5634
AflIII A'CryG_T
Cuts at: 389 879 1025 3852 4956 5933 389
Size: 490 146 2827 1104 977 956
Fragments arranged by size:
2827 1104 977 956 490 146
AgeI A'CCGG_T
Cuts at: 4888 4888
Size: 6500
AhdI GACnn_n'nnGTC
Cuts at: 2499 2499
Size: 6500
AleI CACnn'nnGTG
Cuts at: 347 347
Size: 6500
AlwNI CAG_nnn'CTG
Cuts at: 1782 1894 2592 3098 3204 3805 5372 1782
Size: 112 698 506 106 601 1567 2910
Fragments arranged by size:
2910 1567 698 601 506 112 106
ApaLI G'TGCA_C
Cuts at: 1240 5270 1240
Size: 4030 2470
ApoI r'AATT_y
Cuts at: 854 1165 1391 3376 3504 5853 6109 854
Size: 311 226 1985 128 2349 256 1245
Fragments arranged by size:
2349 1985 1245 311 256 226 128
AseI AT'TA_AT
Cuts at: 20 20
Size: 6500
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6500
AvaI C'yCGr_G
Cuts at: 1245 1414 1461 1656 3085 3520 1245
Size: 169 47 195 1429 435 4225
Fragments arranged by size:
4225 1429 435 195 169 47
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1291 1324 1291
Size: 33 6467
BamHI G'GATC_C
Cuts at: 3525 3525
Size: 6500
BanI G'GyrC_C
Cuts at: 1307 3516 3930 4178 4213 1307
Size: 2209 414 248 35 3594
Fragments arranged by size:
3594 2209 414 248 35
BanII G_rGCy'C
Cuts at: 1837 1954 2537 2548 2599 2740 3514 4544 1837
Size: 117 583 11 51 141 774 1030 3793
Fragments arranged by size:
3793 1030 774 583 141 117 51 11
BbeI G_GCGC'C
Cuts at: 3934 4182 3934
Size: 248 6252
BbsI GAAGACnn'nnnn_
Cuts at: 468 3299 468
Size: 2831 3669
BbvCI CC'TCA_GC
Cuts at: 3475 3475
Size: 6500
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 951 985 2457 2491 951
Size: 34 1472 34 4960
Fragments arranged by size:
4960 1472 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4398 5159 6500 4398
Size: 761 1341 4398
Fragments arranged by size:
4398 1341 761
BclI T'GATC_A
Cuts at: 4020 4020
Size: 6500
BglI GCCn_nnn'nGGC
Cuts at: 803 2209 2589 2638 803
Size: 1406 380 49 4665
Fragments arranged by size:
4665 1406 380 49
BglII A'GATC_T
Cuts at: 1813 4015 6052 1813
Size: 2202 2037 2261
Fragments arranged by size:
2261 2202 2037
BlpI GC'TnA_GC
Cuts at: 3145 3612 3145
Size: 467 6033
Bme1580I G_kGCm'C
Cuts at: 1244 1271 1589 4125 4218 5274 1244
Size: 27 318 2536 93 1056 2470
Fragments arranged by size:
2536 2470 1056 318 93 27
BmgBI CAC'GTC
Cuts at: 2606 2606
Size: 6500
BmrI ACTGGGnnnn_n'
Cuts at: 205 3342 3712 3793 4128 6272 205
Size: 3137 370 81 335 2144 433
Fragments arranged by size:
3137 2144 433 370 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 886 4867 886
Size: 3981 2519
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 964 1413 1938 1947 304
Size: 660 449 525 9 4857
Fragments arranged by size:
4857 660 525 449 9
Bpu10I CC'TnA_GC
Cuts at: 1775 1874 2134 3475 1775
Size: 99 260 1341 4800
Fragments arranged by size:
4800 1341 260 99
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1093 3676 4555 5047 5345 1093
Size: 2583 879 492 298 2248
Fragments arranged by size:
2583 2248 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 695 3310 6204 695
Size: 2615 2894 991
Fragments arranged by size:
2894 2615 991
BsaAI yAC'GTr
Cuts at: 392 1284 1374 3492 3853 392
Size: 892 90 2118 361 3039
Fragments arranged by size:
3039 2118 892 361 90
BsaBI GATnn'nnATC
Cuts at: 6 2957 3038 4014 6051 6
Size: 2951 81 976 2037 455
Fragments arranged by size:
2951 2037 976 455 81
BsaHI Gr'CG_yC
Cuts at: 779 935 2437 3931 4179 4881 6377 779
Size: 156 1502 1494 248 702 1496 902
Fragments arranged by size:
1502 1496 1494 902 702 248 156
BsaWI w'CCGG_w
