Restriction map for
pF1KSDA1537
(Circular) (Six-base) MAPSORT of: pF1KSDA1537.seq Check: 6786 from: 1 to: 4907
pF1KSDA1537 4907 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AccI GT'mk_AC
Cuts at: 1945 1945
Size: 4907
Acc65I G'GTAC_C
Cuts at: 1923 1923
Size: 4907
AclI AA'CG_TT
Cuts at: 4747 4747
Size: 4907
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 1211 1337 2732 3164 3911 4179 391
Size: 820 126 1395 432 747 268 1119
Fragments arranged by size:
1395 1119 820 747 432 268 126
AflIII A'CryG_T
Cuts at: 2259 3363 4340 2259
Size: 1104 977 2826
Fragments arranged by size:
2826 1104 977
AgeI A'CCGG_T
Cuts at: 3295 3295
Size: 4907
AlwNI CAG_nnn'CTG
Cuts at: 1680 2212 3779 1680
Size: 532 1567 2808
Fragments arranged by size:
2808 1567 532
ApaI G_GGCC'C
Cuts at: 298 298
Size: 4907
ApaLI G'TGCA_C
Cuts at: 3677 3677
Size: 4907
ApoI r'AATT_y
Cuts at: 1732 1778 1911 4260 4516 1732
Size: 46 133 2349 256 2123
Fragments arranged by size:
2349 2123 256 133 46
AseI AT'TA_AT
Cuts at: 20 20
Size: 4907
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4907
AvaI C'yCGr_G
Cuts at: 1927 1927
Size: 4907
BamHI G'GATC_C
Cuts at: 1932 1932
Size: 4907
BanI G'GyrC_C
Cuts at: 1923 2337 2585 2620 1923
Size: 414 248 35 4210
Fragments arranged by size:
4210 414 248 35
BanII G_rGCy'C
Cuts at: 298 1921 2951 298
Size: 1623 1030 2254
Fragments arranged by size:
2254 1623 1030
BbeI G_GCGC'C
Cuts at: 2341 2589 2341
Size: 248 4659
BbsI GAAGACnn'nnnn_
Cuts at: 379 1652 379
Size: 1273 3634
BciVI GTATCCnnnnn_n'
Cuts at: 2805 3566 4907 2805
Size: 761 1341 2805
Fragments arranged by size:
2805 1341 761
BclI T'GATC_A
Cuts at: 2427 2427
Size: 4907
BfrBI ATG'CAT
Cuts at: 1861 1861
Size: 4907
BglII A'GATC_T
Cuts at: 1461 2422 4459 1461
Size: 961 2037 1909
Fragments arranged by size:
2037 1909 961
BlpI GC'TnA_GC
Cuts at: 1621 2019 1621
Size: 398 4509
Bme1580I G_kGCm'C
Cuts at: 298 2532 2625 3681 298
Size: 2234 93 1056 1524
Fragments arranged by size:
2234 1524 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2119 2200 2535 4679 2119
Size: 81 335 2144 2347
Fragments arranged by size:
2347 2144 335 81
BmtI G_CTAG'C
Cuts at: 1036 1036
Size: 4907
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3274 3274
Size: 4907
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 814 1105 1531 814
Size: 291 426 4190
Fragments arranged by size:
4190 426 291
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 492 2083 2962 3454 3752 492
Size: 1591 879 492 298 1647
Fragments arranged by size:
1647 1591 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4611 4611
Size: 4907
BsaAI yAC'GTr
Cuts at: 1899 2260 1899
Size: 361 4546
BsaBI GATnn'nnATC
Cuts at: 6 563 1556 1881 2421 4458 6
Size: 557 993 325 540 2037 455
Fragments arranged by size:
2037 993 557 540 455 325
BsaHI Gr'CG_yC
Cuts at: 2338 2586 3288 4784 2338
Size: 248 702 1496 2461
Fragments arranged by size:
2461 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2096 2617 3295 3569 3716 4127 2096
Size: 521 678 274 147 411 2876
Fragments arranged by size:
2876 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 304 334 335 365 304
Size: 30 1 30 4846
Fragments arranged by size:
4846 30 30 1
BseYI C'CCAG_C
Cuts at: 2332 3090 3667 4163 2332
Size: 758 577 496 3076
Fragments arranged by size:
3076 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2449 2495 3703 104
Size: 2345 46 1208 1308
Fragments arranged by size:
2345 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1921 2699 2889 3681 1921
