Restriction map for
pF1KSDA1498
(Circular) (Six-base) MAPSORT of: pF1KSDA1498.seq Check: 7486 from: 1 to: 12266
pF1KSDA1498 12266 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 571 957 2010 6795 6966 8025 9024 9090 571
Size: 386 1053 4785 171 1059 999 66 3747
Fragments arranged by size:
4785 3747 1059 1053 999 386 171 66
AatII G_ACGT'C
Cuts at: 1069 3197 5520 1069
Size: 2128 2323 7815
Fragments arranged by size:
7815 2323 2128
AccI GT'mk_AC
Cuts at: 6476 9304 6476
Size: 2828 9438
Acc65I G'GTAC_C
Cuts at: 9282 9282
Size: 12266
AclI AA'CG_TT
Cuts at: 3498 12106 3498
Size: 8608 3658
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1282 3230 4228 4337 4411 5479 5531 6397 6817
Size: 1948 998 109 74 1068 52 866 420
Cuts at: 6817 8698 8704 10091 10523 11270 11538 1282
Size: 1881 6 1387 432 747 268 2010
Fragments arranged by size:
2010 1948 1881 1387 1068 998 866 747
432 420 268 109 74 52 6
AfeI AGC'GCT
Cuts at: 447 447
Size: 12266
AflII C'TTAA_G
Cuts at: 8827 8827
Size: 12266
AflIII A'CryG_T
Cuts at: 7256 9618 10722 11699 7256
Size: 2362 1104 977 7823
Fragments arranged by size:
7823 2362 1104 977
AgeI A'CCGG_T
Cuts at: 10654 10654
Size: 12266
AhdI GACnn_n'nnGTC
Cuts at: 527 5225 7857 527
Size: 4698 2632 4936
Fragments arranged by size:
4936 4698 2632
AleI CACnn'nnGTG
Cuts at: 1138 3202 3646 5737 6949 7897 8410 1138
Size: 2064 444 2091 1212 948 513 4994
Fragments arranged by size:
4994 2091 2064 1212 948 513 444
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1781 3413 4117 5101 8819 1781
Size: 1632 704 984 3718 5228
Fragments arranged by size:
5228 3718 1632 984 704
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 728 760 728
Size: 32 12234
AlwNI CAG_nnn'CTG
Cuts at: 182 306 882 972 1230 1260 4725 5367 5400
Size: 124 576 90 258 30 3465 642 33
Cuts at: 5400 5640 5961 6184 6924 7636 8250 8673 8676
Size: 240 321 223 740 712 614 423 3
Cuts at: 8676 8871 9571 11138 182
Size: 195 700 1567 1310
Fragments arranged by size:
3465 1567 1310 740 712 700 642 614
576 423 321 258 240 223 195 124
90 33 30 3
ApaI G_GGCC'C
Cuts at: 367 3442 3858 4519 8740 8883 367
Size: 3075 416 661 4221 143 3750
Fragments arranged by size:
4221 3750 3075 661 416 143
ApaLI G'TGCA_C
Cuts at: 3802 4831 7798 8659 11036 3802
Size: 1029 2967 861 2377 5032
Fragments arranged by size:
5032 2967 2377 1029 861
ApoI r'AATT_y
Cuts at: 2730 6755 9270 11619 11875 2730
Size: 4025 2515 2349 256 3121
Fragments arranged by size:
4025 3121 2515 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 12266
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 12266
AvaI C'yCGr_G
Cuts at: 759 1033 1798 2639 3236 4435 4765 6871 8441
Size: 274 765 841 597 1199 330 2106 1570
Cuts at: 8441 9286 759
Size: 845 3739
Fragments arranged by size:
3739 2106 1570 1199 845 841 765 597
330 274
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4168 4201 5685 5718 4168
Size: 33 1484 33 10716
Fragments arranged by size:
10716 1484 33 33
BamHI G'GATC_C
Cuts at: 9291 9291
Size: 12266
BanI G'GyrC_C
Cuts at: 273 583 820 2992 4148 4837 5313 6807 7657
Size: 310 237 2172 1156 689 476 1494 850
Cuts at: 7657 7962 8389 8404 8490 9159 9282 9696 9944
Size: 305 427 15 86 669 123 414 248
Cuts at: 9944 9979 273
Size: 35 2560
Fragments arranged by size:
2560 2172 1494 1156 850 689 669 476
