Restriction map for
pF1KSDA1490
(Circular) (Six-base) MAPSORT of: pF1KSDA1490.seq Check: 3071 from: 1 to: 5381
pF1KSDA1490 5381 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2329 2329
Size: 5381
AccI GT'mk_AC
Cuts at: 2419 2419
Size: 5381
Acc65I G'GTAC_C
Cuts at: 2397 2397
Size: 5381
AclI AA'CG_TT
Cuts at: 5221 5221
Size: 5381
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 701 705 985 3206 3638 4385 4653 174
Size: 527 4 280 2221 432 747 268 902
Fragments arranged by size:
2221 902 747 527 432 280 268 4
AflIII A'CryG_T
Cuts at: 2009 2056 2733 3837 4814 2009
Size: 47 677 1104 977 2576
Fragments arranged by size:
2576 1104 977 677 47
AgeI A'CCGG_T
Cuts at: 3769 3769
Size: 5381
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1630 1630
Size: 5381
AlwNI CAG_nnn'CTG
Cuts at: 136 2686 4253 136
Size: 2550 1567 1264
Fragments arranged by size:
2550 1567 1264
ApaLI G'TGCA_C
Cuts at: 4151 4151
Size: 5381
ApoI r'AATT_y
Cuts at: 685 1073 2385 4734 4990 685
Size: 388 1312 2349 256 1076
Fragments arranged by size:
2349 1312 1076 388 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5381
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5381
AvaI C'yCGr_G
Cuts at: 2401 2401
Size: 5381
BamHI G'GATC_C
Cuts at: 1583 1845 2406 1583
Size: 262 561 4558
Fragments arranged by size:
4558 561 262
BanI G'GyrC_C
Cuts at: 406 513 2341 2397 2811 3059 3094 406
Size: 107 1828 56 414 248 35 2693
Fragments arranged by size:
2693 1828 414 248 107 56 35
BanII G_rGCy'C
Cuts at: 447 569 1199 2395 3425 447
Size: 122 630 1196 1030 2403
Fragments arranged by size:
2403 1196 1030 630 122
BbeI G_GCGC'C
Cuts at: 2815 3063 2815
Size: 248 5133
BbvCI CC'TCA_GC
Cuts at: 1190 1190
Size: 5381
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 817 851 817
Size: 34 5347
BciVI GTATCCnnnnn_n'
Cuts at: 3279 4040 5381 3279
Size: 761 1341 3279
Fragments arranged by size:
3279 1341 761
BclI T'GATC_A
Cuts at: 2901 2901
Size: 5381
BfrBI ATG'CAT
Cuts at: 1103 1258 1378 1103
Size: 155 120 5106
Fragments arranged by size:
5106 155 120
BglI GCCn_nnn'nGGC
Cuts at: 1811 1811
Size: 5381
BglII A'GATC_T
Cuts at: 2896 4933 2896
Size: 2037 3344
BlpI GC'TnA_GC
Cuts at: 710 2493 710
Size: 1783 3598
Bme1580I G_kGCm'C
Cuts at: 460 3006 3099 4155 460
Size: 2546 93 1056 1686
Fragments arranged by size:
2546 1686 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 279 2593 2674 3009 5153 279
Size: 2314 81 335 2144 507
Fragments arranged by size:
2314 2144 507 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3748 3748
Size: 5381
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 654 1836 654
Size: 1182 4199
Bpu10I CC'TnA_GC
Cuts at: 1190 1306 1190
Size: 116 5265
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1079 2557 3436 3928 4226 1079
Size: 1478 879 492 298 2234
Fragments arranged by size:
2234 1478 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5085 5085
Size: 5381
BsaAI yAC'GTr
Cuts at: 489 2373 2734 489
Size: 1884 361 3136
Fragments arranged by size:
3136 1884 361
BsaBI GATnn'nnATC
Cuts at: 6 2895 4932 6
