Restriction map for
pF1KSDA1473
(Circular) (Six-base) MAPSORT of: pF1KSDA1473.seq Check: 9577 from: 1 to: 4730
pF1KSDA1473 4730 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 1768 1768
Size: 4730
Acc65I G'GTAC_C
Cuts at: 1746 1746
Size: 4730
AclI AA'CG_TT
Cuts at: 4570 4570
Size: 4730
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2555 2987 3734 4002 2555
Size: 432 747 268 3283
Fragments arranged by size:
3283 747 432 268
AflIII A'CryG_T
Cuts at: 2082 3186 4163 2082
Size: 1104 977 2649
Fragments arranged by size:
2649 1104 977
AgeI A'CCGG_T
Cuts at: 3118 3118
Size: 4730
AlwNI CAG_nnn'CTG
Cuts at: 2035 3602 2035
Size: 1567 3163
ApaLI G'TGCA_C
Cuts at: 403 3500 403
Size: 3097 1633
ApoI r'AATT_y
Cuts at: 357 496 504 608 692 860 1045 1112 1196
Size: 139 8 104 84 168 185 67 84
Cuts at: 1196 1213 1280 1734 4083 4339 357
Size: 17 67 454 2349 256 748
Fragments arranged by size:
2349 748 454 256 185 168 139 104
84 84 67 67 17 8
AseI AT'TA_AT
Cuts at: 20 20
Size: 4730
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4730
AvaI C'yCGr_G
Cuts at: 275 1750 275
Size: 1475 3255
BamHI G'GATC_C
Cuts at: 1755 1755
Size: 4730
BanI G'GyrC_C
Cuts at: 1746 2160 2408 2443 1746
Size: 414 248 35 4033
Fragments arranged by size:
4033 414 248 35
BanII G_rGCy'C
Cuts at: 1744 2774 1744
Size: 1030 3700
BbeI G_GCGC'C
Cuts at: 2164 2412 2164
Size: 248 4482
BbsI GAAGACnn'nnnn_
Cuts at: 144 144
Size: 4730
BciVI GTATCCnnnnn_n'
Cuts at: 2628 3389 4730 2628
Size: 761 1341 2628
Fragments arranged by size:
2628 1341 761
BclI T'GATC_A
Cuts at: 185 2250 185
Size: 2065 2665
BglII A'GATC_T
Cuts at: 2245 4282 2245
Size: 2037 2693
BlpI GC'TnA_GC
Cuts at: 1842 1842
Size: 4730
Bme1580I G_kGCm'C
Cuts at: 407 2355 2448 3504 407
Size: 1948 93 1056 1633
Fragments arranged by size:
1948 1633 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 247 1942 2023 2358 4502 247
Size: 1695 81 335 2144 475
Fragments arranged by size:
2144 1695 475 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1651 3097 1651
Size: 1446 3284
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 890 974 1058 1142 1226 1310 1394 1478
Size: 673 84 84 84 84 84 84 84
Cuts at: 1478 1562 1646 217
Size: 84 84 3301
Fragments arranged by size:
3301 673 84 84 84 84 84 84
84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1906 2785 3277 3575 1906
Size: 879 492 298 3061
Fragments arranged by size:
3061 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 272 658 4434 272
Size: 386 3776 568
Fragments arranged by size:
3776 568 386
BsaAI yAC'GTr
Cuts at: 1722 2083 1722
Size: 361 4369
BsaBI GATnn'nnATC
Cuts at: 6 2244 4281 6
Size: 2238 2037 455
Fragments arranged by size:
2238 2037 455
BsaHI Gr'CG_yC
Cuts at: 2161 2409 3111 4607 2161
Size: 248 702 1496 2284
Fragments arranged by size:
2284 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1919 2440 3118 3392 3539 3950 1919
Size: 521 678 274 147 411 2699
Fragments arranged by size:
2699 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 823 1581 823
Size: 758 3972
BseYI C'CCAG_C
Cuts at: 2155 2913 3490 3986 2155