Cuts at: 1161 3689 4210 4888 5162 5309 5720 1161
Size: 2528 521 678 274 147 411 1941
Fragments arranged by size:
2528 1941 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 439 469 562 592 1862 1892 2154 2184 2625
Size: 30 93 30 1270 30 262 30 441
Cuts at: 2625 2655 439
Size: 30 4284
Fragments arranged by size:
4284 1270 441 262 93 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 204 1431 1434 1736 2162 2448 2886 3408 204
Size: 1227 3 302 426 286 438 522 3296
Fragments arranged by size:
3296 1227 522 438 426 302 286 3
BseYI C'CCAG_C
Cuts at: 417 2592 3456 3925 4683 5260 5756 417
Size: 2175 864 469 758 577 496 1161
Fragments arranged by size:
2175 1161 864 758 577 496 469
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2896 2896
Size: 6500
BsiEI CG_ry'CG
Cuts at: 104 168 1458 2234 4042 4088 5296 104
Size: 64 1290 776 1808 46 1208 1308
Fragments arranged by size:
1808 1308 1290 1208 776 64 46
BsiHKAI G_wGCw'C
Cuts at: 389 892 978 1244 1976 3514 4292 4482 5274
Size: 503 86 266 732 1538 778 190 792
Cuts at: 5274 389
Size: 1615
Fragments arranged by size:
1615 1538 792 778 732 503 266 190
86
BsmI GAATG_Cn'
Cuts at: 1023 1637 1023
Size: 614 5886
BsmBI CGTCTCn'nnnn_
Cuts at: 693 912 2596 693
Size: 219 1684 4597
Fragments arranged by size:
4597 1684 219
Bsp1286I G_dGCh'C
Cuts at: 389 892 978 1244 1271 1589 1837 1954 1976
Size: 503 86 266 27 318 248 117 22
Cuts at: 1976 2537 2548 2599 2740 3514 4125 4218 4292
Size: 561 11 51 141 774 611 93 74
Cuts at: 4292 4482 4544 5274 389
Size: 190 62 730 1615
Fragments arranged by size:
1615 774 730 611 561 503 318 266
248 190 141 117 93 86 74 62
51 27 22 11
BspEI T'CCGG_A
Cuts at: 1161 5720 1161
Size: 4559 1941
BspHI T'CATG_A
Cuts at: 5676 5676
Size: 6500
BspMI ACCTGCnnnn'nnnn_
Cuts at: 523 581 2361 2814 3550 3764 4066 4447 523
Size: 58 1780 453 736 214 302 381 2576
Fragments arranged by size:
2576 1780 736 453 381 302 214 58
BsrBI CCG'CTC
Cuts at: 50 471 2026 3180 4792 4846 6330 6495 50
Size: 421 1555 1154 1612 54 1484 165 55
Fragments arranged by size:
1612 1555 1484 1154 421 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1302 1506 2244 2404 4412 1302
Size: 204 738 160 2008 3390
Fragments arranged by size:
3390 2008 738 204 160
BsrFI r'CCGG_y
Cuts at: 363 2233 4498 4679 4888 363
Size: 1870 2265 181 209 1975
Fragments arranged by size:
2265 1975 1870 209 181
BsrGI T'GTAC_A
Cuts at: 2744 2744
Size: 6500
BssSI C'ACGA_G
Cuts at: 4771 5129 4771
Size: 358 6142
BstAPI GCAn_nnn'nTGC
Cuts at: 432 515 3466 3877 432
Size: 83 2951 411 3055
Fragments arranged by size:
3055 2951 411 83
BstBI TT'CG_AA
Cuts at: 110 4861 110
Size: 4751 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 349 505 1201 2190 349
Size: 156 696 989 4659
Fragments arranged by size:
4659 989 696 156
BstYI r'GATC_y
Cuts at: 1 947 1813 3525 3992 4015 4350 4596 5608
Size: 946 866 1712 467 23 335 246 1012
Cuts at: 5608 5694 5706 6052 1
Size: 86 12 346 449
Fragments arranged by size:
1712 1012 946 866 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2480 2480
Size: 6500
BtgI C'CryG_G
Cuts at: 443 2446 2646 2945 2969 3320 443
Size: 2003 200 299 24 351 3623
Fragments arranged by size:
3623 2003 351 299 200 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4450 4631 4794 4450
Size: 181 163 6156
Fragments arranged by size:
6156 181 163
BtsI GCAGTG_nn'
Cuts at: 387 1892 2633 3743 387
Size: 1505 741 1110 3144
Fragments arranged by size:
3144 1505 1110 741
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2498 2533 2498
Size: 35 6465
