Restriction map for
pF1KSDA1470
(Circular) (Six-base) MAPSORT of: pF1KSDA1470.seq Check: 9601 from: 1 to: 4703
pF1KSDA1470 4703 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1315 1315
Size: 4703
AccI GT'mk_AC
Cuts at: 1741 1741
Size: 4703
Acc65I G'GTAC_C
Cuts at: 1719 1719
Size: 4703
AclI AA'CG_TT
Cuts at: 4543 4543
Size: 4703
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2528 2960 3707 3975 2528
Size: 432 747 268 3256
Fragments arranged by size:
3256 747 432 268
AfeI AGC'GCT
Cuts at: 247 247
Size: 4703
AflIII A'CryG_T
Cuts at: 2055 3159 4136 2055
Size: 1104 977 2622
Fragments arranged by size:
2622 1104 977
AgeI A'CCGG_T
Cuts at: 3091 3091
Size: 4703
AlwNI CAG_nnn'CTG
Cuts at: 850 1098 2008 3575 850
Size: 248 910 1567 1978
Fragments arranged by size:
1978 1567 910 248
ApaI G_GGCC'C
Cuts at: 200 312 542 200
Size: 112 230 4361
Fragments arranged by size:
4361 230 112
ApaLI G'TGCA_C
Cuts at: 3473 3473
Size: 4703
ApoI r'AATT_y
Cuts at: 528 916 1418 1707 4056 4312 528
Size: 388 502 289 2349 256 919
Fragments arranged by size:
2349 919 502 388 289 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4703
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4703
AvaI C'yCGr_G
Cuts at: 171 241 388 588 1013 1723 171
Size: 70 147 200 425 710 3151
Fragments arranged by size:
3151 710 425 200 147 70
BamHI G'GATC_C
Cuts at: 1728 1728
Size: 4703
BanI G'GyrC_C
Cuts at: 554 962 1719 2133 2381 2416 554
Size: 408 757 414 248 35 2841
Fragments arranged by size:
2841 757 414 408 248 35
BanII G_rGCy'C
Cuts at: 172 200 302 312 542 595 790 1717 2747
Size: 28 102 10 230 53 195 927 1030
Cuts at: 2747 172
Size: 2128
Fragments arranged by size:
2128 1030 927 230 195 102 53 28
10
BbeI G_GCGC'C
Cuts at: 2137 2385 2137
Size: 248 4455
BbsI GAAGACnn'nnnn_
Cuts at: 1084 1170 1529 1084
Size: 86 359 4258
Fragments arranged by size:
4258 359 86
BciVI GTATCCnnnnn_n'
Cuts at: 2601 3362 4703 2601
Size: 761 1341 2601
Fragments arranged by size:
2601 1341 761
BclI T'GATC_A
Cuts at: 2223 2223
Size: 4703
BglI GCCn_nnn'nGGC
Cuts at: 221 910 1211 221
Size: 689 301 3713
Fragments arranged by size:
3713 689 301
BglII A'GATC_T
Cuts at: 1289 2218 4255 1289
Size: 929 2037 1737
Fragments arranged by size:
2037 1737 929
BlpI GC'TnA_GC
Cuts at: 1815 1815
Size: 4703
Bme1580I G_kGCm'C
Cuts at: 200 312 542 967 1020 2328 2421 3477 200
Size: 112 230 425 53 1308 93 1056 1426
Fragments arranged by size:
1426 1308 1056 425 230 112 93 53
BmgBI CAC'GTC
Cuts at: 1128 1128
Size: 4703
BmrI ACTGGGnnnn_n'
Cuts at: 474 1519 1915 1996 2331 4475 474
Size: 1045 396 81 335 2144 702
Fragments arranged by size:
2144 1045 702 396 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 186 3070 186
Size: 2884 1819
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 1434 316
Size: 1118 3585
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1879 2758 3250 3548 1879
Size: 879 492 298 3034
Fragments arranged by size:
3034 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 591 4407 591
Size: 3816 887
BsaAI yAC'GTr