427 414 310 305 248 237 123 86
35 15
BanII G_rGCy'C
Cuts at: 322 367 676 719 741 850 3288 3442 3791
Size: 45 309 43 22 109 2438 154 349
Cuts at: 3791 3844 3858 4136 4145 4519 4788 6434 8356
Size: 53 14 278 9 374 269 1646 1922
Cuts at: 8356 8740 8883 9280 10310 322
Size: 384 143 397 1030 2278
Fragments arranged by size:
2438 2278 1922 1646 1030 397 384 374
349 309 278 269 154 143 109 53
45 43 22 14 9
BbeI G_GCGC'C
Cuts at: 2996 4841 9700 9948 2996
Size: 1845 4859 248 5314
Fragments arranged by size:
5314 4859 1845 248
BbsI GAAGACnn'nnnn_
Cuts at: 1263 1915 6385 1263
Size: 652 4470 7144
Fragments arranged by size:
7144 4470 652
BbvCI CC'TCA_GC
Cuts at: 258 2202 2380 5443 6011 6663 258
Size: 1944 178 3063 568 652 5861
Fragments arranged by size:
5861 3063 1944 652 568 178
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 251 285 5596 5630 251
Size: 34 5311 34 6887
Fragments arranged by size:
6887 5311 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2316 4196 10164 10925 12266 2316
Size: 1880 5968 761 1341 2316
Fragments arranged by size:
5968 2316 1880 1341 761
BclI T'GATC_A
Cuts at: 7365 9786 7365
Size: 2421 9845
BglI GCCn_nnn'nGGC
Cuts at: 1803 2635 7494 7609 8509 1803
Size: 832 4859 115 900 5560
Fragments arranged by size:
5560 4859 900 832 115
BglII A'GATC_T
Cuts at: 4242 5886 7115 9781 11818 4242
Size: 1644 1229 2666 2037 4690
Fragments arranged by size:
4690 2666 2037 1644 1229
BlpI GC'TnA_GC
Cuts at: 449 2954 4595 4634 6608 6851 9378 449
Size: 2505 1641 39 1974 243 2527 3337
Fragments arranged by size:
3337 2527 2505 1974 1641 243 39
Bme1580I G_kGCm'C
Cuts at: 367 588 823 1109 3442 3806 3858 4076 4519
Size: 221 235 286 2333 364 52 218 443
Cuts at: 4519 4835 5103 7802 7967 8394 8407 8663 8740
Size: 316 268 2699 165 427 13 256 77
Cuts at: 8740 8883 9164 9891 9984 11040 367
Size: 143 281 727 93 1056 1593
Fragments arranged by size:
2699 2333 1593 1056 727 443 427 364
316 286 281 268 256 235 221 218
165 143 93 77 52 13
BmgBI CAC'GTC
Cuts at: 497 1136 3594 3605 497
Size: 639 2458 11 9158
Fragments arranged by size:
9158 2458 639 11
BmrI ACTGGGnnnn_n'
Cuts at: 273 281 536 2460 3720 3854 6903 7646 7930
Size: 8 255 1924 1260 134 3049 743 284
Cuts at: 7930 8172 8280 8829 9478 9559 9894 12038 273
Size: 242 108 549 649 81 335 2144 501
Fragments arranged by size:
3049 2144 1924 1260 743 649 549 501
335 284 255 242 134 108 81 8
BmtI G_CTAG'C
Cuts at: 2840 6232 2840
Size: 3392 8874
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2303 3863 4362 8011 10633 2303
Size: 1560 499 3649 2622 3936
Fragments arranged by size:
3936 3649 2622 1560 499
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 413 663 736 1627 2168 2210 2368 2485 3805
Size: 250 73 891 541 42 158 117 1320
Cuts at: 3805 3810 4201 4237 4732 5051 6133 6433 6946
Size: 5 391 36 495 319 1082 300 513
Cuts at: 6946 8407 9112 9119 413
Size: 1461 705 7 3560
Fragments arranged by size:
3560 1461 1320 1082 891 705 541 513
495 391 319 300 250 158 117 73
42 36 7 5
Bpu10I CC'TnA_GC
Cuts at: 258 2202 2380 3838 4090 5089 5438 5443 5783
Size: 1944 178 1458 252 999 349 5 340
Cuts at: 5783 5909 6011 6663 258
Size: 126 102 652 5861
Fragments arranged by size:
5861 1944 1458 999 652 349 340 252