Size: 2889 2037 455
Fragments arranged by size:
2889 2037 455
BsaHI Gr'CG_yC
Cuts at: 2812 3060 3762 5258 2812
Size: 248 702 1496 2935
Fragments arranged by size:
2935 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2570 3091 3769 4043 4190 4601 2570
Size: 521 678 274 147 411 3350
Fragments arranged by size:
3350 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2025 2055 2325 2355 2025
Size: 30 270 30 5051
Fragments arranged by size:
5051 270 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 336 372 517 520 892 1207 2046 336
Size: 36 145 3 372 315 839 3671
Fragments arranged by size:
3671 839 372 315 145 36 3
BseYI C'CCAG_C
Cuts at: 277 2806 3564 4141 4637 277
Size: 2529 758 577 496 1021
Fragments arranged by size:
2529 1021 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 782 782
Size: 5381
BsiEI CG_ry'CG
Cuts at: 104 2923 2969 4177 104
Size: 2819 46 1208 1308
Fragments arranged by size:
2819 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 269 325 569 1199 1900 2019 2395 3173 3363
Size: 56 244 630 701 119 376 778 190
Cuts at: 3363 4155 269
Size: 792 1495
Fragments arranged by size:
1495 792 778 701 630 376 244 190
119 56
BsmBI CGTCTCn'nnnn_
Cuts at: 206 206
Size: 5381
Bsp1286I G_dGCh'C
Cuts at: 269 325 447 460 569 1199 1900 2019 2395
Size: 56 122 13 109 630 701 119 376
Cuts at: 2395 3006 3099 3173 3363 3425 4155 269
Size: 611 93 74 190 62 730 1495
Fragments arranged by size:
1495 730 701 630 611 376 190 122
119 109 93 74 62 56 13
BspEI T'CCGG_A
Cuts at: 4601 4601
Size: 5381
BspHI T'CATG_A
Cuts at: 1040 2088 4557 1040
Size: 1048 2469 1864
Fragments arranged by size:
2469 1864 1048
BspMI ACCTGCnnnn'nnnn_
Cuts at: 804 2329 2431 2645 2947 3328 804
Size: 1525 102 214 302 381 2857
Fragments arranged by size:
2857 1525 381 302 214 102
BsrBI CCG'CTC
Cuts at: 50 3673 3727 5211 5376 50
Size: 3623 54 1484 165 55
Fragments arranged by size:
3623 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1305 1430 3293 1305
Size: 125 1863 3393
Fragments arranged by size:
3393 1863 125
BsrFI r'CCGG_y
Cuts at: 211 634 3379 3560 3769 211
Size: 423 2745 181 209 1823
Fragments arranged by size:
2745 1823 423 209 181
BssSI C'ACGA_G
Cuts at: 426 3652 4010 426
Size: 3226 358 1797
Fragments arranged by size:
3226 1797 358
BstAPI GCAn_nnn'nTGC
Cuts at: 168 2758 168
Size: 2590 2791
BstBI TT'CG_AA
Cuts at: 110 3742 110
Size: 3632 1749
BstYI r'GATC_y
Cuts at: 1 1583 1845 2406 2873 2896 3231 3477 4489
Size: 1582 262 561 467 23 335 246 1012
Cuts at: 4489 4575 4587 4933 1
Size: 86 12 346 449
Fragments arranged by size:
1582 1012 561 467 449 346 335 262
246 86 23 12
BtgI C'CryG_G
Cuts at: 2168 2168
Size: 5381
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3331 3512 3675 3331
Size: 181 163 5037
Fragments arranged by size:
5037 181 163
BtsI GCAGTG_nn'
Cuts at: 258 1336 2624 258
Size: 1078 1288 3015
Fragments arranged by size:
3015 1288 1078
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1337 1372 1337
Size: 35 5346
DraI TTT'AAA
Cuts at: 2380 2380
Size: 5381
DrdI GACnn_nn'nnGTC
