Restriction map for
pF1KSDA1504
(Circular) (Six-base) MAPSORT of: pF1KSDA1504.seq Check: 7831 from: 1 to: 4415
pF1KSDA1504 4415 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 599 1453 599
Size: 854 3561
Acc65I G'GTAC_C
Cuts at: 922 1431 922
Size: 509 3906
AclI AA'CG_TT
Cuts at: 4255 4255
Size: 4415
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2240 2672 3419 3687 2240
Size: 432 747 268 2968
Fragments arranged by size:
2968 747 432 268
AflIII A'CryG_T
Cuts at: 1767 2871 3848 1767
Size: 1104 977 2334
Fragments arranged by size:
2334 1104 977
AgeI A'CCGG_T
Cuts at: 2803 2803
Size: 4415
AlwNI CAG_nnn'CTG
Cuts at: 1720 3287 1720
Size: 1567 2848
ApaLI G'TGCA_C
Cuts at: 3185 3185
Size: 4415
ApoI r'AATT_y
Cuts at: 231 372 1350 1371 1419 3768 4024 231
Size: 141 978 21 48 2349 256 622
Fragments arranged by size:
2349 978 622 256 141 48 21
AseI AT'TA_AT
Cuts at: 20 661 20
Size: 641 3774
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4415
AvaI C'yCGr_G
Cuts at: 1435 1435
Size: 4415
BamHI G'GATC_C
Cuts at: 1440 1440
Size: 4415
BanI G'GyrC_C
Cuts at: 922 1431 1845 2093 2128 922
Size: 509 414 248 35 3209
Fragments arranged by size:
3209 509 414 248 35
BanII G_rGCy'C
Cuts at: 520 1429 2459 520
Size: 909 1030 2476
Fragments arranged by size:
2476 1030 909
BbeI G_GCGC'C
Cuts at: 1849 2097 1849
Size: 248 4167
BbsI GAAGACnn'nnnn_
Cuts at: 283 1176 283
Size: 893 3522
BciVI GTATCCnnnnn_n'
Cuts at: 2313 3074 4415 2313
Size: 761 1341 2313
Fragments arranged by size:
2313 1341 761
BclI T'GATC_A
Cuts at: 1935 1935
Size: 4415
BglII A'GATC_T
Cuts at: 1135 1930 3967 1135
Size: 795 2037 1583
Fragments arranged by size:
2037 1583 795
BlpI GC'TnA_GC
Cuts at: 1527 1527
Size: 4415
Bme1580I G_kGCm'C
Cuts at: 2040 2133 3189 2040
Size: 93 1056 3266
Fragments arranged by size:
3266 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1015 1627 1708 2043 4187 1015
Size: 612 81 335 2144 1243
Fragments arranged by size:
2144 1243 612 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 539 2782 539
Size: 2243 2172
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 671 1059 1307 671
Size: 388 248 3779
Fragments arranged by size:
3779 388 248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1591 2470 2962 3260 1591
Size: 879 492 298 2746
Fragments arranged by size:
2746 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4119 4119
Size: 4415
BsaAI yAC'GTr
Cuts at: 1407 1768 1407
Size: 361 4054
BsaBI GATnn'nnATC
Cuts at: 6 1929 3966 6
Size: 1923 2037 455
Fragments arranged by size:
2037 1923 455
BsaHI Gr'CG_yC
Cuts at: 1044 1846 2094 2796 4292 1044
Size: 802 248 702 1496 1167
Fragments arranged by size:
1496 1167 802 702 248
BsaWI w'CCGG_w
Cuts at: 1604 2125 2803 3077 3224 3635 1604
Size: 521 678 274 147 411 2384
Fragments arranged by size:
2384 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 713 713
Size: 4415
BseYI C'CCAG_C
Cuts at: 157 1840 2598 3175 3671 157
Size: 1683 758 577 496 901
Fragments arranged by size:
1683 901 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1957 2003 3211 104
Size: 1853 46 1208 1308
Fragments arranged by size:
1853 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 520 1429 2207 2397 3189 520
Size: 909 778 190 792 1746
Fragments arranged by size:
1746 909 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1051 1051
Size: 4415
Bsp1286I G_dGCh'C
Cuts at: 520 1429 2040 2133 2207 2397 2459 3189 520
Size: 909 611 93 74 190 62 730 1746
Fragments arranged by size:
1746 909 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3635 3635
Size: 4415
BspHI T'CATG_A
Cuts at: 198 693 987 3591 198
Size: 495 294 2604 1022
Fragments arranged by size:
2604 1022 495 294
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1465 1679 1981 2362 1465
Size: 214 302 381 3518
Fragments arranged by size:
3518 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2707 2761 4245 4410 50
Size: 2657 54 1484 165 55
Fragments arranged by size:
2657 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2327 2327
Size: 4415
BsrFI r'CCGG_y
Cuts at: 2413 2594 2803 2413
Size: 181 209 4025
Fragments arranged by size:
4025 209 181
BsrGI T'GTAC_A
Cuts at: 604 755 604
Size: 151 4264
BssSI C'ACGA_G
Cuts at: 2686 3044 2686
Size: 358 4057
BstAPI GCAn_nnn'nTGC
Cuts at: 1792 1792
Size: 4415
BstBI TT'CG_AA
Cuts at: 110 2776 110
Size: 2666 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1281 1281
Size: 4415
BstYI r'GATC_y
Cuts at: 1 801 1135 1440 1907 1930 2265 2511 3523
Size: 800 334 305 467 23 335 246 1012
Cuts at: 3523 3609 3621 3967 1
Size: 86 12 346 449
Fragments arranged by size:
1012 800 467 449 346 335 334 305
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 587 587
Size: 4415
BtgI C'CryG_G
Cuts at: 1087 1173 1087
Size: 86 4329
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2365 2546 2709 2365
Size: 181 163 4071
Fragments arranged by size:
4071 181 163
BtsI GCAGTG_nn'
Cuts at: 1658 1658
Size: 4415
DraI TTT'AAA
Cuts at: 1414 1414
Size: 4415
DrdI GACnn_nn'nnGTC
Cuts at: 744 2121 2979 744
Size: 1377 858 2180
Fragments arranged by size:
2180 1377 858
EaeI y'GGCC_r
Cuts at: 2000 2174 2565 2592 3937 2000
Size: 174 391 27 1345 2478
Fragments arranged by size:
2478 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2000 2000
Size: 4415
EarI CTCTTCn'nnn_
Cuts at: 914 1087 2438 2648 914
Size: 173 1351 210 2681
Fragments arranged by size:
2681 1351 210 173
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2623 2933 3079 4225 2623
Size: 310 146 1146 2813
Fragments arranged by size:
2813 1146 310 146
EcoICRI GAG'CTC
Cuts at: 518 1427 518
Size: 909 3506
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 671 1059 1307 2240 2672 3419 3687 671
Size: 388 248 933 432 747 268 1399
Fragments arranged by size:
1399 933 747 432 388 268 248
EcoO109I rG'GnC_Cy
Cuts at: 154 1554 154
Size: 1400 3015
EcoRI G'AATT_C
Cuts at: 372 1419 372
Size: 1047 3368
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 307 1049 2434 307
Size: 742 1385 2288
Fragments arranged by size:
2288 1385 742
FspI TGC'GCA
Cuts at: 2196 2196
Size: 4415
HaeII r_GCGC'y
Cuts at: 1849 2097 3119 4098 1849
Size: 248 1022 979 2166
Fragments arranged by size:
2166 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2257 2287 2289 2319 2257
Size: 30 2 30 4353
Fragments arranged by size:
4353 30 30 2
HincII GTy'rAC
Cuts at: 1074 1454 1074
Size: 380 4035
HindIII A'AGCT_T
Cuts at: 950 950
Size: 4415
HpaI GTT'AAC
Cuts at: 1074 1074
Size: 4415
KasI G'GCGC_C
Cuts at: 1845 2093 1845