178 126 102 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 690 3460 5045 5376 6079 8906 9442 10321 10813
Size: 2770 1585 331 703 2827 536 879 492
Cuts at: 10813 11111 690
Size: 298 1845
Fragments arranged by size:
2827 2770 1845 1585 879 703 536 492
331 298
BsaI GGTCTCn'nnnn_
Cuts at: 479 783 2760 8559 11970 479
Size: 304 1977 5799 3411 775
Fragments arranged by size:
5799 3411 1977 775 304
BsaAI yAC'GTr
Cuts at: 4167 6627 9258 9619 4167
Size: 2460 2631 361 6814
Fragments arranged by size:
6814 2631 2460 361
BsaBI GATnn'nnATC
Cuts at: 6 3160 9780 11817 6
Size: 3154 6620 2037 455
Fragments arranged by size:
6620 3154 2037 455
BsaHI Gr'CG_yC
Cuts at: 1066 2409 2993 3194 4645 4838 5517 7738 8215
Size: 1343 584 201 1451 193 679 2221 477
Cuts at: 8215 9115 9697 9945 10647 12143 1066
Size: 900 582 248 702 1496 1189
Fragments arranged by size:
2221 1496 1451 1343 1189 900 702 679
584 582 477 248 201 193
BsaWI w'CCGG_w
Cuts at: 1200 4560 5283 8526 9455 9976 10654 10928 11075
Size: 3360 723 3243 929 521 678 274 147
Cuts at: 11075 11486 1200
Size: 411 1980
Fragments arranged by size:
3360 3243 1980 929 723 678 521 411
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 395 425 728 758 1734 1764 2461 2491 2494
Size: 30 303 30 976 30 697 30 3
Cuts at: 2494 2524 2635 2665 3826 3856 4174 4204 4568
Size: 30 111 30 1161 30 318 30 364
Cuts at: 4568 4598 5400 5430 9228 9258 395
Size: 30 802 30 3798 30 3403
Fragments arranged by size:
3798 3403 1161 976 802 697 364 318
303 111 30 30 30 30 30 30
30 30 30 30 30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 217 1195 1423 1426 1429 1432 1441 1830 2482
Size: 978 228 3 3 3 9 389 652
Cuts at: 2482 2520 2578 2905 3948 5661 6028 6130 6238
Size: 38 58 327 1043 1713 367 102 108
Cuts at: 6238 6436 6704 7087 7212 8547 217
Size: 198 268 383 125 1335 3936
Fragments arranged by size:
3936 1713 1335 1043 978 652 389 383
367 327 268 228 198 125 108 102
58 38 9 3 3 3
BseYI C'CCAG_C
Cuts at: 415 509 600 797 1039 1191 1812 1945 3370
Size: 94 91 197 242 152 621 133 1425
Cuts at: 3370 3375 4362 4459 4870 6150 7148 7397 7412
Size: 5 987 97 411 1280 998 249 15
Cuts at: 7412 7520 7538 7560 7701 7805 7833 8184 8357
Size: 108 18 22 141 104 28 351 173
Cuts at: 8357 8495 8651 8882 8978 9150 9691 10449 11026
Size: 138 156 231 96 172 541 758 577
Cuts at: 11026 11522 415
Size: 496 1159
Fragments arranged by size:
1425 1280 1159 998 987 758 621 577
541 496 411 351 249 242 231 197
173 172 156 152 141 138 133 108
104 97 96 94 91 28 22 18
15 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 599 1777 1922 1964 2038 2044 3409 3754 4438
Size: 1178 145 42 74 6 1365 345 684
Cuts at: 4438 4862 5473 7349 7573 7619 7960 8003 8344
Size: 424 611 1876 224 46 341 43 341
Cuts at: 8344 8714 8911 9058 599
Size: 370 197 147 3807
Fragments arranged by size:
3807 1876 1365 1178 684 611 424 370
345 341 341 224 197 147 145 74
46 43 42 6
BsiEI CG_ry'CG
Cuts at: 104 1661 3025 4534 4862 5323 6707 7391 7913
Size: 1557 1364 1509 328 461 1384 684 522
Cuts at: 7913 8612 9026 9808 9854 11062 104
Size: 699 414 782 46 1208 1308
Fragments arranged by size:
1557 1509 1384 1364 1308 1208 782 699
684 522 461 414 328 46
BsiHKAI G_wGCw'C
Cuts at: 3237 3791 3806 3844 4835 5378 6434 7802 8663
Size: 554 15 38 991 543 1056 1368 861
Cuts at: 8663 9280 10058 10248 11040 3237