Cuts at: 1695 2056 1695
Size: 361 4342
BsaBI GATnn'nnATC
Cuts at: 6 2217 4254 6
Size: 2211 2037 455
Fragments arranged by size:
2211 2037 455
BsaHI Gr'CG_yC
Cuts at: 2134 2382 3084 4580 2134
Size: 248 702 1496 2257
Fragments arranged by size:
2257 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1892 2413 3091 3365 3512 3923 1892
Size: 521 678 274 147 411 2672
Fragments arranged by size:
2672 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 596 626 633 663 1294 1324 1594 1624 596
Size: 30 7 30 631 30 270 30 3675
Fragments arranged by size:
3675 631 270 30 30 30 30 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 175 604 175
Size: 429 4274
BseYI C'CCAG_C
Cuts at: 644 669 825 856 981 1209 1481 2128 2886
Size: 25 156 31 125 228 272 647 758
Cuts at: 2886 3463 3959 644
Size: 577 496 1388
Fragments arranged by size:
1388 758 647 577 496 272 228 156
125 31 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 587 1417 587
Size: 830 3873
BsiEI CG_ry'CG
Cuts at: 104 340 371 2245 2291 3499 104
Size: 236 31 1874 46 1208 1308
Fragments arranged by size:
1874 1308 1208 236 46 31
BsiHKAI G_wGCw'C
Cuts at: 172 302 395 1474 1717 2495 2685 3477 172
Size: 130 93 1079 243 778 190 792 1398
Fragments arranged by size:
1398 1079 792 778 243 190 130 93
BsmBI CGTCTCn'nnnn_
Cuts at: 1118 1118
Size: 4703
Bsp1286I G_dGCh'C
Cuts at: 172 200 302 312 395 542 595 790 967
Size: 28 102 10 83 147 53 195 177
Cuts at: 967 1020 1474 1717 2328 2421 2495 2685 2747
Size: 53 454 243 611 93 74 190 62
Cuts at: 2747 3477 172
Size: 730 1398
Fragments arranged by size:
1398 730 611 454 243 195 190 177
147 102 93 83 74 62 53 53
28 10
BspEI T'CCGG_A
Cuts at: 3923 3923
Size: 4703
BspHI T'CATG_A
Cuts at: 3879 3879
Size: 4703
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1315 1578 1753 1967 2269 2650 1315
Size: 263 175 214 302 381 3368
Fragments arranged by size:
3368 381 302 263 214 175
BsrBI CCG'CTC
Cuts at: 50 237 2995 3049 4533 4698 50
Size: 187 2758 54 1484 165 55
Fragments arranged by size:
2758 1484 187 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2615 2615
Size: 4703
BsrFI r'CCGG_y
Cuts at: 339 1205 2701 2882 3091 339
Size: 866 1496 181 209 1951
Fragments arranged by size:
1951 1496 866 209 181
BsrGI T'GTAC_A
Cuts at: 875 875
Size: 4703
BssHII G'CGCG_C
Cuts at: 327 327
Size: 4703
BssSI C'ACGA_G
Cuts at: 594 2974 3332 594
Size: 2380 358 1965
Fragments arranged by size:
2380 1965 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2080 2080
Size: 4703
BstBI TT'CG_AA
Cuts at: 110 3064 110
Size: 2954 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 550 550
Size: 4703
BstYI r'GATC_y
Cuts at: 1 1289 1728 2195 2218 2553 2799 3811 3897
Size: 1288 439 467 23 335 246 1012 86
Cuts at: 3897 3909 4255 1
Size: 12 346 449
Fragments arranged by size:
1288 1012 467 449 439 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 371 623 1595 371
Size: 252 972 3479
Fragments arranged by size:
3479 972 252
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 993 2653 2834 2997 993
Size: 1660 181 163 2699
