Restriction map for
pF1KSDA1456
(Circular) (Six-base) MAPSORT of: pF1KSDA1456.seq Check: 5486 from: 1 to: 4238
pF1KSDA1456 4238 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 1276 1276
Size: 4238
Acc65I G'GTAC_C
Cuts at: 1254 1254
Size: 4238
AclI AA'CG_TT
Cuts at: 4078 4078
Size: 4238
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2063 2495 3242 3510 2063
Size: 432 747 268 2791
Fragments arranged by size:
2791 747 432 268
AflIII A'CryG_T
Cuts at: 1590 2694 3671 1590
Size: 1104 977 2157
Fragments arranged by size:
2157 1104 977
AgeI A'CCGG_T
Cuts at: 2626 2626
Size: 4238
AlwNI CAG_nnn'CTG
Cuts at: 1543 3110 1543
Size: 1567 2671
ApaI G_GGCC'C
Cuts at: 354 354
Size: 4238
ApaLI G'TGCA_C
Cuts at: 3008 3008
Size: 4238
ApoI r'AATT_y
Cuts at: 906 1242 3591 3847 906
Size: 336 2349 256 1297
Fragments arranged by size:
2349 1297 336 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4238
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4238
AvaI C'yCGr_G
Cuts at: 1258 1258
Size: 4238
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1179 1212 1179
Size: 33 4205
BamHI G'GATC_C
Cuts at: 1263 1263
Size: 4238
BanI G'GyrC_C
Cuts at: 1254 1668 1916 1951 1254
Size: 414 248 35 3541
Fragments arranged by size:
3541 414 248 35
BanII G_rGCy'C
Cuts at: 354 1124 1157 1252 2282 354
Size: 770 33 95 1030 2310
Fragments arranged by size:
2310 1030 770 95 33
BbeI G_GCGC'C
Cuts at: 1672 1920 1672
Size: 248 3990
BciVI GTATCCnnnnn_n'
Cuts at: 403 1171 2136 2897 4238 403
Size: 768 965 761 1341 403
Fragments arranged by size:
1341 965 768 761 403
BclI T'GATC_A
Cuts at: 747 1179 1758 747
Size: 432 579 3227
Fragments arranged by size:
3227 579 432
BglII A'GATC_T
Cuts at: 625 1753 3790 625
Size: 1128 2037 1073
Fragments arranged by size:
2037 1128 1073
BlpI GC'TnA_GC
Cuts at: 1350 1350
Size: 4238
Bme1580I G_kGCm'C
Cuts at: 354 1863 1956 3012 354
Size: 1509 93 1056 1580
Fragments arranged by size:
1580 1509 1056 93
BmgBI CAC'GTC
Cuts at: 330 330
Size: 4238
BmrI ACTGGGnnnn_n'
Cuts at: 1450 1531 1866 4010 1450
Size: 81 335 2144 1678
Fragments arranged by size:
2144 1678 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2605 2605
Size: 4238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 606 606
Size: 4238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1120 1414 2293 2785 3083 1120
Size: 294 879 492 298 2275
Fragments arranged by size:
2275 879 492 298 294
BsaI GGTCTCn'nnnn_
Cuts at: 863 3942 863
Size: 3079 1159
BsaAI yAC'GTr
Cuts at: 1230 1591 1230
Size: 361 3877
BsaBI GATnn'nnATC
Cuts at: 6 968 1752 3789 6
Size: 962 784 2037 455
Fragments arranged by size:
2037 962 784 455
BsaHI Gr'CG_yC
Cuts at: 1669 1917 2619 4115 1669
Size: 248 702 1496 1792
Fragments arranged by size:
1792 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1427 1948 2626 2900 3047 3458 1427
Size: 521 678 274 147 411 2207
Fragments arranged by size:
2207 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1132 1162 1132
Size: 30 4208
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 903 903
Size: 4238
BseYI C'CCAG_C
Cuts at: 362 1663 2421 2998 3494 362
Size: 1301 758 577 496 1106
Fragments arranged by size:
1301 1106 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1780 1826 3034 104
Size: 1676 46 1208 1308
Fragments arranged by size:
1676 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 843 1124 1157 1252 2030 2220 3012 843
Size: 281 33 95 778 190 792 2069
Fragments arranged by size:
2069 792 778 281 190 95 33
Bsp1286I G_dGCh'C
Cuts at: 354 843 1124 1157 1252 1863 1956 2030 2220
Size: 489 281 33 95 611 93 74 190
Cuts at: 2220 2282 3012 354
Size: 62 730 1580
Fragments arranged by size:
1580 730 611 489 281 190 95 93
74 62 33
BspEI T'CCGG_A
Cuts at: 3458 3458
Size: 4238
BspHI T'CATG_A
Cuts at: 3414 3414
Size: 4238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 528 1288 1502 1804 2185 528
Size: 760 214 302 381 2581
Fragments arranged by size:
2581 760 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2530 2584 4068 4233 50
Size: 2480 54 1484 165 55
Fragments arranged by size:
2480 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 299 1199 2150 299
Size: 900 951 2387
Fragments arranged by size:
2387 951 900
BsrFI r'CCGG_y
Cuts at: 2236 2417 2626 2236
Size: 181 209 3848
Fragments arranged by size:
3848 209 181
BssSI C'ACGA_G
Cuts at: 2509 2867 2509
Size: 358 3880
BstAPI GCAn_nnn'nTGC
Cuts at: 330 1615 330
Size: 1285 2953
BstBI TT'CG_AA
Cuts at: 110 2599 110
Size: 2489 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 630 630
Size: 4238
BstYI r'GATC_y
Cuts at: 1 625 1053 1263 1730 1753 2088 2334 3346
Size: 624 428 210 467 23 335 246 1012
Cuts at: 3346 3432 3444 3790 1
Size: 86 12 346 449
Fragments arranged by size:
1012 624 467 449 428 346 335 246
210 86 23 12
BtgI C'CryG_G
Cuts at: 125 340 125
Size: 215 4023
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2188 2369 2532 2188
Size: 181 163 3894
Fragments arranged by size:
3894 181 163
BtsI GCAGTG_nn'
Cuts at: 409 1481 409
Size: 1072 3166
DraI TTT'AAA
Cuts at: 474 863 1237 474
Size: 389 374 3475
Fragments arranged by size:
3475 389 374
DraIII CAC_nnn'GTG
Cuts at: 507 507
Size: 4238
DrdI GACnn_nn'nnGTC
Cuts at: 734 1944 2802 734
Size: 1210 858 2170
Fragments arranged by size:
2170 1210 858
EaeI y'GGCC_r
Cuts at: 245 1823 1997 2388 2415 3760 245
Size: 1578 174 391 27 1345 723
Fragments arranged by size:
1578 1345 723 391 174 27
EagI C'GGCC_G
Cuts at: 1823 1823
Size: 4238
EarI CTCTTCn'nnn_
Cuts at: 373 391 2261 2471 373
Size: 18 1870 210 2140
Fragments arranged by size:
2140 1870 210 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2446 2756 2902 4048 2446
Size: 310 146 1146 2636
Fragments arranged by size:
2636 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1122 1155 1250 1122
Size: 33 95 4110
Fragments arranged by size:
4110 95 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 606 2063 2495 3242 3510 606
Size: 1457 432 747 268 1334
Fragments arranged by size:
1457 1334 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 350 351 1377 350
Size: 1 1026 3211
Fragments arranged by size:
3211 1026 1
EcoRI G'AATT_C
Cuts at: 1242 1242
Size: 4238
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 349 2257 349
Size: 1908 2330
FspI TGC'GCA
Cuts at: 2019 2019
Size: 4238
HaeII r_GCGC'y
Cuts at: 1672 1920 2942 3921 1672
Size: 248 1022 979 1989
Fragments arranged by size:
1989 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1027 1059 1132 1164 2080 2110 2112 2142 1027
Size: 32 73 32 916 30 2 30 3123
Fragments arranged by size:
3123 916 73 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1277 1277
Size: 4238
KasI G'GCGC_C
Cuts at: 1668 1916 1668