Size: 617 778 190 792 4463
Fragments arranged by size:
4463 1368 1056 991 861 792 778 617
554 543 190 38 15
BsiWI C'GTAC_G
Cuts at: 7105 7105
Size: 12266
BsmI GAATG_Cn'
Cuts at: 1679 2298 4929 7140 1679
Size: 619 2631 2211 6805
Fragments arranged by size:
6805 2631 2211 619
BsmBI CGTCTCn'nnnn_
Cuts at: 1504 4636 1504
Size: 3132 9134
Bsp1286I G_dGCh'C
Cuts at: 322 367 588 676 719 741 823 850 1109
Size: 45 221 88 43 22 82 27 259
Cuts at: 1109 3237 3288 3442 3791 3806 3844 3858 4076
Size: 2128 51 154 349 15 38 14 218
Cuts at: 4076 4136 4145 4519 4788 4835 5103 5378 6434
Size: 60 9 374 269 47 268 275 1056
Cuts at: 6434 7802 7967 8356 8394 8407 8663 8740 8883
Size: 1368 165 389 38 13 256 77 143
Cuts at: 8883 9164 9280 9891 9984 10058 10248 10310 11040
Size: 281 116 611 93 74 190 62 730
Cuts at: 11040 322
Size: 1548
Fragments arranged by size:
2128 1548 1368 1056 730 611 389 374
349 281 275 269 268 259 256 221
218 190 165 154 143 116 93 88
82 77 74 62 60 51 47 45
43 38 38 27 22 15 14 13
9
BspEI T'CCGG_A
Cuts at: 1200 4560 11486 1200
Size: 3360 6926 1980
Fragments arranged by size:
6926 3360 1980
BspHI T'CATG_A
Cuts at: 11442 11442
Size: 12266
BspMI ACCTGCnnnn'nnnn_
Cuts at: 504 571 957 2010 2380 4391 6795 6966 8025
Size: 67 386 1053 370 2011 2404 171 1059
Cuts at: 8025 9024 9090 9316 9530 9832 10213 504
Size: 999 66 226 214 302 381 2557
Fragments arranged by size:
2557 2404 2011 1059 1053 999 386 381
370 302 226 214 171 67 66
BsrBI CCG'CTC
Cuts at: 50 411 786 4070 5049 6513 6639 7022 7888
Size: 361 375 3284 979 1464 126 383 866
Cuts at: 7888 9126 10558 10612 12096 12261 50
Size: 1238 1432 54 1484 165 55
Fragments arranged by size:
3284 1484 1464 1432 1238 979 866 383
375 361 165 126 55 54
BsrDI GCAATG_nn'
Cuts at: 2369 3185 3395 6056 10178 2369
Size: 816 210 2661 4122 4457
Fragments arranged by size:
4457 4122 2661 816 210
BsrFI r'CCGG_y
Cuts at: 210 868 1816 4647 4861 7386 8820 10264 10445
Size: 658 948 2831 214 2525 1434 1444 181
Cuts at: 10445 10654 210
Size: 209 1822
Fragments arranged by size:
2831 2525 1822 1444 1434 948 658 214
209 181
BsrGI T'GTAC_A
Cuts at: 5333 7154 5333
Size: 1821 10445
BssHII G'CGCG_C
Cuts at: 8789 8789
Size: 12266
BssSI C'ACGA_G
Cuts at: 2560 3644 4291 7067 7158 10537 10895 2560
Size: 1084 647 2776 91 3379 358 3931
Fragments arranged by size:
3931 3379 2776 1084 647 358 91
BstAPI GCAn_nnn'nTGC
Cuts at: 165 564 573 969 972 1539 1653 6807 8250
Size: 399 9 396 3 567 114 5154 1443
Cuts at: 8250 8673 9036 9643 165
Size: 423 363 607 2788
Fragments arranged by size:
5154 2788 1443 607 567 423 399 396
363 114 9 3
BstBI TT'CG_AA
Cuts at: 110 10627 110
Size: 10517 1749
BstEII G'GTnAC_C
Cuts at: 1591 9105 1591
Size: 7514 4752
BstXI CCAn_nnnn'nTGG
Cuts at: 139 651 1554 7314 139
Size: 512 903 5760 5091
Fragments arranged by size:
5760 5091 903 512
BstYI r'GATC_y
Cuts at: 1 3821 4242 5351 5886 6978 7115 8162 8900
Size: 3820 421 1109 535 1092 137 1047 738
Cuts at: 8900 9291 9758 9781 10116 10362 11374 11460 11472
Size: 391 467 23 335 246 1012 86 12
Cuts at: 11472 11818 1
Size: 346 449
Fragments arranged by size:
3820 1109 1092 1047 1012 738 535 467
449 421 391 346 335 246 137 86
23 12
BtgI C'CryG_G
Cuts at: 132 857 905 1742 2101 2633 3797 4324 4448
Size: 725 48 837 359 532 1164 527 124