Size: 758 577 496 2899
Fragments arranged by size:
2899 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 917 917
Size: 4730
BsiEI CG_ry'CG
Cuts at: 104 2272 2318 3526 104
Size: 2168 46 1208 1308
Fragments arranged by size:
2168 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 407 1744 2522 2712 3504 407
Size: 1337 778 190 792 1633
Fragments arranged by size:
1633 1337 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 407 1744 2355 2448 2522 2712 2774 3504 407
Size: 1337 611 93 74 190 62 730 1633
Fragments arranged by size:
1633 1337 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3950 3950
Size: 4730
BspHI T'CATG_A
Cuts at: 3906 3906
Size: 4730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1780 1994 2296 2677 1780
Size: 214 302 381 3833
Fragments arranged by size:
3833 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3022 3076 4560 4725 50
Size: 2972 54 1484 165 55
Fragments arranged by size:
2972 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1673 2642 1673
Size: 969 3761
BsrFI r'CCGG_y
Cuts at: 746 1250 2728 2909 3118 746
Size: 504 1478 181 209 2358
Fragments arranged by size:
2358 1478 504 209 181
BssSI C'ACGA_G
Cuts at: 3001 3359 3001
Size: 358 4372
BstAPI GCAn_nnn'nTGC
Cuts at: 2107 2107
Size: 4730
BstBI TT'CG_AA
Cuts at: 110 3091 110
Size: 2981 1749
BstYI r'GATC_y
Cuts at: 1 1755 2222 2245 2580 2826 3838 3924 3936
Size: 1754 467 23 335 246 1012 86 12
Cuts at: 3936 4282 1
Size: 346 449
Fragments arranged by size:
1754 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2680 2861 3024 2680
Size: 181 163 4386
Fragments arranged by size:
4386 181 163
BtsI GCAGTG_nn'
Cuts at: 1973 1973
Size: 4730
DraI TTT'AAA
Cuts at: 1164 1729 1164
Size: 565 4165
DrdI GACnn_nn'nnGTC
Cuts at: 2436 3294 2436
Size: 858 3872
EaeI y'GGCC_r
Cuts at: 286 2315 2489 2880 2907 4252 286
Size: 2029 174 391 27 1345 764
Fragments arranged by size:
2029 1345 764 391 174 27
EagI C'GGCC_G
Cuts at: 2315 2315
Size: 4730
EarI CTCTTCn'nnn_
Cuts at: 220 719 2753 2963 220
Size: 499 2034 210 1987
Fragments arranged by size:
2034 1987 499 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2938 3248 3394 4540 2938
Size: 310 146 1146 3128
Fragments arranged by size:
3128 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1742 1742
Size: 4730
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 890 974 1058 1142 1226 1310 1394 1478
Size: 673 84 84 84 84 84 84 84
Cuts at: 1478 1562 1646 2555 2987 3734 4002 217
Size: 84 84 909 432 747 268 945
Fragments arranged by size:
945 909 747 673 432 268 84 84
84 84 84 84 84 84 84
EcoO109I rG'GnC_Cy
Cuts at: 1869 1869
Size: 4730
EcoRI G'AATT_C
Cuts at: 608 692 860 1112 1196 1280 1734 608
Size: 84 168 252 84 84 454 3604
Fragments arranged by size:
3604 454 252 168 84 84 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2749 2749
Size: 4730
FspI TGC'GCA
Cuts at: 2511 2511
Size: 4730
HaeII r_GCGC'y
Cuts at: 2164 2412 3434 4413 2164
Size: 248 1022 979 2481
Fragments arranged by size:
2481 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 177 209 2572 2602 2604 2634 177
