Restriction map for
pF1KSDA1538
(Circular) (Six-base) MAPSORT of: pF1KSDA1538.seq Check: 375 from: 1 to: 6176
pF1KSDA1538 6176 bp
With 182 enzymes: SgfI *
June 20, 2008 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1515 2608 1515
Size: 1093 5083
AatII G_ACGT'C
Cuts at: 221 221
Size: 6176
AccI GT'mk_AC
Cuts at: 919 2729 3214 919
Size: 1810 485 3881
Fragments arranged by size:
3881 1810 485
Acc65I G'GTAC_C
Cuts at: 681 1217 3192 681
Size: 536 1975 3665
Fragments arranged by size:
3665 1975 536
AclI AA'CG_TT
Cuts at: 6016 6016
Size: 6176
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 273 293 634 740 1117 1285 2922 4001 4433
Size: 20 341 106 377 168 1637 1079 432
Cuts at: 4433 5180 5448 273
Size: 747 268 1001
Fragments arranged by size:
1637 1079 1001 747 432 377 341 268
168 106 20
AfeI AGC'GCT
Cuts at: 2544 2544
Size: 6176
AflIII A'CryG_T
Cuts at: 3528 4632 5609 3528
Size: 1104 977 4095
Fragments arranged by size:
4095 1104 977
AgeI A'CCGG_T
Cuts at: 4564 4564
Size: 6176
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2206 2501 2206
Size: 295 5881
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 182 214 182
Size: 32 6144
AlwNI CAG_nnn'CTG
Cuts at: 337 526 657 1519 1888 1987 2229 3481 5048
Size: 189 131 862 369 99 242 1252 1567
Cuts at: 5048 337
Size: 1465
Fragments arranged by size:
1567 1465 1252 862 369 242 189 131
99
ApaI G_GGCC'C
Cuts at: 740 746 1012 1900 3006 740
Size: 6 266 888 1106 3910
Fragments arranged by size:
3910 1106 888 266 6
ApaLI G'TGCA_C
Cuts at: 4946 4946
Size: 6176
ApoI r'AATT_y
Cuts at: 2746 3180 5529 5785 2746
Size: 434 2349 256 3137
Fragments arranged by size:
3137 2349 434 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6176
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6176
AvaI C'yCGr_G
Cuts at: 494 739 1012 1299 1665 2195 2390 2500 2557
Size: 245 273 287 366 530 195 110 57
Cuts at: 2557 3196 494
Size: 639 3474
Fragments arranged by size:
3474 639 530 366 287 273 245 195
110 57
BamHI G'GATC_C
Cuts at: 3201 3201
Size: 6176
BanI G'GyrC_C
Cuts at: 343 626 681 1217 2167 2300 2506 2548 3192
Size: 283 55 536 950 133 206 42 644
Cuts at: 3192 3606 3854 3889 343
Size: 414 248 35 2630
Fragments arranged by size:
2630 950 644 536 414 283 248 206
133 55 42 35
BanII G_rGCy'C
Cuts at: 740 746 1012 1428 1900 2272 2380 2699 2888
Size: 6 266 416 472 372 108 319 189
Cuts at: 2888 3006 3190 4220 740
Size: 118 184 1030 2696
Fragments arranged by size:
2696 1030 472 416 372 319 266 189
184 118 108 6
BbeI G_GCGC'C
Cuts at: 347 630 3610 3858 347
Size: 283 2980 248 2665
Fragments arranged by size:
2980 2665 283 248
BbsI GAAGACnn'nnnn_
Cuts at: 395 1273 1327 395
Size: 878 54 5244
Fragments arranged by size:
5244 878 54
BbvCI CC'TCA_GC
Cuts at: 779 975 2639 779
Size: 196 1664 4316
Fragments arranged by size:
4316 1664 196
BciVI GTATCCnnnnn_n'
Cuts at: 4074 4835 6176 4074
Size: 761 1341 4074
Fragments arranged by size:
4074 1341 761
BclI T'GATC_A
Cuts at: 3696 3696
Size: 6176
BglI GCCn_nnn'nGGC
Cuts at: 635 653 703 819 1432 1471 1497 635
Size: 18 50 116 613 39 26 5314
Fragments arranged by size:
5314 613 116 50 39 26 18
BglII A'GATC_T
Cuts at: 3691 5728 3691
Size: 2037 4139
BlpI GC'TnA_GC