Cuts at: 4448 4493 5112 5317 5411 5927 5981 7391 7816
Size: 45 619 205 94 516 54 1410 425
Cuts at: 7816 7838 7862 7913 8411 8642 8945 9110 132
Size: 22 24 51 498 231 303 165 3288
Fragments arranged by size:
3288 1410 1164 837 725 619 532 527
516 498 425 359 303 231 205 165
124 94 54 51 48 45 24 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1552 4524 7674 10216 10397 10560 1552
Size: 2972 3150 2542 181 163 3258
Fragments arranged by size:
3258 3150 2972 2542 181 163
BtsI GCAGTG_nn'
Cuts at: 403 1231 1783 1826 2482 3197 3237 4654 5628
Size: 828 552 43 656 715 40 1417 974
Cuts at: 5628 6344 6356 7602 9059 9509 403
Size: 716 12 1246 1457 450 3160
Fragments arranged by size:
3160 1457 1417 1246 974 828 716 715
656 552 450 43 40 12
ClaI AT'CG_AT
Cuts at: 5606 5606
Size: 12266
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2475 2510 3400 3435 2475
Size: 35 890 35 11306
Fragments arranged by size:
11306 890 35 35
DraI TTT'AAA
Cuts at: 6275 9265 6275
Size: 2990 9276
DraIII CAC_nnn'GTG
Cuts at: 696 3912 696
Size: 3216 9050
DrdI GACnn_nn'nnGTC
Cuts at: 9114 9972 10830 9114
Size: 858 858 10550
Fragments arranged by size:
10550 858 858
EaeI y'GGCC_r
Cuts at: 884 2483 3022 3323 4425 4531 4859 5145 5320
Size: 1599 539 301 1102 106 328 286 175
Cuts at: 5320 7388 7910 8414 8873 8948 9023 9851 10025
Size: 2068 522 504 459 75 75 828 174
Cuts at: 10025 10416 10443 11788 884
Size: 391 27 1345 1362
Fragments arranged by size:
2068 1599 1362 1345 1102 828 539 522
504 459 391 328 301 286 175 174
106 75 75 27
EagI C'GGCC_G
Cuts at: 3022 4531 4859 5320 7388 7910 9023 9851 3022
Size: 1509 328 461 2068 522 1113 828 5437
Fragments arranged by size:
5437 2068 1509 1113 828 522 461 328
EarI CTCTTCn'nnn_
Cuts at: 1400 1418 2124 2594 2867 2882 3860 4060 4164
Size: 18 706 470 273 15 978 200 104
Cuts at: 4164 5852 7029 7075 10289 10499 1400
Size: 1688 1177 46 3214 210 3167
Fragments arranged by size:
3214 3167 1688 1177 978 706 470 273
210 200 104 46 18 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1639 2634 4749 5682 5743 6442 10474 10784 10930
Size: 995 2115 933 61 699 4032 310 146
Cuts at: 10930 12076 1639
Size: 1146 1829
Fragments arranged by size:
4032 2115 1829 1146 995 933 699 310
146 61
EcoICRI GAG'CTC
Cuts at: 3789 3842 6432 9278 3789
Size: 53 2590 2846 6777
Fragments arranged by size:
6777 2846 2590 53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 413 663 736 1282 1627 2168 2210 2368 2485
Size: 250 73 546 345 541 42 158 117
Cuts at: 2485 3230 3805 3810 4201 4228 4237 4337 4411
Size: 745 575 5 391 27 9 100 74
Cuts at: 4411 4732 5051 5479 5531 6133 6397 6433 6817
Size: 321 319 428 52 602 264 36 384
Cuts at: 6817 6946 8407 8698 8704 9112 9119 10091 10523
Size: 129 1461 291 6 408 7 972 432
Cuts at: 10523 11270 11538 413
Size: 747 268 1141
Fragments arranged by size:
1461 1141 972 747 745 602 575 546
541 432 428 408 391 384 345 321
319 291 268 264 250 158 129 117
100 74 73 52 42 36 27 9
7 6 5
EcoNI CCTnn'n_nnAGG
Cuts at: 3223 5722 3223
Size: 2499 9767
EcoO109I rG'GnC_Cy
Cuts at: 179 364 838 1747 2161 2430 3438 3439 3627
Size: 185 474 909 414 269 1008 1 188
Cuts at: 3627 3830 3854 3855 4515 5135 5364 6090 7557
Size: 203 24 1 660 620 229 726 1467
Cuts at: 7557 7698 7757 8736 8879 9210 9405 179
Size: 141 59 979 143 331 195 3040
