Restriction map for
pF1KSDA1519
(Circular) (Six-base) MAPSORT of: pF1KSDA1519.seq Check: 1172 from: 1 to: 5078
pF1KSDA1519 5078 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 512 1937 512
Size: 1425 3653
AccI GT'mk_AC
Cuts at: 2116 2116
Size: 5078
Acc65I G'GTAC_C
Cuts at: 2094 2094
Size: 5078
AclI AA'CG_TT
Cuts at: 4918 4918
Size: 5078
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 759 1858 2903 3335 4082 4350 199
Size: 560 1099 1045 432 747 268 927
Fragments arranged by size:
1099 1045 927 747 560 432 268
AflIII A'CryG_T
Cuts at: 1007 2430 3534 4511 1007
Size: 1423 1104 977 1574
Fragments arranged by size:
1574 1423 1104 977
AgeI A'CCGG_T
Cuts at: 3466 3466
Size: 5078
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1459 1459
Size: 5078
AlwNI CAG_nnn'CTG
Cuts at: 348 525 687 1424 1762 1848 1887 1941 2383
Size: 177 162 737 338 86 39 54 442
Cuts at: 2383 3950 348
Size: 1567 1476
Fragments arranged by size:
1567 1476 737 442 338 177 162 86
54 39
ApaLI G'TGCA_C
Cuts at: 499 660 3848 499
Size: 161 3188 1729
Fragments arranged by size:
3188 1729 161
ApoI r'AATT_y
Cuts at: 1501 1776 2082 4431 4687 1501
Size: 275 306 2349 256 1892
Fragments arranged by size:
2349 1892 306 275 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5078
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5078
AvaI C'yCGr_G
Cuts at: 760 1307 1339 1360 1613 2098 760
Size: 547 32 21 253 485 3740
Fragments arranged by size:
3740 547 485 253 32 21
BamHI G'GATC_C
Cuts at: 2103 2103
Size: 5078
BanI G'GyrC_C
Cuts at: 1089 2094 2508 2756 2791 1089
Size: 1005 414 248 35 3376
Fragments arranged by size:
3376 1005 414 248 35
BanII G_rGCy'C
Cuts at: 434 838 1877 2006 2092 3122 434
Size: 404 1039 129 86 1030 2390
Fragments arranged by size:
2390 1039 1030 404 129 86
BbeI G_GCGC'C
Cuts at: 2512 2760 2512
Size: 248 4830
BbsI GAAGACnn'nnnn_
Cuts at: 1375 2017 1375
Size: 642 4436
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1288 1322 1288
Size: 34 5044
BciVI GTATCCnnnnn_n'
Cuts at: 1032 2976 3737 5078 1032
Size: 1944 761 1341 1032
Fragments arranged by size:
1944 1341 1032 761
BclI T'GATC_A
Cuts at: 2598 2598
Size: 5078
BglI GCCn_nnn'nGGC
Cuts at: 1381 2051 1381
Size: 670 4408
BglII A'GATC_T
Cuts at: 2593 4630 2593
Size: 2037 3041
BlpI GC'TnA_GC
Cuts at: 154 209 1592 1942 2190 154
Size: 55 1383 350 248 3042
Fragments arranged by size:
3042 1383 350 248 55
Bme1580I G_kGCm'C
Cuts at: 503 664 996 1214 2703 2796 3852 503
Size: 161 332 218 1489 93 1056 1729
Fragments arranged by size:
1729 1489 1056 332 218 161 93
BmrI ACTGGGnnnn_n'
Cuts at: 2290 2371 2706 4850 2290
Size: 81 335 2144 2518
Fragments arranged by size:
2518 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 816 3445 816
Size: 2629 2449
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 502 940 1414 1582 1870 2010 352
Size: 150 438 474 168 288 140 3420
Fragments arranged by size:
3420 474 438 288 168 150 140
Bpu10I CC'TnA_GC
Cuts at: 186 1225 1469 1514 186
Size: 1039 244 45 3750
Fragments arranged by size:
3750 1039 244 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1990 2254 3133 3625 3923 1990
Size: 264 879 492 298 3145
Fragments arranged by size:
3145 879 492 298 264
BsaI GGTCTCn'nnnn_
Cuts at: 1310 4782 1310
Size: 3472 1606