Cuts at: 3087 3945 3087
Size: 858 4523
EaeI y'GGCC_r
Cuts at: 636 839 1211 2051 2966 3140 3531 3558 4903
Size: 203 372 840 915 174 391 27 1345
Cuts at: 4903 636
Size: 1114
Fragments arranged by size:
1345 1114 915 840 391 372 203 174
27
EagI C'GGCC_G
Cuts at: 2966 2966
Size: 5381
EarI CTCTTCn'nnn_
Cuts at: 193 354 360 381 390 487 3404 3614 193
Size: 161 6 21 9 97 2917 210 1960
Fragments arranged by size:
2917 1960 210 161 97 21 9 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3589 3899 4045 5191 3589
Size: 310 146 1146 3779
Fragments arranged by size:
3779 1146 310 146
EcoICRI GAG'CTC
Cuts at: 567 1197 2393 567
Size: 630 1196 3555
Fragments arranged by size:
3555 1196 630
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 654 701 705 985 1836 3206 3638 4385
Size: 480 47 4 280 851 1370 432 747
Cuts at: 4385 4653 174
Size: 268 902
Fragments arranged by size:
1370 902 851 747 480 432 280 268
47 4
EcoNI CCTnn'n_nnAGG
Cuts at: 241 241
Size: 5381
EcoO109I rG'GnC_Cy
Cuts at: 274 918 1783 2520 274
Size: 644 865 737 3135
Fragments arranged by size:
3135 865 737 644
EcoRI G'AATT_C
Cuts at: 685 1073 2385 685
Size: 388 1312 3681
Fragments arranged by size:
3681 1312 388
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1472 3400 1472
Size: 1928 3453
FspI TGC'GCA
Cuts at: 3162 3162
Size: 5381
HaeII r_GCGC'y
Cuts at: 2815 3063 4085 5064 2815
Size: 248 1022 979 3132
Fragments arranged by size:
3132 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 652 684 1560 1592 2027 2059 3223 3253 3255
Size: 32 876 32 435 32 1164 30 2
Cuts at: 3255 3285 652
Size: 30 2748
Fragments arranged by size:
2748 1164 876 435 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1745 1858 2420 1745
Size: 113 562 4706
Fragments arranged by size:
4706 562 113
HindIII A'AGCT_T
Cuts at: 1045 1045
Size: 5381
KasI G'GCGC_C
Cuts at: 2811 3059 2811
Size: 248 5133
KpnI G_GTAC'C
Cuts at: 2401 2401
Size: 5381
MfeI C'AATT_G
Cuts at: 1886 1886
Size: 5381
MluI A'CGCG_T
Cuts at: 4814 4814
Size: 5381
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 851 1479 1920 2025 4052 4236 851
Size: 628 441 105 2027 184 1996
Fragments arranged by size:
2027 1996 628 441 184 105
MscI TGG'CCA
Cuts at: 1213 2053 3142 1213
Size: 840 1089 3452
Fragments arranged by size:
3452 1089 840
MslI CAynn'nnrTG
Cuts at: 1754 3370 3497 1754
Size: 1616 127 3638
Fragments arranged by size:
3638 1616 127
MspA1I CmG'CkG
Cuts at: 179 399 1189 2492 2806 3166 4179 4424 179
Size: 220 790 1303 314 360 1013 245 1136
Fragments arranged by size:
1303 1136 1013 790 360 314 245 220
NaeI GCC'GGC
Cuts at: 3562 3562
Size: 5381
NarI GG'CG_CC
Cuts at: 2812 3060 2812
Size: 248 5133
NcoI C'CATG_G
Cuts at: 2168 2168
Size: 5381
NgoMIV G'CCGG_C
Cuts at: 3560 3560
Size: 5381
NsiI A_TGCA'T
Cuts at: 1105 1260 1380 1105
Size: 155 120 5106
Fragments arranged by size:
5106 155 120
NspI r_CATG'y
Cuts at: 1287 1314 2013 2060 2434 2934 3841 1287
Size: 27 699 47 374 500 907 2827
Fragments arranged by size:
2827 907 699 500 374 47 27
PciI A'CATG_T
Cuts at: 2009 2056 3837 2009