Size: 248 4167
KpnI G_GTAC'C
Cuts at: 926 1435 926
Size: 509 3906
MluI A'CGCG_T
Cuts at: 3848 3848
Size: 4415
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 508 1111 3086 3270 508
Size: 603 1975 184 1653
Fragments arranged by size:
1975 1653 603 184
MscI TGG'CCA
Cuts at: 2176 2176
Size: 4415
MslI CAynn'nnrTG
Cuts at: 1340 2404 2531 1340
Size: 1064 127 3224
Fragments arranged by size:
3224 1064 127
MspA1I CmG'CkG
Cuts at: 1526 1840 2200 3213 3458 1526
Size: 314 360 1013 245 2483
Fragments arranged by size:
2483 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2596 2596
Size: 4415
NarI GG'CG_CC
Cuts at: 1846 2094 1846
Size: 248 4167
NcoI C'CATG_G
Cuts at: 1087 1173 1087
Size: 86 4329
NgoMIV G'CCGG_C
Cuts at: 2594 2594
Size: 4415
NspI r_CATG'y
Cuts at: 1468 1968 2875 1468
Size: 500 907 3008
Fragments arranged by size:
3008 907 500
PciI A'CATG_T
Cuts at: 2871 2871
Size: 4415
PfoI T'CCnGG_A
Cuts at: 1036 1036
Size: 4415
PmeI GTTT'AAAC
Cuts at: 1414 1414
Size: 4415
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3554 3586 3554
Size: 32 4383
PpuMI rG'GwC_Cy
Cuts at: 154 154
Size: 4415
PsiI TTA'TAA
Cuts at: 782 782
Size: 4415
PstI C_TGCA'G
Cuts at: 1462 2147 1462
Size: 685 3730
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4415
PvuII CAG'CTG
Cuts at: 1840 2200 1840
Size: 360 4055
RsrII CG'GwC_CG
Cuts at: 2610 2610
Size: 4415
SacI G_AGCT'C
Cuts at: 520 1429 520
Size: 909 3506
SalI G'TCGA_C
Cuts at: 1452 1452
Size: 4415
SanDI GG'GwC_CC
Cuts at: 154 154
Size: 4415
SapI GCTCTTCn'nnn_
Cuts at: 2438 2648 2438
Size: 210 4205
SbfI CC_TGCA'GG
Cuts at: 1462 1462
Size: 4415
ScaI AGT'ACT
Cuts at: 3932 3932
Size: 4415
SfcI C'TryA_G
Cuts at: 33 1067 1458 2143 3136 3327 33
Size: 1034 391 685 993 191 1121
Fragments arranged by size:
1121 1034 993 685 391 191
SfoI GGC'GCC
Cuts at: 1847 2095 1847
Size: 248 4167
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4415
SmaI CCC'GGG
Cuts at: 1437 1437
Size: 4415
SmlI C'TyrA_G
Cuts at: 1570 2485 2977 3239 1570
Size: 915 492 262 2746
Fragments arranged by size:
2746 915 492 262
SnaBI TAC'GTA
Cuts at: 1407 1407
Size: 4415
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4415
SphI G_CATG'C
Cuts at: 1468 1968 1468
Size: 500 3915
StuI AGG'CCT
Cuts at: 1389 1389
Size: 4415
StyI C'CwwG_G
Cuts at: 1087 1173 1549 1903 1087
Size: 86 376 354 3599
Fragments arranged by size:
3599 376 354 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1182 1998 2131 2590 2799 1182
Size: 816 133 459 209 2798
Fragments arranged by size:
2798 816 459 209 133
TatI w'GTAC_w
Cuts at: 604 755 3930 604
Size: 151 3175 1089
Fragments arranged by size:
3175 1089 151
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3932 3964 3932
Size: 32 4383
Tth111I GACn'n_nGTC
Cuts at: 2212 2212
Size: 4415
XbaI T'CTAG_A
Cuts at: 1446 1446
Size: 4415
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1910 1910
Size: 4415
XmaI C'CCGG_G
Cuts at: 1435 1435
Size: 4415
XmnI GAAnn'nnTTC
Cuts at: 206 1032 206
Size: 826 3589
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PsiI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI BssHII
BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PshAI PspOMI PspXI PsrI SacII
SexAI SfiI SgrAI SrfI SspI SwaI XhoI ZraI