DraI TTT'AAA
Cuts at: 3499 3499
Size: 6500
DraIII CAC_nnn'GTG
Cuts at: 2222 2222
Size: 6500
DrdI GACnn_nn'nnGTC
Cuts at: 4206 5064 4206
Size: 858 5642
EaeI y'GGCC_r
Cuts at: 165 365 1455 1979 2070 2817 2844 4085 4259
Size: 200 1090 524 91 747 27 1241 174
Cuts at: 4259 4650 4677 6022 165
Size: 391 27 1345 643
Fragments arranged by size:
1345 1241 1090 747 643 524 391 200
174 91 27 27
EagI C'GGCC_G
Cuts at: 165 1455 4085 165
Size: 1290 2630 2580
Fragments arranged by size:
2630 2580 1290
EarI CTCTTCn'nnn_
Cuts at: 1568 2551 2647 2653 2969 3023 3335 3385 4523
Size: 983 96 6 316 54 312 50 1138
Cuts at: 4523 4733 1568
Size: 210 3335
Fragments arranged by size:
3335 1138 983 316 312 210 96 54
50 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4708 5018 5164 6310 4708
Size: 310 146 1146 4898
Fragments arranged by size:
4898 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3512 3512
Size: 6500
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 964 1110 1413 1592 1639 1933 1938 1947
Size: 660 146 303 179 47 294 5 9
Cuts at: 1947 2140 2373 3406 4325 4757 5504 5772 304
Size: 193 233 1033 919 432 747 268 1032
Fragments arranged by size:
1033 1032 919 747 660 432 303 294
268 233 193 179 146 47 9 5
EcoNI CCTnn'n_nnAGG
Cuts at: 2707 2914 2707
Size: 207 6293
EcoO109I rG'GnC_Cy
Cuts at: 153 228 1419 1661 2001 2493 2919 3153 3639
Size: 75 1191 242 340 492 426 234 486
Cuts at: 3639 153
Size: 3014
Fragments arranged by size:
3014 1191 492 486 426 340 242 234
75
EcoRI G'AATT_C
Cuts at: 1165 1391 3504 1165
Size: 226 2113 4161
Fragments arranged by size:
4161 2113 226
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4519 4519
Size: 6500
FspI TGC'GCA
Cuts at: 3102 4281 3102
Size: 1179 5321
HaeII r_GCGC'y
Cuts at: 1068 1934 2476 2679 2702 3934 4182 5204 6183
Size: 866 542 203 23 1232 248 1022 979
Cuts at: 6183 1068
Size: 1385
Fragments arranged by size:
1385 1232 1022 979 866 542 248 203
23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 439 471 1461 1493 2485 2517 3169 3201 4342
Size: 32 990 32 992 32 652 32 1141
Cuts at: 4342 4372 4374 4404 439
Size: 30 2 30 2535
Fragments arranged by size:
2535 1141 992 990 652 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2487 3539 2487
Size: 1052 5448
HindIII A'AGCT_T
Cuts at: 202 612 3227 202
Size: 410 2615 3475
Fragments arranged by size:
3475 2615 410
KasI G'GCGC_C
Cuts at: 3930 4178 3930
Size: 248 6252
KpnI G_GTAC'C
Cuts at: 1311 3520 1311
Size: 2209 4291
MluI A'CGCG_T
Cuts at: 879 5933 879
Size: 5054 1446
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 797 1112 2297 5171 5355 797
Size: 315 1185 2874 184 1942
Fragments arranged by size:
2874 1942 1185 315 184
MscI TGG'CCA
Cuts at: 2072 2819 4261 2072
Size: 747 1442 4311
Fragments arranged by size:
4311 1442 747
MslI CAynn'nnrTG
Cuts at: 347 392 1696 4489 4616 347
Size: 45 1304 2793 127 2231
Fragments arranged by size:
2793 2231 1304 127 45
MspA1I CmG'CkG
Cuts at: 282 599 668 1130 1256 2068 2692 2769 2776
Size: 317 69 462 126 812 624 77 7
Cuts at: 2776 2936 3018 3098 3611 3925 4285 5298 5543
Size: 160 82 80 513 314 360 1013 245
Cuts at: 5543 282
Size: 1239
Fragments arranged by size:
1239 1013 812 624 513 462 360 317
314 245 160 126 82 80 77 69
7
NaeI GCC'GGC
Cuts at: 4681 4681
Size: 6500
NarI GG'CG_CC
Cuts at: 3931 4179 3931
Size: 248 6252
NcoI C'CATG_G
Cuts at: 2945 2969 2945
Size: 24 6476
NgoMIV G'CCGG_C
Cuts at: 4679 4679
Size: 6500
NspI r_CATG'y
Cuts at: 592 624 1029 1701 3553 4053 4960 592
Size: 32 405 672 1852 500 907 2132