Cuts at: 2008 2492 3288 2008
Size: 484 796 4896
Fragments arranged by size:
4896 796 484
Bme1580I G_kGCm'C
Cuts at: 740 746 1012 1900 2286 2322 2509 3006 3801
Size: 6 266 888 386 36 187 497 795
Cuts at: 3801 3894 4950 740
Size: 93 1056 1966
Fragments arranged by size:
1966 1056 888 795 497 386 266 187
93 36 6
BmgBI CAC'GTC
Cuts at: 2281 2281
Size: 6176
BmrI ACTGGGnnnn_n'
Cuts at: 347 831 882 2695 3388 3469 3804 5948 347
Size: 484 51 1813 693 81 335 2144 575
Fragments arranged by size:
2144 1813 693 575 484 335 81 51
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2676 4543 2676
Size: 1867 4309
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 449 523 2136 2174 2183 2650 449
Size: 74 1613 38 9 467 3975
Fragments arranged by size:
3975 1613 467 74 38 9
Bpu10I CC'TnA_GC
Cuts at: 779 975 1706 2141 2464 2639 2882 779
Size: 196 731 435 323 175 243 4073
Fragments arranged by size:
4073 731 435 323 243 196 175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1345 3032 3352 4231 4723 5021 1345
Size: 1687 320 879 492 298 2500
Fragments arranged by size:
2500 1687 879 492 320 298
BsaI GGTCTCn'nnnn_
Cuts at: 1234 2647 5880 1234
Size: 1413 3233 1530
Fragments arranged by size:
3233 1530 1413
BsaAI yAC'GTr
Cuts at: 1020 1044 1080 3168 3529 1020
Size: 24 36 2088 361 3667
Fragments arranged by size:
3667 2088 361 36 24
BsaBI GATnn'nnATC
Cuts at: 6 3690 5727 6
Size: 3684 2037 455
Fragments arranged by size:
3684 2037 455
BsaHI Gr'CG_yC
Cuts at: 218 344 627 971 3607 3855 4557 6053 218
Size: 126 283 344 2636 248 702 1496 341
Fragments arranged by size:
2636 1496 702 344 341 283 248 126
BsaWI w'CCGG_w
Cuts at: 2840 3365 3886 4564 4838 4985 5396 2840
Size: 525 521 678 274 147 411 3620
Fragments arranged by size:
3620 678 525 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 182 212 1201 1231 3063 3078 3093 3108 182
Size: 30 989 30 1832 15 15 15 3250
Fragments arranged by size:
3250 1832 989 30 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 375 378 381 411 1688 1804 1969 2032 2041
Size: 3 3 30 1277 116 165 63 9
Cuts at: 2041 2050 2062 2065 2077 2080 2083 2086 2095
Size: 9 12 3 12 3 3 3 9
Cuts at: 2095 2253 2589 2707 375
Size: 158 336 118 3844
Fragments arranged by size:
3844 1277 336 165 158 118 116 63
30 12 12 9 9 9 3 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 519 693 754 927 934 1554 1731 1749 2243
Size: 174 61 173 7 620 177 18 494
Cuts at: 2243 2256 2271 2909 3601 4359 4936 5432 519
Size: 13 15 638 692 758 577 496 1263
Fragments arranged by size:
1263 758 692 638 620 577 496 494
177 174 173 61 18 15 13 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2761 2761
Size: 6176
BsiEI CG_ry'CG
Cuts at: 104 2264 2775 3718 3764 4972 104
Size: 2160 511 943 46 1208 1308
Fragments arranged by size:
2160 1308 1208 943 511 46
BsiHKAI G_wGCw'C
Cuts at: 808 1019 2632 3190 3968 4158 4950 808
Size: 211 1613 558 778 190 792 2034
Fragments arranged by size:
2034 1613 792 778 558 211 190
Bsp1286I G_dGCh'C
Cuts at: 740 746 808 1012 1019 1428 1900 2272 2286
Size: 6 62 204 7 409 472 372 14
Cuts at: 2286 2322 2380 2509 2632 2699 2888 3006 3190
Size: 36 58 129 123 67 189 118 184
Cuts at: 3190 3801 3894 3968 4158 4220 4950 740
Size: 611 93 74 190 62 730 1966