Fragments arranged by size:
3040 1467 1008 979 909 726 660 620
474 414 331 269 229 203 195 188
185 143 141 59 24 1 1
EcoRI G'AATT_C
Cuts at: 9270 9270
Size: 12266
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1287 1393 2742 4261 10285 1287
Size: 106 1349 1519 6024 3268
Fragments arranged by size:
6024 3268 1519 1349 106
FseI GG_CCGG'CC
Cuts at: 872 872
Size: 12266
FspI TGC'GCA
Cuts at: 10047 10047
Size: 12266
HaeII r_GCGC'y
Cuts at: 449 2996 4829 4841 5022 5055 6901 7734 9007
Size: 2547 1833 12 181 33 1846 833 1273
Cuts at: 9007 9143 9700 9948 10970 11949 449
Size: 136 557 248 1022 979 766
Fragments arranged by size:
2547 1846 1833 1273 1022 979 833 766
557 248 181 136 33 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 457 489 513 545 1242 1274 1704 1736 3503
Size: 32 24 32 697 32 430 32 1767
Cuts at: 3503 3535 4174 4206 4291 4323 4800 4832 5211
Size: 32 639 32 85 32 477 32 379
Cuts at: 5211 5243 5400 5432 6157 6189 7843 7875 8127
Size: 32 157 32 725 32 1654 32 252
Cuts at: 8127 8159 10108 10138 10140 10170 457
Size: 32 1949 30 2 30 2553
Fragments arranged by size:
2553 1949 1767 1654 725 697 639 477
430 379 252 157 85 32 32 32
32 32 32 32 32 32 32 32
32 32 30 30 24 2
HincII GTy'rAC
Cuts at: 4083 5043 5219 6999 9305 4083
Size: 960 176 1780 2306 7044
Fragments arranged by size:
7044 2306 1780 960 176
HindIII A'AGCT_T
Cuts at: 5477 5477
Size: 12266
HpaI GTT'AAC
Cuts at: 6999 6999
Size: 12266
KasI G'GCGC_C
Cuts at: 2992 4837 9696 9944 2992
Size: 1845 4859 248 5314
Fragments arranged by size:
5314 4859 1845 248
KpnI G_GTAC'C
Cuts at: 9286 9286
Size: 12266
MfeI C'AATT_G
Cuts at: 2882 6257 2882
Size: 3375 8891
MluI A'CGCG_T
Cuts at: 7256 11699 7256
Size: 4443 7823
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5453 5534 9197 10937 11121 5453
Size: 81 3663 1740 184 6598
Fragments arranged by size:
6598 3663 1740 184 81
MscI TGG'CCA
Cuts at: 886 2485 3325 4427 8416 8950 10027 886
Size: 1599 840 1102 3989 534 1077 3125
Fragments arranged by size:
3989 3125 1599 1102 1077 840 534
MslI CAynn'nnrTG
Cuts at: 1138 1153 1552 3202 3646 4099 5737 6164 6949
Size: 15 399 1650 444 453 1638 427 785
Cuts at: 6949 7312 7897 8233 8410 10255 10382 1138
Size: 363 585 336 177 1845 127 3022
Fragments arranged by size:
3022 1845 1650 1638 785 585 453 444
427 399 363 336 177 127 15
MspA1I CmG'CkG
Cuts at: 165 306 859 951 987 1051 1794 1986 3768
Size: 141 553 92 36 64 743 192 1782
Cuts at: 3768 4326 4599 4714 4725 4731 5319 5400 6667
Size: 558 273 115 11 6 588 81 1267
Cuts at: 6667 6850 7152 7299 7455 7474 7504 7705 8460
Size: 183 302 147 156 19 30 201 755
Cuts at: 8460 8548 8673 9377 9691 10051 11064 11309 165
Size: 88 125 704 314 360 1013 245 1122
Fragments arranged by size:
1782 1267 1122 1013 755 743 704 588
558 553 360 314 302 273 245 201
192 183 156 147 141 125 115 92
88 81 64 36 30 19 11 6
NaeI GCC'GGC
Cuts at: 212 870 4863 7388 8822 10447 212
Size: 658 3993 2525 1434 1625 2031
Fragments arranged by size:
3993 2525 2031 1625 1434 658
NarI GG'CG_CC
Cuts at: 2993 4838 9697 9945 2993
Size: 1845 4859 248 5314
Fragments arranged by size:
5314 4859 1845 248
NcoI C'CATG_G
Cuts at: 132 3797 5112 5927 5981 7862 132
Size: 3665 1315 815 54 1881 4536
Fragments arranged by size:
4536 3665 1881 1315 815 54
NgoMIV G'CCGG_C