Fragments arranged by size:
2132 1852 907 672 500 405 32
PasI CC'CwG_GG
Cuts at: 1611 2431 1611
Size: 820 5680
PciI A'CATG_T
Cuts at: 1025 4956 1025
Size: 3931 2569
PflMI CCAn_nnn'nTGG
Cuts at: 1525 2826 1525
Size: 1301 5199
PfoI T'CCnGG_A
Cuts at: 1429 1594 3197 1429
Size: 165 1603 4732
Fragments arranged by size:
4732 1603 165
PmeI GTTT'AAAC
Cuts at: 3499 3499
Size: 6500
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5639 5671 5639
Size: 32 6468
PpuMI rG'GwC_Cy
Cuts at: 228 1661 2493 2919 228
Size: 1433 832 426 3809
Fragments arranged by size:
3809 1433 832 426
PshAI GACnn'nnGTC
Cuts at: 2246 2246
Size: 6500
PstI C_TGCA'G
Cuts at: 2708 3547 4232 2708
Size: 839 685 4976
Fragments arranged by size:
4976 839 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6500
PvuII CAG'CTG
Cuts at: 282 599 1256 2692 2769 2936 3098 3925 4285
Size: 317 657 1436 77 167 162 827 360
Cuts at: 4285 282
Size: 2497
Fragments arranged by size:
2497 1436 827 657 360 317 167 162
77
RsrII CG'GwC_CG
Cuts at: 4695 4695
Size: 6500
SacI G_AGCT'C
Cuts at: 3514 3514
Size: 6500
SalI G'TCGA_C
Cuts at: 3537 3537
Size: 6500
SanDI GG'GwC_CC
Cuts at: 1661 1661
Size: 6500
SapI GCTCTTCn'nnn_
Cuts at: 4523 4733 4523
Size: 210 6290
SbfI CC_TGCA'GG
Cuts at: 2708 3547 2708
Size: 839 5661
ScaI AGT'ACT
Cuts at: 6017 6017
Size: 6500
SfcI C'TryA_G
Cuts at: 33 1251 1886 2704 3543 4228 5221 5412 33
Size: 1218 635 818 839 685 993 191 1121
Fragments arranged by size:
1218 1121 993 839 818 685 635 191
SfoI GGC'GCC
Cuts at: 3932 4180 3932
Size: 248 6252
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6500
SmaI CCC'GGG
Cuts at: 3522 3522
Size: 6500
SmlI C'TyrA_G
Cuts at: 1108 3655 4570 5062 5324 1108
Size: 2547 915 492 262 2284
Fragments arranged by size:
2547 2284 915 492 262
SnaBI TAC'GTA
Cuts at: 3492 3492
Size: 6500
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6500
SphI G_CATG'C
Cuts at: 592 624 3553 4053 592
Size: 32 2929 500 3039
Fragments arranged by size:
3039 2929 500 32
SspI AAT'ATT
Cuts at: 966 966
Size: 6500
StuI AGG'CCT
Cuts at: 289 655 1369 289
Size: 366 714 5420
Fragments arranged by size:
5420 714 366
StyI C'CwwG_G
Cuts at: 504 581 842 1145 1325 1997 2945 2969 2983
Size: 77 261 303 180 672 948 24 14
Cuts at: 2983 3052 3634 3988 504
Size: 69 582 354 3016
Fragments arranged by size:
3016 948 672 582 354 303 261 180
77 69 24 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1704 2296 2938 3365 3470 4083 4216 4675 4884
Size: 592 642 427 105 613 133 459 209
Cuts at: 4884 1704
Size: 3320
Fragments arranged by size:
3320 642 613 592 459 427 209 133
105
TatI w'GTAC_w
Cuts at: 2744 6015 2744
Size: 3271 3229
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1069 1101 1715 1747 1862 1894 2154 2186 2625
Size: 32 614 32 115 32 260 32 439
Cuts at: 2625 2657 6017 6049 1069
Size: 32 3360 32 1520
Fragments arranged by size:
3360 1520 614 439 260 115 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 412 4297 412
Size: 3885 2615
XbaI T'CTAG_A
Cuts at: 3531 3531
Size: 6500
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 856 3995 132
Size: 724 3139 2637
Fragments arranged by size:
3139 2637 724
XmaI C'CCGG_G
Cuts at: 3520 3520
Size: 6500
ZraI GAC'GTC
Cuts at: 936 2438 936
Size: 1502 4998
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AlfI AloI ApaI AscI AvrII BfrBI BmtI
BsiWI BssHII BstEII BstZ17I ClaI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PmlI PsiI PspOMI PspXI PsrI SacII SexAI SfiI
SgrAI SrfI SwaI XhoI XmnI