Restriction map for
pF1KSDA1486
(Circular) (Six-base) MAPSORT of: pF1KSDA1486.seq Check: 4608 from: 1 to: 5096
pF1KSDA1486 5096 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AatII G_ACGT'C
Cuts at: 787 1996 787
Size: 1209 3887
AccI GT'mk_AC
Cuts at: 926 1378 1899 1990 2134 926
Size: 452 521 91 144 3888
Fragments arranged by size:
3888 521 452 144 91
Acc65I G'GTAC_C
Cuts at: 2058 2112 2058
Size: 54 5042
AclI AA'CG_TT
Cuts at: 4936 4936
Size: 5096
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 665 876 1520 2921 3353 4100 4368 665
Size: 211 644 1401 432 747 268 1393
Fragments arranged by size:
1401 1393 747 644 432 268 211
AflIII A'CryG_T
Cuts at: 1229 2448 3552 4529 1229
Size: 1219 1104 977 1796
Fragments arranged by size:
1796 1219 1104 977
AgeI A'CCGG_T
Cuts at: 3484 3484
Size: 5096
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1714 2059 1714
Size: 345 4751
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1383 1415 1748 1780 1383
Size: 32 333 32 4699
Fragments arranged by size:
4699 333 32 32
AlwNI CAG_nnn'CTG
Cuts at: 486 521 2401 3968 486
Size: 35 1880 1567 1614
Fragments arranged by size:
1880 1614 1567 35
ApaI G_GGCC'C
Cuts at: 776 776
Size: 5096
ApaLI G'TGCA_C
Cuts at: 3866 3866
Size: 5096
ApoI r'AATT_y
Cuts at: 2100 4449 4705 2100
Size: 2349 256 2491
Fragments arranged by size:
2491 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5096
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5096
AvaI C'yCGr_G
Cuts at: 254 838 1620 2116 254
Size: 584 782 496 3234
Fragments arranged by size:
3234 782 584 496
BamHI G'GATC_C
Cuts at: 2121 2121
Size: 5096
BanI G'GyrC_C
Cuts at: 1026 2058 2112 2526 2774 2809 1026
Size: 1032 54 414 248 35 3313
Fragments arranged by size:
3313 1032 414 248 54 35
BanII G_rGCy'C
Cuts at: 776 854 1531 1654 1924 2110 3140 776
Size: 78 677 123 270 186 1030 2732
Fragments arranged by size:
2732 1030 677 270 186 123 78
BbeI G_GCGC'C
Cuts at: 2530 2778 2530
Size: 248 4848
BbsI GAAGACnn'nnnn_
Cuts at: 170 330 1823 170
Size: 160 1493 3443
Fragments arranged by size:
3443 1493 160
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 726 760 726
Size: 34 5062
BciVI GTATCCnnnnn_n'
Cuts at: 2994 3755 5096 2994
Size: 761 1341 2994
Fragments arranged by size:
2994 1341 761
BclI T'GATC_A
Cuts at: 2616 2616
Size: 5096
BglII A'GATC_T
Cuts at: 2611 4648 2611
Size: 2037 3059
BlpI GC'TnA_GC
Cuts at: 577 733 2208 577
Size: 156 1475 3465
Fragments arranged by size:
3465 1475 156
Bme1580I G_kGCm'C
Cuts at: 505 776 2721 2814 3870 505
Size: 271 1945 93 1056 1731
Fragments arranged by size:
1945 1731 1056 271 93
BmgBI CAC'GTC
Cuts at: 1284 1284
Size: 5096
BmrI ACTGGGnnnn_n'
Cuts at: 1389 2308 2389 2724 4868 1389
Size: 919 81 335 2144 1617
Fragments arranged by size:
2144 1617 919 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3463 3463
Size: 5096
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 441 1219 1324 1678 1821 169
Size: 272 778 105 354 143 3444
Fragments arranged by size:
3444 778 354 272 143 105
Bpu10I CC'TnA_GC
Cuts at: 1471 1856 1471
Size: 385 4711
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 291 1506 2272 3151 3643 3941 291
Size: 1215 766 879 492 298 1446
Fragments arranged by size:
1446 1215 879 766 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 827 4800 827
Size: 3973 1123
BsaAI yAC'GTr
Cuts at: 387 2088 2449 387
Size: 1701 361 3034
Fragments arranged by size:
3034 1701 361
BsaBI GATnn'nnATC
Cuts at: 6 2610 4647 6
Size: 2604 2037 455
Fragments arranged by size:
2604 2037 455
BsaHI Gr'CG_yC
Cuts at: 784 971 1258 1828 1993 2527 2775 3477 4973
Size: 187 287 570 165 534 248 702 1496
Cuts at: 4973 784
Size: 907
Fragments arranged by size:
1496 907 702 570 534 287 248 187
165
BsaWI w'CCGG_w
Cuts at: 2285 2806 3484 3758 3905 4316 2285
Size: 521 678 274 147 411 3065
Fragments arranged by size:
3065 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 841 871 1383 1413 841
Size: 30 512 30 4524
Fragments arranged by size:
4524 512 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 419 689 792 862 1347 1671 1675 1997 419
Size: 270 103 70 485 324 4 322 3518
Fragments arranged by size:
3518 485 324 322 270 103 70 4
BseYI C'CCAG_C
Cuts at: 565 2521 3279 3856 4352 565
Size: 1956 758 577 496 1309
Fragments arranged by size:
1956 1309 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 472
Size: 5096
BsiEI CG_ry'CG
Cuts at: 104 1689 2638 2684 3892 104
Size: 1585 949 46 1208 1308
Fragments arranged by size:
1585 1308 1208 949 46
BsiHKAI G_wGCw'C
Cuts at: 583 739 1531 2110 2888 3078 3870 583
Size: 156 792 579 778 190 792 1809
Fragments arranged by size:
1809 792 792 778 579 190 156
BsmI GAATG_Cn'
Cuts at: 243 1506 243
Size: 1263 3833
Bsp1286I G_dGCh'C
Cuts at: 505 583 739 776 854 1531 1654 1924 2110
Size: 78 156 37 78 677 123 270 186
Cuts at: 2110 2721 2814 2888 3078 3140 3870 505
Size: 611 93 74 190 62 730 1731
Fragments arranged by size:
1731 730 677 611 270 190 186 156
123 93 78 78 74 62 37
BspEI T'CCGG_A
Cuts at: 4316 4316
Size: 5096
BspHI T'CATG_A
Cuts at: 413 4272 413
Size: 3859 1237
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1118 1341 2051 2146 2360 2662 3043 1118
Size: 223 710 95 214 302 381 3171
Fragments arranged by size:
3171 710 381 302 223 214 95
BsrBI CCG'CTC
Cuts at: 50 623 3388 3442 4926 5091 50
Size: 573 2765 54 1484 165 55
Fragments arranged by size:
2765 1484 573 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1158 3008 1158
Size: 1850 3246
BsrFI r'CCGG_y
Cuts at: 1718 1979 3094 3275 3484 1718
Size: 261 1115 181 209 3330
Fragments arranged by size:
3330 1115 261 209 181
BsrGI T'GTAC_A
Cuts at: 857 857
Size: 5096
BssSI C'ACGA_G
Cuts at: 1647 3367 3725 1647
Size: 1720 358 3018
Fragments arranged by size:
3018 1720 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2473 2473
Size: 5096
BstBI TT'CG_AA
Cuts at: 110 3457 110
Size: 3347 1749
BstEII G'GTnAC_C
Cuts at: 1890 1890
Size: 5096
BstYI r'GATC_y
Cuts at: 1 2121 2588 2611 2946 3192 4204 4290 4302
Size: 2120 467 23 335 246 1012 86 12
Cuts at: 4302 4648 1
Size: 346 449
Fragments arranged by size:
2120 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 158 431 2026 158
Size: 273 1595 3228
Fragments arranged by size:
3228 1595 273
BtgI C'CryG_G
Cuts at: 793 827 1022 1319 1404 1595 1821 793
Size: 34 195 297 85 191 226 4068
Fragments arranged by size:
4068 297 226 195 191 85 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 689 3046 3227 3390 689
Size: 2357 181 163 2395