Cuts at: 210 868 4861 7386 8820 10445 210
Size: 658 3993 2525 1434 1625 2031
Fragments arranged by size:
3993 2525 2031 1625 1434 658
NheI G'CTAG_C
Cuts at: 2836 6228 2836
Size: 3392 8874
NotI GC'GGCC_GC
Cuts at: 5320 5320
Size: 12266
NspI r_CATG'y
Cuts at: 3985 6195 7650 8667 9319 9819 10726 3985
Size: 2210 1455 1017 652 500 907 5525
Fragments arranged by size:
5525 2210 1455 1017 907 652 500
PasI CC'CwG_GG
Cuts at: 595 732 999 1618 2245 3817 4129 4842 595
Size: 137 267 619 627 1572 312 713 8019
Fragments arranged by size:
8019 1572 713 627 619 312 267 137
PciI A'CATG_T
Cuts at: 10722 10722
Size: 12266
PflMI CCAn_nnn'nTGG
Cuts at: 834 3891 4897 5074 8386 8871 834
Size: 3057 1006 177 3312 485 4229
Fragments arranged by size:
4229 3312 3057 1006 485 177
PfoI T'CCnGG_A
Cuts at: 1093 2236 2746 2966 3507 3782 5954 1093
Size: 1143 510 220 541 275 2172 7405
Fragments arranged by size:
7405 2172 1143 541 510 275 220
PmeI GTTT'AAAC
Cuts at: 9265 9265
Size: 12266
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 728 760 11405 11437 728
Size: 32 10645 32 1557
Fragments arranged by size:
10645 1557 32 32
PpuMI rG'GwC_Cy
Cuts at: 838 2161 5135 7698 9210 838
Size: 1323 2974 2563 1512 3894
Fragments arranged by size:
3894 2974 2563 1512 1323
PshAI GACnn'nnGTC
Cuts at: 6502 6502
Size: 12266
PspOMI G'GGCC_C
Cuts at: 363 3438 3854 4515 8736 8879 363
Size: 3075 416 661 4221 143 3750
Fragments arranged by size:
4221 3750 3075 661 416 143
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5569 5601 5569
Size: 32 12234
PstI C_TGCA'G
Cuts at: 170 356 613 855 878 977 1833 1853 2426
Size: 186 257 242 23 99 856 20 573
Cuts at: 2426 3734 4112 4661 4730 5249 5432 6351 6389
Size: 1308 378 549 69 519 183 919 38
Cuts at: 6389 6672 6908 8255 8465 9054 9313 9998 170
Size: 283 236 1347 210 589 259 685 2438
Fragments arranged by size:
2438 1347 1308 919 856 685 589 573
549 519 378 283 259 257 242 236
210 186 183 99 69 38 23 20
PvuI CG_AT'CG
Cuts at: 104 8612 104
Size: 8508 3758
PvuII CAG'CTG
Cuts at: 165 306 951 1794 1986 4599 4731 5400 6667
Size: 141 645 843 192 2613 132 669 1267
Cuts at: 6667 6850 7152 7504 7705 8460 8548 8673 9691
Size: 183 302 352 201 755 88 125 1018
Cuts at: 9691 10051 165
Size: 360 2380
Fragments arranged by size:
2613 2380 1267 1018 843 755 669 645
360 352 302 201 192 183 141 132
125 88
RsrII CG'GwC_CG
Cuts at: 10461 10461
Size: 12266
SacI G_AGCT'C
Cuts at: 3791 3844 6434 9280 3791
Size: 53 2590 2846 6777
Fragments arranged by size:
6777 2846 2590 53
SacII CC_GC'GG
Cuts at: 860 4327 5320 860
Size: 3467 993 7806
Fragments arranged by size:
7806 3467 993
SalI G'TCGA_C
Cuts at: 9303 9303
Size: 12266
SanDI GG'GwC_CC
Cuts at: 838 5135 7698 9210 838
Size: 4297 2563 1512 3894
Fragments arranged by size:
4297 3894 2563 1512
SapI GCTCTTCn'nnn_
Cuts at: 2594 4060 4164 7029 10289 10499 2594
Size: 1466 104 2865 3260 210 4361
Fragments arranged by size:
4361 3260 2865 1466 210 104
SbfI CC_TGCA'GG
Cuts at: 6389 9313 6389
Size: 2924 9342
ScaI AGT'ACT
Cuts at: 11783 11783
Size: 12266
SexAI A'CCwGG_T
Cuts at: 2354 7853 2354
Size: 5499 6767
SfcI C'TryA_G
Cuts at: 33 166 352 609 623 851 874 973 1829
Size: 133 186 257 14 228 23 99 856
Cuts at: 1829 1849 2422 3730 4108 4657 4726 4934 5245
Size: 20 573 1308 378 549 69 208 311