Size: 248 3990
KpnI G_GTAC'C
Cuts at: 1258 1258
Size: 4238
MluI A'CGCG_T
Cuts at: 3671 3671
Size: 4238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2909 3093 2909
Size: 184 4054
MscI TGG'CCA
Cuts at: 247 1999 247
Size: 1752 2486
MslI CAynn'nnrTG
Cuts at: 863 2227 2354 863
Size: 1364 127 2747
Fragments arranged by size:
2747 1364 127
MspA1I CmG'CkG
Cuts at: 1349 1663 2023 3036 3281 1349
Size: 314 360 1013 245 2306
Fragments arranged by size:
2306 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2419 2419
Size: 4238
NarI GG'CG_CC
Cuts at: 1669 1917 1669
Size: 248 3990
NcoI C'CATG_G
Cuts at: 125 340 125
Size: 215 4023
NgoMIV G'CCGG_C
Cuts at: 2417 2417
Size: 4238
NspI r_CATG'y
Cuts at: 1291 1791 2698 1291
Size: 500 907 2831
Fragments arranged by size:
2831 907 500
PciI A'CATG_T
Cuts at: 2694 2694
Size: 4238
PflMI CCAn_nnn'nTGG
Cuts at: 507 507
Size: 4238
PfoI T'CCnGG_A
Cuts at: 261 261
Size: 4238
PmeI GTTT'AAAC
Cuts at: 1237 1237
Size: 4238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3377 3409 3377
Size: 32 4206
PspOMI G'GGCC_C
Cuts at: 350 350
Size: 4238
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1152 1184 1152
Size: 32 4206
PstI C_TGCA'G
Cuts at: 1008 1129 1285 1970 1008
Size: 121 156 685 3276
Fragments arranged by size:
3276 685 156 121
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4238
PvuII CAG'CTG
Cuts at: 1663 2023 1663
Size: 360 3878
RsrII CG'GwC_CG
Cuts at: 2433 2433
Size: 4238
SacI G_AGCT'C
Cuts at: 1124 1157 1252 1124
Size: 33 95 4110
Fragments arranged by size:
4110 95 33
SalI G'TCGA_C
Cuts at: 1275 1275
Size: 4238
SapI GCTCTTCn'nnn_
Cuts at: 373 2261 2471 373
Size: 1888 210 2140
Fragments arranged by size:
2140 1888 210
SbfI CC_TGCA'GG
Cuts at: 1285 1285
Size: 4238
ScaI AGT'ACT
Cuts at: 3755 3755
Size: 4238
SfcI C'TryA_G
Cuts at: 33 1004 1125 1281 1966 2959 3150 33
Size: 971 121 156 685 993 191 1121
Fragments arranged by size:
1121 993 971 685 191 156 121
SfoI GGC'GCC
Cuts at: 1670 1918 1670
Size: 248 3990
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4238
SmaI CCC'GGG
Cuts at: 1260 1260
Size: 4238
SmlI C'TyrA_G
Cuts at: 1135 1393 2308 2800 3062 1135
Size: 258 915 492 262 2311
Fragments arranged by size:
2311 915 492 262 258
SnaBI TAC'GTA
Cuts at: 1230 1230
Size: 4238
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4238
SphI G_CATG'C
Cuts at: 1291 1791 1291
Size: 500 3738
SspI AAT'ATT
Cuts at: 549 989 549
Size: 440 3798
StyI C'CwwG_G
Cuts at: 125 340 1372 1726 125
Size: 215 1032 354 2637
Fragments arranged by size:
2637 1032 354 215
SwaI ATTT'AAAT
Cuts at: 863 863
Size: 4238
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1821 1954 2413 2622 1821
Size: 133 459 209 3437
Fragments arranged by size:
3437 459 209 133
TatI w'GTAC_w
Cuts at: 3753 3753
Size: 4238
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3755 3787 3755
Size: 32 4206
Tth111I GACn'n_nGTC
Cuts at: 2035 2035
Size: 4238
XbaI T'CTAG_A
Cuts at: 738 1269 738
Size: 531 3707
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1733 1733
Size: 4238
XmaI C'CCGG_G
Cuts at: 1258 1258
Size: 4238
XmnI GAAnn'nnTTC
Cuts at: 999 999
Size: 4238
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BbsI BbvCI BcgI BfrBI BglI BmtI
Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PpuMI PshAI PsiI PspXI SacII
SanDI SexAI SfiI SgrAI SrfI StuI XhoI ZraI