BsaAI yAC'GTr
Cuts at: 1010 2070 2431 1010
Size: 1060 361 3657
Fragments arranged by size:
3657 1060 361
BsaBI GATnn'nnATC
Cuts at: 6 2592 4629 6
Size: 2586 2037 455
Fragments arranged by size:
2586 2037 455
BsaHI Gr'CG_yC
Cuts at: 2509 2757 3459 4955 2509
Size: 248 702 1496 2632
Fragments arranged by size:
2632 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2267 2788 3466 3740 3887 4298 2267
Size: 521 678 274 147 411 3047
Fragments arranged by size:
3047 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 373 403 1015 1045 1073 1103 1288 1318 1555
Size: 30 612 30 28 30 185 30 237
Cuts at: 1555 1585 373
Size: 30 3866
Fragments arranged by size:
3866 612 237 185 30 30 30 30
30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 175 381 906 909 1309 175
Size: 206 525 3 400 3944
Fragments arranged by size:
3944 525 400 206 3
BseYI C'CCAG_C
Cuts at: 615 1424 2503 3261 3838 4334 615
Size: 809 1079 758 577 496 1359
Fragments arranged by size:
1359 1079 809 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1140 1429 1627 1775 1140
Size: 289 198 148 4443
Fragments arranged by size:
4443 289 198 148
BsiEI CG_ry'CG
Cuts at: 104 534 2620 2666 3874 104
Size: 430 2086 46 1208 1308
Fragments arranged by size:
2086 1308 1208 430 46
BsiHKAI G_wGCw'C
Cuts at: 434 503 664 769 1856 1877 2006 2092 2870
Size: 69 161 105 1087 21 129 86 778
Cuts at: 2870 3060 3852 434
Size: 190 792 1660
Fragments arranged by size:
1660 1087 792 778 190 161 129 105
86 69 21
BsmI GAATG_Cn'
Cuts at: 338 1139 1459 338
Size: 801 320 3957
Fragments arranged by size:
3957 801 320
BsmBI CGTCTCn'nnnn_
Cuts at: 880 1340 1390 880
Size: 460 50 4568
Fragments arranged by size:
4568 460 50
Bsp1286I G_dGCh'C
Cuts at: 434 503 664 769 838 996 1214 1856 1877
Size: 69 161 105 69 158 218 642 21
Cuts at: 1877 2006 2092 2703 2796 2870 3060 3122 3852
Size: 129 86 611 93 74 190 62 730
Cuts at: 3852 434
Size: 1660
Fragments arranged by size:
1660 730 642 611 218 190 161 158
129 105 93 86 74 69 69 62
21
BspEI T'CCGG_A
Cuts at: 4298 4298
Size: 5078
BspHI T'CATG_A
Cuts at: 4254 4254
Size: 5078
BspMI ACCTGCnnnn'nnnn_
Cuts at: 512 1427 1937 2128 2342 2644 3025 512
Size: 915 510 191 214 302 381 2565
Fragments arranged by size:
2565 915 510 381 302 214 191
BsrBI CCG'CTC
Cuts at: 50 182 555 973 3370 3424 4908 5073 50
Size: 132 373 418 2397 54 1484 165 55
Fragments arranged by size:
2397 1484 418 373 165 132 55 54
BsrDI GCAATG_nn'
Cuts at: 725 2990 725
Size: 2265 2813
BsrFI r'CCGG_y
Cuts at: 3076 3257 3466 3076
Size: 181 209 4688
Fragments arranged by size:
4688 209 181
BssSI C'ACGA_G
Cuts at: 433 768 803 3349 3707 433
Size: 335 35 2546 358 1804
Fragments arranged by size:
2546 1804 358 335 35
BstAPI GCAn_nnn'nTGC
Cuts at: 911 1465 1762 1887 2455 911
Size: 554 297 125 568 3534
Fragments arranged by size:
3534 568 554 297 125
BstBI TT'CG_AA
Cuts at: 110 3439 110
Size: 3329 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 946 946
Size: 5078
BstYI r'GATC_y
Cuts at: 1 303 1865 2103 2570 2593 2928 3174 4186
Size: 302 1562 238 467 23 335 246 1012
Cuts at: 4186 4272 4284 4630 1
Size: 86 12 346 449
Fragments arranged by size:
1562 1012 467 449 346 335 302 246
238 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1277 1277