Size: 47 1781 3553
Fragments arranged by size:
3553 1781 47
PflMI CCAn_nnn'nTGG
Cuts at: 1721 1721
Size: 5381
PfoI T'CCnGG_A
Cuts at: 531 531
Size: 5381
PmeI GTTT'AAAC
Cuts at: 2380 2380
Size: 5381
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4520 4552 4520
Size: 32 5349
PpuMI rG'GwC_Cy
Cuts at: 274 918 274
Size: 644 4737
PsiI TTA'TAA
Cuts at: 1327 1327
Size: 5381
PstI C_TGCA'G
Cuts at: 437 990 1619 2428 3113 437
Size: 553 629 809 685 2705
Fragments arranged by size:
2705 809 685 629 553
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5381
PvuII CAG'CTG
Cuts at: 1189 2806 3166 1189
Size: 1617 360 3404
Fragments arranged by size:
3404 1617 360
RsrII CG'GwC_CG
Cuts at: 3576 3576
Size: 5381
SacI G_AGCT'C
Cuts at: 569 1199 2395 569
Size: 630 1196 3555
Fragments arranged by size:
3555 1196 630
SalI G'TCGA_C
Cuts at: 2418 2418
Size: 5381
SanDI GG'GwC_CC
Cuts at: 274 274
Size: 5381
SapI GCTCTTCn'nnn_
Cuts at: 3404 3614 3404
Size: 210 5171
SbfI CC_TGCA'GG
Cuts at: 2428 2428
Size: 5381
ScaI AGT'ACT
Cuts at: 1775 4898 1775
Size: 3123 2258
SfcI C'TryA_G
Cuts at: 33 433 986 1493 1514 1550 1615 2424 3109
Size: 400 553 507 21 36 65 809 685
Cuts at: 3109 4102 4293 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 809 685 553 507 400 191
65 36 21
SfoI GGC'GCC
Cuts at: 2813 3061 2813
Size: 248 5133
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5381
SgrAI Cr'CCGG_yG
Cuts at: 211 211
Size: 5381
SmaI CCC'GGG
Cuts at: 2403 2403
Size: 5381
SmlI C'TyrA_G
Cuts at: 1094 2536 3451 3943 4205 1094
Size: 1442 915 492 262 2270
Fragments arranged by size:
2270 1442 915 492 262
SnaBI TAC'GTA
Cuts at: 2373 2373
Size: 5381
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5381
SphI G_CATG'C
Cuts at: 1314 2434 2934 1314
Size: 1120 500 3761
Fragments arranged by size:
3761 1120 500
SspI AAT'ATT
Cuts at: 1981 2328 1981
Size: 347 5034
StyI C'CwwG_G
Cuts at: 2168 2217 2515 2869 2168
Size: 49 298 354 4680
Fragments arranged by size:
4680 354 298 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2964 3097 3556 3765 2964
Size: 133 459 209 4580
Fragments arranged by size:
4580 459 209 133
TatI w'GTAC_w
Cuts at: 1773 4896 1773
Size: 3123 2258
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 399 431 1998 2025 2030 2057 2325 2357 4898
Size: 32 1567 27 5 27 268 32 2541
Cuts at: 4898 4930 399
Size: 32 850
Fragments arranged by size:
2541 1567 850 268 32 32 32 27
27 5
Tth111I GACn'n_nGTC
Cuts at: 922 1757 3178 922
Size: 835 1421 3125
Fragments arranged by size:
3125 1421 835
XbaI T'CTAG_A
Cuts at: 1416 2412 1416
Size: 996 4385
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2876 2876
Size: 5381
XmaI C'CCGG_G
Cuts at: 2401 2401
Size: 5381
XmnI GAAnn'nnTTC
Cuts at: 187 187
Size: 5381
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PflMI PfoI PmeI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BmgBI BmtI BsiWI BsmI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII EcoRV
FseI FspAI HpaI NdeI NheI NotI NruI PacI
PasI PmlI PshAI PspOMI PspXI PsrI SacII SexAI
SfiI SrfI StuI SwaI XhoI ZraI