Cuts at: 5245 5428 6347 6385 6459 6668 6904 7005 8251
Size: 183 919 38 74 209 236 101 1246
Cuts at: 8251 8461 9050 9309 9994 10987 11178 33
Size: 210 589 259 685 993 191 1121
Fragments arranged by size:
1308 1246 1121 993 919 856 685 589
573 549 378 311 259 257 236 228
210 209 208 191 186 183 133 101
99 74 69 38 23 20 14
SfoI GGC'GCC
Cuts at: 2994 4839 9698 9946 2994
Size: 1845 4859 248 5314
Fragments arranged by size:
5314 4859 1845 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 12266
SgrAI Cr'CCGG_yG
Cuts at: 4647 4647
Size: 12266
SmaI CCC'GGG
Cuts at: 761 1035 1800 2641 4767 8443 9288 761
Size: 274 765 841 2126 3676 845 3739
Fragments arranged by size:
3739 3676 2126 845 841 765 274
SmlI C'TyrA_G
Cuts at: 669 3475 5060 5355 6094 8827 8921 9421 10336
Size: 2806 1585 295 739 2733 94 500 915
Cuts at: 10336 10828 11090 669
Size: 492 262 1845
Fragments arranged by size:
2806 2733 1845 1585 915 739 500 492
295 262 94
SnaBI TAC'GTA
Cuts at: 9258 9258
Size: 12266
SpeI A'CTAG_T
Cuts at: 67 67
Size: 12266
SphI G_CATG'C
Cuts at: 7650 9319 9819 7650
Size: 1669 500 10097
Fragments arranged by size:
10097 1669 500
SspI AAT'ATT
Cuts at: 2667 6789 2667
Size: 4122 8144
StuI AGG'CCT
Cuts at: 562 1115 1575 2029 2725 4060 8385 562
Size: 553 460 454 696 1335 4325 4443
Fragments arranged by size:
4443 4325 1335 696 553 460 454
StyI C'CwwG_G
Cuts at: 132 1596 2945 3179 3797 4928 5112 5927 5981
Size: 1464 1349 234 618 1131 184 815 54
Cuts at: 5981 7862 8380 8513 9400 9754 132
Size: 1881 518 133 887 354 2644
Fragments arranged by size:
2644 1881 1464 1349 1131 887 815 618
518 354 234 184 133 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 736 1631 3240 3715 4166 6078 6540 6555 6707
Size: 895 1609 475 451 1912 462 15 152
Cuts at: 6707 7162 7819 8602 8865 9849 9982 10441 10650
Size: 455 657 783 263 984 133 459 209
Cuts at: 10650 736
Size: 2352
Fragments arranged by size:
2352 1912 1609 984 895 783 657 475
462 459 455 451 263 209 152 133
15
TatI w'GTAC_w
Cuts at: 5333 7154 11781 5333
Size: 1821 4627 5818
Fragments arranged by size:
5818 4627 1821
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 395 427 680 712 1734 1766 2461 2493 2494
Size: 32 253 32 1022 32 695 32 1
Cuts at: 2494 2526 2635 2667 4568 4600 4646 4678 5507
Size: 32 109 32 1901 32 46 32 829
Cuts at: 5507 5539 5661 5693 7490 7522 8430 8462 8627
Size: 32 122 32 1797 32 908 32 165
Cuts at: 8627 8659 11783 11815 395
Size: 32 3124 32 846
Fragments arranged by size:
3124 1901 1797 1022 908 846 829 695
253 165 122 109 46 32 32 32
32 32 32 32 32 32 32 32
32 32 32 1
Tth111I GACn'n_nGTC
Cuts at: 4812 5734 6565 10063 4812
Size: 922 831 3498 7015
Fragments arranged by size:
7015 3498 922 831
XbaI T'CTAG_A
Cuts at: 9297 9297
Size: 12266
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 831 8424 9761 831
Size: 7593 1337 3336
Fragments arranged by size:
7593 3336 1337
XmaI C'CCGG_G
Cuts at: 759 1033 1798 2639 4765 8441 9286 759
Size: 274 765 841 2126 3676 845 3739
Fragments arranged by size:
3739 3676 2126 845 841 765 274
ZraI GAC'GTC
Cuts at: 1067 3195 5518 1067
Size: 2128 2323 7815
Fragments arranged by size:
7815 2323 2128
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AscI AvrII BfrBI BstZ17I Bsu36I EcoRV FspAI NdeI
NruI NsiI PacI PmlI PsiI PspXI SfiI SrfI
SwaI XhoI XmnI