Size: 5078
BtgI C'CryG_G
Cuts at: 125 461 1034 2015 2046 125
Size: 336 573 981 31 3157
Fragments arranged by size:
3157 981 573 336 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1543 3028 3209 3372 1543
Size: 1485 181 163 3249
Fragments arranged by size:
3249 1485 181 163
BtsI GCAGTG_nn'
Cuts at: 1760 2321 1760
Size: 561 4517
DraI TTT'AAA
Cuts at: 2077 2077
Size: 5078
DrdI GACnn_nn'nnGTC
Cuts at: 712 2784 3642 712
Size: 2072 858 2148
Fragments arranged by size:
2148 2072 858
EaeI y'GGCC_r
Cuts at: 674 2663 2837 3228 3255 4600 674
Size: 1989 174 391 27 1345 1152
Fragments arranged by size:
1989 1345 1152 391 174 27
EagI C'GGCC_G
Cuts at: 2663 2663
Size: 5078
EarI CTCTTCn'nnn_
Cuts at: 172 1062 1694 1744 1804 3101 3311 172
Size: 890 632 50 60 1297 210 1939
Fragments arranged by size:
1939 1297 890 632 210 60 50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 947 1651 3286 3596 3742 4888 947
Size: 704 1635 310 146 1146 1137
Fragments arranged by size:
1635 1146 1137 704 310 146
EcoICRI GAG'CTC
Cuts at: 432 1875 2004 2090 432
Size: 1443 129 86 3420
Fragments arranged by size:
3420 1443 129 86
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 352 502 759 940 1414 1582 1858 1870
Size: 153 150 257 181 474 168 276 12
Cuts at: 1870 2010 2903 3335 4082 4350 199
Size: 140 893 432 747 268 927
Fragments arranged by size:
927 893 747 474 432 276 268 257
181 168 153 150 140 12
EcoNI CCTnn'n_nnAGG
Cuts at: 1468 1933 1468
Size: 465 4613
EcoO109I rG'GnC_Cy
Cuts at: 190 744 1202 1262 1439 2217 190
Size: 554 458 60 177 778 3051
Fragments arranged by size:
3051 778 554 458 177 60
EcoRI G'AATT_C
Cuts at: 1501 2082 1501
Size: 581 4497
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 201 3097 201
Size: 2896 2182
FspI TGC'GCA
Cuts at: 2859 2859
Size: 5078
HaeII r_GCGC'y
Cuts at: 1390 2042 2512 2760 3782 4761 1390
Size: 652 470 248 1022 979 1707
Fragments arranged by size:
1707 1022 979 652 470 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 202 234 354 386 1039 1071 1254 1286 1288
Size: 32 120 32 653 32 183 32 2
Cuts at: 1288 1320 1534 1555 1566 1587 2920 2950 2952
Size: 32 214 21 11 21 1333 30 2
Cuts at: 2952 2982 202
Size: 30 2298
Fragments arranged by size:
2298 1333 653 214 183 120 32 32
32 32 32 30 30 21 21 11
2 2
HincII GTy'rAC
Cuts at: 2117 2117
Size: 5078
KasI G'GCGC_C
Cuts at: 2508 2756 2508
Size: 248 4830
KpnI G_GTAC'C
Cuts at: 2098 2098
Size: 5078
MluI A'CGCG_T
Cuts at: 4511 4511
Size: 5078
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1049 1122 3749 3933 1049
Size: 73 2627 184 2194
Fragments arranged by size:
2627 2194 184 73
MscI TGG'CCA
Cuts at: 676 2839 676
Size: 2163 2915
MslI CAynn'nnrTG
Cuts at: 1882 3067 3194 1882
Size: 1185 127 3766
Fragments arranged by size:
3766 1185 127
MspA1I CmG'CkG
Cuts at: 525 902 2189 2503 2863 3876 4121 525
Size: 377 1287 314 360 1013 245 1482
Fragments arranged by size:
1482 1287 1013 377 360 314 245
NaeI GCC'GGC
Cuts at: 3259 3259
Size: 5078
NarI GG'CG_CC
Cuts at: 2509 2757 2509
Size: 248 4830
NcoI C'CATG_G
Cuts at: 125 1034 2015 2046 125
Size: 909 981 31 3157
Fragments arranged by size:
3157 981 909 31
NgoMIV G'CCGG_C
Cuts at: 3257 3257
Size: 5078
NspI r_CATG'y
Cuts at: 2131 2631 3538 2131
Size: 500 907 3671