Size: 778 190 792 3147
Fragments arranged by size:
3147 792 778 190
BsmI GAATG_Cn'
Cuts at: 942 942
Size: 4907
Bsp1286I G_dGCh'C
Cuts at: 298 1921 2532 2625 2699 2889 2951 3681 298
Size: 1623 611 93 74 190 62 730 1524
Fragments arranged by size:
1623 1524 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4127 4127
Size: 4907
BspHI T'CATG_A
Cuts at: 1581 4083 1581
Size: 2502 2405
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1957 2171 2473 2854 1957
Size: 214 302 381 4010
Fragments arranged by size:
4010 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3199 3253 4737 4902 50
Size: 3149 54 1484 165 55
Fragments arranged by size:
3149 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 231 1669 1861 2819 231
Size: 1438 192 958 2319
Fragments arranged by size:
2319 1438 958 192
BsrFI r'CCGG_y
Cuts at: 2905 3086 3295 2905
Size: 181 209 4517
Fragments arranged by size:
4517 209 181
BsrGI T'GTAC_A
Cuts at: 968 968
Size: 4907
BssSI C'ACGA_G
Cuts at: 3178 3536 3178
Size: 358 4549
BstAPI GCAn_nnn'nTGC
Cuts at: 1452 2284 1452
Size: 832 4075
BstBI TT'CG_AA
Cuts at: 110 3268 110
Size: 3158 1749
BstEII G'GTnAC_C
Cuts at: 900 900
Size: 4907
BstYI r'GATC_y
Cuts at: 1 357 465 1461 1932 2399 2422 2757 3003
Size: 356 108 996 471 467 23 335 246
Cuts at: 3003 4015 4101 4113 4459 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 996 471 467 449 356 346 335
246 108 86 23 12
BtgI C'CryG_G
Cuts at: 125 125
Size: 4907
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2857 3038 3201 2857
Size: 181 163 4563
Fragments arranged by size:
4563 181 163
BtsI GCAGTG_nn'
Cuts at: 2150 2150
Size: 4907
ClaI AT'CG_AT
Cuts at: 557 557
Size: 4907
DraI TTT'AAA
Cuts at: 629 1906 629
Size: 1277 3630
DrdI GACnn_nn'nnGTC
Cuts at: 2613 3471 2613
Size: 858 4049
EaeI y'GGCC_r
Cuts at: 174 431 2492 2666 3057 3084 4429 174
Size: 257 2061 174 391 27 1345 652
Fragments arranged by size:
2061 1345 652 391 257 174 27
EagI C'GGCC_G
Cuts at: 2492 2492
Size: 4907
EarI CTCTTCn'nnn_
Cuts at: 788 1604 2930 3140 788
Size: 816 1326 210 2555
Fragments arranged by size:
2555 1326 816 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3115 3425 3571 4717 3115
Size: 310 146 1146 3305
Fragments arranged by size:
3305 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1919 1919
Size: 4907
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 814 1105 1211 1337 1531 2732 3164 3911
Size: 423 291 106 126 194 1201 432 747
Cuts at: 3911 4179 391
Size: 268 1119
Fragments arranged by size:
1201 1119 747 432 423 291 268 194
126 106
EcoO109I rG'GnC_Cy
Cuts at: 294 295 2046 294
Size: 1 1751 3155
Fragments arranged by size:
3155 1751 1
EcoRI G'AATT_C
Cuts at: 1732 1911 1732
Size: 179 4728
EcoRV GAT'ATC
Cuts at: 1095 1095
Size: 4907
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1555 2926 1555
Size: 1371 3536
FspI TGC'GCA
Cuts at: 2688 2688
Size: 4907
HaeII r_GCGC'y
Cuts at: 2341 2589 3611 4590 2341
Size: 248 1022 979 2658
Fragments arranged by size:
2658 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 335 367 398 430 577 609 1471 1503 2749
Size: 32 31 32 147 32 862 32 1246
Cuts at: 2749 2779 2781 2811 335
Size: 30 2 30 2431
Fragments arranged by size:
2431 1246 862 147 32 32 32 32
31 30 30 2
HincII GTy'rAC
Cuts at: 1946 1946
Size: 4907
HindIII A'AGCT_T
Cuts at: 1618 1618
Size: 4907
KasI G'GCGC_C
Cuts at: 2337 2585 2337
Size: 248 4659
KpnI G_GTAC'C
Cuts at: 1927 1927
Size: 4907
MluI A'CGCG_T
Cuts at: 4340 4340
Size: 4907
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 582 3578 3762 582