Fragments arranged by size:
2699 1660 181 163
BtsI GCAGTG_nn'
Cuts at: 182 1400 1541 1946 182
Size: 1218 141 405 2939
Fragments arranged by size:
2939 1218 405 141
DraI TTT'AAA
Cuts at: 1702 1702
Size: 4703
DraIII CAC_nnn'GTG
Cuts at: 732 732
Size: 4703
DrdI GACnn_nn'nnGTC
Cuts at: 2409 3267 2409
Size: 858 3845
EaeI y'GGCC_r
Cuts at: 337 368 882 1061 1203 1457 2288 2462 2853
Size: 31 514 179 142 254 831 174 391
Cuts at: 2853 2880 4225 337
Size: 27 1345 815
Fragments arranged by size:
1345 831 815 514 391 254 179 174
142 31 27
EagI C'GGCC_G
Cuts at: 337 368 2288 337
Size: 31 1920 2752
Fragments arranged by size:
2752 1920 31
EarI CTCTTCn'nnn_
Cuts at: 1435 2726 2936 1435
Size: 1291 210 3202
Fragments arranged by size:
3202 1291 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 771 2911 3221 3367 4513 771
Size: 2140 310 146 1146 961
Fragments arranged by size:
2140 1146 961 310 146
EcoICRI GAG'CTC
Cuts at: 170 300 1715 170
Size: 130 1415 3158
Fragments arranged by size:
3158 1415 130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 1434 2528 2960 3707 3975 316
Size: 1118 1094 432 747 268 1044
Fragments arranged by size:
1118 1094 1044 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 308 309 538 539 1401 1842 308
Size: 1 229 1 862 441 3169
Fragments arranged by size:
3169 862 441 229 1 1
EcoRI G'AATT_C
Cuts at: 916 1418 1707 916
Size: 502 289 3912
Fragments arranged by size:
3912 502 289
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 457 1194 2722 457
Size: 737 1528 2438
Fragments arranged by size:
2438 1528 737
FspI TGC'GCA
Cuts at: 2484 2484
Size: 4703
HaeII r_GCGC'y
Cuts at: 249 1142 2137 2385 3407 4386 249
Size: 893 995 248 1022 979 566
Fragments arranged by size:
1022 995 979 893 566 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 633 665 1049 1081 2545 2575 2577 2607 633
Size: 32 384 32 1464 30 2 30 2729
Fragments arranged by size:
2729 1464 384 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1742 1742
Size: 4703
KasI G'GCGC_C
Cuts at: 2133 2381 2133
Size: 248 4455
KpnI G_GTAC'C
Cuts at: 1723 1723
Size: 4703
MluI A'CGCG_T
Cuts at: 4136 4136
Size: 4703
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3374 3558 3374
Size: 184 4519
MscI TGG'CCA
Cuts at: 1063 2464 1063
Size: 1401 3302
MslI CAynn'nnrTG
Cuts at: 548 1453 2692 2819 548
Size: 905 1239 127 2432
Fragments arranged by size:
2432 1239 905 127
MspA1I CmG'CkG
Cuts at: 263 278 669 825 981 1023 1481 1814 2128
Size: 15 391 156 156 42 458 333 314
Cuts at: 2128 2488 3501 3746 263
Size: 360 1013 245 1220
Fragments arranged by size:
1220 1013 458 391 360 333 314 245
156 156 42 15
NaeI GCC'GGC
Cuts at: 341 1207 2884 341
Size: 866 1677 2160
Fragments arranged by size:
2160 1677 866
NarI GG'CG_CC
Cuts at: 2134 2382 2134
Size: 248 4455
NcoI C'CATG_G
Cuts at: 1595 1595
Size: 4703
NgoMIV G'CCGG_C
Cuts at: 339 1205 2882 339
Size: 866 1677 2160
Fragments arranged by size:
2160 1677 866
NspI r_CATG'y
Cuts at: 752 1756 2256 3163 752
Size: 1004 500 907 2292
Fragments arranged by size:
2292 1004 907 500