Fragments arranged by size:
2395 2357 181 163
BtsI GCAGTG_nn'
Cuts at: 605 1015 2043 2339 605
Size: 410 1028 296 3362
Fragments arranged by size:
3362 1028 410 296
DraI TTT'AAA
Cuts at: 2095 2095
Size: 5096
DrdI GACnn_nn'nnGTC
Cuts at: 648 2802 3660 648
Size: 2154 858 2084
Fragments arranged by size:
2154 2084 858
EaeI y'GGCC_r
Cuts at: 1290 1686 1716 2681 2855 3246 3273 4618 1290
Size: 396 30 965 174 391 27 1345 1768
Fragments arranged by size:
1768 1345 965 396 391 174 30 27
EagI C'GGCC_G
Cuts at: 1686 2681 1686
Size: 995 4101
EarI CTCTTCn'nnn_
Cuts at: 839 1681 3119 3329 839
Size: 842 1438 210 2606
Fragments arranged by size:
2606 1438 842 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1085 3304 3614 3760 4906 1085
Size: 2219 310 146 1146 1275
Fragments arranged by size:
2219 1275 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1529 2108 1529
Size: 579 4517
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 441 665 876 1219 1324 1520 1678 1821
Size: 272 224 211 343 105 196 158 143
Cuts at: 1821 2921 3353 4100 4368 169
Size: 1100 432 747 268 897
Fragments arranged by size:
1100 897 747 432 343 272 268 224
211 196 158 143 105
EcoO109I rG'GnC_Cy
Cuts at: 561 773 1330 1457 2235 561
Size: 212 557 127 778 3422
Fragments arranged by size:
3422 778 557 212 127
EcoRI G'AATT_C
Cuts at: 2100 2100
Size: 5096
EcoRV GAT'ATC
Cuts at: 131 131
Size: 5096
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3115 3115
Size: 5096
FspI TGC'GCA
Cuts at: 1702 2877 1702
Size: 1175 3921
HaeII r_GCGC'y
Cuts at: 1725 2530 2778 3800 4779 1725
Size: 805 248 1022 979 2042
Fragments arranged by size:
2042 1022 979 805 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 138 170 684 716 1832 1864 2938 2968 2970
Size: 32 514 32 1116 32 1074 30 2
Cuts at: 2970 3000 138
Size: 30 2234
Fragments arranged by size:
2234 1116 1074 514 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1590 1900 1991 2135 1590
Size: 310 91 144 4551
Fragments arranged by size:
4551 310 144 91
HindIII A'AGCT_T
Cuts at: 1210 1210
Size: 5096
KasI G'GCGC_C
Cuts at: 2526 2774 2526
Size: 248 4848
KpnI G_GTAC'C
Cuts at: 2062 2116 2062
Size: 54 5042
MluI A'CGCG_T
Cuts at: 4529 4529
Size: 5096
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1199 1778 3767 3951 1199
Size: 579 1989 184 2344
Fragments arranged by size:
2344 1989 579 184
MscI TGG'CCA
Cuts at: 2857 2857
Size: 5096
MslI CAynn'nnrTG
Cuts at: 418 674 865 3085 3212 418
Size: 256 191 2220 127 2302
Fragments arranged by size:
2302 2220 256 191 127
MspA1I CmG'CkG
Cuts at: 521 732 795 829 998 1131 1321 1597 1656
Size: 211 63 34 169 133 190 276 59
Cuts at: 1656 2207 2521 2881 3894 4139 521
Size: 551 314 360 1013 245 1478
Fragments arranged by size:
1478 1013 551 360 314 276 245 211
190 169 133 63 59 34
NaeI GCC'GGC
Cuts at: 1720 3277 1720
Size: 1557 3539
NarI GG'CG_CC
Cuts at: 2527 2775 2527
Size: 248 4848
NcoI C'CATG_G
Cuts at: 1404 1821 1404
Size: 417 4679
NgoMIV G'CCGG_C
Cuts at: 1718 3275 1718
Size: 1557 3539
NspI r_CATG'y
Cuts at: 2149 2649 3556 2149
Size: 500 907 3689
Fragments arranged by size:
3689 907 500
PasI CC'CwG_GG
Cuts at: 1326 1326
Size: 5096
PciI A'CATG_T
Cuts at: 3552 3552
Size: 5096
PfoI T'CCnGG_A
Cuts at: 1636 1636
Size: 5096
PmeI GTTT'AAAC