Fragments arranged by size:
1966 730 611 472 409 372 204 190
189 184 129 123 118 93 74 67
62 62 58 36 14 7 6
BspEI T'CCGG_A
Cuts at: 5396 5396
Size: 6176
BspHI T'CATG_A
Cuts at: 5352 5352
Size: 6176
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1205 1515 2000 2431 2608 3226 3440 3742 4123
Size: 310 485 431 177 618 214 302 381
Cuts at: 4123 1205
Size: 3258
Fragments arranged by size:
3258 618 485 431 381 310 302 214
177
BsrBI CCG'CTC
Cuts at: 50 2012 2295 2496 2895 2953 4468 4522 6006
Size: 1962 283 201 399 58 1515 54 1484
Cuts at: 6006 6171 50
Size: 165 55
Fragments arranged by size:
1962 1515 1484 399 283 201 165 58
55 54
BsrDI GCAATG_nn'
Cuts at: 1116 1481 2522 4088 1116
Size: 365 1041 1566 3204
Fragments arranged by size:
3204 1566 1041 365
BsrFI r'CCGG_y
Cuts at: 1461 2204 4174 4355 4564 1461
Size: 743 1970 181 209 3073
Fragments arranged by size:
3073 1970 743 209 181
BsrGI T'GTAC_A
Cuts at: 1113 1113
Size: 6176
BssSI C'ACGA_G
Cuts at: 1119 4447 4805 1119
Size: 3328 358 2490
Fragments arranged by size:
3328 2490 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3061 3553 3061
Size: 492 5684
BstBI TT'CG_AA
Cuts at: 110 4537 110
Size: 4427 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1848 2216 1848
Size: 368 5808
BstYI r'GATC_y
Cuts at: 1 3201 3668 3691 4026 4272 5284 5370 5382
Size: 3200 467 23 335 246 1012 86 12
Cuts at: 5382 5728 1
Size: 346 449
Fragments arranged by size:
3200 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 2730 2730
Size: 6176
Bsu36I CC'TnA_GG
Cuts at: 3110 3110
Size: 6176
BtgI C'CryG_G
Cuts at: 201 701 1287 1575 1772 1784 1810 2367 2472
Size: 500 586 288 197 12 26 557 105
Cuts at: 2472 201
Size: 3905
Fragments arranged by size:
3905 586 557 500 288 197 105 26
12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3170 4126 4307 4470 3170
Size: 956 181 163 4876
Fragments arranged by size:
4876 956 181 163
BtsI GCAGTG_nn'
Cuts at: 571 808 1596 1655 2190 2430 2630 3044 3419
Size: 237 788 59 535 240 200 414 375
Cuts at: 3419 571
Size: 3328
Fragments arranged by size:
3328 788 535 414 375 240 237 200
59
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2741 2776 2741
Size: 35 6141
DraI TTT'AAA
Cuts at: 3175 3175
Size: 6176
DraIII CAC_nnn'GTG
Cuts at: 1023 1023
Size: 6176
DrdI GACnn_nn'nnGTC
Cuts at: 153 3882 4740 153
Size: 3729 858 1589
Fragments arranged by size:
3729 1589 858
EaeI y'GGCC_r
Cuts at: 704 1038 1613 1775 1890 2206 2261 2772 2948
Size: 334 575 162 115 316 55 511 176
Cuts at: 2948 3761 3935 4326 4353 5698 704
Size: 813 174 391 27 1345 1182
Fragments arranged by size:
1345 1182 813 575 511 391 334 316
176 174 162 115 55 27
EagI C'GGCC_G
Cuts at: 2261 2772 3761 2261
Size: 511 989 4676
Fragments arranged by size:
4676 989 511
EarI CTCTTCn'nnn_
Cuts at: 214 384 399 417 450 456 716 1093 2039
Size: 170 15 18 33 6 260 377 946
Cuts at: 2039 2054 3095 4199 4409 214
Size: 15 1041 1104 210 1981
Fragments arranged by size:
1981 1104 1041 946 377 260 210 170
33 18 15 15 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1140 1186 4384 4694 4840 5986 1140
Size: 46 3198 310 146 1146 1330
Fragments arranged by size:
3198 1330 1146 310 146 46
EcoICRI GAG'CTC
Cuts at: 3188 3188