Size: 32 2363 30 2 30 2273
Fragments arranged by size:
2363 2273 32 30 30 2
HincII GTy'rAC
Cuts at: 1769 1769
Size: 4730
HindIII A'AGCT_T
Cuts at: 821 989 1157 1325 1409 1493 821
Size: 168 168 168 84 84 4058
Fragments arranged by size:
4058 168 168 168 84 84
KasI G'GCGC_C
Cuts at: 2160 2408 2160
Size: 248 4482
KpnI G_GTAC'C
Cuts at: 1750 1750
Size: 4730
MluI A'CGCG_T
Cuts at: 4163 4163
Size: 4730
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3401 3585 3401
Size: 184 4546
MscI TGG'CCA
Cuts at: 288 2491 288
Size: 2203 2527
MslI CAynn'nnrTG
Cuts at: 2719 2846 2719
Size: 127 4603
MspA1I CmG'CkG
Cuts at: 1841 2155 2515 3528 3773 1841
Size: 314 360 1013 245 2798
Fragments arranged by size:
2798 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2911 2911
Size: 4730
NarI GG'CG_CC
Cuts at: 2161 2409 2161
Size: 248 4482
NgoMIV G'CCGG_C
Cuts at: 2909 2909
Size: 4730
NspI r_CATG'y
Cuts at: 582 1783 2283 3190 582
Size: 1201 500 907 2122
Fragments arranged by size:
2122 1201 907 500
PciI A'CATG_T
Cuts at: 3186 3186
Size: 4730
PmeI GTTT'AAAC
Cuts at: 1729 1729
Size: 4730
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3869 3901 3869
Size: 32 4698
PstI C_TGCA'G
Cuts at: 1777 2462 1777
Size: 685 4045
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4730
PvuII CAG'CTG
Cuts at: 2155 2515 2155
Size: 360 4370
RsrII CG'GwC_CG
Cuts at: 2925 2925
Size: 4730
SacI G_AGCT'C
Cuts at: 1744 1744
Size: 4730
SalI G'TCGA_C
Cuts at: 1767 1767
Size: 4730
SapI GCTCTTCn'nnn_
Cuts at: 2753 2963 2753
Size: 210 4520
SbfI CC_TGCA'GG
Cuts at: 1777 1777
Size: 4730
ScaI AGT'ACT
Cuts at: 4247 4247
Size: 4730
SexAI A'CCwGG_T
Cuts at: 146 146
Size: 4730
SfcI C'TryA_G
Cuts at: 33 138 1773 2458 3451 3642 33
Size: 105 1635 685 993 191 1121
Fragments arranged by size:
1635 1121 993 685 191 105
SfoI GGC'GCC
Cuts at: 2162 2410 2162
Size: 248 4482
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4730
SmaI CCC'GGG
Cuts at: 1752 1752
Size: 4730
SmlI C'TyrA_G
Cuts at: 1885 2800 3292 3554 1885
Size: 915 492 262 3061
Fragments arranged by size:
3061 915 492 262
SnaBI TAC'GTA
Cuts at: 1722 1722
Size: 4730
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4730
SphI G_CATG'C
Cuts at: 582 1783 2283 582
Size: 1201 500 3029
Fragments arranged by size:
3029 1201 500
SspI AAT'ATT
Cuts at: 461 461
Size: 4730
StuI AGG'CCT
Cuts at: 1075 1075
Size: 4730
StyI C'CwwG_G
Cuts at: 214 1864 2218 214
Size: 1650 354 2726
Fragments arranged by size:
2726 1650 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2313 2446 2905 3114 2313
Size: 133 459 209 3929
Fragments arranged by size:
3929 459 209 133
TatI w'GTAC_w
Cuts at: 4245 4245
Size: 4730
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4247 4279 4247
Size: 32 4698
Tth111I GACn'n_nGTC
Cuts at: 2527 2527
Size: 4730
XbaI T'CTAG_A
Cuts at: 1761 1761
Size: 4730
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2225 2225
Size: 4730
XmaI C'CCGG_G
Cuts at: 1750 1750
Size: 4730
XmnI GAAnn'nnTTC
Cuts at: 544 1116 544
Size: 572 4158
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SrfI SwaI XhoI ZraI