Fragments arranged by size:
3671 907 500
PciI A'CATG_T
Cuts at: 3534 3534
Size: 5078
PflMI CCAn_nnn'nTGG
Cuts at: 525 525
Size: 5078
PfoI T'CCnGG_A
Cuts at: 231 1391 1868 231
Size: 1160 477 3441
Fragments arranged by size:
3441 1160 477
PmeI GTTT'AAAC
Cuts at: 2077 2077
Size: 5078
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4217 4249 4217
Size: 32 5046
PpuMI rG'GwC_Cy
Cuts at: 744 1202 1262 1439 744
Size: 458 60 177 4383
Fragments arranged by size:
4383 458 177 60
PstI C_TGCA'G
Cuts at: 907 1382 1438 1767 1892 1937 2125 2810 907
Size: 475 56 329 125 45 188 685 3175
Fragments arranged by size:
3175 685 475 329 188 125 56 45
PvuI CG_AT'CG
Cuts at: 104 534 104
Size: 430 4648
PvuII CAG'CTG
Cuts at: 525 902 2503 2863 525
Size: 377 1601 360 2740
Fragments arranged by size:
2740 1601 377 360
RsrII CG'GwC_CG
Cuts at: 3273 3273
Size: 5078
SacI G_AGCT'C
Cuts at: 434 1877 2006 2092 434
Size: 1443 129 86 3420
Fragments arranged by size:
3420 1443 129 86
SalI G'TCGA_C
Cuts at: 2115 2115
Size: 5078
SapI GCTCTTCn'nnn_
Cuts at: 172 3101 3311 172
Size: 2929 210 1939
Fragments arranged by size:
2929 1939 210
SbfI CC_TGCA'GG
Cuts at: 1382 2125 1382
Size: 743 4335
ScaI AGT'ACT
Cuts at: 4595 4595
Size: 5078
SexAI A'CCwGG_T
Cuts at: 1409 1409
Size: 5078
SfcI C'TryA_G
Cuts at: 33 903 1378 1434 1763 1888 1933 2121 2806
Size: 870 475 56 329 125 45 188 685
Cuts at: 2806 3799 3990 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 870 685 475 329 191 188
125 56 45
SfoI GGC'GCC
Cuts at: 2510 2758 2510
Size: 248 4830
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5078
SmaI CCC'GGG
Cuts at: 762 1341 1362 1615 2100 762
Size: 579 21 253 485 3740
Fragments arranged by size:
3740 579 485 253 21
SmlI C'TyrA_G
Cuts at: 2005 2233 3148 3640 3902 2005
Size: 228 915 492 262 3181
Fragments arranged by size:
3181 915 492 262 228
SnaBI TAC'GTA
Cuts at: 2070 2070
Size: 5078
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5078
SphI G_CATG'C
Cuts at: 2131 2631 2131
Size: 500 4578
StuI AGG'CCT
Cuts at: 670 756 985 1282 2062 670
Size: 86 229 297 780 3686
Fragments arranged by size:
3686 780 297 229 86
StyI C'CwwG_G
Cuts at: 125 320 671 931 1034 1214 2015 2046 2212
Size: 195 351 260 103 180 801 31 166
Cuts at: 2212 2566 125
Size: 354 2637
Fragments arranged by size:
2637 801 354 351 260 195 180 166
103 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 271 1040 1333 1525 2661 2794 3253 3462 271
Size: 769 293 192 1136 133 459 209 1887
Fragments arranged by size:
1887 1136 769 459 293 209 192 133
TatI w'GTAC_w
Cuts at: 4593 4593
Size: 5078
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1015 1047 1073 1105 4595 4627 1015
Size: 32 26 32 3490 32 1466
Fragments arranged by size:
3490 1466 32 32 32 26
Tth111I GACn'n_nGTC
Cuts at: 1266 2875 1266
Size: 1609 3469
XbaI T'CTAG_A
Cuts at: 2109 2109
Size: 5078
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 684 2573 684
Size: 1889 3189
XmaI C'CCGG_G
Cuts at: 760 1339 1360 1613 2098 760
Size: 579 21 253 485 3740
Fragments arranged by size:
3740 579 485 253 21
XmnI GAAnn'nnTTC
Cuts at: 407 407
Size: 5078
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BmgBI BmtI BsiWI BsrGI
BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SrfI SspI SwaI
XhoI ZraI