Size: 6176
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 273 293 449 523 634 740 1117 1285 2136
Size: 20 156 74 111 106 377 168 851
Cuts at: 2136 2174 2183 2650 2922 4001 4433 5180 5448
Size: 38 9 467 272 1079 432 747 268
Cuts at: 5448 273
Size: 1001
Fragments arranged by size:
1079 1001 851 747 467 432 377 272
268 168 156 111 106 74 38 20
9
EcoNI CCTnn'n_nnAGG
Cuts at: 661 661
Size: 6176
EcoO109I rG'GnC_Cy
Cuts at: 297 498 654 789 1008 1009 1628 1873 1896
Size: 201 156 135 219 1 619 245 23
Cuts at: 1896 1897 2120 2405 2670 3002 3003 3315 297
Size: 1 223 285 265 332 1 312 3158
Fragments arranged by size:
3158 619 332 312 285 265 245 223
219 201 156 135 23 1 1 1
EcoRI G'AATT_C
Cuts at: 3180 3180
Size: 6176
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4195 4195
Size: 6176
FspI TGC'GCA
Cuts at: 3957 3957
Size: 6176
HaeII r_GCGC'y
Cuts at: 347 563 630 944 1974 2546 3610 3858 4880
Size: 216 67 314 1030 572 1064 248 1022
Cuts at: 4880 5859 347
Size: 979 664
Fragments arranged by size:
1064 1030 1022 979 664 572 314 248
216 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2071 2103 4018 4048 4050 4080 2071
Size: 32 1915 30 2 30 4167
Fragments arranged by size:
4167 1915 32 30 30 2
HincII GTy'rAC
Cuts at: 3215 3215
Size: 6176
HindIII A'AGCT_T
Cuts at: 849 2645 849
Size: 1796 4380
KasI G'GCGC_C
Cuts at: 343 626 3606 3854 343
Size: 283 2980 248 2665
Fragments arranged by size:
2980 2665 283 248
KpnI G_GTAC'C
Cuts at: 685 1221 3196 685
Size: 536 1975 3665
Fragments arranged by size:
3665 1975 536
MluI A'CGCG_T
Cuts at: 5609 5609
Size: 6176
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1471 2197 4847 5031 1471
Size: 726 2650 184 2616
Fragments arranged by size:
2650 2616 726 184
MscI TGG'CCA
Cuts at: 706 1777 3937 706
Size: 1071 2160 2945
Fragments arranged by size:
2945 2160 1071
MslI CAynn'nnrTG
Cuts at: 1109 4165 4292 1109
Size: 3056 127 2993
Fragments arranged by size:
3056 2993 127
MspA1I CmG'CkG
Cuts at: 195 373 526 601 754 1759 1908 2260 2275
Size: 178 153 75 153 1005 149 352 15
Cuts at: 2275 2458 2609 3287 3601 3961 4974 5219 195
Size: 183 151 678 314 360 1013 245 1152
Fragments arranged by size:
1152 1013 1005 678 360 352 314 245
183 178 153 153 151 149 75 15
NaeI GCC'GGC
Cuts at: 2206 4357 2206
Size: 2151 4025
NarI GG'CG_CC
Cuts at: 344 627 3607 3855 344
Size: 283 2980 248 2665
Fragments arranged by size:
2980 2665 283 248
NcoI C'CATG_G
Cuts at: 701 1575 1772 701
Size: 874 197 5105
Fragments arranged by size:
5105 874 197
NgoMIV G'CCGG_C
Cuts at: 2204 4355 2204
Size: 2151 4025
NspI r_CATG'y
Cuts at: 2488 3229 3729 4636 2488
Size: 741 500 907 4028
Fragments arranged by size:
4028 907 741 500
PciI A'CATG_T
Cuts at: 4632 4632
Size: 6176
PflMI CCAn_nnn'nTGG
Cuts at: 337 1519 1816 337
Size: 1182 297 4697
Fragments arranged by size:
4697 1182 297
PfoI T'CCnGG_A
Cuts at: 500 2666 500
Size: 2166 4010
PmeI GTTT'AAAC
Cuts at: 3175 3175
Size: 6176
PmlI CAC'GTG
Cuts at: 1020 1044 1020
Size: 24 6152
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 182 214 5315 5347 182
Size: 32 5101 32 1011
Fragments arranged by size:
5101 1011 32 32
PpuMI rG'GwC_Cy
Cuts at: 498 1628 1873 2670 498
Size: 1130 245 797 4004