Size: 2996 184 1727
Fragments arranged by size:
2996 1727 184
MscI TGG'CCA
Cuts at: 2668 2668
Size: 4907
MslI CAynn'nnrTG
Cuts at: 1165 2896 3023 1165
Size: 1731 127 3049
Fragments arranged by size:
3049 1731 127
MspA1I CmG'CkG
Cuts at: 1272 1401 2018 2332 2692 3705 3950 1272
Size: 129 617 314 360 1013 245 2229
Fragments arranged by size:
2229 1013 617 360 314 245 129
NaeI GCC'GGC
Cuts at: 3088 3088
Size: 4907
NarI GG'CG_CC
Cuts at: 2338 2586 2338
Size: 248 4659
NcoI C'CATG_G
Cuts at: 125 125
Size: 4907
NgoMIV G'CCGG_C
Cuts at: 3086 3086
Size: 4907
NheI G'CTAG_C
Cuts at: 1032 1032
Size: 4907
NsiI A_TGCA'T
Cuts at: 1863 1863
Size: 4907
NspI r_CATG'y
Cuts at: 1960 2460 3367 1960
Size: 500 907 3500
Fragments arranged by size:
3500 907 500
PciI A'CATG_T
Cuts at: 3363 3363
Size: 4907
PfoI T'CCnGG_A
Cuts at: 353 830 1547 353
Size: 477 717 3713
Fragments arranged by size:
3713 717 477
PmeI GTTT'AAAC
Cuts at: 1906 1906
Size: 4907
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4046 4078 4046
Size: 32 4875
PspOMI G'GGCC_C
Cuts at: 294 294
Size: 4907
PstI C_TGCA'G
Cuts at: 1260 1373 1954 2639 1260
Size: 113 581 685 3528
Fragments arranged by size:
3528 685 581 113
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4907
PvuII CAG'CTG
Cuts at: 1272 1401 2332 2692 1272
Size: 129 931 360 3487
Fragments arranged by size:
3487 931 360 129
RsrII CG'GwC_CG
Cuts at: 3102 3102
Size: 4907
SacI G_AGCT'C
Cuts at: 1921 1921
Size: 4907
SalI G'TCGA_C
Cuts at: 1944 1944
Size: 4907
SapI GCTCTTCn'nnn_
Cuts at: 1604 2930 3140 1604
Size: 1326 210 3371
Fragments arranged by size:
3371 1326 210
SbfI CC_TGCA'GG
Cuts at: 1954 1954
Size: 4907
ScaI AGT'ACT
Cuts at: 4424 4424
Size: 4907
SexAI A'CCwGG_T
Cuts at: 363 363
Size: 4907
SfcI C'TryA_G
Cuts at: 33 1256 1369 1761 1950 2635 3628 3819 33
Size: 1223 113 392 189 685 993 191 1121
Fragments arranged by size:
1223 1121 993 685 392 191 189 113
SfoI GGC'GCC
Cuts at: 2339 2587 2339
Size: 248 4659
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4907
SmaI CCC'GGG
Cuts at: 1929 1929
Size: 4907
SmlI C'TyrA_G
Cuts at: 471 2062 2977 3469 3731 471
Size: 1591 915 492 262 1647
Fragments arranged by size:
1647 1591 915 492 262
SnaBI TAC'GTA
Cuts at: 1899 1899
Size: 4907
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4907
SphI G_CATG'C
Cuts at: 1960 2460 1960
Size: 500 4407
StuI AGG'CCT
Cuts at: 1124 1124
Size: 4907
StyI C'CwwG_G
Cuts at: 125 2041 2395 125
Size: 1916 354 2637
Fragments arranged by size:
2637 1916 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2490 2623 3082 3291 2490
Size: 133 459 209 4106
Fragments arranged by size:
4106 459 209 133
TatI w'GTAC_w
Cuts at: 968 1038 4422 968
Size: 70 3384 1453
Fragments arranged by size:
3384 1453 70
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4424 4456 4424
Size: 32 4875
Tth111I GACn'n_nGTC
Cuts at: 2704 2704
Size: 4907
XbaI T'CTAG_A
Cuts at: 1938 1938
Size: 4907
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 912 1080 1269 2402 912
Size: 168 189 1133 3417
Fragments arranged by size:
3417 1133 189 168
XmaI C'CCGG_G
Cuts at: 1927 1927
Size: 4907
XmnI GAAnn'nnTTC
Cuts at: 1463 1463
Size: 4907
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NsiI NspI PciI
PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI Bpu10I
BseRI BsgI BsiWI BsmBI BssHII BstXI BstZ17I Bsu36I
CspCI DraIII EcoNI FseI FspAI HpaI MfeI NdeI
NotI NruI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SfiI SgrAI SrfI
SspI SwaI XhoI ZraI