PasI CC'CwG_GG
Cuts at: 1270 1270
Size: 4703
PciI A'CATG_T
Cuts at: 3159 3159
Size: 4703
PflMI CCAn_nnn'nTGG
Cuts at: 768 1152 1481 768
Size: 384 329 3990
Fragments arranged by size:
3990 384 329
PfoI T'CCnGG_A
Cuts at: 1157 1157
Size: 4703
PmeI GTTT'AAAC
Cuts at: 1702 1702
Size: 4703
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3842 3874 3842
Size: 32 4671
PpuMI rG'GwC_Cy
Cuts at: 1401 1401
Size: 4703
PshAI GACnn'nnGTC
Cuts at: 583 583
Size: 4703
PspOMI G'GGCC_C
Cuts at: 196 308 538 196
Size: 112 230 4361
Fragments arranged by size:
4361 230 112
PstI C_TGCA'G
Cuts at: 253 747 1548 1750 2435 253
Size: 494 801 202 685 2521
Fragments arranged by size:
2521 801 685 494 202
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4703
PvuII CAG'CTG
Cuts at: 669 825 981 1481 2128 2488 669
Size: 156 156 500 647 360 2884
Fragments arranged by size:
2884 647 500 360 156 156
RsrII CG'GwC_CG
Cuts at: 2898 2898
Size: 4703
SacI G_AGCT'C
Cuts at: 172 302 1717 172
Size: 130 1415 3158
Fragments arranged by size:
3158 1415 130
SalI G'TCGA_C
Cuts at: 1740 1740
Size: 4703
SapI GCTCTTCn'nnn_
Cuts at: 1435 2726 2936 1435
Size: 1291 210 3202
Fragments arranged by size:
3202 1291 210
SbfI CC_TGCA'GG
Cuts at: 1750 1750
Size: 4703
ScaI AGT'ACT
Cuts at: 4220 4220
Size: 4703
SfcI C'TryA_G
Cuts at: 33 249 743 1544 1746 2431 3424 3615 33
Size: 216 494 801 202 685 993 191 1121
Fragments arranged by size:
1121 993 801 685 494 216 202 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1211 1211
Size: 4703
SfoI GGC'GCC
Cuts at: 2135 2383 2135
Size: 248 4455
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4703
SmaI CCC'GGG
Cuts at: 1015 1725 1015
Size: 710 3993
SmlI C'TyrA_G
Cuts at: 1858 2773 3265 3527 1858
Size: 915 492 262 3034
Fragments arranged by size:
3034 915 492 262
SnaBI TAC'GTA
Cuts at: 1695 1695
Size: 4703
SpeI A'CTAG_T
Cuts at: 67 1044 67
Size: 977 3726
SphI G_CATG'C
Cuts at: 1756 2256 1756
Size: 500 4203
SrfI GCCC'GGGC
Cuts at: 1015 1015
Size: 4703
StyI C'CwwG_G
Cuts at: 398 767 831 1595 1613 1837 2191 398
Size: 369 64 764 18 224 354 2910
Fragments arranged by size:
2910 764 369 354 224 64 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 511 542 950 1321 2286 2419 2878 3087 511
Size: 31 408 371 965 133 459 209 2127
Fragments arranged by size:
2127 965 459 408 371 209 133 31
TatI w'GTAC_w
Cuts at: 875 4218 875
Size: 3343 1360
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 596 628 4220 4252 596
Size: 32 3592 32 1047
Fragments arranged by size:
3592 1047 32 32
Tth111I GACn'n_nGTC
Cuts at: 2500 2500
Size: 4703
XbaI T'CTAG_A
Cuts at: 1734 1734
Size: 4703
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1294 2198 1294
Size: 904 3799
XmaI C'CCGG_G
Cuts at: 1013 1723 1013
Size: 710 3993
XmnI GAAnn'nnTTC
Cuts at: 1046 1046
Size: 4703
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SrfI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmI
BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PsiI PspXI PsrI SacII SanDI
SexAI SgrAI SspI StuI SwaI XhoI ZraI