Fragments arranged by size:
4004 1130 797 245
PshAI GACnn'nnGTC
Cuts at: 1246 1994 1246
Size: 748 5428
PspOMI G'GGCC_C
Cuts at: 736 742 1008 1896 3002 736
Size: 6 266 888 1106 3910
Fragments arranged by size:
3910 1106 888 266 6
PstI C_TGCA'G
Cuts at: 141 875 963 1716 1764 1913 3223 3908 141
Size: 734 88 753 48 149 1310 685 2409
Fragments arranged by size:
2409 1310 753 734 685 149 88 48
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6176
PvuII CAG'CTG
Cuts at: 373 526 601 754 1908 2458 3601 3961 373
Size: 153 75 153 1154 550 1143 360 2588
Fragments arranged by size:
2588 1154 1143 550 360 153 153 75
RsrII CG'GwC_CG
Cuts at: 4371 4371
Size: 6176
SacI G_AGCT'C
Cuts at: 3190 3190
Size: 6176
SalI G'TCGA_C
Cuts at: 3213 3213
Size: 6176
SanDI GG'GwC_CC
Cuts at: 1628 1873 1628
Size: 245 5931
SapI GCTCTTCn'nnn_
Cuts at: 4199 4409 4199
Size: 210 5966
SbfI CC_TGCA'GG
Cuts at: 963 3223 963
Size: 2260 3916
ScaI AGT'ACT
Cuts at: 5693 5693
Size: 6176
SfcI C'TryA_G
Cuts at: 33 137 555 602 871 920 959 1413 1712
Size: 104 418 47 269 49 39 454 299
Cuts at: 1712 1760 1909 3219 3904 4897 5088 33
Size: 48 149 1310 685 993 191 1121
Fragments arranged by size:
1310 1121 993 685 454 418 299 269
191 149 104 49 48 47 39
SfoI GGC'GCC
Cuts at: 345 628 3608 3856 345
Size: 283 2980 248 2665
Fragments arranged by size:
2980 2665 283 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6176
SmaI CCC'GGG
Cuts at: 496 741 1301 2392 2502 2559 3198 496
Size: 245 560 1091 110 57 639 3474
Fragments arranged by size:
3474 1091 639 560 245 110 57
SmlI C'TyrA_G
Cuts at: 1360 3047 3331 4246 4738 5000 1360
Size: 1687 284 915 492 262 2536
Fragments arranged by size:
2536 1687 915 492 284 262
SnaBI TAC'GTA
Cuts at: 3168 3168
Size: 6176
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6176
SphI G_CATG'C
Cuts at: 2488 3229 3729 2488
Size: 741 500 4935
Fragments arranged by size:
4935 741 500
SrfI GCCC'GGGC
Cuts at: 741 1301 741
Size: 560 5616
StyI C'CwwG_G
Cuts at: 701 1575 1772 1796 2438 2534 3310 3664 701
Size: 874 197 24 642 96 776 354 3213
Fragments arranged by size:
3213 874 776 642 354 197 96 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 366 1341 1568 1689 1737 3759 3892 4351 4560
Size: 975 227 121 48 2022 133 459 209
Cuts at: 4560 366
Size: 1982
Fragments arranged by size:
2022 1982 975 459 227 209 133 121
48
TatI w'GTAC_w
Cuts at: 1113 5691 1113
Size: 4578 1598
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 482 514 5693 5725 482
Size: 32 5179 32 933
Fragments arranged by size:
5179 933 32 32
Tth111I GACn'n_nGTC
Cuts at: 1086 3973 1086
Size: 2887 3289
XbaI T'CTAG_A
Cuts at: 3207 3207
Size: 6176
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 758 3671 758
Size: 2913 3263
XmaI C'CCGG_G
Cuts at: 494 739 1299 2390 2500 2557 3196 494
Size: 245 560 1091 110 57 639 3474
Fragments arranged by size:
3474 1091 639 560 245 110 57
ZraI GAC'GTC
Cuts at: 219 219
Size: 6176
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SrfI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AleI AscI AvrII BaeI BcgI BfrBI
BmtI BsiWI BsmI BsmBI BssHII BstEII ClaI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PsiI PspXI PsrI SacII SexAI
SfiI SgrAI SspI StuI SwaI XhoI XmnI