Cuts at: 2095 2095
Size: 5096
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1383 1415 4235 4267 1383
Size: 32 2820 32 2212
Fragments arranged by size:
2820 2212 32 32
PpuMI rG'GwC_Cy
Cuts at: 561 1330 1457 561
Size: 769 127 4200
Fragments arranged by size:
4200 769 127
PshAI GACnn'nnGTC
Cuts at: 1563 1563
Size: 5096
PspOMI G'GGCC_C
Cuts at: 772 772
Size: 5096
PstI C_TGCA'G
Cuts at: 612 1740 2143 2828 612
Size: 1128 403 685 2880
Fragments arranged by size:
2880 1128 685 403
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5096
PvuII CAG'CTG
Cuts at: 521 732 998 2521 2881 521
Size: 211 266 1523 360 2736
Fragments arranged by size:
2736 1523 360 266 211
RsrII CG'GwC_CG
Cuts at: 3291 3291
Size: 5096
SacI G_AGCT'C
Cuts at: 1531 2110 1531
Size: 579 4517
SacII CC_GC'GG
Cuts at: 796 830 1322 1598 796
Size: 34 492 276 4294
Fragments arranged by size:
4294 492 276 34
SalI G'TCGA_C
Cuts at: 1898 1989 2133 1898
Size: 91 144 4861
Fragments arranged by size:
4861 144 91
SanDI GG'GwC_CC
Cuts at: 561 561
Size: 5096
SapI GCTCTTCn'nnn_
Cuts at: 3119 3329 3119
Size: 210 4886
SbfI CC_TGCA'GG
Cuts at: 2143 2143
Size: 5096
ScaI AGT'ACT
Cuts at: 4613 4613
Size: 5096
SfcI C'TryA_G
Cuts at: 33 608 1736 2139 2824 3817 4008 33
Size: 575 1128 403 685 993 191 1121
Fragments arranged by size:
1128 1121 993 685 575 403 191
SfoI GGC'GCC
Cuts at: 2528 2776 2528
Size: 248 4848
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5096
SmaI CCC'GGG
Cuts at: 1622 2118 1622
Size: 496 4600
SmlI C'TyrA_G
Cuts at: 254 270 1521 2251 3166 3658 3920 254
Size: 16 1251 730 915 492 262 1430
Fragments arranged by size:
1430 1251 915 730 492 262 16
SnaBI TAC'GTA
Cuts at: 2088 2088
Size: 5096
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5096
SphI G_CATG'C
Cuts at: 2149 2649 2149
Size: 500 4596
SspI AAT'ATT
Cuts at: 682 682
Size: 5096
StuI AGG'CCT
Cuts at: 214 1051 1485 214
Size: 837 434 3825
Fragments arranged by size:
3825 837 434
StyI C'CwwG_G
Cuts at: 1046 1070 1404 1821 2230 2584 1046
Size: 24 334 417 409 354 3558
Fragments arranged by size:
3558 417 409 354 334 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 561 1659 2679 2812 3271 3480 561
Size: 1098 1020 133 459 209 2177
Fragments arranged by size:
2177 1098 1020 459 209 133
TatI w'GTAC_w
Cuts at: 188 857 4611 188
Size: 669 3754 673
Fragments arranged by size:
3754 673 669
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 371 403 1197 1229 4613 4645 371
Size: 32 794 32 3384 32 822
Fragments arranged by size:
3384 822 794 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2893 2893
Size: 5096
XbaI T'CTAG_A
Cuts at: 2127 2127
Size: 5096
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1402 1653 2591 1402
Size: 251 938 3907
Fragments arranged by size:
3907 938 251
XhoI C'TCGA_G
Cuts at: 254 254
Size: 5096
XmaI C'CCGG_G
Cuts at: 1620 2116 1620
Size: 496 4600
ZraI GAC'GTC
Cuts at: 785 1994 785
Size: 1209 3887
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PfoI PmeI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AscI AvrII BaeI
BbvCI BfrBI BglI BmtI BsiWI BsmBI BssHII BstXI
BstZ17I ClaI CspCI DraIII EcoNI FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PmlI PsiI PspXI PsrI SexAI SfiI SgrAI SrfI
SwaI XmnI